Abstract：
:Laser-assisted microdissection (LAM) is a powerful tool for isolating specific tissues, cell types and even organelles from sectioned biological specimen in a manner conducive to the extraction of RNA, DNA or protein. LAM, which is an established technique in many areas of biology, has now been successfully adapted for use with plant tissues. Here, we provide an overview of the processes involved in conducting a successful LAM study in plants and review recent developments that have made this technique even more desirable. We also discuss how the technology might be exploited to answer some pertinent questions in plant biology.
journal_name
Trends Plant Scijournal_title
Trends in plant scienceauthors
Day RC,Grossniklaus U,Macknight RCdoi
10.1016/j.tplants.2005.06.006subject
Has Abstractpub_date
2005-08-01 00:00:00pages
397-406issue
8eissn
1360-1385issn
1878-4372pii
S1360-1385(05)00152-4journal_volume
10pub_type
杂志文章abstract：:The members of the pathogenesis-related protein 1 (PR-1) family are among the most abundantly produced proteins in plants on pathogen attack, and PR-1 gene expression has long been used as a marker for salicylic acid-mediated disease resistance. However, despite considerable interest over several decades, their requir...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2017.06.013
更新日期：2017-10-01 00:00:00
abstract：:The cellulose-binding domains (CBDs) in the Phytophthora cellulose-binding elicitor lectin (CBEL) are potent elicitors of plant defence responses. Induction of defence has also been reported in various cellulose-deficient mutants of Arabidopsis thaliana. Based on these observations, we propose a model linking cellulos...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2008.02.004
更新日期：2008-04-01 00:00:00
abstract：:Plants emit a diverse array of phytogenic volatile organic compounds (VOCs). The production and emission of VOCs has been an important area of research for decades. However, recent research has revealed the importance of VOC catabolism by plants and VOC degradation in the atmosphere for plant growth and survival. Spec...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2013.08.011
更新日期：2013-12-01 00:00:00
abstract：:Over the past decade, the ABC model of flower development has been widely promulgated. However, correct flower-organ development requires not only the ABC genes but also the SEPALLATA genes. When the SEPALLATA genes are expressed together with the ABC genes, both vegetative and cauline leaves are converted to floral o...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/s1360-1385(01)01987-2
更新日期：2001-07-01 00:00:00
abstract：:Phyllotaxis, the geometry of leaf arrangement around stems, determines plant architecture. Molecular interactions coordinating the formation of phyllotactic patterns have mainly been studied in multicellular shoot apical meristems of flowering plants. Phyllotaxis evolved independently in the major land plant lineages....
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2020.09.014
更新日期：2021-02-01 00:00:00
abstract：:Current agricultural practice depends on a wide use of pesticides, bactericides, and fungicides. Increased demand for organic products indicates consumer preference for reduced chemical use. Therefore, there is a need to develop novel sustainable strategies for crop protection and enhancement that do not rely on genet...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2015.01.004
更新日期：2015-04-01 00:00:00
abstract：:Plants form diverse symbioses with nitrogen-fixing bacteria to gain access to ammonium, a product of the prokaryote-exclusive enzyme nitrogenase. Improving the symbiotic effectiveness of crop plants like maize, wheat or rice is a highly topical challenge and could help reduce the need for energy-intense nitrogen ferti...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2008.11.009
更新日期：2009-02-01 00:00:00
abstract：:Unless there are mechanisms to selectively retain membrane proteins in the endoplasmic reticulum (ER) or in the Golgi apparatus, they automatically proceed downstream to the plasma or vacuole membranes. Two types of coat protein complex I (COPI)-interacting motifs in the cytosolic tails of membrane proteins seem to fa...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2014.04.004
更新日期：2014-08-01 00:00:00
abstract：:The Arabidopsis genome encodes approximately 20 different mitogen-activated protein kinases (MAPKs) that are likely to be involved in growth, development and responses to endogenous and environmental cues. Several plant MAPKs are activated by a variety of stress stimuli, including pathogen infection, wounding, tempera...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/s1360-1385(01)02103-3
更新日期：2001-11-01 00:00:00
abstract：:Directed evolution (DE) is a powerful approach for generating proteins with new chemical and physical properties. It mimics the principles of Darwinian evolution by imposing selective pressure on a large population of molecules harboring random genetic variation in DNA, such that sequences with specific desirable prop...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2019.08.004
更新日期：2019-11-01 00:00:00
abstract：:Root system architecture (RSA), governed by the phytohormone auxin, endows plants with an adaptive advantage in particular environments. Using geographically representative arabidopsis (Arabidopsis thaliana) accessions as a resource for GWA mapping, Waidmann et al. and Ogura et al. recently identified two novel compon...
