The role of the ganglioside lipid moiety in the process of ganglioside-cell interactions.


:The role of the ceramide moiety of gangliosides, together with the deriving aggregative properties of ganglioside in solution, in the process of ganglioside-cell interactions was studied. The natural GM1(stearoyl) and the synthetic GM1(acetyl), containing the stearoyl and acetyl groups as the acyl moiety, respectively, were used in binding experiments to rat cerebellar granule cells. Regardless of the cell culture conditions, such as the presence of absence of fetal calf serum, the association of GM1(acetyl) to the cells was much greater than that of GM1(stearoyl). GM1(acetyl) was present in the incubation medium as monomers. After incubation, a large part of the total GM1(acetyl) associated to cells, 76-93% depending on the experimental conditions, was removed by washing with protein solutions. The remaining associated ganglioside was not removed by repeating washing with protein solutions or trypsin treatments and was considered as a component of the membrane. The cell association of GM1(stearoyl), present in solution as monomers as well as micelles, could be classified as serum-labile, trypsin-labile and trypsin-stable. The trypsin-stable form of association, corresponding to the molecules stably inserted into the membrane, was proportionally higher, the proportions varying with increasing incubation time and decreasing ganglioside concentration. This form of association was particularly high when incubation was performed in the presence of fetal calf serum. Incubation experiments performed with a mixture of GM1(stearoyl) and GM1(acetyl) in a molar ratio which allowed their presence in the medium as monomers as well as mixed micelles, led to a ganglioside association suggesting that besides the aggregative properties of the molecule other ganglioside properties are involved in the ganglioside-cell interaction process.


Chem Phys Lipids


Bassi R,Sonnino S




Has Abstract


1992-07-01 00:00:00














  • Antioxidative and prooxidative effects of coumarin derivatives on free radical initiated and photosensitized peroxidation of human low-density lipoprotein.

    abstract::The antioxidative and/or prooxidative activity of 4-methylcoumanrin (MC), 7-hydroxy-4-methylcoumarin (HMC) and 7,8-dihydroxy-4-methylcoumarin (DHMC), respectively, in the peroxidation of human low-density lipoprotein (LDL) has been studied. The peroxidation was initiated either thermally by water-soluble initiator 2,2...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Liu ZQ,Yu W,Liu ZL

    更新日期:1999-12-01 00:00:00

  • The total synthesis of 2-O-arachidonoyl-1-O-stearoyl-sn-glycero-3-phosphocholine-1,3,1'-(13)C3 and -2,1'-(13)C2 by a novel chemoenzymatic method.

    abstract::2-O-Arachidonoyl-1-O-stearoyl-sn-glycero-3-phosphocholine was synthesized with carbon-13 enrichment of the three glycerol carbons and the carbonyl of the stearoyl group. Phospholipase A(2) was utilized to give optically pure lyso-PC, and only 3% acyl migration occurred during reacylation with arachidonic acid anhydrid...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Duclos RI Jr

    更新日期:2010-01-01 00:00:00

  • Isothermal lipid phase transitions.

    abstract::In liotropic lipid systems phase transitions can be induced isothermally by changing the solvent concentration or composition; alternatively, lipid composition can be modified by (bio)chemical means. The probability for isothermal phase transitions increases with the decreasing transition entropy; it is proportional t...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Cevc G

    更新日期:1991-03-01 00:00:00

  • Inhibition of pain responses by activation of CB(2) cannabinoid receptors.

    abstract::Cannabinoid receptor agonists diminish responses to painful stimuli. Extensive evidence demonstrates that CB(1) cannabinoid receptor activation inhibits pain responses. Recently, the synthesis of CB(2) cannabinoid receptor-selective agonists has allowed testing whether CB(2) receptor activation inhibits pain. CB(2) re...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Malan TP Jr,Ibrahim MM,Vanderah TW,Makriyannis A,Porreca F

    更新日期:2002-12-31 00:00:00

  • Evidence for the presence of 7-hydroperoxycholest-5-en-3 beta-ol in oxidized human LDL.

    abstract::Low density lipoprotein (LDL) cholesterol is known to be oxidized both in vitro and in vivo giving rise to oxygenated sterols. Conflicting results, however, have been reported concerning both the nature and the relative concentrations of these compounds in oxidized human LDL. We examined the extracts obtained from Cu(...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Malavasi B,Rasetti MF,Roma P,Fogliatto R,Allevi P,Catapano AL,Galli G

