Abstract:
OBJECTIVE:Cardiac energetics and performance depend on the expression level of the fast (alpha-) and slow (beta-) myosin heavy chain (MHC) isoform. In ventricular tissue, the beta-MHC isoform predominates, whereas in atrial tissue a variable mixture of alpha- and beta-MHC is found. In several cardiac diseases, the slow isoform is upregulated; however, the functional implications of this transition in human myocardium are largely unknown. The aim of this study was to determine the relation between contractile properties and MHC isoform composition in healthy human myocardium using the diversity in atrial tissue. METHODS:Isometric force production and ATP consumption were measured in chemically skinned atrial trabeculae and ventricular muscle strips, and rate of force redevelopment was studied using single cardiomyocytes. MHC isoform composition was determined by one-dimensional SDS-gel electrophoresis. RESULTS:Force development in ventricular tissue was about 5-fold more economical, but nine times slower, than in atrial tissue. Significant linear correlations were found between MHC isoform composition, ATP consumption and rate of force redevelopment. CONCLUSION:These results clearly indicate that even a minor shift in MHC isoform expression has considerable impact on cardiac performance in human tissue.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Narolska NA,van Loon RB,Boontje NM,Zaremba R,Penas SE,Russell J,Spiegelenberg SR,Huybregts MA,Visser FC,de Jong JW,van der Velden J,Stienen GJdoi
10.1016/j.cardiores.2004.09.029subject
Has Abstractpub_date
2005-01-01 00:00:00pages
221-9issue
1eissn
0008-6363issn
1755-3245pii
S0008-6363(04)00432-8journal_volume
65pub_type
杂志文章abstract::The last decade has seen a dramatic increase in the understanding of the molecular basis of arrhythmias. Much of this new information has been driven by genetic studies that focused on rare, monogenic arrhythmia syndromes that were accompanied or followed by cellular electrophysiological or biochemical studies. The ma...
journal_title:Cardiovascular research
pub_type: 杂志文章,评审
doi:10.1016/j.cardiores.2005.04.005
更新日期:2005-08-15 00:00:00
abstract:OBJECTIVE:We recently showed that mitochondrial coupling factor 6 (CF6) is present as a pressor substance and a prostacyclin inhibitor in systemic circulation. However, the regulation mechanism for circulating CF6 is unknown. We investigated the role of tumor necrosis factor-alpha (TNF-alpha) in the generation and rele...
journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/j.cardiores.2004.01.031
更新日期:2004-06-01 00:00:00
abstract:AIMS:Regulated cell death is a main contributor of myocardial ischaemia-reperfusion (IR) injury during acute myocardial infarction. In this context, targeting apoptosis could be a potent therapeutical strategy. In a previous study, we showed that DAXX (death-associated protein) was essential for transducing the FAS-dep...
journal_title:Cardiovascular research
pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
doi:10.1093/cvr/cvr035
更新日期:2011-07-15 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/13.5.297
更新日期:1979-05-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/19.6.326
更新日期:1985-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0008-6363(02)00341-3
更新日期:2002-07-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/j.cardiores.2004.11.029
更新日期:2005-05-01 00:00:00
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journal_title:Cardiovascular research
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doi:10.1093/cvr/cvn087
更新日期:2008-08-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/s0008-6363(99)00142-x
更新日期:1999-08-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:1993-05-01 00:00:00
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doi:10.1093/cvr/cvv151
更新日期:2015-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/cvr/15.6.328
更新日期:1981-06-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1093/cvr/cvn171
更新日期:2008-10-01 00:00:00
abstract::Eighty-one consecutive patients representing a wide clinical spectrum were studied to assess the degree of limitation of the atrial extrastimulus technique for measurement of atrioventricular node refractoriness; and, to determine the relationship between conductivity and functional refractoriness of the atrioventricu...
journal_title:Cardiovascular research
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更新日期:1982-08-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:1990-07-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:2013-10-01 00:00:00
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doi:10.1093/cvr/20.10.740
更新日期:1986-10-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:1990-11-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章,评审
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更新日期:2011-07-15 00:00:00
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journal_title:Cardiovascular research
pub_type: 评论,杂志文章,评审
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更新日期:2000-01-01 00:00:00
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journal_title:Cardiovascular research
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更新日期:2020-10-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/s0008-6363(97)00141-7
更新日期:1997-08-01 00:00:00
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journal_title:Cardiovascular research
pub_type: 杂志文章
doi:10.1016/j.cardiores.2005.02.004
更新日期:2005-06-01 00:00:00