Abstract:
:In this study, the role of exogenous fatty acids in the regulation of proteolipid protein (PLP) gene expression was investigated using the following model culture system: C6 glioma cells expressing the green-fluorescent protein (eGFP) driven by different segments of PLP promoter. Eicosapentanoic acid (EPA; 20:5 n-3), but not arachidonic acid (AA; 20:4 n-6), induced a significant increase in medium fluorescence intensity (MFI) determined by fluorescence-activated cell sorting (FACS). The induction of PLP promoter was time-dependent showing maximal activity between 24 and 48 h after EPA exposure. PLP promoter activation was dependent on fatty acid concentration, with maximum activation at 200 microM. Northern blot analysis confirmed the fluorescence data in C6 cells incubated with EPA. Furthermore, this treatment increased the adenylyl cyclase-cyclic AMP (cAMP) levels and the mitogen-activated protein kinase (MAPK) activation in C6 cells. PLP promoter activity was inhibited by pre-treatment with H89 (protein kinase A (PKA) inhibitor), but not with PD98059 (MAPK inhibitor), suggesting that EPA stimulates the expression of PLP via cAMP-mediated pathways.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Salvati S,Natali F,Attorri L,Raggi C,Di Biase A,Sanchez Mdoi
10.1016/s0197-0186(03)00172-4subject
Has Abstractpub_date
2004-04-01 00:00:00pages
331-8issue
5eissn
0197-0186issn
1872-9754pii
S0197018603001724journal_volume
44pub_type
杂志文章abstract::Local excitation of the hippocampus with N- methyl- d,l -aspartic acid caused a rapid release of specific proteins into the extracellular fluid. The proteins were recovered from the effluent of a small implanted tubing, permeable to macromolecules, and analyzed by sodium dodecyl sulphate electrophoresis. ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90031-8
更新日期:1986-01-01 00:00:00
abstract::Dipyridamole (DPR) and nitrobenzylthioinosine (NBI) inhibition of adenosine accumulation in synaptoneurosomes derived from rat cerebral cortex, rat cerebellum, guinea pig cerebral cortex and guinea pig cerebellum was investigated. The inhibition of adenosine accumulation by NBI was observed to be distinctly biphasic i...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(87)90055-6
更新日期:1987-01-01 00:00:00
abstract::Excitable membranes appear to be biochemically characterized by the presence of a highly specific receptor protein, which undergoes conformational changes upon stimulation and returns to its original conformation for renewed excitability. Our present knowledge of the disk membrane and its receptor protein rhodopsin is...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(80)90085-6
更新日期:1980-01-01 00:00:00
abstract::The transmitter dopamine and the protein acetylcholinesterase are released within the substantia nigra from the dendrites of nigrostriatal neurons. These phenomena do not correspond to the familiar events involved in information transfer at the classic axons synapse. It is possible that both dopamine and acetylcholine...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(85)90136-6
更新日期:1985-01-01 00:00:00
abstract::Sprague-Dawley rats exposed to uncontrollable shock can be separated by a subsequent shock escape test into two groups: a "helpless" (LH) group which demonstrates a deficit in escape behavior, and a "nonlearned helpless" (NLH) group which shows no escape deficit and acquires the escape response as readily as naive con...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(92)90065-y
更新日期:1992-07-01 00:00:00
abstract::We have used an in vitro system to monitor the effects of high levels of ammonia and ethanol on glial cells. Nuclei were isolated and the protein profiles examined by two-dimensional gel electrophoresis. Acute exposure of rat astrocyte cell cultures to ammonia or ethanol resulted in changes in cellular morphology and ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(88)90037-x
更新日期:1988-01-01 00:00:00
abstract::For elucidation of thyroid hormone-induced responsiveness of fish brain, various doses (0.012, 0.025, 0.05, 0.1, 0.25, 0.5, 1, 2 and 4 ?g/g) of triiodothyronine (T(3)) were injected in Singi fish, Heteropneustes fossilis (Bloch), for 3 consecutive days and the changes in cytosolic NADP-dependent malic enzyme (ME, EC 1...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(89)90036-3
更新日期:1989-01-01 00:00:00
abstract::On the basis of the specific [(3)H]quinuclidinyl-benzilate binding, the transport of muscarinic cholinergic receptors has been demonstrated in the ventral horn, sciatic nerve and in the 3 mm segments proximal and distal to the ligature of rat sciatic nerves ligated for 24 h (a) without electrolytic lesion, (b) six day...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(84)90036-6
