Abstract:
BACKGROUND:Systematic reviews and meta-analyses can help health-care professionals to base their decisions on the highest levels of evidence, and are therefore the cornerstone in practicing evidence-based medicine. In the field of diagnosis in general, and in laboratory medicine in particular, systematic reviews are scarcer, and often of lower quality than in other areas of medicine. AIM:To summarize the principles and methods that can be advised to perform systematic reviews of good quality in laboratory medicine. METHODS:A narrative review of the literature and discussions with members of the Committee on Evidence-Based Laboratory Medicine of the International Federation of Clinical Chemistry and Laboratory Medicine, in particular during our last three meetings, enabled us to adapt to laboratory medicine the methods which are currently being advocated to perform systematic reviews of good quality in other medical areas. RESULTS:The process of systematic reviewing consists of six key steps: 1) preparation: formulation of question(s); 2) systematic search of the literature; 3) selection of primary studies; 4) critical appraisal of the quality of the selected literature; 5) extraction and synthesis of data; 6) interpretation. The main differences between systematic reviews of diagnostic interventions and those of therapeutic interventions bear on question formulation, the choice of study designs to be included or excluded, the assessment of study quality and the statistical methods used to combine their results. The quoted references will help interested readers to deepen by themselves the most technical or controversial points. CONCLUSION:In order to improve quality of care as well as the balance between benefits, harms and costs of practice, each recommendation in guidelines should be based on a systematic review. In addition, such reviews can help to better identify gaps in biomedical knowledge, and improve critical skills, education, and training of professionals. One can therefore reasonably expect that the current multiplication of systematic reviews in laboratory medicine will favor the diffusion of diagnostic technologies with the highest levels of proven efficacy and effectiveness, at the detriment of the others.
journal_name
Ann Biol Clin (Paris)journal_title
Annales de biologie cliniqueauthors
Watine Jsubject
Has Abstractpub_date
2004-11-01 00:00:00pages
611-27issue
6eissn
0003-3898issn
1950-6112journal_volume
62pub_type
杂志文章abstract::Leukotrienes constitute a class of potent biological mediators of inflammation and anaphylaxis. However, their routine assay in various biological fluids is restricted by the complexity of the methodology. Previously this could only be performed by research laboratories with high pressure liquid chromatography and rad...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::According to the French legislation on medical biology (January 16th, 2010), all biological laboratories must be accredited according to ISO 15189 for at least 50% of their activities before the end of 2016. The extraction of DNA from a sample of interest, whether solid or liquid is one of the critical steps in molecu...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2013.0891
更新日期:2013-09-01 00:00:00
abstract::Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired hematopoietic stem cell disorder that must be correctly diagnose because it is a chronic disease with a real impact on the quality of life and the survival of the patients. PNH screening of all patients with anemia or thrombosis is not recommended. We report...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2017.1277
更新日期:2017-10-01 00:00:00
abstract::The authors describe an automatic method of estimation of uric acid based on the colour obtained in the presence of Gaiac resin and peroxidase, and the hydrogen peroxide produced by the action of urate oxidase on uric acid. ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::Anti native DNA antibodies (anti nDNA Ab), which are a highly specific feature of systemic lupus erythematosus (SLE) were measured by 3 methods: an enzyme linked immunosorbent assay (ELISA), an indirect immunofluorescence test on Crithidia luciliae (IFCL) and the Farr assay (reference test). 114 sera from patients wit...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Hemoglobin Alc is the result of non-enzymatic binding of a glucose molecule at the NH2 terminal of the beta chain. Assay of glycosylated hemoglobin is of great utility for the control efficacity of antidiabetic treatments, since such levels provide the clinician with an excellent index of glucose metabolism over the w...