Mutational analysis of a dominant oncogene (c-Ki-ras-2) and a tumor suppressor gene (p53) in hamster lung tumorigenesis.


:In human lung cancers, alterations of both a dominant oncogene (ras) and a tumor suppressor gene (p53) have been identified. Polymerase chain reaction (PCR) analysis of mRNA was used to amplify the c-Ki-ras-2 and p53 genes from Syrian golden hamsters. The PCR products were confirmed by predicted-size analysis, probing with nonradioactive (biotin-labeled) oligonucleotides, and direct sequencing. Lung tumors were produced in hamsters by repeated injections of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Of six tumors examined, three (50%) had mutations in codon 12 of Ki-ras. Examination of the conserved regions of p53 revealed no mutations. We conclude that NNK-induced carcinogenesis in the hamster results in characteristic alterations of Ki-ras but may not necessarily involve the p53 gene.


Mol Carcinog


Molecular carcinogenesis


Oreffo VI,Lin HW,Gumerlock PH,Kraegel SA,Witschi H




Has Abstract


1992-01-01 00:00:00












  • Epigenetic analysis of microRNA genes in tumors from surgically resected lung cancer patients and association with survival.

    abstract::Aberrant microRNA (miRNA) expression is involved in tumorigenesis of several cancers, including non-small cell lung cancer (NSCLC). Furthermore, expression of some miRNAs has been shown to be under epigenetic regulation. However, less is known regarding the role of miRNA methylation in NSCLC development or clinical ou...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Tan W,Gu J,Huang M,Wu X,Hildebrandt MA

    更新日期:2015-06-01 00:00:00

  • Upregulation of the long non-coding RNA HOTAIR promotes esophageal squamous cell carcinoma metastasis and poor prognosis.

    abstract::Recent studies of the individual functionalities of long non-coding RNAs (lncRNAs) in the development and progression of cancer have suggested that HOX transcript antisense RNA (HOTAIR) is capable of reprogramming chromatin organization and promoting cancer cell metastasis. In order to ascertain the expression pattern...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chen FJ,Sun M,Li SQ,Wu QQ,Ji L,Liu ZL,Zhou GZ,Cao G,Jin L,Xie HW,Wang CM,Lv J,De W,Wu M,Cao XF

    更新日期:2013-11-01 00:00:00

  • Transgenic insulin-like growth factor-1 stimulates activation of COX-2 signaling in mammary glands.

    abstract::Studies show that elevated insulin-like growth factor-1 (IGF-1) levels are associated with an increased risk of breast cancer; however, mechanisms through which IGF-1 promotes mammary tumorigenesis in vivo have not been fully elucidated. To assess the possible involvement of COX-2 signaling in the pro-tumorigenic effe...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Tian J,Lambertz I,Berton TR,Rundhaug JE,Kiguchi K,Shirley SH,Digiovanni J,Conti CJ,Fischer SM,Fuchs-Young R

    更新日期:2012-12-01 00:00:00

  • Activation and up-regulation of translation initiation factor 4B contribute to arsenic-induced transformation.

    abstract::Arsenic is a known human carcinogen. However, the mechanism of how arsenic induces cell transformation remains unclear. In this study, we demonstrated that long-term exposure to sodium arsenite at low-dose (2 µM) increases cell proliferation and neoplastic transformation in a mouse epidermal cell model, JB6 promotion-...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhang Y,Wang Q,Guo X,Miller R,Guo Y,Yang HS

    更新日期:2011-07-01 00:00:00

  • Human cDNA-expressed cytochrome P450 IA2: mutagen activation and substrate specificity.

    abstract::The vaccinia virus cDNA expression system was used to produce human cytochrome P450 IA2 in a hepatoma cell line that is devoid of significant basal levels of P450. The expressed enzyme yielded a reduced carbon monoxide-bound difference spectrum with a lambda max of 449 nm. Catalytic activities and mutagen activation a...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Aoyama T,Gonzalez FJ,Gelboin HV

    更新日期:1989-01-01 00:00:00

  • Possible involvement of c-myc but not ras genes in hepatocellular carcinomas developing after spontaneous hepatitis in LEC rats.

    abstract::LEC (Long-Evans with a cinnamon-like coat color) rats develop hepatocellular carcinomas (HCCs) spontaneously. We examined mutations of codons 12, 13, and 61 of the Ha-ras, Ki-ras, and N-ras genes in four HCCs by the polymerase chain reaction (PCR)-single-stranded DNA direct sequencing method. No ras gene mutations wer...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Fujimoto Y,Ishizaka Y,Tahira T,Sone H,Takahashi H,Enomoto K,Mori M,Sugimura T,Nagao M

    更新日期:1991-01-01 00:00:00

  • Inhibition of proliferation and expression of T1 and cyclin D1 genes by thyroid hormone in mammary epithelial cells.

