High-yield isolation of murine microglia by mild trypsinization.

abstract：:Syndecan-1, -2, -3, and -4 are heparan sulfate proteoglycans that are differentially expressed during development and wound repair. To determine whether syndecans are also involved in brain injury, we examined the expression of syndecan core proteins genes in cryo-injured mouse brain, using in situ hybridization. All ...

journal_title：Glia

authors： Iseki K,Hagino S,Mori T,Zhang Y,Yokoya S,Takaki H,Tase C,Murakawa M,Wanaka A

更新日期：2002-07-01 00:00:00

abstract：:Cortical spreading depression (CSD) is a slowly propagating wave of depolarization of gray matter. This phenomenon is believed to underlie the migraine aura and similar waves of depolarization may exacerbate injury in a number of neurological disease states. CSD is characterized by massive ion dyshomeostasis, cell swe...

journal_title：Glia

authors： Rosic B,Dukefoss DB,Åbjørsbråten KS,Tang W,Jensen V,Ottersen OP,Enger R,Nagelhus EA

更新日期：2019-06-01 00:00:00

abstract：:Secondary progressive multiple sclerosis (SPMS) is an autoimmune disease of the central nervous system (CNS) characterized by progressive motor dysfunction, sensory deficits, and visual problems. The pathological mechanism of SPMS remains poorly understood. In this study, we investigated the role of microglia, immune ...

journal_title：Glia

authors： Tanabe S,Saitoh S,Miyajima H,Itokazu T,Yamashita T

更新日期：2019-09-01 00:00:00

abstract：:The normal adult vertebrate nervous system is a relative quiescent tissue in terms of cell proliferation. However, astrocytes in many regions of the central nervous system (CNS) retain the capacity to undergo cell division. To examine the mechanisms that regulate the proliferation of astrocytes in the CNS we have util...

journal_title：Glia

authors： Nakatsuji Y,Miller RH

更新日期：1998-04-01 00:00:00

abstract：:Zinc finger transcription factors of the Krüppel-class figure prominently in cell fate specification and differentiation in the nervous system. One of the Krüppel-type genes that was originally cloned from an oligodendrocyte library by virtue of its homology with the prototypic Krüppel motif is the rat rKr2 gene (Pott...

journal_title：Glia

authors： Lovas G,Li W,Pott U,Verga T,Hudson LD

更新日期：2001-04-15 00:00:00

abstract：:The possibility of a modular organization of non-neuronal elements was analyzed in the opercular region of the striate neocortex in adult New World monkeys. For this purpose, and in order to follow possible correlations in the general organization of neuronal and astroglial elements, immunocytochemical procedures for ...

journal_title：Glia

authors： Colombo JA,Schleicher A,Zilles K

更新日期：1999-01-01 00:00:00

abstract：:The present study describes a method to inhibit selectively the microglial cell proliferation following peripheral nerve injury. Continuous infusion of cytosine-arabinoside (ARA-C) from an osmotic minipump to the fourth ventricle or cisterna magna completely blocks the proliferation of microglial cells that normally o...

journal_title：Glia

authors： Svensson M,Aldskogius H

更新日期：1993-04-01 00:00:00

abstract：:Activation of microglia resulting in exacerbated inflammation expression plays an important role in degeneration of dopaminergic (DA) neurons in the pathogenesis of Parkinson's disease (PD). However, how this enhanced inflammation is induced in microglia remains largely unclear. Here, in the mouse PD model induced by ...

journal_title：Glia

authors： He Q,Wang Q,Yuan C,Wang Y

更新日期：2017-08-01 00:00:00

abstract：:The idea of a metabolic coupling between neurons and astrocytes in the brain has been entertained for about 100 years. The use recently of simple and well-compartmentalized nervous systems, such as the honeybee retina or purified preparations of neurons and glia, provided strong support for a nutritive function of gli...

journal_title：Glia

authors： Tsacopoulos M,Poitry-Yamate CL,Poitry S,Perrottet P,Veuthey AL

更新日期：1997-09-01 00:00:00

abstract：:The interface between the blood and the cerebrospinal fluid (CSF) is formed by the choroid plexuses (CPs), which are specialized structures located within the brain ventricles. They are composed of a vascularized stroma surrounded by a tight epithelium that controls molecular and cellular traffic between the blood and...

journal_title：Glia

authors： Nataf S,Strazielle N,Hatterer E,Mouchiroud G,Belin MF,Ghersi-Egea JF

更新日期：2006-08-15 00:00:00

abstract：:Alzheimer's disease (AD) and prion disease are characterized neuropathologically by extracellular deposits of Abeta and PrP amyloid fibrils, respectively. In both disorders, these cerebral amyloid deposits are co-localized with a broad variety of inflammation-related proteins (complement factors, acute-phase protein, ...

