Activation of hematopoietic progenitor kinase 1 involves relocation, autophosphorylation, and transphosphorylation by protein kinase D1.

abstract：:The upstream promoter sequences of the human carbonic anhydrase II (CA II) gene have been studied by 5' deletion analysis. Promoter activity was assayed by transfection and chloramphenicol acetyltransferase assay in both human HeLa cells and murine L cells. This investigation showed that the CA II promoter is comparab...

journal_title：Molecular and cellular biology

authors： Shapiro LH,Venta PJ,Tashian RE

更新日期：1987-12-01 00:00:00

abstract：:We assayed phosphatidylinositol (PI) kinase (EC 2.7.1.67) activity in detergent extracts of nontransformed or virus-transformed cells. Nontransformed chicken embryo fibroblasts (CEF) contain PI kinase activity with an apparent specific activity of 20 pmol/min per mg of protein. This activity sedimented as a single pea...

journal_title：Molecular and cellular biology

authors： Sugano S,Hanafusa H

更新日期：1985-09-01 00:00:00

abstract：:We have demonstrated that a highly conserved segment of DNA between positions -288 and -317 (upstream sequence element IV [USE IV]) is largely responsible for the transcriptional activation of the sea urchin H1-beta histone gene during the blastula stage of embryogenesis. This sequence is capable of acting as an embry...

journal_title：Molecular and cellular biology

authors： DeAngelo DJ,DeFalco J,Childs G

更新日期：1993-03-01 00:00:00

abstract：:Human MED26 was originally purified in the cofactor required for the Sp1 activation complex (CRSP) as a 70-kDa component named CRSP70. This polypeptide was specific to metazoans and the “small” form of the Mediator complex. We report here that a Drosophila melanogaster homologue of MED26 similarly interacts with other...

journal_title：Molecular and cellular biology

authors： Marr SK,Lis JT,Treisman JE,Marr MT 2nd

更新日期：2014-07-01 00:00:00

abstract：:A mutant has been isolated from the J558 (immunoglobulin A, lambda, anti-alpha 1 leads to 3 dextran) cell line which synthesizes a heavy-chain immunoglobulin twice the size of normal heavy chain. Secondary variants that synthesized heavy chains either 1.5 times as large as wild type or the same size as wild type were ...

journal_title：Molecular and cellular biology

authors： Matsuuchi L,Morrison SL

更新日期：1982-09-01 00:00:00

abstract：:Using a sensitive transfection-tumorigenicity assay, we have isolated a novel transforming gene from the DNA of two patients with chronic myelogenous leukemia. Sequence analysis indicates that the product of this gene, axl, is a receptor tyrosine kinase. Overexpression of axl cDNA in NIH 3T3 cells induces neoplastic t...

journal_title：Molecular and cellular biology

authors： O'Bryan JP,Frye RA,Cogswell PC,Neubauer A,Kitch B,Prokop C,Espinosa R 3rd,Le Beau MM,Earp HS,Liu ET

更新日期：1991-10-01 00:00:00

abstract：:The K-fgf/hst oncogene encodes a secreted growth factor of the fibroblast growth factor (FGF) family. The ability of K-fgf-transformed cells to grow in soft agar and in serum-free medium is inhibited by anti-K-FGF neutralizing antibodies, consistent with an autocrine mechanism of transformation. The transformed proper...

journal_title：Molecular and cellular biology

authors： Talarico D,Basilico C

更新日期：1991-02-01 00:00:00

abstract：:Although the U1 small nuclear ribonucleoprotein particle (snRNP) was the first mRNA-splicing cofactor to be identified, the manner in which it functions in splicing is not precisely understood. Among the information required to understand how U1 snRNP participates in splicing, it will be necessary to know its structur...

journal_title：Molecular and cellular biology

authors： Patton JR,Patterson RJ,Pederson T

更新日期：1987-11-01 00:00:00

abstract：:TRIF is an essential adaptor for Toll-like receptor 3/4 (TLR3/4) signaling to activate transcription factor interferon regulatory factor 3 (IRF-3). We examined the molecular mechanism of TLR3 signaling and found that TLR3 stimulation by double-stranded RNA (dsRNA) induces phosphorylation of TRIF at Ser210 and is requi...

journal_title：Molecular and cellular biology

authors： Abe H,Satoh J,Shirasaka Y,Kogure A,Kato H,Ito S,Fujita T

更新日期：2020-02-12 00:00:00

abstract：:The Capn5 gene was inactivated by homologous recombination in ES cells that subsequently colonized the germ line of mice. The targeted mutation integrated a lacZ expression cassette into the Capn5 gene, allowing the expression of Capn5 mRNA to be examined in detail in heterozygous animals. Expression was observed in e...