    abstract::The relationship between thyroid hormone (triiodothyronine, T(3)) and breast cancer is unclear. We studied the effect of the c-erbA/TR alpha proto-oncogene encoding a functional T(3) receptor (TR alpha 1), of its ligand T(3), and of its retroviral, mutated counterpart, the v-erbA oncogene, on the proliferation capacit...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: González-Sancho JM,Figueroa A,López-Barahona M,López E,Beug H,Muñoz A

    更新日期:2002-05-01 00:00:00

  • Decreased mitogenic response to epidermal growth factor in human squamous cell carcinoma lines overexpressing epidermal growth factor receptor owing to limiting amounts of the adaptor protein Grb2: rescue by retinoic acid treatment.

    abstract::Growth factor receptors of the tyrosine kinase family regulate proliferation of a variety of cell types. In some human cancers, the epidermal growth factor receptor (EGFR) and its ligands often are overexpressed, leading to both constitutive and autocrine activation. Intracellular signaling via this receptor takes pla...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Crowe DL,Tsang KJ

    更新日期:2001-12-01 00:00:00

  • Oncogene amplification per se: an independent prognostic factor in human breast cancer.

    abstract::As the prognostic significance of the three most frequently amplified oncogenes in breast cancer (c-myc, int-2/FGF3, and c-erbB-2/neu) is still unclear, and as the amplification of these genes appears to be mutually exclusive, we investigated the prognostic significance of oncogene amplification per se by multivariate...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Champème MH,Bièche I,Hacène K,Lidereau R

    更新日期:1994-12-01 00:00:00

  • Prognostic implications of ezrin expression in human hepatocellular carcinoma.

    abstract::Ezrin is known to regulate cellular survival, adhesion, migration, and invasion and has been identified as one of the key components of tumor progression and metastasis. The authors investigated ezrin expression in human hepatocellular carcinoma (HCC) and sought to determine its relation with clinicopathologic paramet...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kang YK,Hong SW,Lee H,Kim WH

    更新日期:2010-09-01 00:00:00

  • p28(GANK) associates with p300 to attenuate the acetylation of RelA.

    abstract::Oncoprotein p28(GANK), overexpressed in hepatocellular carcinomas (HCC), binds to RelA and retains NF-κB in the cytoplasm to suppress NF-κB transactivation. However, the mechanism has not yet been elucidated. In this study, we clarified the mechanism of NF-κB regulated by p28(GANK). p28(GANK) reduced TNF-α-induced nuc...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Ren YB,Luo T,Li J,Fu J,Wang Q,Cao GW,Chen Y,Wang HY

    更新日期:2015-12-01 00:00:00

  • Molecular determinants of tumor differentiation in papillary serous ovarian carcinoma.

    abstract::In epithelial ovarian cancer, tumor grade is an independent prognosticator whose molecular determinants remain unknown. We investigated patterns of gene expression in well- and poorly differentiated serous papillary ovarian and peritoneal carcinomas with cDNA microarrays. A 6500-feature cDNA microarray was used for co...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Jazaeri AA,Lu K,Schmandt R,Harris CP,Rao PH,Sotiriou C,Chandramouli GV,Gershenson DM,Liu ET

    更新日期:2003-02-01 00:00:00

  • The nuclear receptor constitutive active/androstane receptor arrests DNA-damaged human hepatocellular carcinoma Huh7 cells at the G2/M phase.

    abstract::Here, we have demonstrated that xenobiotic activation of the nuclear receptor (CAR, NR1I3) can result in arresting DNA-damaged human hepatocellular carcinoma Huh7 cells at the G2/M phase. Huh7 cells over-expressing CAR were either treated with dimethyl sulfoxide, the CAR activator TCPOBOP (1,4-bis[2-(3,5-dichloropyrid...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kamino H,Negishi M

    更新日期:2012-02-01 00:00:00

  • Cooperative inhibitory effect of ZD1839 (Iressa) in combination with 17-AAG on glioma cell growth.

    abstract::ZD1839 ("Iressa") is an orally active, selective epidermal growth factor (EGF) receptor-tyrosine kinase inhibitor. We evaluated the antitumor activity of ZD1839 in combination with HSP90 antagonist, 17-AAG in malignant human glioma cell lines. ZD1839 independently produced a dose-dependent inhibition of cellular proli...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Premkumar DR,Arnold B,Pollack IF

    更新日期:2006-05-01 00:00:00

  • The same gamma-glutamyl transpeptidase RNA species is expressed in fetal liver, hepatic carcinomas, and rasT24-transformed rat liver epithelial cells.