journal_title：Glia

authors： Eikelenboom P,Bate C,Van Gool WA,Hoozemans JJ,Rozemuller JM,Veerhuis R,Williams A

更新日期：2002-11-01 00:00:00

abstract：:Water balance between cells and extracellular compartments is essential for proper functioning of the central nervous system, as demonstrated by its perturbations in pathological conditions. Aquaporin 4 (AQP4) is the predominant water channel in brain and spinal cord, where it is present mainly on astrocytic endfeet c...

journal_title：Glia

authors： Vitellaro-Zuccarello L,Mazzetti S,Bosisio P,Monti C,De Biasi S

更新日期：2005-08-01 00:00:00

abstract：:The identification of myelin oligodendrocyte glycoprotein (MOG) as a target for autoantibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE) resulted in the re-evaluation of the role of B cell responses to myelin autoantigens in the immunopathogenesis of multiple sclerosis. MOG is a central n...

journal_title：Glia

authors： Iglesias A,Bauer J,Litzenburger T,Schubart A,Linington C

更新日期：2001-11-01 00:00:00

abstract：:Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system leading to selective destruction of myelin sheaths and/or oligodendrocytes. The immunological mechanisms responsible for myelin destruction and the primary target of the immune response have not yet been identified. Prior studies h...

journal_title：Glia

authors： Kuhlmann T,Lucchinetti C,Zettl UK,Bitsch A,Lassmann H,Brück W

更新日期：1999-10-01 00:00:00

abstract：:Oligodendrocyte precursor cells (OPCs) of the optic nerve are generated in the preoptic area, from where they migrate to colonize it entirely. Sonic hedgehog (Shh) induces the proliferation of these cells as well as influencing their migration, acting through its canonical receptor (Ptc-1). However, the multiligand re...

journal_title：Glia

authors： Ortega MC,Cases O,Merchán P,Kozyraki R,Clemente D,de Castro F

更新日期：2012-05-01 00:00:00

abstract：:Stellate ganglion neurons, important mediators of cardiopulmonary neurotransmission, are surrounded by satellite glial cells (SGCs), which are essential for the function, maintenance, and development of neurons. However, it remains unknown whether SGCs in adult sympathetic ganglia exhibit any functional diversity, and...

journal_title：Glia

authors： van Weperen VYH,Littman RJ,Arneson DV,Contreras J,Yang X,Ajijola OA

更新日期：2021-01-12 00:00:00

abstract：:Microglia contribute to pathophysiology at all stages of multiple sclerosis. Colony-stimulating factor-1 (CSF1) is crucial for microglial proliferation and activation. In this study we measured the CSF1 levels and studied its cellular expression in the mouse spinal cords with experimental autoimmune encephalomyelitis ...

journal_title：Glia

authors： Gushchina S,Pryce G,Yip PK,Wu D,Pallier P,Giovannoni G,Baker D,Bo X

更新日期：2018-10-01 00:00:00

abstract：:Vestibular schwannomas (VSs) are benign tumors that arise from the Schwann cells (SCs) lining the vestibular nerve. VS cells survive and proliferate far from neurons and axonally derived growth factors. We have previously shown that VSs produce the glial growth factor, neuregulin-1 (NRG1), and its receptors, ErbB2 and...

journal_title：Glia

authors： Hansen MR,Roehm PC,Chatterjee P,Green SH

更新日期：2006-04-15 00:00:00

abstract：:Previous studies suggest that glial cells contribute to synaptogenesis in specific neurons from the postnatal CNS. Here, we studied whether this is true for Purkinje cells (PCs), which represent a unique neuronal cell type due to their large size, massive synaptic input, and high vulnerability. Using new glia-free cul...

journal_title：Glia

authors： Buard I,Steinmetz CC,Claudepierre T,Pfrieger FW

更新日期：2010-04-01 00:00:00

abstract：:Oligodendroglia and Schwann cells synthesize myelin-specific proteins and lipids for the assembly of the highly organized myelin membrane of the motor-sensory axons in the central (CNS) and peripheral nervous system (PNS), respectively, allowing rapid saltatory conduction. The isoforms of the main myelin proteins, the...

journal_title：Glia

authors： Spörkel O,Uschkureit T,Büssow H,Stoffel W

更新日期：2002-01-01 00:00:00

abstract：:We compared the inward K+ currents of Müller glial cells from healthy and pathologically changed human retinas. To this purpose, the whole-cell voltage-clamp technique was performed on noncultured Müller cells acutely isolated from human retinas. Cells originated from retinas of four healthy organ donors and of 24 pat...

