Abstract:
:The WWOX (WW-domain containing oxidoreductase) is a candidate tumour suppressor gene spanning the same chromosome region, 16q23, as the second most common fragile site (FS), FRA16D. Deletions detected by comparative genomic hybridisation (CGH) and loss of heterozygosity at microsatellite markers on chromosome 16q are common in many human cancers including hepatocellular carcinoma (HCC). The development of human HCC is closely associated with exposure to oncogenic viruses and chemical carcinogens, agents known to frequently target common FS. We examined the status of WWOX genomic DNA, RNA and protein in 18 cell lines derived from human HCC and found recurrent alterations of the gene. Loss of DNA copy-number confined to band 16q23 was detected by CGH in several cell lines. Although homozygous deletions of the WWOX gene were not detected, WWOX mRNA expression was absent or lower in 60% of cell lines. The occurrence of aberrant WWOX reverse transcription-PCR products with deletion of exons 6-8 correlated significantly with altered WWOX expression. All of the cell lines showing mRNA downregulation had a decreased or undetectable level of WWOX protein as demonstrated by Western blotting with antibody to WWOX. Furthermore, 13 out of the 18 cell lines expressed decreased levels or no WWOX protein when compared with normal liver. These results show that WWOX gene is frequently altered in HCC and raise the possibility that this gene is implicated in hepatocarcinogenesis.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Park SW,Ludes-Meyers J,Zimonjic DB,Durkin ME,Popescu NC,Aldaz CMdoi
10.1038/sj.bjc.6602023subject
Has Abstractpub_date
2004-08-16 00:00:00pages
753-9issue
4eissn
0007-0920issn
1532-1827pii
6602023journal_volume
91pub_type
杂志文章abstract::This study was undertaken to investigate the intracellular induction of reactive oxygen species (ROS) by cis-dichlorodiammineplatinum (CDDP) and the augmentation of their cytotoxicity in bladder cancer cells (KU7) by enhancement of ROS generation by the glutathione (GSH) depletors buthionine sulphoximine (BSO) and die...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.363
更新日期:1997-01-01 00:00:00
abstract::Ordinary lipomas are cytogenetically characterized by a variety of balanced rearrangements involving chromosome segment 12q13-15, whereas well differentiated liposarcomas (WDL) show supernumerary ring and giant marker chromosomes, known to contain amplified 12q sequences. The tight correlation between the presence of ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.1090
更新日期:2000-04-01 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2000.1737
更新日期:2001-05-04 00:00:00
abstract::There is disagreement concerning the expression of thyroid peroxidase (TPO) in thyroid cancer, some studies finding qualitative as well as quantitative differences compared to normal tissue. To investigate TPO protein expression and its antigenic properties, TPO was captured from a solubilizate of thyroid microsomes b...
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pub_type: 杂志文章
doi:10.1054/bjoc.2001.2015
更新日期:2001-09-14 00:00:00
abstract::Eleven groups of workers submitted a total of 21 bronchial tumour-associated antigen preparations and 19 antisera for comparative studies. Many of the antisera proved to be polyspecific despite absorption procedures. Most of the antigen preparations contained some material reactive towards a reference antiserum to nor...
journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1981-10-01 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究
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abstract::Advances in understanding the role of vascular endothelial growth factor (VEGF) in normal physiology are giving insight into the basis of adverse effects attributed to the use of VEGF inhibitors in clinical oncology. These effects are typically downstream consequences of suppression of cellular signalling pathways imp...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6603813
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pub_type: 杂志文章
doi:10.1054/bjoc.2001.1929
更新日期:2001-08-03 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6601715
更新日期:2004-04-05 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605209
更新日期:2009-08-18 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.363
更新日期:1998-06-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.32
更新日期:2013-03-05 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/s41416-020-01084-0
更新日期:2020-12-01 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1977.198
更新日期:1977-09-01 00:00:00
abstract:BACKGROUND:Renal cell carcinoma (RCC) is highly resistant to chemotherapy because of a high apoptotic threshold. Recent evidences suggest that GSK-3beta positively regulates human pancreatic cancer and leukaemia cell survival in part through regulation of nuclear factor (NF-kappaB)-mediated expression of anti-apoptotic...
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pub_type: 杂志文章
doi:10.1038/sj.bjc.6605437
更新日期:2009-12-15 00:00:00
abstract::Magnetic resonance spectroscopy is increasingly used as a non-invasive method to investigate apoptosis. Apoptosis was induced in Jurkat T-cells by Fas mAb. (1)H magnetic resonance spectra of live cells showed an increase in methylene signal as well as methylene/methyl ratio of fatty acid side chains at 5 and 24 h foll...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600188
更新日期:2002-03-18 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2001.2185
更新日期:2001-12-14 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.1999.0938
更新日期:2000-01-01 00:00:00
abstract::DNA flow cytometry was performed on formalin fixed, paraffin embedded melanocytic naevi. DNA aneuploidy was detected in all three types of naevus but was significantly more frequent in those naevi accepted as precursors of malignancy: that is, dysplastic and congenital pigmented hairy naevi. It may be that the presenc...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1988.268
更新日期:1988-11-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1985.151
更新日期:1985-07-01 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1994.372
更新日期:1994-10-01 00:00:00
abstract:BACKGROUND:Increased survival after cancer in young age has made long-term follow-up studies of high external validity important. In this national cohort study, we explored the impact of cancer in young age on reproduction and marital status in male survivors. METHODS:Hazard ratios (HRs) and relative risks (RRs) of re...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.455
更新日期:2016-02-02 00:00:00
abstract:BACKGROUND:Patients whose colorectal cancer is treated after an emergency admission tend to have late-stage cancer and a poor prognosis. We identified risk factors for an emergency admission by linking data from the National Bowel Cancer Audit (NBCA) and the English Hospital Episode Statistics (HES), an administrative ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.300
更新日期:2014-07-29 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6606025
更新日期:2011-01-18 00:00:00
abstract::To investigate possible relationships between genetic alterations and hormonal deregulation during breast cancer development and/or progression, we examined 616 primary breast cancers for loss of heterozygosity (LOH) at chromosomal regions 16q24, 17p13.3 and 17q21, and for amplifications of the ERBB2 and c-MYC loci. A...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1995.89
更新日期:1995-03-01 00:00:00
abstract::A brief account is given of two types of rare soft tissue sarcoma of uncertain histogenesis. The danger of mistaking the epitheloid sarcoma for a benign lesion is emphasized. ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1971.58
更新日期:1971-09-01 00:00:00
abstract::Recently, we have found a high frequency of p53 gene mutations in human functional adrenal tumours. As the tumorigenesis is a multigene defect, we believe that other oncogenes may also be involved in the initiation or progression of adrenal tumours. Using the single-strand conformational polymorphism (SSCP) method, we...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1998.177
更新日期:1998-04-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/sj.bjc.6603494
更新日期:2007-02-12 00:00:00
abstract::The triple drug combination consisting of irinotecan, oxaliplatin and 5-fluorouracil (FOLFOXIRI) has demonstrated higher activity and efficacy compared to the doublet FOLFIRI. 5-Fluorouracil could be substituted in FOLFOXIRI regimen by capecitabine, an oral fluoropyrimidine with similar efficacy. Recently, a dose-find...
journal_title:British journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1038/sj.bjc.6605075
更新日期:2009-06-02 00:00:00