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Several biological parameters for assessing selenium status have been determined in years 1985-1986 in a large Belgian population group, males and females 0 to 92 years old, representative from Brussels and surroundings. In 145 people, 20 to 79 years old, mean concentrations were: 1.06 +/- 0.15 mumol Se/l plasma, 5.0 ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::Multidrug resistance (MDR) phenotype expression was evaluated retrospectively in 87 patients with acute myeloid leukemia (AML), 69 with de novo AML, ten with relapsed AML and eight with AML secondary to myelodysplastic syndrome (MDS). MDR phenotype, characterized by P-glycoprotein expression (MRK16 monoclonal antibody...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::High performance liquid chromatography has been used to separate and analyse the different free porphyrins in biological materials. The selective determination of uroporphyrin, coproporphyrin, protoporphyrin and hepta, hexa, pentacarboxylic porphyrins, the sensitive and quantitative fluorescence detection contribute t...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Determination of plasma delta-aminolaevulinic acid levels is of interest for the diagnosis of disorders of heme biosynthesis. We describe a reliable method for the determination of this parameter, based on a modification of the original method of Mauzerall and Granick. Analytical criteria are defined and the reference...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1992-01-01 00:00:00
abstract::48 Tunisian people suffering from the IDDM auto-immune disease were compared to 35 control healthy persons for the polymorphisms of the complement BF, C2 and C4 proteins and genes, of the IgG (Gm allotypes) as well as of the TNF alpha and TCR C beta genes. Our study shows that the BFF1-C4A3-C4BQO and BFS-C4AQ0-C4B1 co...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::The identification of a product absorbed by an opiate consumer is sometimes problematic since there is no specific biomarker for all molecules. We developed an ultra-high pressure liquid chromatography coupled to tandem mass spectrometry technique which allows the identification and the quantification of 25 opiates in...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2014.0944
更新日期:2014-03-01 00:00:00
abstract::The recommended conservative treatment of hyperoxaluria is mainly based on hyperhydration and ingestion of inhibitors of crystallization. In accordance with this context, the aim of this study was to determine the in vitro effect of Hordeum vulgare on calcium oxalate crystallization oxalo-dependent. The crystallizatio...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2012.0770
更新日期:2012-11-01 00:00:00
abstract::We retrospectively evaluated measurement data and clinical relevance of autoantibodies to gangliosides in peripheral neuropathies (PN). The IgG and IgM antiganglioside autoantibodies were determined by our own immunodot-blot assay on membrane and by enzyme-linked immunosorbent assay (Elisa) in sera of 1,342 patients w...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::We have compared three assays for serum galactosyltransferase activity, which use ovomucoïd, asialo agalactofetuin and free N-acetylglucosamine respectively as exogenous acceptors. A very good correlation between the three assays is obtained, for the whole range of GT activity. When the methods are compared to one ano...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The measurement of CDT (Carbohydrate Deficient Transferrin) is an essential biological tool in the diagnosis and follow-up of alcohol abuse. It is also employed as a marker of abstinence for the restitution of driving licences. However, the precision of measurement, and the between laboratory homogeneity of the result...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,多中心研究
doi:
更新日期:2007-03-01 00:00:00
abstract::We resume here the guidelines about sample processing and transport for laboratory tests. All the steps of the pre-examination phase process, downstream to the recording of the requests, are described in this document. The purpose is to assure a secure and optimal sample management. The traceability of all the steps o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,实务指引
doi:10.1684/abc.2011.0549
更新日期:2010-12-01 00:00:00
abstract::The authors have studied several seric, plasmatic and urinary constituents in patients hospitalized for an acute abdominal syndrome to be able to characterize an eventual pancreatic lesion; mainly seric and urinary amylase as well as its isoenzymes, lipase, liver profile and trypsin. In acute pancreatitis, the means o...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The great diversity of methods for manganese analysis in biological materials (serum, plasma, whole blood, urine, spinal fluid and hair) reveals the difficulty in measuring extremely small quantities of this element. An examination of these methods demonstrate that the most used one is flameless atomic absorption spec...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:1988-01-01 00:00:00