journal_title：Molecular and cellular biology

authors： Franz T,Winckler L,Boehm T,Dear TN

更新日期：2004-02-01 00:00:00

abstract：:The Wnt/β-catenin signaling pathway plays crucial roles in early hindbrain formation, and its constitutive activity is associated with a subset of human medulloblastoma, a malignant childhood tumor of the posterior fossa. However, the precise function of Wnt/β-catenin signaling during cerebellar development is still e...

journal_title：Molecular and cellular biology

authors： Lorenz A,Deutschmann M,Ahlfeld J,Prix C,Koch A,Smits R,Fodde R,Kretzschmar HA,Schüller U

更新日期：2011-08-01 00:00:00

abstract：:The Cockayne syndrome B protein (CSB) is required for coupling DNA excision repair to transcription in a process known as transcription-coupled repair (TCR). Cockayne syndrome patients show UV sensitivity and severe neurodevelopmental abnormalities. CSB is a DNA-dependent ATPase of the SWI2/SNF2 family. SWI2/SNF2-like...

journal_title：Molecular and cellular biology

authors： Citterio E,Van Den Boom V,Schnitzler G,Kanaar R,Bonte E,Kingston RE,Hoeijmakers JH,Vermeulen W

更新日期：2000-10-01 00:00:00

abstract：:Inactivation of the tumor suppressor p53 by degradation is a mechanism utilized by cells to adapt to endoplasmic reticulum (ER) stress. However, the mechanisms of p53 destabilization by ER stress are not known. We demonstrate here that the E3 ubiquitin-ligase Hdm2 is essential for the nucleocytoplasmic transport and p...

journal_title：Molecular and cellular biology

authors： Pluquet O,Qu LK,Baltzis D,Koromilas AE

更新日期：2005-11-01 00:00:00

abstract：:We isolated and characterized LP1.2, a mouse myeloma mutant with a deletion of at least 4 kilobases (kb) immediately 3' of the alpha gene and introduction of at least 5 kb of novel (nonimmunoglobulin) sequence in its place. A 6.2-kb genomic EcoRI fragment from the mutated allele was cloned, and a subfragment was seque...

journal_title：Molecular and cellular biology

authors： Gregor PD,Morrison SL

更新日期：1986-06-01 00:00:00

abstract：:Ligand activation of Notch receptors leads to release of the intracellular receptor domain (Notch IC), which translocates to the nucleus and interacts with the DNA-binding protein RBP-Jkappa to control expression of specific target genes. A number of proteins have been shown to interact with Notch ICs and to modulate ...

journal_title：Molecular and cellular biology

authors： Wallberg AE,Pedersen K,Lendahl U,Roeder RG

更新日期：2002-11-01 00:00:00

abstract：:Plasmids carrying the herpes simplex virus thymidine kinase (tk) gene were used to transfect thymidine kinase-deficient cells of the mouse fibroblast cell line LM(tk-). Individual cell clones were cultivated in selective hypoxanthine-aminopterin-thymidine medium to determine the number of integrated plasmid copies whi...

journal_title：Molecular and cellular biology

authors： Pülm W,Knippers R

更新日期：1985-02-01 00:00:00

abstract：:Microinjection of p21Ha-ras proteins into Xenopus laevis oocytes induces a rapid increase of 1,2-diacylglycerol (DAG) levels. The observed alterations in DAG levels were consistent with the ability of the protein to induce maturation, measured by germinal vesicle breakdown (GVBD). Both the increase in DAG levels and G...

journal_title：Molecular and cellular biology

authors： Lacal JC

更新日期：1990-01-01 00:00:00

abstract：:LBP-1 is a cellular protein which binds strongly to sequences around the human immunodeficiency virus type 1 (HIV-1) initiation site and weakly over the TATA box. We have previously shown that LBP-1 represses HIV-1 transcription by inhibiting the binding of TFIID to the TATA box. Four similar but distinct cDNAs encodi...

journal_title：Molecular and cellular biology

authors： Yoon JB,Li G,Roeder RG

更新日期：1994-03-01 00:00:00

abstract：:The ability of the zeste moiety of beta-galactosidase-zeste fusion proteins synthesized in Escherichia coli to bind specific DNA sequences was examined. Such fusion proteins recognize a region of the white locus upstream of the start of transcription; this region has previously been shown to be required for genetic in...

journal_title：Molecular and cellular biology

authors： Mansukhani A,Crickmore A,Sherwood PW,Goldberg ML

更新日期：1988-02-01 00:00:00

abstract：:Oncostatin M is a polypeptide cytokine, produced by normal and malignant hematopoietic cells, that has several in vitro activities, including the ability to inhibit growth of cultured carcinoma cells. Here we present a structural and functional comparison of two oncostatin M-related proteins (Mr 36,000 and 32,000) sec...

