The tumor suppressor p53 and histone deacetylase 1 are antagonistic regulators of the cyclin-dependent kinase inhibitor p21/WAF1/CIP1 gene.

abstract：:We have determined the effects of a number of mutations in the small-t antigen mRNA intron on the alternative splicing pattern of the simian virus 40 early transcript. Expansion of the distance separating the small-t pre-mRNA lariat branch point and the shared large T-small t 3' splice site from 18 to 29 nucleotides (...

journal_title：Molecular and cellular biology

authors： Fu XY,Colgan JD,Manley JL

更新日期：1988-09-01 00:00:00

abstract：:The genes that encode the variable regions of immunoglobulin (Ig) heavy chains are encoded by three DNA segments: VH, D, and JH. During B-cell development these segments are brought together by a pair of site-specific DNA rearrangements. The first of these joins a D segment to a JH segment; the second brings a VH segm...

journal_title：Molecular and cellular biology

authors： Alessandrini A,Desiderio SV

更新日期：1991-04-01 00:00:00

abstract：:The murine int-1 proto-oncogene has been implicated in neural development and, when transcriptionally activated by mouse mammary tumor virus, contributes to the genesis of mammary tumors. To understand the function of the int-1 gene product in these processes, we have characterized the biochemical properties of int-1 ...

journal_title：Molecular and cellular biology

authors： Papkoff J,Schryver B

更新日期：1990-06-01 00:00:00

abstract：:TIN2 is a negative regulator of telomere elongation that interacts with telomeric DNA repeat binding factor 1 (TRF1) and affects telomere length by a telomerase-dependent mechanism. Here we show that inactivation of the mouse TRF1-interacting protein 2 (TIN2) gene results in early embryonic lethality. We further obser...

journal_title：Molecular and cellular biology

authors： Chiang YJ,Kim SH,Tessarollo L,Campisi J,Hodes RJ

更新日期：2004-08-01 00:00:00

abstract：:Transposons have contributed protein coding sequences to a unexpectedly large number of human genes. Except for the V(D)J recombinase and telomerase, all remain of unknown function. Here we investigate the activity of the human SETMAR protein, a highly expressed fusion between a histone H3 methylase and a mariner fami...

journal_title：Molecular and cellular biology

authors： Liu D,Bischerour J,Siddique A,Buisine N,Bigot Y,Chalmers R

更新日期：2007-02-01 00:00:00

abstract：:The steroid hormone progesterone acts via high-affinity nuclear receptors that interact with specific DNA sequences located near the promoter of the hormone-responsive gene. Recent studies suggested that the hormone-occupied progesterone receptor (PR) mediates gene activation by recruiting a cellular coregulatory fact...

journal_title：Molecular and cellular biology

authors： Xu Y,Klein-Hitpass L,Bagchi MK

更新日期：2000-03-01 00:00:00

abstract：:The Drosophila extra sex combs (esc) protein, a member of the Polycomb group (PcG), is a transcriptional repressor of homeotic genes. Genetic studies have shown that esc protein is required in early embryos at about the time that other PcG proteins become engaged in homeotic gene repression. The esc protein consists p...

journal_title：Molecular and cellular biology

authors： Ng J,Li R,Morgan K,Simon J

更新日期：1997-11-01 00:00:00

abstract：:Cells of the human embryonal carcinoma line NEC14 proliferate as densely packed clusters consisting of small, polygonal stem cells and do not express a detectable level of fibronectin (FN). Upon induction of differentiation by treatment with N,N'-hexamethylene bisacetamide (HMBA), the level of FN mRNA increased steepl...

journal_title：Molecular and cellular biology

authors： Suzuki M,Oda E,Nakajima T,Sekiya S,Oda K

更新日期：1998-05-01 00:00:00

abstract：:Transcriptional coactivator with PDZ-binding motif (TAZ) physically interacts with a variety of transcription factors and modulates their activities involved in cell proliferation and mesenchymal stem cell differentiation. TAZ is highly expressed in the kidney, and a deficiency of this protein results in multiple rena...

journal_title：Molecular and cellular biology

authors： Jang EJ,Jeong H,Han KH,Kwon HM,Hong JH,Hwang ES

更新日期：2012-12-01 00:00:00

abstract：:Induction of the prodynorphin gene has been implicated in medium and long-term adaptation during memory acquisition and pain. By 5' deletion mapping and site-directed mutagenesis of the human prodynorphin promoter, we demonstrate that both basal transcription and protein kinase A (PKA)-induced transcription in NB69 an...

