Structural requirements of C-type natriuretic peptide for elevation of cyclic GMP in cultured vascular smooth muscle cells.

abstract：:The function and intracellular localisation of the non-catalytic NH(2)-terminal region of focal adhesion kinase (FAK) are unclear. We investigated the targetting of the FAK NH(2)-terminal domain in HEK 293 and epithelial MDCK cells. Exogenous expression of a variety of GFP-fused and epitope-tagged NH(2) terminal domai...

journal_title：Biochemical and biophysical research communications

authors： Stewart A,Ham C,Zachary I

更新日期：2002-11-22 00:00:00

abstract：:P53 is renowned as a cellular tumor suppressor poised to instigate remedial responses to various stress insults that threaten DNA integrity. P53 levels and activities are kept under tight regulation involving a complex network of activators and inhibitors, which determine the type and extent of p53 growth inhibitory s...

journal_title：Biochemical and biophysical research communications

authors： Levav-Cohen Y,Goldberg Z,Zuckerman V,Grossman T,Haupt S,Haupt Y

更新日期：2005-06-10 00:00:00

abstract：:The ontogeny of the cytosolic receptor for 2,3,7,8-tetrachlorodibenzo-p-dioxin was studied in Sprague-Dawley rats by quantitation of the receptor in hepatic, lung, and thymic cytosol. Concentrations of hepatic and lung cytosolic receptors increased rapidly after birth and remained at the highest levels from days 2 to ...

journal_title：Biochemical and biophysical research communications

authors： Gasiewicz TA,Ness WC,Rucci G

更新日期：1984-01-13 00:00:00

abstract：:Mutation in DJ-1 gene is the cause of autosomal recessive Parkinson's disease, however, its physiological function remains unclear. The isoelectric point of DJ-1 shows an acidic shift after cells are treated with hydrogen peroxide. This suggests that DJ-1 is modified in response to oxidative stress. Here we report the...

journal_title：Biochemical and biophysical research communications

authors： Kinumi T,Kimata J,Taira T,Ariga H,Niki E

更新日期：2004-05-07 00:00:00

abstract：:Cultured endothelial cells release a potent vasoconstrictor peptide, endothelin. Cumulative addition of synthetic endothelin to isolated rabbit aortic rings elicited a concentration-dependent increase in contractile tension which was endothelium-independent. In cultured rabbit vascular smooth muscle cells loaded with ...

journal_title：Biochemical and biophysical research communications

authors： Marsden PA,Danthuluri NR,Brenner BM,Ballermann BJ,Brock TA

更新日期：1989-01-16 00:00:00

abstract：:Unc104/KIF1A, a kinesin family member, is reported to be monomeric in solution, though its polypeptide has regions that potentially form coiled coils. For a better understanding of the mechanism underlying Unc104/KIF1A's motility, it is important to evaluate the dimerization ability of this protein. The CD measurement...

journal_title：Biochemical and biophysical research communications

authors： Shimizu Y,Morii H,Arisaka F,Tanokura M

更新日期：2005-11-25 00:00:00

abstract：:Experimental gene transfer has permitted a wide variety of studies on gene regulation and function. However, possible effects of the introduced DNA on cellular metabolism are not well understood. Here we demonstrated that introduction of DNA into a promonocytic cell line, U937, by electroporation caused extensive cell...

journal_title：Biochemical and biophysical research communications

authors： Shimokawa T,Okumura K,Ra C

更新日期：2000-04-02 00:00:00

abstract：:Insulin inhibits hepatic very low density lipoprotein (VLDL) apo B secretion in rats. Current studies test whether the insulin effect is LDL receptor-mediated by examining the effect of insulin on VLDL apo B secretion in hepatocytes derived from Ldlr-/- and control mice. Primary hepatocytes were incubated overnight wi...

journal_title：Biochemical and biophysical research communications

authors： Chirieac DV,Cianci J,Collins HL,Sparks JD,Sparks CE

更新日期：2002-09-13 00:00:00

abstract：:Periplasmic proteins of Gram-negative bacteria like Escherichia coli are subjected to immediate affect of environmental fluctuation that may unfold proteins, due to the permeability of the outer membrane to small molecules. They are thus supposedly protected by certain molecular chaperones. Nevertheless, no homologues...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Fu X,Shen J,Zhang H,Hong W,Chang Z

更新日期：2004-04-09 00:00:00

abstract：:The presence of type I transglutaminase was determined in the neoplastic human keratinocyte line SqCC/Y1 and in normal human epidermal keratinocytes (NHEK) by an in situ radioimmunoassay which corresponded directly with the measurement of type I transglutaminase enzymatic activity. Dexamethasone induced differentiatio...

