Abstract:
:The KLF6 gene encodes the Krüppel-like factor 6, a transcription factor that has been individualized as a tumor-suppressor gene involved in the regulation of cell proliferation and differentiation. Recently, high frequency (42%) of KLF6 mutations have been reported in colorectal cancers (CRC) as in prostate cancers, astrocytic gliomas and hepatocellular carcinomas. The aims of the study was to confirm the frequency of KLF6 mutations in a larger series of CRC than that previously published by using DNA extracted from frozen tissue samples, which have been proved to generate less mutational artefact than that extracted from formalin-fixed paraffin-embedded tissue samples, in order to compare KLF6 mutation frequency with that of other common genetic alterations and to determine genotype-phenotype correlations. Amplification and direct sequencing of KLF6 exon 2 of 76 CRC and matched normal frozen tissues was performed. Polymorphisms were observed in 14 cases, among which two (T35T and S116S) had not already been reported. No KLF6 somatic mutation was observed. Our data suggest a minor role of KLF6 mutation in colorectal carcinogenesis and underline the fact that the validity of sequence informations obtained from DNA extracted from formalin-fixed tissues may be limited.
journal_name
Oncogenejournal_title
Oncogeneauthors
Lièvre A,Landi B,Côté JF,Veyrie N,Zucman-Rossi J,Berger A,Laurent-Puig Pdoi
10.1038/sj.onc.1208867subject
Has Abstractpub_date
2005-11-03 00:00:00pages
7253-6issue
48eissn
0950-9232issn
1476-5594pii
1208867journal_volume
24pub_type
杂志文章相关文献
ONCOGENE文献大全abstract::Bruton's tyrosine kinase (BTK) is essential for B-cell proliferation/differentiation and it is generally believed that its expression and function are limited to bone marrow-derived cells. Here, we report the identification and characterization of p65BTK, a novel isoform abundantly expressed in colon carcinoma cell li...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2015.504
更新日期:2016-08-18 00:00:00
abstract::Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Since surviving patients experience severe neurocognitive disabilities, better and more effective treatments are needed to enhance their quality of life. Casein kinase 2 (CK2) is known to regulate cell growth and survival in multiple cancers; how...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-019-0927-y
更新日期:2019-10-01 00:00:00
abstract::Myc family proteins play an important role in cellular processes such as proliferation, differentiation, apoptosis and transformation. A number of interaction partners of Myc have been identified, such as Max, p107, TBP, YY1, Miz-1, AP-2 and Nmi. Both Max and Nmi also bind to MycN. In contrast to the well defined bind...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203090
更新日期:1999-11-18 00:00:00
abstract::An elevated proteasome activity contributes to tumorigenesis, particularly by providing cancer cells with antiapoptotic protection and efficient clearance from irregular proteins. Still, the underlying mechanisms are poorly known. In this study, we report that in colon cancer patients, higher proteasome activity was d...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2009.264
更新日期:2009-11-12 00:00:00
abstract::Lysyl oxidase (LOX) is a multifunctional protein required for normal collagen and elastin biosynthesis and maturation. In addition, LOX has complex roles in cancer in which the lysyl oxidase propeptide (LOX-PP) domain of secreted pro-LOX has tumor-suppressor activity, while the active enzyme promotes metastasis. In pr...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.147
更新日期:2015-04-09 00:00:00
abstract::Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a promising candidate for cancer therapy because of its relative tumor selectivity. However, many cancers including pancreatic cancer remain resistant towards TRAIL. To develop TRAIL for cancer therapy of pancreatic carcinoma, it will therefore b...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209776
更新日期:2007-01-11 00:00:00
abstract::Human hematopoietic progenitor cells (TF-1) undergo apoptosis upon deprivation of their dependent cytokine. In this report, we have isolated and characterized some spontaneously derived cytokine-independent variants from TF-1 cells. Analysis of several signaling molecules known to be activated by the GM-CSF pathway re...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1200884
更新日期:1997-02-13 00:00:00
abstract::The transcription factor Snail has been recently proposed as an important mediator of tumour invasion because of its role in downregulation of E-cadherin and induction of epithelial-mesenchymal transitions (EMT). This behaviour has led to the consideration of Snail as a potential therapeutic target to block tumour pro...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209997
更新日期:2007-03-22 00:00:00
abstract::Many lines of evidence indicate that connexin genes expressing gap junction (GJ) proteins inhibit tumor cell proliferation. However, the precise molecular mechanisms remain unclear. In this study, we show that overexpression of connexin43 (Cx43) suppressed proliferation of human osteosarcoma U2OS cells through inhibit...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1204563
更新日期:2001-07-12 00:00:00
