Chromatin-mediated restriction of nuclear factor 1/CTF binding in a repressed and hormone-activated promoter in vivo.

abstract：:Keratins K5 and K14 form the extensive intermediate filament network of mitotically active basal cells in all stratified epithelia. We have explored the regulatory mechanisms governing cell-type-specific and differentiation stage-specific expression of the human K5 gene in transiently transfected keratinocytes in vitr...

journal_title：Molecular and cellular biology

authors： Byrne C,Fuchs E

更新日期：1993-06-01 00:00:00

abstract：:Eukaryotic splicing factors belonging to the SR family are essential splicing factors consisting of an N-terminal RNA-binding region and a C-terminal RS domain. They are believed to be involved in alternative splicing of numerous transcripts because their expression levels can influence splice site selection. We have ...

journal_title：Molecular and cellular biology

authors： Jumaa H,Guénet JL,Nielsen PJ

更新日期：1997-06-01 00:00:00

abstract：:The message for the zinc finger gene Rex-1 (Zfp-42) is expressed in undifferentiated murine F9 teratocarcinoma cells and embryonic stem cells. Expression of Rex-1 is reduced at the transcriptional level when F9 cells are induced by the addition of retinoic acid (RA) to differentiate. We have isolated genomic DNA for t...

journal_title：Molecular and cellular biology

authors： Hosler BA,Rogers MB,Kozak CA,Gudas LJ

更新日期：1993-05-01 00:00:00

abstract：:hsa-miR-33a and hsa-miR-33b, intronic microRNAs (miRNAs) located within the sterol regulatory element-binding protein 2 and 1 genes (Srebp-2 and -1), respectively, have recently been shown to regulate lipid homeostasis in concert with their host genes. Although the functional role of miR-33a and -b has been highly inv...

journal_title：Molecular and cellular biology

authors： Goedeke L,Vales-Lara FM,Fenstermaker M,Cirera-Salinas D,Chamorro-Jorganes A,Ramírez CM,Mattison JA,de Cabo R,Suárez Y,Fernández-Hernando C

更新日期：2013-06-01 00:00:00

abstract：:Nuclear extracts from chicken erythroid cells selectively stimulate transcription of the chicken histone H5 gene (and not of other chicken histone genes) after coinjection into frog oocytes. This effect is shown to involve an enhancerlike activity, and a region of the H5 gene sufficient to mediate trans-activation is ...

journal_title：Molecular and cellular biology

authors： Wigley PL,Wells JR

更新日期：1987-10-01 00:00:00

abstract：:The protein tyrosine kinase c-Src is regulated by two intramolecular interactions. The repressed state is achieved through the interaction of the Src homology 2 (SH2) domain with the phosphorylated C-terminal tail and the association of the SH3 domain with a polyproline type II helix formed by the linker region betwee...

journal_title：Molecular and cellular biology

authors： Adolph D,Flach N,Mueller K,Ostareck DH,Ostareck-Lederer A

更新日期：2007-03-01 00:00:00

abstract：:Using an expression cloning assay, we have isolated a novel cDNA, referred to as rsp-1, which suppresses the v-Ras-transformed phenotype. When introduced into NIH 3T3 fibroblasts under the control of a metallothionein promoter, rsp-1 confers resistance to v-Ras, but not to v-Mos or v-Src, and inhibits growth of the ce...

journal_title：Molecular and cellular biology

authors： Cutler ML,Bassin RH,Zanoni L,Talbot N

更新日期：1992-09-01 00:00:00

abstract：:The Src homology 2-containing phosphotyrosine phosphatase (SHP2) is primarily a positive effector of receptor tyrosine kinase signaling. However, the molecular mechanism by which SHP2 effects its biological function is unknown. In this report, we provide evidence that defines the molecular mechanism and site of action...

journal_title：Molecular and cellular biology

authors： Agazie YM,Hayman MJ

更新日期：2003-11-01 00:00:00

abstract：:Centrosomes ensure accurate chromosome segregation by directing spindle bipolarity. Loss of centrosome regulation results in centrosome amplification, multipolar mitosis and aneuploidy. Since centrosome amplification is common in premalignant lesions and breast tumors, it is proposed to play a central role in breast t...

journal_title：Molecular and cellular biology

authors： Lee MY,Moreno CS,Saavedra HI

更新日期：2014-07-01 00:00:00

abstract：:There are five members of the RFX family of transcription factors in mammals. While RFX5 plays a well-defined role in the immune system, the functions of RFX1 to RFX4 remain largely unknown. We have generated mice with a deletion of the Rfx3 gene. RFX3-deficient mice exhibit frequent left-right (LR) asymmetry defects ...

journal_title：Molecular and cellular biology

authors： Bonnafe E,Touka M,AitLounis A,Baas D,Barras E,Ucla C,Moreau A,Flamant F,Dubruille R,Couble P,Collignon J,Durand B,Reith W