journal_title：Molecular and cellular biology

authors： Carrión AM,Mellström B,Naranjo JR

更新日期：1998-12-01 00:00:00

abstract：:To investigate whether the human pro alpha 1(I) collagen chain could form an in vivo functional interspecies heterotrimer with the mouse pro alpha 2(I) collagen chain, we introduced the human COL1A1 gene into Mov13 mice which have a functional deletion of the endogenous COL1A1 gene. Transgenic mouse strains (HucI and ...

journal_title：Molecular and cellular biology

authors： Wu H,Bateman JF,Schnieke A,Sharpe A,Barker D,Mascara T,Eyre D,Bruns R,Krimpenfort P,Berns A

更新日期：1990-04-01 00:00:00

abstract：:A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had ...

journal_title：Molecular and cellular biology

authors： Beck TW,Brennscheidt U,Sithanandam G,Cleveland J,Rapp UR

更新日期：1990-07-01 00:00:00

abstract：:The dynamic SCF (Skp1-cullin1-F-box protein) assembly is controlled by cycles of cullin neddylation/deneddylation based on the deneddylation activity of the COP9 signalosome (CSN) and global sequestration of cullins by CAND1. However, acceptance of this prediction was hampered by the results of recent studies, and the...

journal_title：Molecular and cellular biology

authors： Liu Q,Zhou Y,Tang R,Wang X,Hu Q,Wang Y,He Q

更新日期：2017-11-13 00:00:00

abstract：:Efficient cleavage of both forms of eukaryotic initiation factor 4G (eIF4G-1 and eIF4G-2) has been achieved in HeLa cells by incubation with hybrid proteins containing poliovirus 2Apro. Entry of these proteins into cells is promoted by adenovirus particles. Substantial levels of ongoing translation on preexisting cell...

journal_title：Molecular and cellular biology

authors： Novoa I,Carrasco L

更新日期：1999-04-01 00:00:00

abstract：:There exist in the Xenopus laevis genome clusters of tandemly repeated DNA sequences, consisting of two types of 393-base-pair repeating unit. Each such cluster contains several units of one of these paired tandem repeats (PTR-1), followed by several units of the other repeat (PTR-2). The number of repeats of each typ...

journal_title：Molecular and cellular biology

authors： Carroll D,Garrett JE,Lam BS

更新日期：1984-02-01 00:00:00

abstract：:The scid mouse mutation affects V(D)J rearrangement and double-strand break repair. scid V(D)J rearrangement is characterized by defective coding joint formation which prevents the development of mature B and T cells. Hairpin DNA has been implicated in the formation of V(D)J coding joints. We found scid cells to be pr...

journal_title：Molecular and cellular biology

authors： Staunton JE,Weaver DT

更新日期：1994-06-01 00:00:00

abstract：:A sequence element within pBR322 DNA mediates a cis-acting negative effect on expression from eucaryotic genes in transient expression assays. The negative element overlaps with sequences that inhibit DNA replication, but its effect is observed in the absence of detectable replication of transfected DNA. ...

journal_title：Molecular and cellular biology

authors： Peterson DO,Beifuss KK,Morley KL

更新日期：1987-04-01 00:00:00

abstract：:Approximately half of the introns in Drosophila melanogaster are too small to function in a vertebrate and often lack the pyrimidine tract associated with vertebrate 3' splice sites. Here, we report the splicing and spliceosome assembly properties of two such introns: one with a pyrimidine-poor 3' splice site and one ...

journal_title：Molecular and cellular biology

authors： Talerico M,Berget SM

更新日期：1994-05-01 00:00:00

abstract：:Sequences of DNA that hybridize on Southern blots with cloned intracisternal A-particle (IAP) sequences have been examined in genomic DNAs of neonatal mice, livers of adult mice (3, 6, 12, 18, 24, and 26 months old), and the solid myeloma tumor MOPC-315. The isoschizomers HpaII (CCGG or mCCGG) and MspI (CCGG or CmCGG)...

journal_title：Molecular and cellular biology

authors： Mays-Hoopes LL,Brown A,Huang RC

更新日期：1983-08-01 00:00:00

abstract：:Drosophila melanogaster telomeres have two DNA domains: a terminal array of retrotransposons and a subterminal repetitive telomere-associated sequence (TAS), a source of telomere position effect (TPE). We reported previously that deletion of the 2L TAS array leads to dominant suppression of TPE by stimulating in trans...