journal_title：Trends in plant science
pub_type： 评论,杂志文章
doi：10.1016/j.tplants.2019.12.001
更新日期：2020-02-01 00:00:00
abstract：:Farmers and breeders have long known that often it is the simultaneous occurrence of several abiotic stresses, rather than a particular stress condition, that is most lethal to crops. Surprisingly, the co-occurrence of different stresses is rarely addressed by molecular biologists that study plant acclimation. Recent ...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2005.11.002
更新日期：2006-01-01 00:00:00
abstract：:PHYTOCHROME-INTERACTING FACTOR 3 (PIF3) is a basic helix-loop-helix transcription factor with critical roles in light signaling. Recent work identified PIF3 as a negative regulator of arabidopsis (Arabidopsis thaliana) freezing tolerance. ...
journal_title：Trends in plant science
pub_type： 评论,杂志文章
doi：10.1016/j.tplants.2017.12.003
更新日期：2018-02-01 00:00:00
abstract：:Aquaporins are water channel proteins of vacuolar and plasma membranes. When opened they facilitate the passive movement of water molecules down a water potential gradient. In Arabidopsis, 30 genes have been found that code for aquaporin homologues. Some of these genes code for highly abundant constitutively expressed...
journal_title：Trends in plant science
pub_type： 杂志文章
doi：10.1016/s1360-1385(99)01438-7
更新日期：1999-08-01 00:00:00
abstract：:Metabolism depends on inorganic phosphate (P(i)) as reactant, allosteric effector and regulatory moiety in covalent protein modification. To cope with P(i) shortage (a common situation in many ecosystems), plants activate a set of adaptive responses to enhance P(i) recycling and acquisition by reprogramming metabolism...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2004.09.003
更新日期：2004-11-01 00:00:00
abstract：:The multiple independent origins of plant parasitism suggest that numerous ancestral plant lineages possessed the developmental flexibility to meet the requirements of a parasitic life style, including such adaptations as the ability to recognize host plants, form an invasive haustorium, and regulate the transfer of n...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2010.01.004
更新日期：2010-04-01 00:00:00
abstract：:Recent years have witnessed the genomic decoding of a wide range of photosynthetic organisms from the model plant Arabidopsis thaliana and the complex genomes of important crop species to single-celled marine phytoplankton. The comparative sequencing of green, red and brown algae has provided considerable insight into...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2012.02.005
更新日期：2012-07-01 00:00:00
abstract：:To understand how DNA sequence is translated to phenotype we must understand the epigenetic features that regulate gene expression. Recent research illuminates the complex interactions between DNA methylation, small RNAs, silencing of transposable elements, and genomic imprinting in the Arabidopsis (Arabidopsis thalia...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2010.01.002
更新日期：2010-04-01 00:00:00
abstract：:The formation of specialized metabolites enables plants to respond to biotic and abiotic stresses, but requires the sequential action of multiple enzymes. To facilitate swift production and to avoid leakage of potentially toxic and labile intermediates, many of the biosynthetic pathways are thought to organize in mult...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2014.11.002
更新日期：2015-01-01 00:00:00
abstract：:The plant fungal pathogen Fusarium oxysporum (Fox) is the causal agent of root rot or wilt diseases in several plant species, including crops such as tomato (Solanum lycopersicum), banana (Musa sapientum) and asparagus (Asparagus officinalis). Colonization of plants by Fox leads to the necrosis of the infected tissues...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2007.12.004
更新日期：2008-03-01 00:00:00
abstract：:Through multifaceted genome-scale research involving phylogenomics, targeted gene surveys, and gene expression analyses in diverse basal lineages of angiosperms, our studies provide insights into the most recent common ancestor of all extant flowering plants. MADS-box gene duplications have played an important role in...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2007.06.012
更新日期：2007-08-01 00:00:00
abstract：:Chloroplasts have a dynamic protein environment and, although proteases are presumably major contributors, the identities of these crucial regulatory proteins have only recently been revealed. There are defined proteases within each of the major chloroplast compartments: the ATP-dependent Clp and FtsH proteases in the...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/s1360-1385(02)02326-9
更新日期：2002-10-01 00:00:00
abstract：:Some pri-miRNAs can code for short peptides called micropeptides (miPEPs) and it has been suggested that these peptides positively regulate the accumulation of their associated miRNAs. Recent data further support this model and point towards the potential for miPEPs to be used in the agricultural sector to improve cro...