journal_title：Biochemical and biophysical research communications

authors： Moore KG,Donadio AC,Sartorelli AC

更新日期：1993-04-30 00:00:00

abstract：:Near-infrared (NIR) fluorescent dyes that are environmentally sensitive or solvatochromic are useful tools for protein labelling in in vivo biosensor applications such as glucose monitoring in diabetes since their spectral properties are mostly independent of tissue autofluorescence and light scattering, and they offe...

journal_title：Biochemical and biophysical research communications

authors： Khan F,Pickup JC

更新日期：2013-08-30 00:00:00

abstract：:Oligo/poly alpha2,8 deaminoneuraminic acid (KDN), a unique posttranslational protein modification, was found on megalin and a not yet characterized 150 kDa glycoprotein. We purified this glycoprotein from rat testis and identified it as ceruloplasmin. Furthermore, immunoprecipitated ceruloplasmin from rat thymus, ovar...

journal_title：Biochemical and biophysical research communications

authors： Ziak M,Meier M,Novak-Hofer I,Roth J

更新日期：2002-07-19 00:00:00

abstract：:It is known that NGF-responsive cells bind NGF at cell surface receptors in a specific and saturable fashion and there are two separate kinds of receptor-ligand binding interactions as judged by Rosenthal analyses. Following isolation of nerve growth factor receptors from embryonic chicken sensory ganglia, rat pheochr...

journal_title：Biochemical and biophysical research communications

authors： Lyons CR,Stach RW,Perez-Polo JR

更新日期：1983-08-30 00:00:00

abstract：:Despite their advantages in analysis, 4D NMR experiments are still infrequently used as a routine tool in protein NMR projects due to the long duration of the measurement and limited digital resolution. Recently, new acquisition techniques for speeding up multidimensional NMR experiments, such as nonlinear sampling, i...

journal_title：Biochemical and biophysical research communications

authors： Shigemitsu Y,Ikeya T,Yamamoto A,Tsuchie Y,Mishima M,Smith BO,Güntert P,Ito Y

更新日期：2015-02-06 00:00:00

abstract：:Calmodulin activation of target enzymes depends on the interaction between calmodulin hydrophobic regions and some enzyme areas. The Ca2+ induced exposure of calmodulin hydrophobic sites was studied by means of 2-p-toluidinylnaphthalene-6-sulfonate, a fluorescent probe. Scatchard and Job plots showed that the calmodul...

journal_title：Biochemical and biophysical research communications

authors： Follenius A,Gerard D

更新日期：1984-03-30 00:00:00

abstract：:Integrins alpha(M)beta(2) plays important role on leukocytes, such as adhesion, migration, phagocytosis, and apoptosis. It was hypothesized that homomeric associations of integrin subunits provide a driving force for integrins activation, and simultaneously inducing the formation of integrins clusters. However, experi...

journal_title：Biochemical and biophysical research communications

authors： Fu G,Wang C,Wang GY,Chen YZ,He C,Xu ZZ

更新日期：2006-12-29 00:00:00

abstract：:Within the first external loop of mouse and human TRESK subunits one or two N-glycosylation consensus sites were identified, respectively. Using site directed mutagenesis and Western immunoblotting a single residue of both orthologues was found to be glycosylated upon heterologous expression. Two-electrode voltage-cla...

journal_title：Biochemical and biophysical research communications

authors： Egenberger B,Polleichtner G,Wischmeyer E,Döring F

更新日期：2010-01-08 00:00:00

abstract：:Calcineurin (CN), a calcium-activated phosphatase, plays a critical role in various biological processes including T cell activation. Cabin1, a calcineurin binding protein 1, has been shown to bind directly to CN using its C-terminal region and inhibit CN activity. However, no increase in CN activity has been found in...

journal_title：Biochemical and biophysical research communications

authors： Jang H,Cho EJ,Youn HD

更新日期：2007-07-20 00:00:00

abstract：:Kinetics of permeation through connexin 43-EGFP hemichannels (Cx43-EGFP HCs) were evaluated in divalent cation-free solutions, which enhance HC open probability and thus, allow measurements during initial velocity. Three cations that become fluorescent upon binding to intracellular nucleic acids [ethidium (Etd), propi...

journal_title：Biochemical and biophysical research communications

authors： Orellana JA,Díaz E,Schalper KA,Vargas AA,Bennett MV,Sáez JC

更新日期：2011-06-17 00:00:00

abstract：:Vascular degeneration appears to play crucial roles in producing many vascular malfunctions (1-3). In order to identify specific inducers of programmed death in vascular endothelial cells (VEC), examinations were made of the effects of substances that are known to affect the vascular system by using VEC in culture (4,...