更新日期：2004-05-01 00:00:00

abstract：:Metallothioneins (MTs) are short, cysteine-rich proteins for heavy metal homeostasis and detoxification; they bind a variety of heavy metals and also act as radical scavengers. Transcription of mammalian MT genes is activated by heavy metal load via the metal-responsive transcription factor 1 (MTF-1), an essential zin...

journal_title：Molecular and cellular biology

authors： Zhang B,Egli D,Georgiev O,Schaffner W

更新日期：2001-07-01 00:00:00

abstract：:TDP-43, or TAR DNA-binding protein 43, is a pathological marker of a spectrum of neurodegenerative disorders, including amyotrophic lateral sclerosis and frontotemporal lobar degeneration with ubiquitin-positive inclusions. TDP-43 is an RNA/DNA-binding protein implicated in transcriptional and posttranscriptional regu...

journal_title：Molecular and cellular biology

authors： Dewey CM,Cenik B,Sephton CF,Dries DR,Mayer P 3rd,Good SK,Johnson BA,Herz J,Yu G

更新日期：2011-03-01 00:00:00

abstract：:To examine a role for focal adhesion kinase (FAK) in cardiac morphogenesis, we generated a line of mice with a conditional deletion of FAK in nkx2-5-expressing cells (herein termed FAKnk mice). FAKnk mice died shortly after birth, likely resulting from a profound subaortic ventricular septal defect and associated mala...

journal_title：Molecular and cellular biology

authors： Hakim ZS,DiMichele LA,Doherty JT,Homeister JW,Beggs HE,Reichardt LF,Schwartz RJ,Brackhan J,Smithies O,Mack CP,Taylor JM

更新日期：2007-08-01 00:00:00

abstract：:Endonuclease G (endo G) is one of the most abundant nucleases in eukaryotic cells. It is encoded in the nucleus and imported to the mitochondrial intermembrane space. This nuclease is active on single- and double-stranded DNA. We genetically disrupted the endo G gene in mice without disturbing a conserved, overlapping...

journal_title：Molecular and cellular biology

authors： Irvine RA,Adachi N,Shibata DK,Cassell GD,Yu K,Karanjawala ZE,Hsieh CL,Lieber MR

更新日期：2005-01-01 00:00:00

abstract：:Transcription initiation factor TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. To further understand the role of the 85-kDa TFIID subunit (p85), we have cloned the corresponding cDNA with a probe based on an amino acid sequence of t...

journal_title：Molecular and cellular biology

authors： Kokubo T,Gong DW,Yamashita S,Takada R,Roeder RG,Horikoshi M,Nakatani Y

更新日期：1993-12-01 00:00:00

abstract：:The transcriptional corepressor mSin3 is associated with histone deacetylases (HDACs) and is utilized by many DNA-binding transcriptional repressors. We have cloned and characterized a novel mSin3A-binding protein, SAP25. SAP25 binds to the PAH1 domain of mSin3A, associates with the mSin3A-HDAC complex in vivo, and re...

journal_title：Molecular and cellular biology

authors： Shiio Y,Rose DW,Aur R,Donohoe S,Aebersold R,Eisenman RN

更新日期：2006-02-01 00:00:00

abstract：:CDC37 encodes a 50-kDa protein that targets intrinsically unstable oncoprotein kinases including Cdk4, Raf-1, and v-src to the molecular chaperone Hsp90, an interaction that is thought to be important for the establishment of signaling pathways. CDC37 is required for proliferation in budding yeast and is coexpressed w...

journal_title：Molecular and cellular biology

authors： Stepanova L,Finegold M,DeMayo F,Schmidt EV,Harper JW

更新日期：2000-06-01 00:00:00

abstract：:As part of an effort to characterize the spatial and functional relationships among genetic elements within the amplified dihydrofolate reductase (DHFR) domain in Chinese hamster cells, we have used a variation of the differential hybridization approach to identify cDNA clones whose genes are coamplified with DHFR in ...

journal_title：Molecular and cellular biology

authors： Foreman PK,Hamlin JL

更新日期：1989-03-01 00:00:00

abstract：:The kidney's vital filtration function depends on the structural integrity of the glomerulus, the proximal portion of the nephron. Within the glomerulus, the architecturally complex podocyte forms the final cellular barrier to filtration. Injury to the podocyte results in a morphological change called foot process eff...

journal_title：Molecular and cellular biology

authors： Yu H,Suleiman H,Kim AH,Miner JH,Dani A,Shaw AS,Akilesh S

更新日期：2013-12-01 00:00:00

abstract：:The gene encoding the 180-kDa DNA strand transfer protein beta from the yeast Saccharomyces cerevisiae was identified and sequenced. This gene, DST2 (DNA strand transferase 2), was located on chromosome VII. dst2 gene disruption mutants exhibited temperature-sensitive sporulation and a 50% longer generation time durin...

