Abstract:
:The role of the ceramide moiety of gangliosides, together with the deriving aggregative properties of ganglioside in solution, in the process of ganglioside-cell interactions was studied. The natural GM1(stearoyl) and the synthetic GM1(acetyl), containing the stearoyl and acetyl groups as the acyl moiety, respectively, were used in binding experiments to rat cerebellar granule cells. Regardless of the cell culture conditions, such as the presence of absence of fetal calf serum, the association of GM1(acetyl) to the cells was much greater than that of GM1(stearoyl). GM1(acetyl) was present in the incubation medium as monomers. After incubation, a large part of the total GM1(acetyl) associated to cells, 76-93% depending on the experimental conditions, was removed by washing with protein solutions. The remaining associated ganglioside was not removed by repeating washing with protein solutions or trypsin treatments and was considered as a component of the membrane. The cell association of GM1(stearoyl), present in solution as monomers as well as micelles, could be classified as serum-labile, trypsin-labile and trypsin-stable. The trypsin-stable form of association, corresponding to the molecules stably inserted into the membrane, was proportionally higher, the proportions varying with increasing incubation time and decreasing ganglioside concentration. This form of association was particularly high when incubation was performed in the presence of fetal calf serum. Incubation experiments performed with a mixture of GM1(stearoyl) and GM1(acetyl) in a molar ratio which allowed their presence in the medium as monomers as well as mixed micelles, led to a ganglioside association suggesting that besides the aggregative properties of the molecule other ganglioside properties are involved in the ganglioside-cell interaction process.
journal_name
Chem Phys Lipidsjournal_title
Chemistry and physics of lipidsauthors
Bassi R,Sonnino Sdoi
10.1016/0009-3084(92)90048-tsubject
Has Abstractpub_date
1992-07-01 00:00:00pages
1-9issue
1eissn
0009-3084issn
1873-2941pii
0009-3084(92)90048-Tjournal_volume
62pub_type
杂志文章abstract::A polymorphic variation of sialic acid species of sialosyllactosylceramide was found in dog erythrocytes. The analysis of the glycolipids in the erythrocytes of the individual dogs in a family of a Japanese breed of dog, Shiba-Inu, showed that the expression of sialosyllactosylceramide containing N-glycolylneuraminic ...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/0009-3084(86)90044-7
更新日期:1986-12-15 00:00:00
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更新日期:2016-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
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更新日期:2013-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2007.08.001
更新日期:2007-12-01 00:00:00
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doi:10.1016/j.chemphyslip.2013.10.010
更新日期:2014-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2007.09.007
更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2018.03.001
更新日期:2018-07-01 00:00:00
abstract:BACKGROUND:In this study, we aimed to compare the serum lipid profile and fatty acid (FA) compositions of erythrocyte membrane (EM) and plasma in three different patient groups (group 1: type 2 diabetes mellitus (T2DM)+end-stage renal disease (ESRD), group 2: ESRD, group 4: T2DM) and healthy controls (group 3) simultan...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2013.12.011
更新日期:2014-02-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2003.10.008
更新日期:2004-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/s0009-3084(03)00092-6
更新日期:2003-11-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(84)90086-0
更新日期:1984-11-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 临床试验,杂志文章
doi:10.1016/0009-3084(94)90153-8
更新日期:1994-01-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2006.02.010
更新日期:2006-06-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
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journal_title:Chemistry and physics of lipids
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journal_title:Chemistry and physics of lipids
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更新日期:1988-03-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2010.11.005
更新日期:2011-02-01 00:00:00
abstract::The antioxidant activity during storage at 30 degrees C of quercetin, myricetin and alpha-tocopherol in small unilamellar liposomes has been investigated. Myricetin was more effective than alpha-tocopherol as an antioxidant in liposomes under all conditions studied. At pH 5.4 with a concentration of 10(-2) mol/mol pho...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/s0009-3084(98)00098-x
更新日期:1998-12-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2004.01.005
更新日期:2004-05-01 00:00:00
abstract::Cardiolipin (CL) is a phospholipid with many unique characteristics. CL is synthesized in the mitochondria and resides almost exclusively within the mitochondrial inner membrane. Unlike most phospholipids that have two fatty acyl chains, CL possesses four fatty acyl chains resulting in unique biophysical characteristi...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2015.09.005
更新日期:2015-12-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(96)02557-1
更新日期:1996-07-30 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/s0009-3084(02)00025-7
更新日期:2002-06-01 00:00:00
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journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
doi:10.1016/j.chemphyslip.2013.10.006
更新日期:2014-01-01 00:00:00
abstract::This work is focused on the study of the dynamic surface properties of spread monolayers of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), which is the main component of the pulmonary surfactant (PS), in the region of high surface pressures and at different temperatures. The increase of temperature from 25 to 35 ...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/j.chemphyslip.2019.104812
更新日期:2019-12-01 00:00:00
abstract::Fourier Transform Infra-red and Raman Spectroscopies indicate that 7 alpha-hydroxycholesterol and 7-ketocholesterol have a diminished capacity to condense (increase the packing order of) fluid-state dipalmitoylphosphatidylcholine (DPPC) acyl chains when compared with the effects of cholesterol and the other oxidized s...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章
doi:10.1016/0009-3084(86)90126-x
更新日期:1986-08-01 00:00:00
abstract::Cannabinoid research underwent a tremendous increase during the last 10 years. This progress was made possible by the discovery of cannabinoid receptors and the endogenous ligands for these receptors. Cannabinoid research is developing in two major directions: neurobehavioral properties of cannabinoids and the impact ...
journal_title:Chemistry and physics of lipids
pub_type: 杂志文章,评审
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更新日期:2000-11-01 00:00:00