Genetic variation of SARS coronavirus in Beijing Hospital.

Abstract:

:To characterize genetic variation of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) transmitted in the Beijing area during the epidemic outbreak of 2003, we sequenced 29 full-length S genes of SARS-CoV from 20 hospitalized SARS patients on our unit, the Beijing 302 Hospital. Viral RNA templates for the S-gene amplification were directly extracted from raw clinical samples, including plasma, throat swab, sputum, and stool, during the course of the epidemic in the Beijing area. We used a TA-cloning assay with direct analysis of nested reverse transcription-polymerase chain reaction products in sequence. One hundred thirteen sequence variations with nine recurrent variant sites were identified in analyzed S-gene sequences compared with the BJ01 strain of SARS-CoV. Among them, eight variant sites were, we think, the first documented. Our findings demonstrate the coexistence of S-gene sequences with and without substitutions (compared to BJ01) in samples analyzed from some patients.

journal_name

Emerg Infect Dis

authors

Xu D,Zhang Z,Chu F,Li Y,Jin L,Zhang L,Gao GF,Wang FS

doi

10.3201/eid1005.030875

subject

Has Abstract

pub_date

2004-05-01 00:00:00

pages

789-94

issue

5

eissn

1080-6040

issn

1080-6059

journal_volume

10

pub_type

杂志文章
  • Clinical findings of West Nile virus infection in hospitalized patients, New York and New Jersey, 2000.

    abstract::Outbreaks of West Nile (WN) virus occurred in the New York metropolitan area in 1999 and 2000. Nineteen patients diagnosed with WN infection were hospitalized in New York and New Jersey in 2000 and were included in this review. Eleven patients had encephalitis or meningoencephalitis, and eight had meningitis alone. Ag...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid0704.010409

    authors: Weiss D,Carr D,Kellachan J,Tan C,Phillips M,Bresnitz E,Layton M,West Nile Virus Outbreak Response Working Group.

    更新日期:2001-07-01 00:00:00

  • Plasmodium falciparum Histidine-Rich Protein 2 and 3 Gene Deletions in Strains from Nigeria, Sudan, and South Sudan.

    abstract::Deletion of histidine-rich protein genes pfhrp2/3 in Plasmodium falciparum causes infections to go undetected by HRP2-based malaria rapid diagnostic tests. We analyzed P. falciparum malaria cases imported to Australia (n = 210, collected 2010-2018) for their pfhrp2/3 status. We detected gene deletions in patients from...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2702.191410

    authors: Prosser C,Gresty K,Ellis J,Meyer W,Anderson K,Lee R,Cheng Q

    更新日期:2021-02-01 00:00:00

  • Imported Monkeypox, Singapore.

    abstract::In May 2019, we investigated monkeypox in a traveler from Nigeria to Singapore. The public health response included rapid identification of contacts, use of quarantine, and postexposure smallpox vaccination. No secondary cases were identified. Countries should develop surveillance systems to detect emerging infectious...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2608.191387

    authors: Yong SEF,Ng OT,Ho ZJM,Mak TM,Marimuthu K,Vasoo S,Yeo TW,Ng YK,Cui L,Ferdous Z,Chia PY,Aw BJW,Manauis CM,Low CKK,Chan G,Peh X,Lim PL,Chow LPA,Chan M,Lee VJM,Lin RTP,Heng MKD,Leo YS

    更新日期:2020-08-01 00:00:00

  • Linezolid dependence in Staphylococcus epidermidis bloodstream isolates.

    abstract::We document linezolid dependence among 5 highly linezolid-resistant (LRSE) Staphylococcus epidermidis bloodstream isolates that grew substantially faster at 32 µg/mL linezolid presence. These isolates carried the mutations T2504A and C2534T in multiple 23S rRNA copies and 2 mutations leading to relevant amino acid sub...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1901.111527

    authors: Pournaras S,Ntokou E,Zarkotou O,Ranellou K,Themeli-Digalaki K,Stathopoulos C,Tsakris A

    更新日期:2013-01-01 00:00:00

  • Emerging Chagas disease: trophic network and cycle of transmission of Trypanosoma cruzi from palm trees in the Amazon.

