Human metapneumovirus detection in patients with severe acute respiratory syndrome.

Abstract:

:We used a combination approach of conventional virus isolation and molecular techniques to detect human metapneumovirus (HMPV) in patients with severe acute respiratory syndrome (SARS). Of the 48 study patients, 25 (52.1%) were infected with HMPV; 6 of these 25 patients were also infected with coronavirus, and another 5 patients (10.4%) were infected with coronavirus alone. Using this combination approach, we found that human laryngeal carcinoma (HEp-2) cells were superior to rhesus monkey kidney (LLC-MK2) cells commonly used in previous studies for isolation of HMPV. These widely available HEp-2 cells should be included in conjunction with a molecular method for cell culture followup to detect HMPV, particularly in patients with SARS.

journal_name

Emerg Infect Dis

authors

Chan PK,Tam JS,Lam CW,Chan E,Wu A,Li CK,Buckley TA,Ng KC,Joynt GM,Cheng FW,To KF,Lee N,Hui DS,Cheung JL,Chu I,Liu E,Chung SS,Sung JJ

doi

10.3201/eid0909.030304

subject

Has Abstract

pub_date

2003-09-01 00:00:00

pages

1058-63

issue

9

eissn

1080-6040

issn

1080-6059

journal_volume

9

pub_type

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