Abstract:
BACKGROUND:The balance between apoptosis and proliferation of vascular smooth muscle cells (VSMCs) is believed to contribute to the vascular remodeling process. Cyclic AMP response element-binding protein (CREB) is a critical transcription factor for the survival of neuronal cells and T lymphocytes. However, the role of CREB in blood vessels is incompletely characterized. METHODS AND RESULTS:Nuclear staining with Hoechst 33258 or propidium iodine showed an increase in apoptotic cells with activation of caspase-3 in VSMCs infected with adenovirus expressing the dominant-negative form of CREB (AdCREBM1). Basal expression of Bcl-2 and Bcl-2 promoter activity were decreased by infection with AdCREBM1. Immunohistochemistry revealed that CREB was mainly induced and activated in the neointimal alpha-smooth muscle actin-positive cells of rat carotid artery after balloon injury. Infection with AdCREBM1 suppressed neointimal formation (intima-media ratio) by 33.8% after 14 days of injury, which was accompanied by an increase in apoptosis as indicated by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling-positive cells and a decrease in bromodeoxyuridine incorporation. CONCLUSIONS:These results suggest that CRE-dependent gene transcription might play an important role in the survival and proliferation of VSMCs. CREB might be a novel transcription factor mediating the vascular remodeling process and a potential therapeutic target for atherosclerotic disease.
journal_name
Circulationjournal_title
Circulationauthors
Tokunou T,Shibata R,Kai H,Ichiki T,Morisaki T,Fukuyama K,Ono H,Iino N,Masuda S,Shimokawa H,Egashira K,Imaizumi T,Takeshita Adoi
10.1161/01.CIR.0000085164.13439.89subject
Has Abstractpub_date
2003-09-09 00:00:00pages
1246-52issue
10eissn
0009-7322issn
1524-4539pii
01.CIR.0000085164.13439.89journal_volume
108pub_type
杂志文章相关文献
CIRCULATION文献大全abstract::Direct examination of the aortic orifice at the level of the aortic valves (aortic valvular orifice area, AVOA) in the short-axis plane was performed with a 3 MHz two-dimensional pulsed Doppler echocardiographic apparatus. The AVOA was mapped with the Doppler gate to detect or rule out the presence of a regurgitant ao...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.68.5.998
更新日期:1983-11-01 00:00:00
abstract::We examined the influence of left ventricular end-diastolic pressure (LVEDP) on the mechanical interaction between ischemic and nonischemic areas during acute myocardial ischemia. Circumferentially oriented ultrasonic segment gauges were implanted in the midwall of the anterior apex and posterior apex of the left vent...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.72.5.1125
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND:DC shock catheter ablation for the treatment of ventricular tachycardia (VT) may induce VT episodes that disappear within days. METHODS AND RESULTS:A 30-J cathodal shock was delivered to the endocardial left ventricular wall in 15 closed-chest dogs. All dogs had VT during the first day after ablation. Eleve...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.84.1.267
更新日期:1991-07-01 00:00:00
abstract:BACKGROUND:C-reactive protein (CRP), a well-recognized marker of atherosclerosis, has recently been suggested to have a direct proinflammatory effect. The constitutive expression of low levels of CRP in normal plasma suggests the likelihood that a natural factor exists to neutralize the effect of CRP. This factor(s) ha...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000127419.45975.26
更新日期:2004-05-04 00:00:00
abstract::After addition of a low concentration of thrombin to normal plasma, a progressive and significant increase in crosslinked fibrin polymers was found by sodium dodecyl sulfate agarose gel electrophoresis, reaching 27% of total fibrinogen and fibrin before gel formation. As measured by enzyme-linked immunosorbent assay w...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.79.3.666
更新日期:1989-03-01 00:00:00
abstract::Normal right ventricular muscles and similar preparations obtained from cats with signs of subacute right ventricular failure (RVF) (3 to 14 days after pressure overload induced by pulmonary artery coarctation) were studied during stimulation (at 0.5 Hz) and exposure to high-K+ Tyrode's solution. Twitch force, rate of...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1986-03-01 00:00:00
abstract:BACKGROUND:High blood pressure (BP) is a major risk factor for cardiovascular diseases (CVDs), the leading cause of mortality worldwide. Both heritable and lifestyle risk factors contribute to elevated BP levels. We aimed to investigate the extent to which lifestyle factors could offset the effect of an adverse BP gene...