    abstract::A trophic network involving molds, invertebrates, and vertebrates, ancestrally adapted to the palm tree (Attalaea phalerata) microhabitat, maintains enzootic Trypanosoma cruzi infections in the Amazonian county Paço do Lumiar, state of Maranhão, Brazil. We assessed seropositivity for T. cruzi infections in the human p...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid0701.700100

    authors: Teixeira AR,Monteiro PS,Rebelo JM,Argañaraz ER,Vieira D,Lauria-Pires L,Nascimento R,Vexenat CA,Silva AR,Ault SK,Costa JM

    更新日期:2001-01-01 00:00:00

  • Global distribution of novel rhinovirus genotype.

    abstract::Global surveillance for a novel rhinovirus genotype indicated its association with community outbreaks and pediatric respiratory disease in Africa, Asia, Australia, Europe, and North America. Molecular dating indicates that these viruses have been circulating for at least 250 years. ...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1406.080271

    authors: Briese T,Renwick N,Venter M,Jarman RG,Ghosh D,Köndgen S,Shrestha SK,Hoegh AM,Casas I,Adjogoua EV,Akoua-Koffi C,Myint KS,Williams DT,Chidlow G,van den Berg R,Calvo C,Koch O,Palacios G,Kapoor V,Villari J,Dominguez S

    更新日期:2008-06-01 00:00:00

  • Fluoroquinolone resistance in penicillin-resistant Streptococcus pneumoniae clones, Spain.

    abstract::Among 2,882 Streptococcus pneumoniae sent to the Spanish Reference Laboratory during 2002, 75 (2.6%) were ciprofloxacin-resistant. Resistance was associated with older patients (3.9% in adults and 7.2% in patients > or =65 years of age), with isolation from noninvasive sites (4.3% vs. 1.0%), and with penicillin and ma...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1010.040382

    authors: de la Campa AG,Balsalobre L,Ardanuy C,Fenoll A,Pérez-Trallero E,Liñares J,Spanish Pneumococcal Infection Study Network G03\/103.

    更新日期:2004-10-01 00:00:00

  • Lessons Learned from Emergency Response Vaccination Efforts for Cholera, Typhoid, Yellow Fever, and Ebola.

    abstract::Countries must be prepared to respond to public health threats associated with emergencies, such as natural disasters, sociopolitical conflicts, or uncontrolled disease outbreaks. Rapid vaccination of populations vulnerable to epidemic-prone vaccine-preventable diseases is a major component of emergency response. Emer...

    journal_title:Emerging infectious diseases

    pub_type: 历史文章,杂志文章

    doi:10.3201/eid2313.170550

    authors: Walldorf JA,Date KA,Sreenivasan N,Harris JB,Hyde TB

    更新日期:2017-12-01 00:00:00

  • Exposure of domestic mammals to West Nile virus during an outbreak of human encephalitis, New York City, 1999.

    abstract::We evaluated West Nile (WN) virus seroprevalence in healthy horses, dogs, and cats in New York City after an outbreak of human WN virus encephalitis in 1999. Two (3%) of 73 horses, 10 (5%) of 189 dogs, and none of 12 cats tested positive for WN virus-neutralizing antibodies. Domestic mammals should be evaluated as sen...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid0704.010424

    authors: Komar N,Panella NA,Boyce E

    更新日期:2001-07-01 00:00:00

  • Naturally Acquired Human Plasmodium cynomolgi and P. knowlesi Infections, Malaysian Borneo.

    abstract::To monitor the incidence of Plasmodium knowlesi infections and determine whether other simian malaria parasites are being transmitted to humans, we examined 1,047 blood samples from patients with malaria at Kapit Hospital in Kapit, Malaysia, during June 24, 2013-December 31, 2017. Using nested PCR assays, we found 845...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2608.200343

    authors: Raja TN,Hu TH,Kadir KA,Mohamad DSA,Rosli N,Wong LL,Hii KC,Simon Divis PC,Singh B

    更新日期:2020-08-01 00:00:00

  • Hepatitis E virus genotype 3 in humans and Swine, Bolivia.

