Structural and functional studies on phi 29 DNA polymerase.

abstract：:In Drosophila as in many organisms beta tubulins are encoded by a gene family. We have determined the complete nucleotide sequences coding for the beta 1 and beta 2 tubulins of Drosophila melanogaster and the beta 2 tubulin of D. hydei, and found these insect beta tubulins to be highly conserved and like beta tubulins...

journal_title：Chromosoma

authors： Michiels F,Falkenburg D,Müller AM,Hinz U,Otto U,Bellmann R,Glätzer KH,Brand R,Bialojan S,Renkawitz-Pohl R

更新日期：1987-01-01 00:00:00

abstract：:Macronuclear DNA was isolated from Paramecium primaurelia, stock 168. Although the macronucleus is polyploid to the extent of 840C, in other respect the DNA appears to be simply organized, having neither satellite sequences nor substantial amounts of intermediately repetitive sequence. The sequence complexity of macro...

journal_title：Chromosoma

authors： McTavish C,Sommerville J

更新日期：1980-01-01 00:00:00

abstract：:Nuclear and cytoplasmic RNA of Stylonychia mytilus were analyzed on denaturing polyacrylamide gels. The molecular weight of rRNA precursor molecules is within a range of 2.1 x 10(6) daltons. A comparison between the electrophoretic pattern of nuclear non-ribosomal RNA and cytoplasmic mRNA indicates that a considerable...

journal_title：Chromosoma

authors： Nock A

更新日期：1981-01-01 00:00:00

abstract：:We have characterized two post-translational histone modifications in Caenorhabditis elegans on a genomic scale. Micrococcal nuclease digestion and immunoprecipitation were used to obtain distinct populations of single nucleosome cores, which were analyzed using massively parallel DNA sequencing to obtain positional a...

journal_title：Chromosoma

authors： Gu SG,Fire A

更新日期：2010-02-01 00:00:00

abstract：:Histone modifications regulate key processes of eukaryotic genomes. Misregulation of the enzymes that place these modifications can lead to disease. An example of this is DOT1L, the enzyme that can mono-, di-, and trimethylate the nucleosome core on lysine 79 of histone H3 (H3K79). DOT1L plays a role in development an...

journal_title：Chromosoma

authors： Vlaming H,van Leeuwen F

更新日期：2016-09-01 00:00:00

abstract：:The molecular structure of chromatin during dogfish spermiogenesis was examined by electron microscopy after the dispersion of nuclei at low ionic strength. In early and late stages of differentiation (round and elongating spermatids), chromatin is globular, although basic nuclear proteins are different from those pre...

journal_title：Chromosoma

authors： Gusse M,Chevaillier P

更新日期：1980-01-01 00:00:00

abstract：:Using fluorescence in situ hybridization, conventional epifluorescence microscopy, and laser scanning confocal microscopy followed by three-dimensional reconstruction we describe a well-defined higher order packaging of the human genome in the sperm cell nucleus. This was determined by the spatial localization of cent...

journal_title：Chromosoma

authors： Zalensky AO,Allen MJ,Kobayashi A,Zalenskaya IA,Balhórn R,Bradbury EM

更新日期：1995-05-01 00:00:00

abstract：:The members of the serine-arginine (SR) family of proteins play multiple roles in posttranscriptional gene expression. Initially considered as essential splicing factors confined to the nucleus and regulating constitutive and alternative splicing, SR proteins are now known to shuttle between the nucleus and the cytopl...

journal_title：Chromosoma

authors： Singh OP,Visa N,Wieslander L,Daneholt B

更新日期：2006-12-01 00:00:00

abstract：:The large-scale primary structure of the centromeric region of rice chromosome 5 was analyzed, the first example in a cereal species. The yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) contigs aligned on the centromere of rice chromosome 5 (CEN5) covered a distance of more than 670 kb. Str...

journal_title：Chromosoma

authors： Nonomura K,Kurata N

更新日期：2001-08-01 00:00:00

abstract：:Exposure of Nyctotherus ovalis to low temperatures or vinblastine caused similar reactions of "classes" of microtubules (mt) present in the mitotic micronucleus of this ciliate towards both treatments. However, differences of sensitivity between certain "classes" of mt at individual mitotic stages exist. Unlike the ki...