abstract::Evasion of apoptosis in pediatric acute lymphoblastic leukemia (ALL) is linked to aberrant expression of inhibitor of apoptosis (IAP) proteins and dysregulated redox homeostasis, rendering leukemic cells vulnerable to redox-targeting therapies. Here we discover that inhibition of antioxidant defenses via glutathione (...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2014.338
更新日期:2015-07-30 00:00:00
abstract::To dissect the p53-dependent apoptotic pathway, events following induction of temperature sensitive (ts) p53val138 were studied in a Ewing tumor cell line. Transcriptional deregulation of p53 targets first observable after 1 h at 32 degrees C preceded activation of caspases and the break-down of mitochondrial respirat...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1203780
更新日期:2000-08-24 00:00:00
abstract::REF cells transformed by oncogenes E1A and cHa-ras reveal high and constitutive DNA-binding activity of AP-1 factor lacking in c-Fos protein. Consistently, the transcription of c-fos gene has been found to be downregulated. To elucidate the mechanisms of c-fos downregulation in E1A+cHa-ras transformants, we studied th...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1205118
更新日期:2002-01-24 00:00:00
abstract::Tyrosine kinase receptors have an essential role in various aspects of tumor progression. In particular, epidermal growth factor receptor (EGFR) and its ligands have been implicated in the growth and dissemination of a wide array of human carcinomas. Here, we describe an EGFR-mediated signaling pathway that regulates ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2011.450
更新日期:2012-05-31 00:00:00
abstract::Upstream stimulating factor (USF2) is a basic helix-loop-helix leucine zipper transcription factor, which is found in most tissues. A critical role for USF2 in cellular proliferation has been proposed based on its importance in the regulation of various cyclins and P53 and its capability to antagonize c-myc. In this p...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1201584
更新日期:1998-02-12 00:00:00
abstract::The p16 gene (MTS1 or CDK4I) encoding an inhibitor of cyclin-dependent kinase 4 (cdk4), has been reported to be deleted in various tumor cell lines, including lines derived from leukemic cells. The reported frequency of p16 gene loss is much higher in established cell lines than in primary tumor specimens. We investig...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1996-05-16 00:00:00
abstract::Fos protein heterodimerizes through one surface of an alpha-helical domain called the leucine zipper. We have investigated the effect of destabilizing this domain by multiply substituting small residues of its non-interacting surface with glycine. Ternary complex formation between mutated Fos, Jun and DNA was determin...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::Myc promotes both normal cell proliferation and oncogenic transformation through the activation and repression of target genes. The c-Myc-S protein is a truncated form of c-Myc that is produced in some cells from translation initiation at an internal AUG codon. We report that c-Myc-S and a similar truncated form of N-...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1210734
更新日期:2008-02-21 00:00:00
abstract::TGF-β is a multifunctional cytokine affecting many cell types and implicated in tissue remodeling processes. Due to its many functions and cell-specific effects, the consequences of TGF-β signaling are process-and stage-dependent, and it is not uncommon that TGF-β exerts distinct and sometimes opposing effects on a di...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.140
更新日期:2017-09-07 00:00:00
abstract::Two alternatively spliced mouse lymphocyte and brain ltk cDNAs predict small transmembrane tyrosine kinases that use CUG translational start codons and that differ upstream of their transmembrane segment. A recently isolated human neuroblastoma ltk cDNA, in contrast, includes a regular AUG start codon and predicts a m...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::To investigate the role of individual Jun proteins in cell growth and transformation, we have used a doxycycline-inducible retroviral vector to regulate their expression in rat fibroblasts. AP-1 complexes enriched with cJun and JunB result in morphological alterations and anchorage-independent cell growth consistent w...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1206644
更新日期:2003-08-28 00:00:00
abstract::Recognition and elimination of malignant cells by cytotoxic T lymphocytes depends on antigenic peptides generated by proteasomes. It has been established that impairment of the immunoproteasome subunits, that is, PSMB8, PSMB9 and PSMB10 (PSMBs), is critical for malignant cells to escape immune recognition. We report h...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2013.224
更新日期:2014-05-22 00:00:00
abstract::Exogenous expression of the catalytic subunit of telomerase, hTERT, in a normal human foreskin fibroblast cell strain resulted in telomerase activity and an extended proliferative lifespan prior to a period of crisis. Three immortalized cell lines with stably maintained telomere lengths were established from cells tha...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1207440
更新日期:2004-04-15 00:00:00
abstract::Growth arrest after u.v. damage was investigated in C3H mouse primary cultured hepatocytes, spontaneously immortalized liver epithelial cells and their H-ras-transformed derivatives. All cells except for one of the transformed lines had the wild type p53 gene considered necessary for the G1-S checkpoint. Growth arrest...