    abstract::In rats, gamma-glutamyl transpeptidase (gamma GT) exists as a single-copy gene, and three distinct species of RNA (types I, II, and III) that differ in their 5' untranslated regions have been identified. To compare steady-state levels of these gamma GT RNAs in rat tissues, hepatic carcinomas, and cultured cells, we us...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Habib GM,Rajagopalan S,Godwin AK,Lebovitz RM,Lieberman MW

    更新日期:1992-01-01 00:00:00

  • Targeted disruption of Bcl-xL in mouse keratinocytes inhibits both UVB- and chemically induced skin carcinogenesis.

    abstract::Bcl-x(L) is one of several antiapoptotic proteins regulated by signal transducer and activator of transcription 3 (Stat3). We have recently shown that Stat3 is required for chemically induced and ultraviolet B (UVB)-induced skin carcinogenesis. In this study, the functional role of Bcl-x(L) in skin carcinogenesis was ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kim DJ,Kataoka K,Sano S,Connolly K,Kiguchi K,DiGiovanni J

    更新日期:2009-10-01 00:00:00

  • Changes in jun N-terminal kinase activation by stress during aging of cultured normal human fibroblasts.

    abstract::The molecular changes associated with the aging process include the reduced activity of transcription factors (such as AP-1) and an impaired response to stress, which has been well documented in the case of the heat-shock (HS) response. Using human diploid fibroblasts of early and late passages as an in vitro model fo...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Adler V,Dolan LR,Kim J,Pincus M,Barrett JC,Ronai Z

    更新日期:1996-09-01 00:00:00

  • Hyperpigmentation and melanocytic hyperplasia in transgenic mice expressing the human T24 Ha-ras gene regulated by a mouse tyrosinase promoter.

    abstract::The tyrosinase promoter has been used to target expression of the mutated human T24 Ha-ras oncogene in pigment-producing cells of transgenic mice. Two independent founder mice carrying the transgene survived and showed the same distinct phenotype of mutated coat color, deeply pigmented skin with multiple nevi, and twi...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Powell MB,Hyman P,Bell OD,Balmain A,Brown K,Alberts D,Bowden GT

    更新日期:1995-02-01 00:00:00

  • Accelerated onset of uterine tumors in transgenic mice with aberrant expression of the estrogen receptor after neonatal exposure to diethylstilbestrol.

    abstract::The role of estrogen and the estrogen receptor (ER) in the induction and promotion of tumors was investigated by using transgenic MT-mER mice, which overexpress the ER. It was hypothesized that because of this abnormal expression of the ER, the reproductive-tract tissues of the MT-mER mice may be more susceptible to t...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Couse JF,Davis VL,Hanson RB,Jefferson WN,McLachlan JA,Bullock BC,Newbold RR,Korach KS

    更新日期:1997-08-01 00:00:00

  • A Zbtb7a proto-oncogene as a novel target for miR-125a.

    abstract::In our previous study, we showed that miR-125a directly targeted a WT1 oncogene, which was overexpressed in leukemia and various kinds of solid tumors including lung, breast, gastric, and colon cancers, and brain tumors and was deeply involved in leukemogenesis and tumorigenesis and that miR-125a knockout mice overexp...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Hojo N,Tatsumi N,Moriguchi N,Matsumura A,Morimoto S,Nakata J,Fujiki F,Nishida S,Nakajima H,Tsuboi A,Oka Y,Hosen N,Hayashi S,Sugiyama H,Oji Y

    更新日期:2016-12-01 00:00:00

  • Lidamycin inhibits tumor initiating cells of hepatocellular carcinoma Huh7 through GSK3β/β-catenin pathway.

    abstract::Recently, tumor initiating cells are considered as the central role of tumorigenicity in hepatocellular carcinoma. Enediyne anticancer antibiotic lidamycin with great potential antitumor activity is currently evaluated in Phase II clinical trials. In this study, we evaluated the effect of lidamycin on tumor initiating...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Chen Y,Yu D,Zhang C,Shang B,He H,Chen J,Zhang H,Zhao W,Wang Z,Xu X,Zhen Y,Shao RG

    更新日期:2015-01-01 00:00:00

  • Susceptibility of transgenic mice carrying human prototype c-Ha-ras gene in a short-term carcinogenicity study of vinyl carbamate and ras gene analyses of the induced tumors.

    abstract::To determine if hemizygous transgenic mice carrying the human c-Ha-ras gene (CB6F1-Tg Hras2 mice (Hras2 mice)) are susceptible to the carcinogenic potential of known murine carcinogens, male and female Hras2 mice and their non-transgenic CB6F1 littermates (non-Tg mice) were each given a single intraperitoneal injectio...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Mitsumori K,Wakana S,Yamamoto S,Kodama Y,Yasuhara K,Nomura T,Hayashi Y,Maronpot RR