    更新日期:1992-10-01 00:00:00

  • The relationship between the structure of the headgroup of sphingolipids and their ability to form complex high axial ratio microstructures.

    abstract::Ceramides with chemically modified polar headgroups were prepared and examined for their ability to form complex high axial ratio microstructures (CHARMS), potential drug delivery vehicles. In general, if the modified ceramide had either a hydrogen bond donor or acceptor at C-1 and C-3, including hydrophobic or hydrop...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Goldstein AS,Gelb MH,Yager P

    更新日期:2001-01-01 00:00:00

  • Role of Rutin Nanoemulsion in Ameliorating Oxidative Stress: Pharmacokinetic and Pharmacodynamics Studies.

    abstract::Parkinson's disease is caused due to free radical generation in dopamine neurons leading to oxidative stress induced damage. The aim of this work was to ameliorate the free radical induced oxidative stress in rats by using TPGS (Tocopheryl polyethylene glycol 1000 succinate) loaded rutin nanoemulsion after oral admini...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Sharma S,Rabbani SA,Narang JK,Hyder Pottoo F,Ali J,Kumar S,Baboota S

    更新日期:2020-05-01 00:00:00

  • Structural polymorphism of hydrated ether-linked dimyristyl maltoside and melibioside.

    abstract::The structural polymorphism of two selected disaccharide glycolipids with a maltose (DMMA) and a melibiose (DMME) carbohydrate headgroup linked to dimyristyl alkyl chains were investigated by FTIR-spectroscopy, differential scanning calorimetry (DSC), small-angle X-ray scattering (SAXS) and film-balance measurements. ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Garidel P,Howe J,Milkereit G,Rössle M,Linser S,Sven Gerber,Willumeit R,Gutsmann T,Vill V,Brandenburg K

    更新日期:2008-01-01 00:00:00

  • Cationic lipids for gene delivery: many players, one goal.

    abstract::Lipid-based carriers represent the most widely used alternative to viral vectors for gene expression and gene silencing purposes. This class of non-viral vectors is particularly attractive for their ease of synthesis and chemical modifications to endow them with desirable properties. Despite combinatorial approaches h...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Ponti F,Campolungo M,Melchiori C,Bono N,Candiani G

    更新日期:2021-01-08 00:00:00

  • Luliconazole loaded lyotropic liquid crystalline nanoparticles for topical delivery: QbD driven optimization, in-vitro characterization and dermatokinetic assessment.

    abstract::Fungal infections are an important cause of morbidity and pose a serious health concern especially in immunocompromised patients. Luliconazole (LUL) is a topical imidazole antifungal drug with a broad spectrum of activity. To overcome the limitations of conventional dosage forms, LUL loaded lyotropic liquid crystallin...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Mahmood A,Rapalli VK,Waghule T,Gorantla S,Singhvi G

    更新日期:2021-01-01 00:00:00

  • Electrostatics of carboxylated anionic vesicles for improving entrapment capacity.

    abstract::Electrostatic interaction is an important secondary force affecting the structure, stability, and function of lipid vesicles (liposomes). For this study, a negatively charged lipid with carboxylic acid was mixed with phospholipid to produce anionic vesicles. The electrostatics of the carboxylated anionic vesicle (ca. ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Sou K

    更新日期:2011-03-01 00:00:00

  • Oligoarginine vectors for intracellular delivery: role of arginine side-chain orientation in chain length-dependent destabilization of lipid membranes.

    abstract::Arginine-rich peptides receive increased attention due to their capacity to cross different types of membranes and to transport cargo molecules inside cells. Even though peptide-induced destabilization has been investigated extensively, little is known about the peptide side-chain and backbone orientation with respect...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Bouchet AM,Lairion F,Ruysschaert JM,Lensink MF

    更新日期:2012-01-01 00:00:00

  • Lipid nanocarriers for microRNA delivery.

    abstract::Non-coding RNAs (ncRNAs) like microRNAs (miRNAs) or small interference RNAs (siRNAs) with their power to selectively silence any gene of interest enable the targeting of so far 'undruggable' proteins and diseases. Such RNA molecules have gained much attention from biotech and pharmaceutical companies, which led to the...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Scheideler M,Vidakovic I,Prassl R

    更新日期:2020-01-01 00:00:00

  • Protein-lipid interactions and surface activity in the pulmonary surfactant system.

    abstract::Pulmonary surfactant is a lipid-protein complex, synthesized and secreted by the respiratory epithelium of lungs to the alveolar spaces, whose main function is to reduce the surface tension at the air-liquid interface to minimize the work of breathing. The activity of surfactant at the alveoli involves three main proc...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Serrano AG,Pérez-Gil J