journal_title:Oncogene
pub_type: 杂志文章
doi:
更新日期:1994-10-01 00:00:00
abstract::Birt-Hogg-Dubé syndrome (BHD) is a rare, inherited genodermatosis characterized by hair follicle hamartomas, kidney tumors and spontaneous pneumothorax. The BHD locus was mapped to chromosome 17p11.2 by linkage analysis, and germline mutations in a novel gene (BHD) were identified in a panel of BHD families. Using RNA...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209593
更新日期:2006-09-28 00:00:00
abstract::Metabolic acid production challenges cellular pH homeostasis in solid cancer tissue, and mechanisms of net acid extrusion represent promising new targets for breast cancer therapy. Here, we used genetically engineered mice to investigate the contribution of the Na+,HCO3--cotransporter NBCn1 (Slc4a7) to intracellular a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/s41388-018-0353-6
更新日期:2018-10-01 00:00:00
abstract::Transcriptomic screens in breast cancer cell lines have identified a protein named anterior gradient-2 (AGR2) as a potentially novel oncogene overexpressed in estrogen receptor (ER) positive tumours. As targeting the ER is responsible for major improvements in cure rates and prevention of breast cancers, we have evalu...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2010.228
更新日期:2010-08-26 00:00:00
abstract::P53 wild-type and p53-null or mutant cells undergo a G(2)-phase cell-cycle arrest in response to ionizing radiation (IR). In this study we examined the effect of heat-shock protein 90 (HSP90) inhibitor, geldanamycin (GA), on IR-induced G(2) arrest in human colon adenocarcinoma cells with different p53 status. We show ...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2008.172
更新日期:2008-09-18 00:00:00
abstract::The RASSF8 gene, which maps close to the KRAS2 gene, contains a RAS-associated domain and encodes a protein that is evolutionarily conserved from fish to humans. Analysis of the RASSF8 transcript revealed a complex expression pattern of 5'-UTR mRNA isoforms in normal lung and in lung adenocarcinomas (ADCAs), with no a...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/sj.onc.1209422
更新日期:2006-06-29 00:00:00
abstract::B-cell CLL/lymphoma 6 (BCL6) exerts oncogenic effects in several human hematopoietic malignancies including chronic myeloid leukemia (CML), where BCL6 expression was shown to be essential for CML stem cell survival and self-renewal during imatinib mesylate (IM) treatment. As several lines of evidence suggest that inte...
journal_title:Oncogene
pub_type: 杂志文章
doi:10.1038/onc.2017.85
更新日期:2017-08-10 00:00:00
abstract::Recent advances in the field of in vitro chromatin assembly have led to in vitro transcription systems which reproduce in the test tube, in vivo characteristics of ligand-dependent transcriptional activation by nuclear receptors. Dissection of these systems has begun to provide us with information concerning the under...
journal_title:Oncogene
pub_type: 杂志文章,评审
doi:10.1038/sj.onc.1204329
更新日期:2001-05-28 00:00:00