journal_title:Circulation
pub_type: 临床试验,杂志文章
doi:10.1161/CIRCULATIONAHA.117.030898
更新日期:2018-02-13 00:00:00
abstract:BACKGROUND:Oxidative stress has been implicated in vascular injury and atherogenesis, and antioxidant treatment has shown favorable results in preclinical studies. Despite this, antioxidant therapy has failed to show benefit in clinical trials. Failure of antioxidants in clinical trials may be partly because such thera...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.CIR.0000128034.70732.C2
更新日期:2004-05-25 00:00:00
abstract:BACKGROUND:NAD(P)H oxidases are important sources of superoxide in the vasculature, the activity of which is associated with risk factors for human atherosclerosis. This study was designed to investigate the localization of superoxide production and the expression of the Nox family of NAD(P)H oxidase proteins (gp91phox...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.0000012917.74432.66
更新日期:2002-03-26 00:00:00
abstract::Diffuse coronary artery disease is frequently untreatable by coronary artery bypass or angioplasty. Many such "no-option" patients have been subjects for trials of angiogenesis using growth factor manipulation or laser injury. We think these novel revascularization strategies are limited by insufficient inflow to puta...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.103.21.2539
更新日期:2001-05-29 00:00:00
abstract::To determine if acute lung rejection after heart-lung transplantation can be detected noninvasively with indium-111-labeled (111In) lymphocytes, we studied 33 allogeneic and 18 isogeneic heterotopic heart-lung transplants in rats. Twenty-four hours after injection of isogeneic splenic 111In-lymphocytes (75 +/- 2 micro...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::The continuity equation suggests that a ratio of velocities at two different cardiac valves is inversely proportional to the ratio of cross-sectional areas of the valves. To determine whether a ratio of mitral/aortic valve orifice velocities is useful in determining aortic valve area in patients with aortic stenosis, ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.73.5.964
更新日期:1986-05-01 00:00:00
abstract::Blood pressure and heart rate of 15 male shift workers were measured every 15 minutes for 24 hours during three work shifts: morning, 4:00 AM to noon; afternoon, noon to 8:00 PM; and night, 8:00 PM to 4:00 AM. For each shift, 24-hour systolic and diastolic blood pressure showed a large "trough" (the low pressure span)...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.80.2.341
更新日期:1989-08-01 00:00:00
abstract:BACKGROUND:Approximately half of all patients with chronic heart failure (HF) have a decreased ejection fraction (EF) (systolic HF [SHF]); the other half have HF with a normal EF (diastolic HF [DHF]). However, the underlying pathophysiological differences between DHF and SHF patients are incompletely defined. The purpo...
journal_title:Circulation
pub_type: 杂志文章,随机对照试验
doi:10.1161/CIRCULATIONAHA.108.783910
更新日期:2008-09-30 00:00:00
abstract:BACKGROUND:Several of the theories on the pathogenesis of atherosclerosis may be integrated into a single multifactorial one. According to this theory, the most likely sequence of events involved in early atherosclerosis is vascular dysfunction and/or injury, monocyte recruitment and macrophage formation, lipid deposit...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:
更新日期:1993-03-01 00:00:00
abstract:BACKGROUND:Angiotensin II (Ang II) plays a critical role in the development of vascular lesions in hypertension, atherosclerosis, and several renal diseases. Because Ang II may contribute to the leukocyte recruitment associated with these pathological states, the aim of the present study was to assess the role of Ang I...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.102.17.2118
更新日期:2000-10-24 00:00:00
abstract::Heart failure with preserved ejection fraction (HFpEF) is a complex syndrome with an increasingly recognized heterogeneity in pathophysiology. Exercise intolerance is the hallmark of HFpEF and appears to be caused by both cardiac and peripheral abnormalities in the arterial tree and skeletal muscle. Mitochondrial abno...