journal_title：Trends in plant science
pub_type： 杂志文章
doi：10.1016/j.tplants.2020.12.004
更新日期：2020-12-19 00:00:00
abstract：:Although mitogen-activated protein kinase (MAPK) signal transduction cascades are known regulators of various aspects of plant biology, our knowledge of these systems has been largely restricted to a small subset of the MAPKs. However, global analyses are now revealing that many more of these kinases are probably enga...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2009.12.001
更新日期：2010-02-01 00:00:00
abstract：:Acetyl-coenzyme A (CoA) is a central metabolite involved in numerous anabolic and catabolic pathways, as well as in protein acetylation. Beyond histones, a large number of metabolic enzymes are acetylated in both animal and bacteria, and the protein acetylome is now emerging in plants. Protein acetylation is influence...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2012.03.008
更新日期：2012-07-01 00:00:00
abstract：:Although abundant in soils, iron (Fe) is poorly bioavailable for plants. Improving Fe uptake in crops, enabling them to grow in Fe-depleted soils, has become a major focal interest. The secretion of Fe-mobilizing coumarins by plant roots recently emerged as an important factor allowing nongrass species to cope with lo...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2020.10.008
更新日期：2020-11-25 00:00:00
abstract：:Carotenoids are a diverse group of colourful pigments naturally found in plants, algae, fungi and bacteria. They play essential roles in development, photosynthesis, root-mycorrhizal interactions and the production of phytohormones, such as abscisic acid and strigolactone. Carotenoid biosynthesis is regulated througho...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2010.02.003
更新日期：2010-05-01 00:00:00
abstract：:Glycogen synthase kinase 3 (GSK3) proteins, also known as SHAGGY-like kinases, have many important cell signalling roles in animals, fungi and amoebae. In particular, GSK3s participate in key developmental signalling pathways and also regulate the cytoskeleton. GSK3-encoding genes are also present in all land plants a...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2011.10.002
更新日期：2012-01-01 00:00:00
abstract：:The Arabidopsis thaliana accession Landsberg erecta contains an induced mutation in the leucine-rich repeat receptor-like Ser/Thr kinase gene ERECTA. Landsberg erecta is commonly used as a genetic background in mutant screens and in natural variation studies. Therefore, the erecta mutation is present in many loss-of-f...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2009.01.010
更新日期：2009-04-01 00:00:00
abstract：:CURT1 proteins induce membrane curvature to grana margins in Arabidopsis (Arabidopsis thaliana) thylakoids. A domain sharing sequence and structural features with CURT1 is found in some cyanobacterial aminoacyl-tRNA synthetases (aaRSs) that show an unusual localization to the thylakoid membranes. Evolutionary scenario...
journal_title：Trends in plant science
pub_type： 杂志文章,评审
doi：10.1016/j.tplants.2013.12.004
更新日期：2014-02-01 00:00:00