    更新日期:2006-06-01 00:00:00

  • Sphingomyelin ability to act as chiral selector using nanodisc electrokinetic chromatography.

    abstract::Understanding how stereochemistry affects interactions with cell membranes is important for effective drug development. Chirality has been shown to greatly effect pharmaceutical distribution and metabolism within the cell. However it has been thought that interactions with, and passive diffusion through, the membrane ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Penny WM,Palmer CP

    更新日期:2018-08-01 00:00:00

  • Inhibitors of sterol synthesis. Metabolism of [2,4-3H]5 alpha-cholest-8(14)-en-3 beta-ol-15-one after oral administration to a nonhuman primate.

    abstract::5 alpha-Cholest-8(14)-en-3 beta-ol-15-one is a potent inhibitor of cholesterol biosynthesis which has significant hypocholesterolemic activity upon oral administration to rodents and nonhuman primates. In the present study the metabolism of the 15-ketosterol has been investigated after the oral administration of a mix...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Pajewski TN,Brabson JS,Kisic A,Wang KS,Hylarides MD,Jackson EM,Schroepfer GJ Jr

    更新日期:1989-03-01 00:00:00

  • X-ray diffraction studies of lipid phase transitions in cholesterol-rich membranes at sub-zero temperatures.

    abstract::In X-ray diffraction studies of hydrated (greater than 60%) cholesterol/dioleoylphosphatidylcholine mixtures the lipid packing band showed an abrupt transition from liquid crystal-type to gel-type position and definition at a temperature which decreased progressively to almost -50 degrees C as the proportion of choles...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Finean JB,Hutchinson AL

    更新日期:1988-01-01 00:00:00

  • Mass spectrometric evidence for the anomalous chemical behavior of 11-dehydrothromboxane B2.

    abstract::When we subjected 11-dehydrothromboxane B2 (11-DTXB2), a metabolite of arachidonic acid, to standard chemical derivatization procedures we obtained a mixture of several products. Separation of the components was carried out by gas chromatography and their identification was accomplished through the study of their mass...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Ferretti A,Flanagan VP

    更新日期:1995-08-01 00:00:00

  • Digestion and absorption of a sulphoxide analogue of triacylglycerol in the rat.

    abstract::The stereochemistry of fat digestion and absorption was studied by feeding a triacylglycerol analogue to rats with a thoracic duct cannula. The analogue, rac-1,2-dioleoyl-3-S-tetradecyl-3-thioglycerol-S-oxide was chosen since its enantiomers exhibited high rotation in optical rotary dispersion (ORD) and circular dichr...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Akesson B,Michelsen P

    更新日期:1981-12-01 00:00:00

  • Lipid polymorphism and the roles of lipids in membranes.

    abstract::The reasons for lipid diversity in membranes are not understood. Here we review evidence supporting the proposal that factors related to the polymorphic capabilities of lipids provide a rationale for lipid diversity. In particular, the ability of lipids to adopt different polymorphic phases appears to be related to a ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Cullis PR,Hope MJ,Tilcock CP

    更新日期:1986-06-01 00:00:00

  • Synthesis and characterization of long-chain 1,2-dioxo compounds.

    abstract::A series of long-chain methyl esters with vicinal oxo groups (1,2-diones; 1,2-diketones) were synthesized by potassium permanganate-based oxidation of methyl esters of mono-unsaturated fatty acids. The presence of two additional carbonyl groups may facilitate the synthesis of other derivatives. The starting materials ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Knothe G

    更新日期:2002-05-01 00:00:00

  • Voltage-dependent behaviour of dolichyl phosphate-phosphatidylcholine bilayer lipid membranes.

    abstract::The current-voltage steady-state characteristics, cyclic voltammograms and capacitance-voltage steady-state relationships of bilayer lipid membranes made from dioleoylphosphatidylcholine or its mixtures with dolichyl-12 phosphate have been studied. Sustained fluctuations of the capacitance of dolichyl phosphate modifi...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Janas T,Kuczera J,Chojnacki T

    更新日期:1990-01-01 00:00:00

  • Combined force spectroscopy, AFM and calorimetric studies to reveal the nanostructural organization of biomimetic membranes.

    abstract::In this work we studied a binary lipid matrix of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (POPG), a composition that mimics the inner membrane of Escherichia coli. More specifically, liposomes with varying fractions of POPG were analys...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Suárez-Germà C,Morros A,Montero MT,Hernández-Borrell J,Domènech Ò