    abstract::We determined the seroprevalence of hepatitis E virus (HEV) in persons in 2 rural communities in southeastern Bolivia and the presence of HEV in human and swine fecal samples. HEV seroprevalence was 6.3%, and HEV genotype 3 strains with high sequence homology were detected. ...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1708.100769

    authors: Dell'Amico MC,Cavallo A,Gonzales JL,Bonelli SI,Valda Y,Pieri A,Segund H,Ibañez R,Mantella A,Bartalesi F,Tolari F,Bartoloni A

    更新日期:2011-08-01 00:00:00

  • Serologic evidence of lyssavirus infection in bats, Cambodia.

    abstract::In Cambodia, 1,303 bats of 16 species were tested for lyssavirus. No lyssavirus nucleocapsid was detected in 1,283 brains tested by immunofluorescence assay. Antibodies against lyssaviruses were detected by enzyme-linked immunosorbent assay in 144 (14.7%) of 981 serum samples. Thirty of 187 serum samples contained neu...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1012.040459

    authors: Reynes JM,Molia S,Audry L,Hout S,Ngin S,Walston J,Bourhy H

    更新日期:2004-12-01 00:00:00

  • Effectiveness of seasonal influenza vaccine against pandemic (H1N1) 2009 virus, Australia, 2010.

    abstract::To estimate effectiveness of seasonal trivalent and monovalent influenza vaccines against pandemic influenza A (H1N1) 2009 virus, we conducted a test-negative case-control study in Victoria, Australia, in 2010. Patients seen for influenza-like illness by general practitioners in a sentinel surveillance network during ...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1707.101959

    authors: Fielding JE,Grant KA,Garcia K,Kelly HA

    更新日期:2011-07-01 00:00:00

  • Low Circulation of Zika Virus, Cambodia, 2007-2016.

    abstract::We describe a retrospective study on circulation of Zika virus in Cambodia during 2007-2016 among patients with dengue-like symptoms and Aedes aegypti mosquitoes. Our findings suggest that Zika virus in Cambodia belongs to the Asia genotype, is endemic, has low prevalence, and has had low-level impact on public health...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2302.161432

    authors: Duong V,Ong S,Leang R,Huy R,Ly S,Mounier U,Ou T,In S,Peng B,Ken S,Buchy P,Tarantola A,Horwood PF,Dussart P

    更新日期:2017-02-01 00:00:00

  • Population Responses during the Pandemic Phase of the Influenza A(H1N1)pdm09 Epidemic, Hong Kong, China.

    abstract::During August 2009-July 2010, we conducted 7 longitudinal telephone surveys among 503 adults in Hong Kong, China, to explore changes in their behavioral and psychological responses to the influenza A(H1N1)pdm09 virus epidemic. Trends were examined using generalized estimating equations models. Findings showed that res...

    journal_title:Emerging infectious diseases

    pub_type: 历史文章,杂志文章

    doi:10.3201/eid2305.160768

    authors: Yeung NCY,Lau JTF,Choi KC,Griffiths S

    更新日期:2017-05-01 00:00:00

  • Household responses to school closure resulting from outbreak of influenza B, North Carolina.

    abstract::School closure is a proposed strategy for reducing influenza transmission during a pandemic. Few studies have assessed how families respond to closures, or whether other interactions during closure could reduce this strategy's effect. Questionnaires were administered to 220 households (438 adults and 355 children) wit...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1407.080096

    authors: Johnson AJ,Moore ZS,Edelson PJ,Kinnane L,Davies M,Shay DK,Balish A,McCarron M,Blanton L,Finelli L,Averhoff F,Bresee J,Engel J,Fiore A

    更新日期:2008-07-01 00:00:00

  • Hospitalizations for pandemic (H1N1) 2009 among Maori and Pacific Islanders, New Zealand.

    abstract::Community transmission of influenza A pandemic (H1N1) 2009 was followed by high rates of hospital admissions in the Wellington region of New Zealand, particularly among Maori and Pacific Islanders. These findings may help health authorities anticipate the effects of pandemic (H1N1) 2009 in other communities. ...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1601.090994

    authors: Verrall A,Norton K,Rooker S,Dee S,Olsen L,Tan CE,Paull S,Allen R,Blackmore TK

