Abstract：
:Twenty-four male volunteers (mean +/- SD: age 25.4+/-5.8 years, height 178.6+/-5.5 cm, body mass 72.1+/-7.7 kg) of different training background were investigated and classified into three groups according to their physical activity and sport discipline: untrained students (group A), national and sub-national level endurance athletes (group B, 7.8+/-2.9 years of specialised training) and sprint-power athletes (group C, 12.8+/-8.7 years of specialised training). Muscle biopsies of vastus lateralis were analysed histochemically for mATPase and SDH activities, immunohistochemically for fast and slow myosin, and electrophoretically followed by Western immunoblotting for myosin heavy chain (MyHC) composition. Significant differences (P<0.05) regarding composition of muscle fibre types and myosin heavy chains were found only between groups A (41.7+/-1.6% of MyHCI, 40.8+/-4.0% of MyHCIIA and 17.5+/-4.0% of MyHCIIX) and B (64.3+/-0.8% of MyHCI, 34.0+/-1.4% of MyHCIIA and 1.7+/-1.4% of MyHCIIX) and groups A and C (59.6+/-1.6% of MyHCI, 37.2+/-1.3% of MyHCIIA and 3.2+/-1.3% of MyHCIIX). Unexpectedly, endurance athletes (group B) such as long-distance runners, cyclists and cross country skiers, did not differ from the athletes representing short term, high power output sports (group C) such as ice hockey, karate, ski-jumping, volleyball, soccer and modern dance. Furthermore, the relative amount of the fastest MyHCIIX isoform in vastus lateralis muscle was significantly lower in the athletes from group C than in students (group A). We conclude that the myosin profile in the athletes belonging to group C was unfavourable for their sport disciplines. This could be the reason why those athletes did not reach international level despite of several years of training.
journal_name
Folia Histochem Cytobioljournal_title
Folia histochemica et cytobiologicaauthors
Zawadowska B,Majerczak J,Semik D,Karasinski J,Kolodziejski L,Kilarski WM,Duda K,Zoladz JAsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
181-90issue
3eissn
0239-8508issn
1897-5631journal_volume
42pub_type
杂志文章abstract：:Histochemical and immunohistochemical methods should often but not always complement standardized histopathologic procedures. Here, we illustrate use of these ancillary techniques in a report of scrotal leiomyosarcoma. 62-year-old male patient presented with a palpable, subcutaneous 2,5 cm wide tumor arising from dart...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.2013.0046
更新日期：2013-01-01 00:00:00
abstract：:The intra-S-phase checkpoint response to hydroxyurea (HU)-mediated arrest of DNA replication was analysed in root meristems of two legumes, Pisum sativum and Vicia faba. The obtained results suggest that a molecular signal which invokes mechanisms allowing the cells to override the S-M dependency control system may be...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Double fluorescence staining with o-phthalaldehyde (OPT) and propidium diiodide (PI) was applied to measure the nuclear protein SH/DNA ratio in species differing in 2C DNA content. It has been shown that protein SH/DNA ratio decreases along with the increase in 2C DNA content. In endosperm, this ratio is higher than i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:Platinum-based chemotherapy is the main type of I-line treatment of advanced and non-operative NSCLC patients without EGFR gene mutation. The excision repair cross-complementation group 1 (ERCC1) is an enzyme that executes the incision of the damaged DNA strand and removes platinum-induced DNA adducts. We investigated...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/18700
更新日期：2012-04-24 00:00:00
abstract：:Dendritic cells (DCs) are regarded as the most potent antigen presenting cells that are well suited to activate T cells toward various antigens, such as tumor-associated antigens, due to their costimulatory activity. There is evidence that DCs are of diverse origin, with at least two types of myeloid and lymphoid prec...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Using immunohistochemistry and Western blot analysis we attempted to identify the estrogen receptors in ovine luteal cells at different stages of the estrous cycle. Monoclonal antibody against estrogen receptors was used for immunolocalization of estrogen receptor-alpha in corpora lutea sections. Generally, the most i...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2000-01-01 00:00:00
abstract：INTRODUCTION:Sucla2, a β subunit of succinyl coenzyme A synthase, is located in the mitochondrial matrix. Sucla2 catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. We ha...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/FHC.a2016.0020