    更新日期:2014-10-01 00:00:00

  • Dynamical dimer structure and liquid structure of fatty acids in their binary liquid mixture: dodecanoic and 3-phenylpropionic acids system.

    abstract::Dimer structure and liquid structure of fatty acids in the binary liquid mixture of dodecanoic (LA) and 3-phenylpropionic acids (PPA) were studied through the measurements of DSC, self-diffusion coefficient (D), density, viscosity, 13C NMR spin-lattice relaxation time, small-angle X-ray scattering (SAXS), and small-an...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Iwahashi M,Takebayashi S,Umehara A,Kasahara Y,Minami H,Matsuzawa H,Inoue T,Takahashi H

    更新日期:2004-05-01 00:00:00

  • Differential scanning calorimetry studies of some analogs for the lipid component of biological membranes.

    abstract::The melting behavior of members of newly synthesized series of rac-1,2-diglycerides with substituted phenyl groups or a benzyl group on the 3-position was investigated with differential scanning calorimetry (DSC). Solution crystallized samples had single melting temperatures, higher than those of the quenched or annea...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Joseph JT,Hybl A,Flynn JH

    更新日期:1978-10-01 00:00:00

  • New 20-hydroxycholesterol-like compounds with fluorescent NBD or alkyne labels: Synthesis, in silico interactions with proteins and uptake by yeast cells.

    abstract::20-hydroxycholesterol is a signaling oxysterol with immunomodulating functions and, thus, structural analogues with reporter capabilities could be useful for studying and modulating the cellular processes concerned. We have synthesized three new 20-hydroxycholesterol-like pregn-5-en-3β-ol derivatives with fluorescent ...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Faletrov YV,Efimova VS,Horetski MS,Tugaeva KV,Frolova NS,Lin Q,Isaeva LV,Rubtsov MA,Sluchanko NN,Novikova LA,Shkumatov VM

    更新日期:2020-03-01 00:00:00

  • A sensitive cell-based method to screen for selective inhibitors of SMS1 or SMS2 using HPLC and a fluorescent substrate.

    abstract::Recent studies have revealed that sphingomyelin (SM) is involved in metabolic syndrome and is a new target of an anti-metabolic syndrome drug. Deficiencies in the enzyme SM synthase 1 (SMS1) result in severe abnormalities, whereas deficiencies in SMS2 do not. SMS1 and SMS2 synthesize SM under similar conditions, so th...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Zama K,Mitsutake S,Watanabe K,Okazaki T,Igarashi Y

    更新日期:2012-10-01 00:00:00

  • Changes induced in bovine serum albumin following interactions with the lipid peroxidation product E-2-octenal.

    abstract::Bovine serum albumin (BSA) undergoes a number of deteriorative changes when exposed to E-2-octenal. Reaction of BSA with E-2-octenal produced fluorescent BSA with an excitation maximum at 350 nm and emission maximum at 440 nm and promoted polymerization. Amino acid analysis of the modified BSA showed that the E-2-octe...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Alaiz M,Barragán S

    更新日期:1995-08-25 00:00:00

  • Synergistic activation of a family of phosphoinositide 3-kinase via G-protein coupled and tyrosine kinase-related receptors.

    abstract::Phosphoinositide 3-kinase (PI 3-kinase) is a key signaling enzyme implicated in a variety of receptor-stimulated cell responses. Stimulation of receptors possessing (or coupling to) protein-tyrosine kinase activates heterodimeric PI 3-kinases, which consist of an 85-kDa regulatory subunit (p85) containing Src-homology...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章,评审


    authors: Katada T,Kurosu H,Okada T,Suzuki T,Tsujimoto N,Takasuga S,Kontani K,Hazeki O,Ui M

    更新日期:1999-04-01 00:00:00

  • Synthesis of the 3-sulfates of S-acyl glutathione conjugated bile acids and their biotransformation by a rat liver cytosolic fraction.

    abstract::The 3-sulfates of the S-acyl glutathione (GSH) conjugates of five natural bile acids (cholic, chenodeoxycholic, deoxycholic, ursodeoxycholic, and lithocholic) were synthesized as reference standards in order to investigate their possible formation by a rat liver cytosolic fraction. Their structures were confirmed by p...

    journal_title:Chemistry and physics of lipids

    pub_type: 杂志文章


    authors: Mitamura K,Hori N,Mino S,Iida T,Hofmann AF,Ikegawa S

    更新日期:2012-04-01 00:00:00