Abstract:
:Both ultraviolet irradiation and oxidation of DNA produce a variety of pyrimidine base damages. A human endonuclease recognizes such altered bases on these DNA substrates. This human endonuclease incises ultraviolet-irradiated DNA exclusively at sites of photochemically modified cytosines. The precise sites of incision by the human enzyme were determined by DNA sequencing. Chemically oxidized DNA was incised exclusively at thymine loci. The degree of enzymic cleavage at cytosine photoproducts was identical at each site. However, the extent of incision at selected oxidized thymine residues varied within the DNA sequence. These results indicate that the distribution of thymine oxidative modifications is influenced by the neighboring DNA bases.
journal_name
Mol Carcinogjournal_title
Molecular carcinogenesisauthors
Gallagher PE,Weiss RB,Brent TP,Duker NJdoi
10.1002/mc.2940020404subject
Has Abstractpub_date
1989-01-01 00:00:00pages
188-91issue
4eissn
0899-1987issn
1098-2744journal_volume
2pub_type
杂志文章abstract::Formation of G4 DNA may occur in the course of replication and transcription, and contribute to genomic instability. We have quantitated abundance of G4 motifs and potential for G4 DNA formation of the nontemplate strand of 5' exons and introns of transcripts of human genes. We find that, for all human genes, G4 motif...
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