journal_title：Biochemical and biophysical research communications

authors： Araki S,Ishida T,Yamamoto T,Kaji K,Hayashi H

更新日期：1993-01-15 00:00:00

abstract：:As part of our studies on polyamine biosynthesis in yeast, the metabolism of methylthioadenosine was studied in a mutant that lacks methylthioadenosine phosphorylase (meu1delta). The nucleoside accumulates in this mutant and is mainly excreted into the culture medium. Intracellular accumulation of the nucleoside is en...

journal_title：Biochemical and biophysical research communications

authors： Chattopadhyay MK,Tabor CW,Tabor H

更新日期：2006-04-28 00:00:00

abstract：:Attachment of leukocytes to the vascular endothelium and the subsequent migration of cells into the vessel wall are early events in atherogenesis. This process requires the expression of endothelial adhesion molecules. Since tea catechins are reputed to promote antiatherogenic activities, we investigated the effects o...

journal_title：Biochemical and biophysical research communications

authors： Ludwig A,Lorenz M,Grimbo N,Steinle F,Meiners S,Bartsch C,Stangl K,Baumann G,Stangl V

更新日期：2004-04-09 00:00:00

abstract：:Adiponectin, an adipocyte-derived hormone, attenuates the production of TNFalpha by activated human macrophages. In the present study, we used porcine blood-derived macrophages to test the hypothesis that the anti-inflammatory action of adiponectin includes suppression of IL6 and an induction of IL10. Adiponectin supp...

journal_title：Biochemical and biophysical research communications

authors： Wulster-Radcliffe MC,Ajuwon KM,Wang J,Christian JA,Spurlock ME

更新日期：2004-04-09 00:00:00

abstract：:The stabilized carbonium ion salt, tropylium tetrafluoroborate, was oxidized to tropone (cycloheptatrienone) by rabbit liver aldehyde oxidase but not by the closely related molybdenum hydroxylase, xanthine oxidase. The tropylium cation is an aromatic hydrocarbon which lacks the aldehyde, imine, or iminium functional g...

journal_title：Biochemical and biophysical research communications

authors： Banks RB,Barnett SD

更新日期：1986-10-30 00:00:00

abstract：:S-Nitrosoglutathione (GSNO) is an endogenous bronchodilator levels of which are reduced in the airways of cystic fibrosis (CF) patients. GSNO has recently been shown to increase maturation of CFTR in CF cell lines at physiological concentrations. The ability of S-nitrosoglutathione to direct the DeltaF508-CFTR to the ...

journal_title：Biochemical and biophysical research communications

authors： Andersson C,Gaston B,Roomans GM

更新日期：2002-09-27 00:00:00

abstract：:The mouse NAD+-dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) gene and its 5'-flanking region was cloned from a 129 mouse ES bacteriophage P1 genomic library. The gene contains 7 exons and 6 introns and is 11.3 kb in length. The transcription initiation site was mapped at 35 bases upstream from the ATG star...

journal_title：Biochemical and biophysical research communications

authors： Matsuo M,Ensor CM,Tai HH

更新日期：1997-06-27 00:00:00

abstract：:Cell membranes are vitally important to living cells. Although the infrastructure of biological membrane is provided by the lipid bilayer, membrane proteins perform most of the specific functions. Knowledge of membrane protein types often provides crucial hints toward determining the function of an uncharacterized mem...

journal_title：Biochemical and biophysical research communications

authors： Liu H,Wang M,Chou KC

更新日期：2005-10-28 00:00:00

abstract：:beta-Amyloid (Abeta) is the primary protein component of senile plaques in Alzheimer's disease (AD) and has been implicated in neurotoxicity associated with the disease. Abeta aggregates readily in vitro and in vivo, and its toxicity has been linked to its aggregation state. Prevention of Abeta aggregation has been in...

journal_title：Biochemical and biophysical research communications

authors： Lee S,Carson K,Rice-Ficht A,Good T

更新日期：2006-08-25 00:00:00

abstract：:2-Hydroxyheptanal (2-HH) is one of the reactive aldehyde species generated during the peroxidation of n-6 polyunsaturated fatty acids, such as linoleic and arachidonic acids. Analogous to the Maillard reaction of reducing sugars, 2-HH readily reacts with lysine epsilon-amino groups. In the present study, to define the...

journal_title：Biochemical and biophysical research communications

authors： Itakura K,Furuhata A,Shibata N,Kobayashi M,Uchida K

更新日期：2003-08-29 00:00:00

abstract：:Calcineurin Inhibitors (CNIs) are routinely used for immunosuppression following solid organ transplantation. However, the prolonged use of these agents lead to organ fibrosis which limits their efficacy. CNIs induce TGFβ expression, which is reported to augment endothelial-to-mesenchymal transition (EndMT), but their...

journal_title：Biochemical and biophysical research communications

authors： Woda CB,Bruneau S,Mak AL,Haskova Z,Liu K,Ghosh CC,Briscoe DM

更新日期：2019-11-19 00:00:00