更新日期:1984-01-01 00:00:00
abstract::Astrocytes, the most versatile cells of the central nervous system, play an important role in the regulation of neurotransmitter homeostasis, energy metabolism, antioxidant defenses and the anti-inflammatory response. Recently, our group characterized cortical astrocyte cultures from adult Wistar rats. In line with th...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2015.07.016
更新日期:2015-11-01 00:00:00
abstract::Striatal quinolinic acid lesions have been used as a model for the pathology seen in Huntington's disease. Seven days following a unilateral injection of 100 nmol of quinolinic acid into the rat caudate-putamen (striatum), a large increase in the binding of [3H]WIN 35,428 to the lesioned caudate-putamen was seen when ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(92)90091-5
更新日期:1992-12-01 00:00:00
abstract::Intoxication with inorganic arsenicals leads to neuropathies and impaired cognitive functions. However, little is known so far on the cellular targets that are involved in the adverse effects of arsenite to brain cells. To test whether arsenite may affect neural glucose and glutathione (GSH) metabolism, primary astroc...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2014.06.013
更新日期:2014-10-01 00:00:00
abstract::The agonist, [3H](-)[S]-1-(2-amino-2-carboxyethyl)-5-fluoro-pyrimidine-2,4-dione ([3H](S)F-Willardiine) binding to functional alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors of resealed plasma membrane vesicles and nerve endings freshly isolated from the rat cerebral cortex displayed two bind...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(03)00137-2
更新日期:2004-03-01 00:00:00
abstract::Both brain-derived neurotrophic factor (BDNF) and the serotonin receptor 2A (5-HT(2A)) have been related to depression pathology. Specific 5-HT(2A) receptor changes seen in BDNF conditional mutant mice suggest that BDNF regulates the 5-HT(2A) receptor level. Here we show a direct effect of BDNF on 5-HT(2A) receptor pr...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2009.06.013
更新日期:2009-12-01 00:00:00
abstract::The acylation of myelin proteolipid protein (PLP) and intermediate protein (IP) was investigated in an in vitro system of tissue slices prepared from actively myelinating rat brainstem. The incorporation of [(3)H]palmitate into the proteins in nine subcellular fractions including myelin and other cellular membranes wh...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(86)90148-8
更新日期:1986-01-01 00:00:00
abstract::Recent in vitro studies have revealed that benzodiazepines, opiates, barbiturates and ethanol might influence GABA binding to CNS membrane sites. Further studies of such interactions might be helpful for explaining both the mechanisms of action of these psychoactive agents and the tolerance/dependence that might resul...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(83)90062-1
更新日期:1983-01-01 00:00:00
abstract::The amyloid beta-peptide (AbetaP) is the major protein component of brain senile plaques in Alzheimer's disease. The redox state of methionine-35 residue plays a critical role in peptide neurotoxic actions. We used the fragment 31-35 of AbetaP [AbetaP(31-35)], containing a single methionine-35 residue (Met-35), to inv...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2006.03.014
更新日期:2006-10-01 00:00:00
abstract::Following intravitreal injections of (35)S-amino acids to label axonally transported proteins, calpain I and II from the superior colliculi and the lateral geniculate bodies were isolated via hydrophobic interaction chromatography, anion exchange HPLC and sodium-dodecyl sulphate electrophoresis. Among the slowly trans...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(90)90032-o
更新日期:1990-01-01 00:00:00
abstract::Aminoethylcysteine ketimine is a sulfur-containing cyclic compound produced by the enzymatic alpha-deamination of the parent aminoethylcysteine that has been detected in bovine brain and cerebellum. Aminoethylcysteine ketimine is known to dimerize spontaneously and easily lose one carboxyl group. This decarboxylated c...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(97)00094-6
更新日期:1998-04-01 00:00:00
abstract::The anticonvulsant vigabatrin (VGB; SabrilR) irreversibly inhibits GABA transaminase to increase neural GABA, yet its mechanism of retinal toxicity remains unclear. VGB is suggested to alter several amino acids, including homocarnosine, β-alanine, ornithine, glycine, taurine, and 2-aminoadipic acid (AADA), the latter ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.02.015
