Abstract:
:In human lung cancers, alterations of both a dominant oncogene (ras) and a tumor suppressor gene (p53) have been identified. Polymerase chain reaction (PCR) analysis of mRNA was used to amplify the c-Ki-ras-2 and p53 genes from Syrian golden hamsters. The PCR products were confirmed by predicted-size analysis, probing with nonradioactive (biotin-labeled) oligonucleotides, and direct sequencing. Lung tumors were produced in hamsters by repeated injections of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK). Of six tumors examined, three (50%) had mutations in codon 12 of Ki-ras. Examination of the conserved regions of p53 revealed no mutations. We conclude that NNK-induced carcinogenesis in the hamster results in characteristic alterations of Ki-ras but may not necessarily involve the p53 gene.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Oreffo VI,Lin HW,Gumerlock PH,Kraegel SA,Witschi Hdoi
10.1002/mc.2940060305subject
Has Abstractpub_date
1992-01-01 00:00:00pages
199-202issue
3eissn
0899-1987issn
1098-2744journal_volume
6pub_type
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