    更新日期:2010-01-01 00:00:00

  • Localized Outbreaks of Epidemic Polyarthritis among Military Personnel Caused by Different Sublineages of Ross River Virus, Northeastern Australia, 2016-2017.

    abstract::Two outbreaks of epidemic polyarthritis occurred among Australian Defence Force personnel during and following short military exercises in the Shoalwater Bay Training Area, northeastern Australia, in 2016 and 2017. Ross River virus (RRV) IgM was detected in acute-phase serum samples from most patients (28/28 in 2016 a...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2510.181610

    authors: Liu W,Kizu JR,Le Grand LR,Moller CG,Carthew TL,Mitchell IR,Gubala AJ,Aaskov JG

    更新日期:2019-10-01 00:00:00

  • Endemicity of Yaws and Seroprevalence of Treponema pallidum Antibodies in Nonhuman Primates, Kenya.

    abstract::Human yaws has historically been endemic to Kenya, but current epidemiologic data are lacking. We report seroprevalence for Treponema pallidum antibodies in olive baboons (Papio anubis) and vervet monkeys (Chlorocebus pygerythrus) in Laikipia County, Kenya. Our results suggest endemicity of the yaws bacterium in monke...

    journal_title:Emerging infectious diseases

    pub_type: 信件

    doi:10.3201/eid2511.190716

    authors: Zimmerman DM,Hardgrove EH,von Fricken ME,Kamau J,Chai D,Mutura S,Kivali V,Hussein F,Ambala P,Surmat A,Maina JG,Knauf S

    更新日期:2019-11-01 00:00:00

  • Novel type of Streptococcus pneumoniae causing multidrug-resistant acute otitis media in children.

    abstract::After our recent discovery of a Streptococcus pneumoniae 19A "superbug" (Legacy strain) that is resistant to all Food and Drug Administration-approved antimicrobial drugs for treatment of acute otitis media (AOM) in children, other S. pneumoniae isolates from children with AOM were characterized by multilocus sequence...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1504.071704

    authors: Xu Q,Pichichero ME,Casey JR,Zeng M

    更新日期:2009-04-01 00:00:00

  • West Nile fever--a reemerging mosquito-borne viral disease in Europe.

    abstract::West Nile virus causes sporadic cases and outbreaks of human and equine disease in Europe (western Mediterranean and southern Russia in 1962-64, Belarus and Ukraine in the 1970s and 1980s, Romania in 1996-97, Czechland in 1997, and Italy in 1998). Environmental factors, including human activities, that enhance populat...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章,评审

    doi:10.3201/eid0505.990505

    authors: Hubálek Z,Halouzka J

    更新日期:1999-09-01 00:00:00

  • Delinquent mortgages, neglected swimming pools, and West Nile virus, California.

    abstract::Adjustable rate mortgages and the downturn in the California housing market caused a 300% increase in notices of delinquency in Bakersfield, Kern County. This led to large numbers of neglected swimming pools, which were associated with a 276% increase in the number of human West Nile virus cases during the summer of 2...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1411.080719

    authors: Reisen WK,Takahashi RM,Carroll BD,Quiring R

    更新日期:2008-11-01 00:00:00

  • Primary amebic meningoencephalitis caused by Naegleria fowleri, Karachi, Pakistan.

    abstract::We report 13 cases of Naegleria fowleri primary amebic meningoencephalitis in persons in Karachi, Pakistan, who had no history of aquatic activities. Infection likely occurred through ablution with tap water. An increase in primary amebic meningoencephalitis cases may be attributed to rising temperatures, reduced leve...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1702.100442

    authors: Shakoor S,Beg MA,Mahmood SF,Bandea R,Sriram R,Noman F,Ali F,Visvesvara GS,Zafar A

    更新日期:2011-02-01 00:00:00

  • Real-Time Surveillance in Emergencies Using the Early Warning Alert and Response Network.

    abstract::Humanitarian emergencies often result in population displacement and increase the risk for transmission of communicable diseases. To address the increased risk for outbreaks during humanitarian emergencies, the World Health Organization developed the Early Warning Alert and Response Network (EWARN) for early detection...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid2313.170446

    authors: Cordes KM,Cookson ST,Boyd AT,Hardy C,Malik MR,Mala P,El Tahir K,Everard M,Jasiem M,Husain F