更新日期：2016-01-01 00:00:00
abstract：:Fertilization involves a series of cellular interactions culminating in the fusion of gamete membranes, creating a zygote and then an embryo. During the process of human fertilization in vivo or in conventional in vitro fertilization (IVF), sperm must be capable of undergoing the acrosome reaction, binding to the zona...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The ability to preselect the sex of human offspring would have a significant impact on the preventing of sex chromosome-linked diseases and on human reproduction. Numerous methods have been reviewed including: Sephadex gel filtration, centrifugation in density gradients, separation in albumin gradient, laminar flow, c...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1995-01-01 00:00:00
abstract：:The aim of this study was to compare the imaging symptoms and microscopic findings in females with lobular neoplasia (LN) found on biopsy. 1,478 women who underwent primary open biopsy or surgical excision after percutaneous biopsy were reviewed. In 24 of them (1.6%), LN was found. In four patients, excisional biopsy ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0059
更新日期：2011-01-01 00:00:00
abstract：:The effect of limited amino acid nutrition upon the growth of B16 melanoma cells was investigated. It was observed that the cells in Basal Medium Eagle do not proliferate and are inhibited predominantly in the G1 phase of cell cycle. The cells which were inhibited in growth for 7 or 12 days in Basal Medium Eagle were ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:The aim of the study was to evaluate the influence of a nitric oxide donor: Glyco-2-SNAP on the proliferation status of two breast cancer cell lines: MDA-MB-231 and MCF-7. The study was performed by the thymidine incorporation method as well as by the immunocytochemical detection of the Ki-67 antigen. We found that th...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The study aimed at examination of tissue expression of polysaccharides and secretory mucin 5AC (MUC5AC) in young patients (up to 25 years of age) with a symptomatic gallstones. For comparison, patients most frequently subjected to cholecystectomy were studied, i.e. patients of approximately 50 years of age with the sa...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0053-8
更新日期：2010-12-01 00:00:00
abstract：:Therapeutic outcomes of definitively treated non-small-cell lung cancer (NSCLC) are unacceptably poor. It has been proposed that the manipulation of dendritic cells (DCs) as a "natural" vaccine adjuvant may prove to be a particularly effective way to stimulate antitumor immunity. Presently, there is no standardized me...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0078-4
更新日期：2008-01-01 00:00:00
abstract：:The structure of epidermis and appearance of keratinocytes is described in intact skin specimens from human corpses stored after death under refrigeration. Two groups of alterations can be identified depending on the epidermal layer. In the spinous layer, the cells are characterized by crescent-shaped nuclei surrounde...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:We studied the correlation between the cytotoxic effect of hamster peritoneal macrophages from animals bearing transplantable melanomas of common origin but differing in many biological features on the cells of those melanomas and the sensitivity of the melanoma cells to exogenous OSM, TNF-alpha and IL6. Our results d...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:A comparison of the number of mRNA molecules of histone H3 in 1 microg total RNA extracted from colon sections sampled during colonoscopy was used to evaluate cellular proliferation activity in the rectum and sigmoid, in normal tissue and in colitis ulcerosa. Samples with similar intensity of the disease were selected...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:The frequency of Helicobacter pylori infection in population can depend on the organism resistance, genetic condition, and bacterial strains virulence. A vacA gene, of mosaic structure, which encodes vacuolating cytotoxin is one of the known genes of H. pylori. The existence of several different genotypes of s and m r...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:It was demonstrated by a cytochemical method that the calcium-dependent ATPase was present in the germinating pollen grain. Immediately after hydration the enzyme was observed in the pollen plasmalemma. During germination, the chief sites of the enzyme activity were the plasmalemma around the aperture site and the cis...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:A child was operated 3 times because of a recurrent growth hormone- and prolactin-producing pituitary adenoma. Between the operations she was treated for five years with bromocriptine. The characteristics of the tumour cell population collected after the last operation was now examined by electron microscopy, immunocy...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1997-01-01 00:00:00