journal_title：Molecular and cellular biology

authors： Frydrychova RC,Biessmann H,Konev AY,Golubovsky MD,Johnson J,Archer TK,Mason JM

更新日期：2007-07-01 00:00:00

abstract：:Coordination of skeletal muscle growth and metabolism with nutrient availability is critical for metabolic homeostasis. To establish the role of insulin-like signaling in this process, we used muscle creatine kinase (MCK)-Cre to disrupt expression of insulin receptor substrates Irs1 and Irs2 in mouse skeletal/cardiac ...

journal_title：Molecular and cellular biology

authors： Long YC,Cheng Z,Copps KD,White MF

更新日期：2011-02-01 00:00:00

abstract：:NIH3T3 cells transformed by mouse Int-2/Fgf-3 cDNA express a series of Int-2-related products representing discrete stages of processing and glycosylation. We confirm that in at least two highly transformed clonal lines, Int-2 products acquire further modifications and are efficiently secreted into the culture medium....

journal_title：Molecular and cellular biology

authors： Kiefer P,Peters G,Dickson C

更新日期：1991-12-01 00:00:00

abstract：:Unlike other eukaryotes, the protein-coding genes of Trypanosoma cruzi are arranged in large polycistronic gene clusters transcribed by polymerase II (Pol II). Thus, it is thought that trypanosomes rely solely on posttranscriptional processes to regulate gene expression. Here, we show that the glucosylated thymine DNA...

journal_title：Molecular and cellular biology

authors： Ekanayake DK,Minning T,Weatherly B,Gunasekera K,Nilsson D,Tarleton R,Ochsenreiter T,Sabatini R

更新日期：2011-04-01 00:00:00

abstract：:We employed a protein gel blotting procedure to search for nuclear proteins from rat pituitary cells that bind preferentially to the 5'-flanking region of the rat prolactin gene. By gel blots of chromatin proteins from GH3 rat pituitary tumor cells with a 32P-labeled prolactin genomic clone, we detected two major bind...

journal_title：Molecular and cellular biology

authors： White BA,Preston GM,Lufkin TC,Bancroft C

更新日期：1985-11-01 00:00:00

abstract：:Paramecium tetraurelia contains two types of nuclei, a diploid germinal micronucleus and a large transcriptionally active macronucleus. The macronuclear genome is formed from the micronuclear DNA during sexual reproduction. Previous studies have shown that the processing of the A-type variable surface protein gene dur...

journal_title：Molecular and cellular biology

authors： Scott JM,Mikami K,Leeck CL,Forney JD

更新日期：1994-04-01 00:00:00

abstract：:Keap1 regulates Nrf2 activity in response to xenobiotic and oxidative stresses. Nrf2 is an essential regulator of cytoprotective genes. Keap1-null mice are lethal by weaning age due to malnutrition caused by severe hyperkeratosis of the upper digestive tract. Analysis of Keap1::Nrf2 double mutant mice revealed that cu...

journal_title：Molecular and cellular biology

authors： Taguchi K,Maher JM,Suzuki T,Kawatani Y,Motohashi H,Yamamoto M

更新日期：2010-06-01 00:00:00

abstract：:A set of genes is rapidly inducible when quiescent fibroblasts are stimulated by growth factors or by the activation of temperature-sensitive retroviral protein-tyrosine kinases. Most of these so-called immediate-early genes were cloned by differential cDNA hybridization. DNA sequence analysis identified many of them ...

journal_title：Molecular and cellular biology

authors： Jähner D,Hunter T

更新日期：1991-07-01 00:00:00

abstract：:The tetraspanins are a family of integral membrane proteins with four transmembrane domains. These molecules form multimolecular networks on the surfaces of many different cell types. Gene-targeting studies have revealed a role for tetraspanins in B- and T-lymphocyte function. We have isolated and deleted a novel tetr...

journal_title：Molecular and cellular biology

authors： Tarrant JM,Groom J,Metcalf D,Li R,Borobokas B,Wright MD,Tarlinton D,Robb L

更新日期：2002-07-01 00:00:00

abstract：:We have investigated the mechanism underlying repression of calcitonin/calcitonin gene-related peptide (CT/CGRP) gene expression by retinoic acid. Retinoic acid treatment of the CA77 thyroid C-cell line decreased CT/CGRP promoter activity two- to threefold, which correlates well with the decrease in calcitonin and CGR...

journal_title：Molecular and cellular biology

authors： Lanigan TM,Tverberg LA,Russo AF

更新日期：1993-10-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00