journal_title：Molecular and cellular biology

authors： Linsley PS,Kallestad J,Ochs V,Neubauer M

更新日期：1990-05-01 00:00:00

abstract：:The interaction of enhancers with different promoters was studied by measuring the influence of two enhancers (from simian virus 40 and from Harvey sarcoma virus) on the activity of expression vectors that are identical except for their promoter region. The promoters examined were from the simian virus 40 early region...

journal_title：Molecular and cellular biology

authors： Berg PE,Popovic Z,Anderson WF

更新日期：1984-08-01 00:00:00

abstract：:Welander distal myopathy (WDM) is a muscle dystrophy characterized by adult-onset distal muscle weakness, prevalently impacting the distal long extensors of the hands and feet. WDM is an autosomal dominant disorder caused by a missense mutation (c.1362G>A; p.E384K) in the TIA1 (T-cell intracellular antigen 1) gene, wh...

journal_title：Molecular and cellular biology

authors： Carrascoso I,Sánchez-Jiménez C,Silion E,Alcalde J,Izquierdo JM

更新日期：2018-12-11 00:00:00

abstract：:ICP4 of herpes simplex virus is responsible for the activation of viral transcription during infection. It also efficiently activates and represses transcription in vitro depending on the promoter context. The contacts made between ICP4 and the cellular proteins that result in activated transcription have not been ide...

journal_title：Molecular and cellular biology

authors： Carrozza MJ,DeLuca NA

更新日期：1996-06-01 00:00:00

abstract：:Endothelial cells differentiate from mesoderm-derived precursors to initiate the earliest events in vascular development. Although the signaling events that regulate the successive steps of vascular development are known in some detail, the transcriptional processes that regulate the first steps in vasculogenesis are ...

journal_title：Molecular and cellular biology

authors： Wu Y,Moser M,Bautch VL,Patterson C

更新日期：2003-08-01 00:00:00

abstract：:DNA sequencing and blot hybridization analyses have been used to study the structure of a mouse VL30 gene and the molecular nature of VL30-related RNA which is induced upon the stimulation of cultured AKR mouse embryo cells with defined peptide growth factors. An integrated mouse VL30 gene was found to contain identic...

journal_title：Molecular and cellular biology

authors： Hodgson CP,Elder PK,Ono T,Foster DN,Getz MJ

更新日期：1983-12-01 00:00:00

abstract：:p27(Kip1) is a member of the Cip-Kip family of cyclin-dependent kinase (Cdk) inhibitors that binds to cyclin-Cdk complexes and inhibits their catalytic activity in response to antiproliferative stimuli. p27(Kip1) is regulated by several posttranscriptional mechanisms, including subcellular localization. We have identi...

journal_title：Molecular and cellular biology

authors： Smitherman M,Lee K,Swanger J,Kapur R,Clurman BE

更新日期：2000-08-01 00:00:00

abstract：:Scaffold proteins have been established as important mediators of signal transduction specificity. The insulin receptor substrate (IRS) proteins represent a critical group of scaffold proteins that are required for signal transduction by the insulin receptor, including the activation of phosphatidylinositol 3 kinase. ...

journal_title：Molecular and cellular biology

authors： Standen CL,Kennedy NJ,Flavell RA,Davis RJ

更新日期：2009-09-01 00:00:00

abstract：:IMP dehydrogenase (IMPDH) is the rate-limiting enzyme in the de novo synthesis of guanine nucleotides. It is a target of therapeutically useful drugs and is implicated in the regulation of cell growth rate. In the yeast Saccharomyces cerevisiae, mutations in components of the RNA polymerase II (Pol II) transcription e...

journal_title：Molecular and cellular biology

authors： Shaw RJ,Reines D

更新日期：2000-10-01 00:00:00

abstract：:Caveolae are specialized microdomains on membranes that are critical for signal transduction, cholesterol transport, and endocytosis. Caveolin-1 (CAV1) is a multifunctional protein and a major component of caveolae. Cav1 is directly activated by hypoxia-inducible factor (HIF). HIFs are heterodimers of an oxygen-sensit...

journal_title：Molecular and cellular biology

authors： Xie L,Xue X,Taylor M,Ramakrishnan SK,Nagaoka K,Hao C,Gonzalez FJ,Shah YM

更新日期：2014-08-01 00:00:00

abstract：:We previously established that the phage phiC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve...

journal_title：Molecular and cellular biology

authors： Thyagarajan B,Olivares EC,Hollis RP,Ginsburg DS,Calos MP

更新日期：2001-06-01 00:00:00