abstract：:We have investigated immunohistochemically the effect of dl-alpha-tocopherol (vitamin E) on thyroid gland with 6-n-propyl-2-thiouracil (PTU)-induced hypothyroidism in rats. The animals were divided into four groups. Rats in group I were designated as control, rats in group II were treated with injections of PTU (10 mg...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Delayed type hypersensitivity reaction (DTH) consists of a sequential cascade of steps depending on different types of T cells, as well as mast cells, endothelial cells and macrophages. Recently it has been shown that CD4+ TH1 lymphocytes ("inflammatory type") play a central role in DTH reaction. Activated TH1 cells p...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章,评审
doi：
更新日期：1990-01-01 00:00:00
abstract：:The effectiveness of the long acting somatostatin analogues like octreotide and lanreotide depends on the expression of specific somatostatin receptors on the target cells. The immunohistochemical method performed on surgically removed tumors searches the expression of receptors at the level of receptor protein and gi...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-008-0103-7
更新日期：2010-01-01 00:00:00
abstract：:The aim of the study was to evaluate the prognostic value of the vascular endothelial growth factor A (VEGF-A) and hepatocyte growth factor receptor (HGFR, c-met) expressions in homogenous group of breast cancer patients. Tumor samples were collected from 98 patients with invasive ductal breast carcinoma stage II trea...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-010-0016-0
更新日期：2010-01-01 00:00:00
abstract：:Diplotene oocyte nucleus of the scorpionfly Panorpa communis is transcriptionally silent and contains numerous nuclear bodies including interchromatin granule clusters (IGCs). The latter consist of the granules of 30-50 nm in diameter and contain IGC marker protein SC35 as well as RNA polymerase II. In this study, we ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.2478/v10042-009-0006-2
更新日期：2009-01-01 00:00:00
abstract：:Microtubules present in the epidermis of Ornithogalum umbellatum ovary in the area of lipotubuloids (i.e. aggregates of lipid bodies surrounded by microtubules) are 25-51 nm in diameter. They consist mainly of 10 and 11, sometimes 9 and 12 protofilaments. An average diameter of microtubule consisting of 9 subunits is ...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Differentiation of the lingual taste discs (TDs) was studied in tadpoles of Rana esculenta, Hyla arborea and Pelobatesfuscus by means of LM, TEM, and SEM. The process of differentiation of mucous cells (MCs) within a TD anlage was investigated subsequently from 39th to 46th Gosner's developmental stage. In R. esculent...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The supramolecular dye Congo red was used to check whether monocyte activation may be mediated by a torsion-dependent mechanism preventing transduction of weak random signals in cell contacts in a way corresponding to the discrimination mechanism found in complement fixation by immune complexes. Tight cell-cell contac...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：2003-01-01 00:00:00
abstract：:Lipotubuloids, structures containing lipid bodies and microtubules, are described in ovary epidermal cells of Ornithogalum umbellatum. Microtubules of lipotubuloids can be fixed in electron microscope fixative containing only buffered OsO(4) or in glutaraldehyde with OsO(4) post-fixation, or in a mixture of OsO(4) and...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：10.5603/fhc.2011.0040
更新日期：2011-01-01 00:00:00
abstract：:Three proteinases from the azurophilic granules of horse leucocytes are typical elastases degrading elastin at neutral pH. Both proteinases: 1 and 2A exhibit similar elastinolytic activity, comparable with human leucocyte elastase (HLE). In relation to human enzyme, elastase 2B shows several-fold higher activity, whic...
journal_title：Folia histochemica et cytobiologica
pub_type： 杂志文章
doi：
更新日期：1986-01-01 00:00:00