journal_title:Circulation
pub_type: 杂志文章,评审
doi:10.1161/CIRCULATIONAHA.118.036259
更新日期:2019-03-12 00:00:00
abstract::We report noninvasive and invasive studies designed to clarify the mechanism of the third heart sound (S3) in humans. The noninvasive observations were made using a miniature accelerometer attached to the skin surface at the cardiac apex. In subjects with no S3, the tracings were either flat or showed very low undulat...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.67.2.399
更新日期:1983-02-01 00:00:00
abstract:BACKGROUND:Tumor necrosis factor-alpha (TNF-alpha) and angiotensin II (Ang II) modulate heart failure in part by provoking the hypertrophic response. Signal transduction pathways of those factors are implicated in reactive oxygen intermediates (ROIs). Therefore, we hypothesized that TNF-alpha and Ang II might cause myo...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.98.8.794
更新日期:1998-08-25 00:00:00
abstract:BACKGROUND:Rel/NF-kappaB, an oxidative stress-responsive transcription factor, participates transiently in the control of gene expression. The cellular mechanisms that mediate NF-kappaB activation during ischemia (and during reperfusion in the course of treating ischemia) are not known. METHODS AND RESULTS:To investig...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.100.suppl_2.ii-361
更新日期:1999-11-09 00:00:00
abstract:BACKGROUND:A mortality follow-up of 12,866 men was conducted 16 years after randomization to special intervention (SI) or usual care (UC) groups of the Multiple Risk Factor Intervention Trial to assess the long-term effect of cardiovascular risk factor intervention on coronary heart disease (CHD), cardiovascular death ...
journal_title:Circulation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1161/01.cir.94.5.946
更新日期:1996-09-01 00:00:00
abstract:BACKGROUND:The relation between left ventricular (LV) oxygen consumption (MVO2) and pressure-volume area (PVA) developed in isolated hearts provides a powerful method to understand cardiac energetics. We investigated application of this relation to the intact circulation, determining its response to steady-state and tr...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.89.2.810
更新日期:1994-02-01 00:00:00
abstract::Experience with percutaneous transluminal coronary angioplasty (PTCA) of multiple vessels was reviewed to assess short-term outcome and long-term results. PTCA of multiple vessels was performed in 100 of the initial 500 patients (20%) who underwent PTCA at the Medical College of Virginia between July 1979 and August 1...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.72.6.1314
更新日期:1985-12-01 00:00:00
abstract:BACKGROUND:Higher carotid intimal-medial thickness (IMT) is associated with cardiovascular risk factors and is predictive of coronary artery disease and stroke in older adults. Carotid IMT was measured in young and middle-aged adults to determine its relationship with risk factors measured (1) in childhood, (2) current...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/hc4601.099486
更新日期:2001-12-04 00:00:00
abstract:BACKGROUND:Continuous-flow rotary pumps with axial design are increasingly used for left ventricular assist support. The efficacy of this design compared with pulsatile, volume displacement pumps, with respect to characteristics of left ventricular unloading, and exercise performance remains largely unstudied. METHODS...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.106.677898
更新日期:2007-09-11 00:00:00
abstract:BACKGROUND:Imaging of the flow convergence region (FCR) proximal to a regurgitant orifice has been shown to provide a method for quantifying the regurgitant flow rate. According to the continuity principle, the FCR is constituted by concentric hemispheric isovelocity surfaces centered at the orifice. The flow rate is c...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.84.4.1481
更新日期:1991-10-01 00:00:00
abstract::One hundred eighteen consecutive patients undergoing valve replacement for aortic stenosis were analyzed to determine the incidence of and predisposing factors to postoperative atrial tachyarrhythmias. Univariate and multivariate analyses were performed on 70 clinical, hemodynamic, radiographic, electrocardiographic, ...
journal_title:Circulation
pub_type: 杂志文章
doi:
更新日期:1985-09-01 00:00:00
abstract::Real-time cross-sectional echocardiographic studies of the main coronary arteries were performed in 20 normal subjects, 12 patients with patent ductus arteriosus and 14 patients with coronary artery fistula in whom the diagnosis was established by angiography. In 12 patients, the coronary artery that formed the fistul...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.65.3.600
更新日期:1982-03-01 00:00:00
abstract:BACKGROUND:Reperfusion of acutely infarcted myocardium may be beneficial in limiting infarct expansion and ventricular remodeling even if established after the time that salvage of ischemic myocardium is possible. METHODS AND RESULTS:To examine the permanency, time course, and mechanism of this effect of late reperfus...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/01.cir.88.6.2872
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:Although acute rheumatic fever (ARF) and its sequel, rheumatic heart disease (RHD), continue to cause a large burden of morbidity and mortality in disadvantaged populations, most studies investigating the effectiveness of control programs date from the 1950s. A control program, including a disease register, ...
journal_title:Circulation
pub_type: 杂志文章
doi:10.1161/CIRCULATIONAHA.113.001477
更新日期:2013-07-30 00:00:00