abstract::HbA(1c) assay by high pressure liquid chromatography remains submitted to interferences, among which that of labile HbA(1c) in 1 to 2% of samples. We have evaluated the interference of labile HbA(1c) on HbA(1c) assay using Variant II analyzer (Biorad), by in vitro formation of labile glycated haemoglobin and by evalua...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2008.0251
更新日期:2008-07-01 00:00:00
abstract::The simultaneous presence of homogeneous and heterogeneous reactions at different binding sites of a multiepitope antigen makes the description of the kinetic parameters of the so called "one step" solid phase immunometric assays complex. The authors extended the "one step" approach to the concept of the "soluble sand...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::International guidelines emphasize the importance of LDL cholesterol (LDL-C) assay in the care and follow-up of patients with cardiovascular risk. Most studies and common practice use Friedewald's formula for LDL-C calculation. The accuracy of the result depends closely on the precision of the input parameters (total ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,多中心研究
doi:
更新日期:2005-01-01 00:00:00
abstract::Con A stimulated lymphocytes from some patients with primary glomerular disease release in vitro a lymphokine, the vascular permeability factor (VPF). House dust sensitization was early reported among these patients. We therefore studied the action of this specific mitogen on VPF production in 58 patients with primary...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Myoadenylate deaminase deficit (MAD, MIM#615511) is the most common cause of metabolic myopathies with an estimated prevalence of 1-2% in the general population. We report the case of a 39-year-old man suffering from severe skeletal muscle pain that had developed gradually for 4 years. A moderate increase in creatine ...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2017.1253
更新日期:2017-08-01 00:00:00
abstract::We report the case of an 83-year-old woman admitted to the accident and emergency unit for pneumopathy. The blood cell count on the DXH automaton (Coulter® ™ UniCel® DxH) found a normochromic and normocytic anemia, anormal platelet count and subnormal leukocyte formula. The only alarm raised by the automaton was the p...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:10.1684/abc.2018.1406
更新日期:2019-04-01 00:00:00
abstract::S100B is a small protein selectively synthesized by cerebral astroglial cells. S100B participates physiologically in the regulation of intracellular free calcium levels, and exerts a neurotrophic activity on cerebral cells. The interest of S100B protein in clinical biology results from its physiological presence in bi...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2013.0901
更新日期:2013-11-01 00:00:00
abstract::Sixty-three strains of Pseudomonas paucimobilis, a Gram-negative, lemon yellow pigmented, glucose nonfermenting bacillus are phenotypically compared to the type strain of this new species (ATCC 29837). The morphological, cultural, biochemical (ONPG-test, oxidase, aesculin positive) and nutritional (assimilation of man...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1986-01-01 00:00:00
abstract::Human embryonic stem cells (hESC) are obtained from the inner cell mass from the early embryo at blastocyste stage. Derived in cell lines for the first time in 1998, they can be maintained in culture in an undifferentiated state indefinitely under certain conditions. Two essential properties characterize hESC: pluripo...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:10.1684/abc.2008.0224
更新日期:2008-05-01 00:00:00
abstract::Caveolae are small pockets or invaginations localized at the plasma membrane. They are enriched in glycosphingolipids, cholesterol, sphingomyelin and lipid-enchored membrane proteins, and they are characterized by a light buoyant density and resistance to solubilization by Triton X-100 at 4 C. Caveolins are the princi...
journal_title:Annales de biologie clinique
pub_type: 杂志文章,评审
doi:
更新日期:2000-03-01 00:00:00
abstract::Free and total tryptophan is estimated in the plasma by a spectrofluorimetric method using an amplification reaction and fluorescence by transformation of tryptophan into norharman using formalin and perhydrol (fluorescence is amplified about 15 times). Free tryptophan in the plasma is separated by ultrafiltration und...
journal_title:Annales de biologie clinique
pub_type: 杂志文章
doi:
更新日期:1977-01-01 00:00:00