Abstract：
BACKGROUND/AIMS:The use of cell lines in bioartificial liver support systems (BALSS) to treat fulminant hepatic failure (FHF) is hindered by their reduced metabolic functions, which could be further decreased by the patient's serum/plasma. Hence, the aim of this study was to (i) test the effect of the FHF serum on C3A cell metabolism; (ii) precondition the cells to improve their metabolic capacity. METHODS:C3A cells were preconditioned in a medium developed at the University of Edinburgh (UoE) or a 10% FHF serum medium. Metabolism capacity was assessed on days 3, 7 and 10 and compared with primary porcine hepatocytes. Preconditioned-cell metabolism was reassessed after (i) passage and (ii) incubation with 10% FHF serum. RESULTS:UoE-preconditioned cells showed time-dependent increase in gluconeogenesis (500%), ureogenesis (200%), galactose elimination (240%) albumin synthesis (250%). These results were in the same order of magnitude as the ones obtained with primary porcine hepatocytes and were further enhanced by cell passage. UoE-preconditioning prevented the decrease of metabolism induced by acute incubation with FHF serum on control C3A cells. Preconditioning with FHF serum did not improve cell metabolism. CONCLUSIONS:Cell preconditioning with UoE-medium increases metabolic capacity and would greatly improve BALSS efficacy.
journal_name
J Hepatoljournal_title
Journal of hepatologyauthors
Filippi C,Keatch SA,Rangar D,Nelson LJ,Hayes PC,Plevris JNdoi
10.1016/j.jhep.2004.06.012subject
Has Abstractpub_date
2004-10-01 00:00:00pages
599-605issue
4eissn
0168-8278issn
1600-0641pii
S0168827804002636journal_volume
41pub_type
杂志文章abstract：:Since the early 1990's, hepatitis B immune globulin (HBIG) has been central to the prevention of hepatitis B virus (HBV) recurrence after liver transplantation. When used in combination with oral nucleos(t)ide analogues, HBIG prevents reinfection with HBV in ⩾90% of transplant recipients. While HBIG is highly efficaci...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2011.08.026
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND/AIMS:Liver macrophages play an essential role in necro-inflammatory liver damage which leads to fibrosis and cirrhosis. The aim of the present study was to compare the mediator release and the DNA synthesis of macrophages at an early and at a later stage of liver cirrhosis induced by thioacetamide. METHODS:...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80118-3
更新日期：1997-05-01 00:00:00
abstract：:Over the last three decades acute liver failure (ALF) has been transformed from a rare and poorly understood condition with a near universally fatal outcome, to one with a well characterized phenotype and disease course. Complex critical care protocols are now applied and emergency liver transplantation (ELT) is an es...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2014.12.016
更新日期：2015-04-01 00:00:00
abstract：:In this paper, we studied the usefulness of polymerase chain reaction (PCR) in HDV-RNA detection. Using serial dilutions of serum samples of known concentrations of HDV-RNA, PCR was 10,000-times more sensitive than slot-blot hybridization. PCR was used for the detection of HDV-RNA in 33 serum samples negative to HDV-R...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(90)90225-g
更新日期：1990-11-01 00:00:00
abstract：:Pre-S1 (large S) proteins are components of the envelope of HBV. The presence of pre-S1 proteins is correlated with viral replication. To test sera of patients with HBV infection for the presence of antibodies against pre-S1 proteins (anti-pre-S1), an E. coli extract containing a pre-S fusion protein covering the grea...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(87)80005-3
更新日期：1987-02-01 00:00:00
abstract：BACKGROUND/AIMS:Intrahepatic cholestasis of pregnancy is characterized by pruritus and increased levels of serum bile acids, and is often associated with premature delivery, fetal distress, and perinatal mortality. The aims of the present study were: (i) to better define the serum bile acid profile in intrahepatic chol...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/s0168-8278(98)80207-9
更新日期：1998-01-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatitis C virus (HCV) has been recognized as a major cause of liver disease, but little is known about its diffusion at population level. To estimate the prevalence and incidence of HCV infection and to explore potential risk factors at population level, an epidemiologic study was carried out. METHOD...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80276-0
更新日期：1997-07-01 00:00:00
abstract：:The diagnostic performance of an Expert System (Jaundice) designed to discriminate between different causes of jaundice was evaluated in a test sample of 200 consecutive in-patients with serum bilirubin greater than or equal to 51 mumol/l. The average probability assigned to true diagnosis, the non-error rate and the ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(91)90069-n
更新日期：1991-11-01 00:00:00
abstract：BACKGROUND/AIMS AND METHODS:The etiology of intrahepatic cholestasis of pregnancy (JCP) is unknown. We have performed comprehensive chromatographic and mass spectrometric analyses of progesterone metabolites and bile acids in serum and urine of six patients in order to characterize changes that might be of importance f...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(97)80181-x
更新日期：1997-08-01 00:00:00
abstract：BACKGROUND & AIMS:It was recently shown that semi-annual surveillance for hepatocellular carcinoma (HCC) in cirrhotic patients provides a prognostic advantage over the annual program; however, its cost-effectiveness (CE) in the general cirrhotic population still needs to be defined. METHODS:A Markov model was built to...