    更新日期:1997-11-01 00:00:00

  • Effect of energy restriction on the expression of cyclin D1 and p27 during premalignant and malignant stages of chemically induced mammary carcinogenesis.

    abstract::The restriction of energy intake has a profound inhibitory effect on carcinogenesis, yet the mechanism or mechanisms that account for this effect are unknown. In this experiment, the hypothesis tested was that energy restriction upregulates the expression of p27/kip1, a gene product associated with cell-cycle growth a...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Zhu Z,Jiang W,Thompson HJ

    更新日期:1999-04-01 00:00:00

  • circMTDH.4/miR-630/AEG-1 axis participates in the regulation of proliferation, migration, invasion, chemoresistance, and radioresistance of NSCLC.

    abstract::Astrocyte elevated gene-1 (AEG-1) plays a critical role in the development, progression, and metastasis of a variety of cancers, including non-small-cell lung cancer (NSCLC). The objective of the current study is to unravel the upstream signaling of AEG-1. A cohort of 28 NSCLC tissues and 30 normal tissues were collec...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Li YH,Xu CL,He CJ,Pu HH,Liu JL,Wang Y

    更新日期:2020-02-01 00:00:00

  • COX-2 inhibitor NS-398 suppresses doxorubicin-induced p53 accumulation through inhibition of ROS-mediated Jnk activation.

    abstract::Cyclooxygenase-2 (COX-2) is one of the isoforms of cyclooxygenase, a rate-limiting enzyme in the arachidonic acid cascade. COX-2 protein expression is highly induced by numerous factors and it has been reportedly overexpressed in various human malignancies. Although anti-tumorigenic effects of COX-2 inhibitors have be...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Kim J,Shim M

    更新日期:2016-12-01 00:00:00

  • Aberrant overexpression of FOXM1 transcription factor plays a critical role in lung carcinogenesis induced by low doses of arsenic.

    abstract::Environmental or occupational exposure to low doses of arsenic induces a series of health problems including cancer. The molecular events in arsenic-induced carcinogenicity remain to be defined. In the NuLi-1 immortalized human lung epithelial cell line with p53 and pRb deficiency, exposure to low doses of arsenic tri...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Liu Y,Hock JM,Van Beneden RJ,Li X

    更新日期:2014-05-01 00:00:00

  • Expression and localization of matrix-degrading metalloproteinases during colorectal tumorigenesis.

    abstract::The metalloproteinase matrilysin is widely expressed in the epithelial tumor cells of malignant colorectal adenocarcinomas. Approximately 50% of benign adenomas also express low levels of matrilysin that is focally localized. The expression of stromelysin-1, stromelysin-3, and gelatinase A was observed in the stromal ...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Newell KJ,Witty JP,Rodgers WH,Matrisian LM

    更新日期:1994-08-01 00:00:00

  • Magnolin targeting of ERK1/2 inhibits cell proliferation and colony growth by induction of cellular senescence in ovarian cancer cells.

    abstract::Ras/Raf/MEKs/ERKs and PI3 K/Akt/mTOR signaling pathways have key roles in cancer development and growth processes, as well as in cancer malignance and chemoresistance. In this study, we screened the therapeutic potential of magnolin using 15 human cancer cell lines and combined magnolin sensitivity with the CCLE mutao...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Song JH,Lee CJ,An HJ,Yoo SM,Kang HC,Lee JY,Kim KD,Kim DJ,Lee HS,Cho YY

    更新日期:2019-01-01 00:00:00

  • Apurinic/apyrimidinic endonuclease 1 (APE1/Ref-1) overexpression is an independent prognostic marker in prostate cancer without TMPRSS2:ERG fusion.

    abstract::Polymorphisms of the base excision repair gene APE1 may be associated with an increased risk for developing prostate cancer. In other cancer types, altered APE1 protein expression is a candidate prognostic marker. Using immunohistochemistry, we thus analyzed APE1 expression in 9763 prostate cancers in a tissue microar...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章


    authors: Juhnke M,Heumann A,Chirico V,Höflmayer D,Menz A,Hinsch A,Hube-Magg C,Kluth M,Lang DS,Möller-Koop C,Sauter G,Simon R,Beyer B,Pompe R,Thederan I,Schlomm T,Luebke AM

    更新日期:2017-09-01 00:00:00

  • Models for chromosomal replication-independent non-B DNA structure-induced genetic instability.

    abstract::Regions of genomic DNA containing repetitive nucleotide sequences can adopt a number of different structures in addition to the canonical B-DNA form: many of these non-B DNA structures are causative factors in genetic instability and human disease. Although chromosomal DNA replication through such repetitive sequences...

    journal_title:Molecular carcinogenesis

    pub_type: 杂志文章,评审


    authors: Wang G,Vasquez KM

    更新日期:2009-04-01 00:00:00