更新日期:2019-05-01 00:00:00
abstract::The iron siderophore binding protein lipocalin 2 (LCN2, also known as 24p3, NGAL and siderocalin) may be involved in iron homeostasis, but to date, little is known about expression of its putative receptor, brain-type organic cation transporter (BOCT, also known as BOCT1, 24p3R, NGALR and LCN2R), in the brain during n...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2015.04.009
更新日期:2015-08-01 00:00:00
abstract::The effects of ageing on the turnover of dopamine, noradrenaline and serotonin in the lateral geniculate nucleus and the visual cortex were evaluated, using high performance liquid chromatography (HPLC) with electrochemical detection. Compared to adult animals, aged rats showed more changes in the visual cortex than i...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(93)90027-3
更新日期:1993-06-01 00:00:00
abstract::Cortical cells from embryonic mice (E17) showed a rapid cell-death under the serum-free condition. The addition of nefiracetam at 0.1-10 microM increased the survival activity, while aniracetam at the same concentrations did not. The cell death was characterized to be apoptotic, since dead cells showed nuclear condens...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(01)00080-8
更新日期:2002-02-01 00:00:00
abstract::Protein kinase C (PKC) is involved in several cell events including proliferation, survival and differentiation. The aim of this work was to investigate the role of PKC activation on retinal cells proliferation. We demonstrated that PKC activation by phorbol 12-myristate 13-acetate (PMA), a tumor promoter phorbol este...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/s0197-0186(02)00059-1
更新日期:2003-01-01 00:00:00
abstract::Tyrosine hydroxylase (TH) was purified from bovine brain and enzymatically phosphorylated in vitro. Radioactively phosphorylated TH was dephosphorylated by rat tissue extracts. Of tissues examined, rat corpus striatal extracts were highest in specific activity in the TH dephosphorylating assay. Phosphorylated histone ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(83)90016-5
更新日期:1983-01-01 00:00:00
abstract::In this paper, two different aspects of growth hormone neuroregulation during aging were considered. Twenty-month-old male rats had decreased growth hormone-releasing hormone mRNA levels and a slight reduction of somatostatin mRNA levels in the hypothalamus when compared to 8-month-old counterparts. Short-term adminis...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(94)90045-0
更新日期:1994-07-01 00:00:00
abstract::The objectives of this study are to identify the mechanism of mitochondrial dysfunction during cerebral ischemic/reperfusion (I/R) injury and the therapeutic potential of tetrahydrocurcumin (THC) to mitigate mitochondrial dysfunction in experimental stroke model. In our study, 8-10 weeks old male C57BL/6 wild-type mic...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2018.11.015
更新日期:2019-01-01 00:00:00
abstract::3-Nitropropionic acid (3-NP) induces a spectrum of Huntington's disease (HD)-like neuropathologies in the rat striatum. The present study aimed to demonstrate the neuroprotective effect of lercanidipine (LER) in rats with 3-NP-induced neurotoxicity, address the possible additional protective effect of combined treatme...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.104548
更新日期:2019-12-01 00:00:00
abstract::Guanylate cyclase (GTP pyrophosphate-lyase (cyclizing); EC 4.6.1.2), in the crude synaptosomal soluble (P(2)-soluble) fraction obtained by hypo-osmotic treatment and subsequent centrifugation of crude synaptosomal (P(2)) fraction from the rat brain, showed a significantly high activity when assayed in the presence of ...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/0197-0186(81)90008-5
更新日期:1981-01-01 00:00:00
abstract::Repeated exposure to classical psychomotor stimulants, like amphetamine (AMPH), produces locomotor sensitization and accompanied structural plasticity of dendritic spines in the nucleus accumbens (NAcc). Following our previous report that repeated administration of methiopropamine (MPA), a structural analog to meth-AM...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2019.104487
更新日期:2019-10-01 00:00:00
abstract::Zinc transporter 3 (ZNT3) has been shown to transport zinc ions from the cytosol into presynaptic vesicles in the mammalian brain. Several studies have stated that the zinc ion containing synaptic vesicles of zinc-enriched neurons (ZEN) are loaded with ZNT3 proteins in their membranes. This fact makes it possible to t...
journal_title:Neurochemistry international
pub_type: 杂志文章
doi:10.1016/j.neuint.2008.02.008
更新日期:2008-06-01 00:00:00