journal_title：Molecular and cellular biology

authors： Dykstra CC,Kitada K,Clark AB,Hamatake RK,Sugino A

更新日期：1991-05-01 00:00:00

abstract：:Transcriptional insulators are cis regulatory elements that organize chromatin into independently regulated domains. At the imprinted murine Igf2/H19 locus, the H19-ICR insulator prevents the activation of the Igf2 promoter on the maternal allele by enhancers that activate H19 on the same chromosome. Given the well-de...

journal_title：Molecular and cellular biology

authors： Singh V,Srivastava M

更新日期：2008-06-01 00:00:00

abstract：:In previous studies, we detected a major, unidentified Egr response element (ERE) binding complex in brain extracts. We now report that this complex contains a truncated isoform of Egr3 generated by use of an alternate translation start site at methionine 106. Furthermore, the ERE binding complex previously thought to...

journal_title：Molecular and cellular biology

authors： O'Donovan KJ,Baraban JM

更新日期：1999-07-01 00:00:00

abstract：:We have investigated the mechanism underlying repression of calcitonin/calcitonin gene-related peptide (CT/CGRP) gene expression by retinoic acid. Retinoic acid treatment of the CA77 thyroid C-cell line decreased CT/CGRP promoter activity two- to threefold, which correlates well with the decrease in calcitonin and CGR...

journal_title：Molecular and cellular biology

authors： Lanigan TM,Tverberg LA,Russo AF

更新日期：1993-10-01 00:00:00

abstract：:The nuclear receptor transcription factor, nor-1, is expressed during mammalian development predominantly in the nervous system and is induced in a cell-specific manner in nonneuronal cells in response to a variety of extracellular stimuli. To elucidate the essential developmental functions of this transcription facto...

journal_title：Molecular and cellular biology

authors： Pönniö T,Conneely OM

更新日期：2004-10-01 00:00:00

abstract：:TFIID is a multiprotein complex consisting of the TATA box binding protein and multiple tightly associated proteins (TAFIIs) that are required for transcription by selected activators. We previously reported cloning and partial characterization of human TAFII130 (hTAFII130). The central domain of hTAFII130 contains fo...

journal_title：Molecular and cellular biology

authors： Saluja D,Vassallo MF,Tanese N

更新日期：1998-10-01 00:00:00

abstract：:Previous studies have shown that expansion-prone repeats form structures that inhibit human flap endonuclease (FEN-1). We report here that faulty processing by FEN-1 initiates repeat instability in mammalian cells. Disease-length CAG tracts in Huntington's disease mice heterozygous for FEN-1 display a tendency toward ...

journal_title：Molecular and cellular biology

authors： Spiro C,McMurray CT

更新日期：2003-09-01 00:00:00

abstract：:A genomic DNA fragment containing the Raf-1 promoter region was isolated by using a cDNA extension clone. Nucleotide sequencing of genomic DNA clones, primer extension, and S1 nuclease assays have been used to identify the 5' ends of Raf-1 RNAs. Consistent with its ubiquitous expression, the Raf-1 promoter region had ...

journal_title：Molecular and cellular biology

authors： Beck TW,Brennscheidt U,Sithanandam G,Cleveland J,Rapp UR

更新日期：1990-07-01 00:00:00

abstract：:Spinal muscular atrophy (SMA) is an inherited motor neuron disease caused by homozygous loss of the Survival Motor Neuron 1 (SMN1) gene. In the absence of SMN1, inefficient inclusion of exon 7 in transcripts from the nearly identical SMN2 gene results in ubiquitous SMN decrease but selective motor neuron degeneration....

journal_title：Molecular and cellular biology

authors： Ruggiu M,McGovern VL,Lotti F,Saieva L,Li DK,Kariya S,Monani UR,Burghes AH,Pellizzoni L

更新日期：2012-01-01 00:00:00

abstract：:Protein kinase C theta (PKC theta) is unique among PKC isozymes in its translocation to the center of the immune synapse in T cells and its unique downstream signaling. Here we show that the hematopoietic protein tyrosine phosphatase (HePTP) also accumulates in the immune synapse in a PKC theta-dependent manner upon a...

journal_title：Molecular and cellular biology

authors： Nika K,Charvet C,Williams S,Tautz L,Bruckner S,Rahmouni S,Bottini N,Schoenberger SP,Baier G,Altman A,Mustelin T

更新日期：2006-03-01 00:00:00

abstract：:In eukaryotic cells, cohesion between sister chromatids allows chromosomes to biorient on the metaphase plate and holds them together until they separate into daughter cells during mitosis. Cohesion is mediated by the cohesin protein complex. Although the association of this complex with particular regions of the geno...

journal_title：Molecular and cellular biology

authors： Bausch C,Noone S,Henry JM,Gaudenz K,Sanderson B,Seidel C,Gerton JL

更新日期：2007-12-01 00:00:00