Abstract:
:Enkephalins are pentapeptides found in the human nervous system, where they are involved in the relief of pain. The interaction of these neuropeptides with the nerve cell membranes would be a key-step in the receptor binding. We have used both Fourier-transform infrared and solid-state NMR spectroscopies to shed light on the interactions responsible for the association of enkephalins with negatively charged membranes. More specifically, we have investigated the interaction of methionine-enkephalin (Menk) with DMPG and DMPS vesicles. Our results suggest that Menk interacts electrostatically with both model membranes via its terminal NH3+ group. However, the peptide induced the formation of elongated DMPG vesicles in the magnetic field. On the other hand, the association of Menk with DMPS bilayers was concentration-dependent and disrupted the membrane at high peptide concentrations. The different effect of methionine-enkephalin with the two types of anionic membranes is most likely related to the different fluidity of these systems.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Marcotte I,Ouellet M,Auger Mdoi
10.1016/j.chemphyslip.2003.10.012subject
Has Abstractpub_date
2004-02-01 00:00:00pages
175-87issue
2eissn
0009-3084issn
1873-2941pii
S0009308403001658journal_volume
127pub_type
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journal_title:Chemistry and physics of lipids
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