journal_title：Glia

authors： Francke M,Pannicke T,Biedermann B,Faude F,Wiedemann P,Reichenbach A,Reichelt W

更新日期：1997-07-01 00:00:00

abstract：:Injury to the adult brain results in the formation of a glial scar that involves both activated astroglia and microglia/macrophages. Although reactive gliosis has been linked to failure of axonal regeneration in the adult mammalian CNS, the spatio-temporal pattern of the postlesion responses in morphology and distribu...

journal_title：Glia

authors： Stichel CC,Müller HW

更新日期：1994-02-01 00:00:00

abstract：:Much of our present knowledge of glial cell function stems from studies of glioma cell lines, both rodent (C6, C6 polyploid, and TR33B) and human (1321N1, 138MG, D384, R-111, T67, Tp-276MG, Tp-301MG, Tp-483MG, Tp-387MG, U-118MG, U-251MG, U-373MG, U-787MG, U-1242MG, and UC-11MG). New methods such as patch clamp and Ca2...

journal_title：Glia

authors： Brismar T

更新日期：1995-11-01 00:00:00

abstract：:Inflammation has been linked to the induction of apneas and Sudden Infant Death Syndrome, whereas proinflammatory mediators inhibit breathing when applied peripherally or directly into the CNS. Considering that peripheral inflammation can activate microglia in the CNS and that this cell type can directly release all p...

journal_title：Glia

authors： Lorea-Hernández JJ,Morales T,Rivera-Angulo AJ,Alcantara-Gonzalez D,Peña-Ortega F

更新日期：2016-04-01 00:00:00

abstract：:The expression of sodium channel alpha-subunit isoforms in astrocytes cultured from P-0 rat spinal cord and P-7 rat optic nerve was examined utilizing immunocytochemical methods with antibodies generated against conserved and isoform-specific amino acid sequences of the rat brain sodium channel. In spinal cord culture...

journal_title：Glia

authors： Black JA,Westenbroek R,Minturn JE,Ransom BR,Catterall WA,Waxman SG

更新日期：1995-06-01 00:00:00

abstract：:Neurotrophin-3 (NT-3) and its receptor TrkC are known to be important for neuronal survival. More recently, NT-3 has been implicated as playing a role in oligodendrocyte (OL) proliferation and survival in vitro. Examination of NT-3 and TrkC knockout mice revealed a reduction in NT-3-dependent neurons. To date, no stud...

journal_title：Glia

authors： Kahn MA,Kumar S,Liebl D,Chang R,Parada LF,De Vellis J

更新日期：1999-04-01 00:00:00

abstract：:Microglia are sensitive to environmental changes and are immediately transformed into several phenotypes. For such dynamic "modal shifts", purinergic receptors have central roles. When microglia sense ATP/ADP leaked from injured cells by P2Y(12) receptors, they are transformed into a moving phenotype, showing process ...

journal_title：Glia

authors： Koizumi S,Ohsawa K,Inoue K,Kohsaka S

更新日期：2013-01-01 00:00:00

abstract：:Synemin, a very unique type VI intermediate filament (IF) protein, exhibits alternative splice variants termed alpha and beta. Unlike other IF proteins, synemin binds to actin-associated proteins, including alpha-actinin, vinculin, and alpha-dystrobrevin. Our previous work has demonstrated the presence of synemin in d...

journal_title：Glia

authors： Jing R,Pizzolato G,Robson RM,Gabbiani G,Skalli O

更新日期：2005-04-15 00:00:00

abstract：:Activation of adenylate cyclase in astroglial cells in culture results in a rapid change in cell shape that appears to occur by the active movement of cytoplasm from peripheral cell regions to the perinuclear space with processes being formed along regions that remain extended. Three series of experiments were designe...

journal_title：Glia

authors： Shain W,Bausback D,Fiero A,Madelian V,Turner JN

更新日期：1992-01-01 00:00:00

abstract：:Individuals with neurofibromatosis 1 (NF1) develop low-grade astrocytomas at an increased frequency. To gain insight into the function of the Nf1 gene product as a growth regulator for astrocytes, we examined mice heterozygous for a targeted Nf1 mutation. In our previous studies, we demonstrated increased numbers of p...

journal_title：Glia

authors： Bajenaru ML,Donahoe J,Corral T,Reilly KM,Brophy S,Pellicer A,Gutmann DH

更新日期：2001-03-15 00:00:00