    更新日期:2017-11-01 00:00:00

  • Effects of hand hygiene campaigns on incidence of laboratory-confirmed influenza and absenteeism in schoolchildren, Cairo, Egypt.

    abstract::To evaluate the effectiveness of an intensive hand hygiene campaign on reducing absenteeism caused by influenza-like illness (ILI), diarrhea, conjunctivitis, and laboratory-confirmed influenza, we conducted a randomized control trial in 60 elementary schools in Cairo, Egypt. Children in the intervention schools were r...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章,随机对照试验

    doi:10.3201/eid1704.101353

    authors: Talaat M,Afifi S,Dueger E,El-Ashry N,Marfin A,Kandeel A,Mohareb E,El-Sayed N

    更新日期:2011-04-01 00:00:00

  • Genetic divergence of Toxoplasma gondii strains associated with ocular toxoplasmosis, Brazil.

    abstract::Previous studies have shown a high prevalence of toxoplasmosis and the frequent occurrence of ocular disease in Brazil. To identify the genotypes of parasite strains associated with ocular disease, we compared 25 clinical and animal isolates of Toxoplasma gondii from Brazil to previously characterized clonal lineages ...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1206.060025

    authors: Khan A,Jordan C,Muccioli C,Vallochi AL,Rizzo LV,Belfort R Jr,Vitor RW,Silveira C,Sibley LD

    更新日期:2006-06-01 00:00:00

  • Household-level spatiotemporal patterns of incidence of cholera, Haiti, 2011.

    abstract::A cholera outbreak began in Haiti during October, 2010. Spatiotemporal patterns of household-level cholera in Ouest Department showed that the initial clusters tended to follow major roadways; subsequent clusters occurred further inland. Our data highlight transmission pathway complexities and the need for case and ho...

    journal_title:Emerging infectious diseases

    pub_type: 历史文章,杂志文章

    doi:10.3201/eid2009.131882

    authors: Blackburn JK,Diamond U,Kracalik IT,Widmer J,Brown W,Morrissey BD,Alexander KA,Curtis AJ,Ali A,Morris JG Jr

    更新日期:2014-09-01 00:00:00

  • Swine influenza virus antibodies in humans, western Europe, 2009.

    abstract::Serologic studies for swine influenza viruses (SIVs) in humans with occupational exposure to swine have been reported from the Americas but not from Europe. We compared levels of neutralizing antibodies against 3 influenza viruses--pandemic (H1N1) 2009, an avian-like enzootic subtype H1N1 SIV, and a 2007-08 seasonal s...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:10.3201/eid1703.100851

    authors: Gerloff NA,Kremer JR,Charpentier E,Sausy A,Olinger CM,Weicherding P,Schuh J,Van Reeth K,Muller CP

    更新日期:2011-03-01 00:00:00

  • Evidence-based tool for triggering school closures during influenza outbreaks, Japan.

    abstract::Guidelines available to school administrators to support school closure decisions during influenza outbreaks are usually not evidence-based. Using empirical data on absentee rates of elementary school students in Japan, we developed a simple and practical algorithm for determining the optimal timing of school closures...

    journal_title:Emerging infectious diseases

    pub_type: 杂志文章

    doi:

    authors: Sasaki A,Hoen AG,Ozonoff A,Suzuki H,Tanabe N,Seki N,Saito R,Brownstein JS

    更新日期:2009-11-01 00:00:00

  • Recovery of Cryptococcus gattii from an Infected Ventriculo-Peritoneal Shunt, Illinois, USA.

    abstract::Cryptococcus gattii is a fungal pathogen endemic in tropical and subtropical regions. Isolated cases and outbreaks have been reported in areas of North America and Europe, expanding the distribution pattern beyond warmer regions. We describe a case of ventriculo-peritoneal shunt infection by C. gattii in an immunocomp...

    journal_title:Emerging infectious diseases

    pub_type: 信件

    doi:10.3201/eid2407.171754

    authors: Moritz D,Mena Lora A,Blumer B,Harrington AT

    更新日期:2018-07-01 00:00:00