journal_title：Journal of hepatology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jhep.2011.11.022
更新日期：2012-05-01 00:00:00
abstract：:As growth hormone possesses anabolic properties that are active on protein metabolism, and thus of potential benefit to patients with chronic liver disease, we determined the metabolic effects of recombinant human growth hormone on insulin-like growth factor-I (IGF-I) its specific binding proteins, and liver function....
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0168-8278(94)80228-9
更新日期：1994-11-01 00:00:00
abstract：BACKGROUND/AIMS:Hepatitis C has been associated with a decrease in quality of life and with neurological abnormalities. The aim of our study was to investigate the relationship between quality of life and cognitive function. METHODS:Quality of life, clinical variables and neuropsychological function were evaluated in ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00189-2
更新日期：2003-08-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is an accepted indication for liver transplantation (LT). Pre-LT adjuvant ablation treatments to prevent tumour progression and drop out from the waiting list have been increasingly adopted at most transplant centers. Trans-catheter arterial chemo-embolization (TACE) is frequently used, ...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2008.02.021
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND & AIMS:Given an appreciable risk of adverse-effects, current therapies for chronic hepatitis C virus (HCV) infection pose a dilemma to patients. We explored, via simulation modelling, patient-important benefits of attaining a sustained viral response (SVR). METHODS:We created the HCV Individualised Treatmen...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2014.01.020
更新日期：2014-06-01 00:00:00
abstract：BACKGROUND & AIMS:Heat shock protein (Hsp) 72 is a molecular chaperone that has broad cytoprotective functions and is upregulated in response to stress. To determine its hepatic functions, we studied its expression in human liver disorders and its biological significance in newly generated transgenic animals. METHODS:...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2018.01.003
更新日期：2018-05-01 00:00:00
abstract：BACKGROUND:The expression and the distribution of fibrillin-1 and elastin were studied in normal and pathological human liver samples. METHODS:As controls, histologically normal/subnormal liver samples (n = 24) were used. Pathological samples corresponded to seven cirrhosis and eight hepatocellular carcinomas (HCC) de...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)00048-9
更新日期：2001-04-01 00:00:00
abstract：BACKGROUND & AIMS:The incidence of cirrhosis and subsequent development of organ dysfunction (OD) requiring intensive care unit (ICU) support is rising. Historically, critically ill cirrhotics are perceived as having poor prognosis and substantial cost of care. METHODS:The aim was to prospectively analyse resource uti...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2011.12.014
更新日期：2012-05-01 00:00:00
abstract：BACKGROUND/AIMS:In this study, a detailed morphometrical analysis of the hepatic microvasculature in the different zones of hepatic parenchyma was performed in normal and cirrhotic rat liver (CCl4-induced). The aims were to detect, in CCl4-induced cirrhosis, the real presence of the "capillarization" of hepatic sinusoi...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1034/j.1600-0641.2000.033004555.x
更新日期：2000-10-01 00:00:00
abstract：:Short- and long-term effects of intraperitoneally transplanted microcarrier attached liver cells (MAL) have been studied in two experimental models of severe liver insufficiency in the rat: subtotal hepatectomy (HX) and acute liver ischemia. Intraperitoneal transplantation of MAL immediately after subtotal hepatectomy...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(89)90075-5
更新日期：1989-07-01 00:00:00
abstract：:Liver diseases affect millions of people worldwide. In most developed countries, the incidence of viral hepatitis is waning as a result of modern advances in disease prevention, diagnosis, and therapies. Expanded programmes for systematic immunisation against hepatitis B virus have also significantly brought down the ...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2019.03.004
更新日期：2019-07-01 00:00:00
abstract：BACKGROUND/AIMS:Not all alcoholic patients develop severe liver disease with fibrosis progressing to cirrhosis. It is of practical importance to determine whether some markers can predict progression of liver fibrosis. METHODS:We used a baboon model that mimics human alcoholic liver disease. Cytokeratin 7 and 19 expre...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(03)00144-2
更新日期：2003-06-01 00:00:00
abstract：BACKGROUND & AIMS:Clinical cases of viral infections possibly involving the transfusion of blood components are systematically investigated. METHODS:Serological and molecular markers of hepatitis B virus were used including HBsAg, anti-HBc, anti-HBs, HBV DNA, and viral load. Full genome sequencing and phylogenetic ana...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2011.07.034
更新日期：2012-03-01 00:00:00
abstract：BACKGROUND/AIMS:The aim of this study is to verify the efficacy and safety of peg-interferon alfa-2b in combination with ribavirin for initial treatment of HCV-associated mixed cryoglobulinemia. METHODS:Eighteen patients (7 women and 11 men) affected by mixed cryoglobulinemia were included in the study and treated wit...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jhep.2004.10.031
更新日期：2005-05-01 00:00:00
abstract：BACKGROUND/AIMS:Chronic liver disease is a well-recognised complication of cystic fibrosis. Recent reports suggest that its development is not determined by specific mutations within the cystic fibrosis gene; however, familial clustering of portal hypertension cases and inappropriate immune responses against liver memb...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/0168-8278(95)80058-1
更新日期：1995-11-01 00:00:00
abstract：:The oesophageal pH was recorded for 3 h after a test-meal in 27 healthy control subjects (group I), 40 patients with alcoholic cirrhosis (group II), and 22 patients with a normal liver and symptoms of gastro-oesophageal reflux (control refluxers). Gastro-oesophageal reflux was observed in 10 of the cirrhotic patients....
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(87)80088-0
更新日期：1987-04-01 00:00:00
abstract：BACKGROUND/AIMS:Severe alcoholic hepatitis occurs mainly in patients with cirrhosis, and has a high death rate. Corticosteroid therapy has been particularly advocated as reducing mortality in patients with severe histologic lesions. However, identification of these patients is difficult, requiring transvenous liver bio...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/s0168-8278(00)80391-8
更新日期：2000-03-01 00:00:00
abstract：:To evaluate the efficacy of recombinant interferon alpha-2b in the treatment of patients with acute post-transfusion hepatitis C, a randomized controlled trial was conducted in 33 acute post-transfusion hepatitis C patients; 16 patients received 3 million units of subcutaneously injected recombinant interferon alpha-2...
journal_title：Journal of hepatology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0168-8278(94)80246-7
更新日期：1994-11-01 00:00:00
abstract：:Reactivation of Hedgehog (Hh), a morphogenic signaling pathway that controls progenitor cell fate and tissue construction during embryogenesis occurs during many types of liver injury in adult. The net effects of activating the Hedgehog pathway include expansion of liver progenitor populations to promote liver regener...
journal_title：Journal of hepatology
pub_type： 杂志文章,评审
doi：10.1016/j.jhep.2010.10.003
更新日期：2011-02-01 00:00:00
abstract：BACKGROUND & AIMS:To determine whether amino acid mutations in the core region of hepatitis C virus (HCV) genotype 1 are associated with response to interferon (IFN) therapy and development of hepatocellular carcinoma (HCC). METHODS:We followed up 361 patients (median duration, 121 months), and IFN monotherapy was adm...
journal_title：Journal of hepatology
pub_type： 杂志文章
doi：10.1016/j.jhep.2009.10.001
更新日期：2010-01-01 00:00:00
abstract：BACKGROUND & AIMS:Evidence on the efficacy of antiviral treatment in chronic hepatitis C (CHC) patients with hepatocellular carcinoma (HCC) after curative treatment is scarce. We aimed to evaluate the efficacy and safety of pegylated interferon-alpha plus ribavirin (pegIFN/RBV) combination therapy in these patients, co...
journal_title：Journal of hepatology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jhep.2010.07.011
更新日期：2011-02-01 00:00:00