journal_title：Chromosoma

authors： Eichenlaub-Ritter U,Ruthmann A

更新日期：1982-01-01 00:00:00

abstract：:A plant carrying a small extra chromosome was found in Landsberg erecta ecotype of Arabidopsis thaliana. Fluorescence in situ hybridization revealed that this minichromosome was derived from the short arm of chromosome 4. The size of this "mini4S" chromosome was estimated to be approximately 7.5 Mb on the basis of pre...

journal_title：Chromosoma

authors： Murata M,Shibata F,Yokota E

更新日期：2006-08-01 00:00:00

abstract：:Studies performed on human trisomic 21 oocytes have revealed that during meiosis, the three homologues 21 synapse and, in some cases, achieve what looks like a trivalent. This implies that meiotic recombination takes place among the three homologous chromosomes 21, and to some extent, crossovers form between them. To ...

journal_title：Chromosoma

authors： Robles P,Roig I,Garcia R,Brieño-Enríquez M,Martin M,Cabero L,Toran N,Garcia Caldés M

更新日期：2013-03-01 00:00:00

abstract：:The irradiation of metaphase spreads of human cells with ultraviolet (UV) light blocked the chromosome banding induced by Alu I, Mbo I, Dde I, Hinf I, Hae III, and Rsa I restriction endonucleases. At 13 J/m2 there was moderate inhibition of the nuclease action, which was detected as an increase in the stain intensity ...

journal_title：Chromosoma

authors： Bianchi NO,Bianchi MS,Cleaver JE

更新日期：1984-01-01 00:00:00

abstract：:Recent progress in elucidating the function of synaptonemal complex (SC) proteins and of cohesins in meiocytes made possible, in particular, through the analysis of mice deficient in SC or cohesin proteins has significantly enriched our understanding of how meiotic chromosome architecture is determined. Cohesins and t...

journal_title：Chromosoma

authors： Revenkova E,Jessberger R

更新日期：2006-06-01 00:00:00

abstract：:Among his many contributions to the field of chromosome structure and dynamics, J. Herbert Taylor showed that eukaryotic cells have many more potential replication origins than they use, which they can recruit when replication forks are slowed to complete S-phase in a timely fashion. Thirty years later, his findings r...

journal_title：Chromosoma

authors： Gilbert DM

更新日期：2007-08-01 00:00:00

abstract：:In order to induce chromosomal rearrangements, males were exposed to x-rays and then mated to non-irradiated females. The number of each type of structural alteration was determined by examination of the polytene chromosomes of the F1 progeny. -- A comparison of the results with similar studies made on Drosophila reve...

journal_title：Chromosoma

authors： Israelewski N

更新日期：1975-12-10 00:00:00

abstract：:A complete human metaphase chromosome has been reconstructed from a series of electron microscopical projections obtained by tilting the specimen stage at 3 degree intervals from -60 to +60 degrees. The reconstructed structure is about 3.0 microns long, 1.6 micron wide, and 0.8 micron thick. The mass distribution was ...

journal_title：Chromosoma

authors： Harauz G,Borland L,Bahr GF,Zeitler E,van Heel M

更新日期：1987-01-01 00:00:00

abstract：:The distribution of quinacrine and protein sulphur has been compared with that of DNA in euchromatic and heterochromatic regions of mouse chromosomes stained with the fluorescent dye quinacrine, using X-ray microanalysis. Heterochromatin tends to bind relatively more quinacrine than euchromatin, and contains a greater...

journal_title：Chromosoma

authors： Sumner AT

更新日期：1985-01-01 00:00:00

abstract：:Meiosis is a conserved cell division process that is used by sexually reproducing organisms to generate haploid gametes. Males and females produce different end products of meiosis: eggs (females) and sperm (males). In addition, these unique end products demonstrate sex-specific differences that occur throughout meios...

journal_title：Chromosoma

authors： Cahoon CK,Libuda DE

更新日期：2019-09-01 00:00:00

abstract：:The antigens in HeLa and Trichosia pubescens cells, recognized by sera from patients with rheumatic diseases containing anti-Ku antibodies, were compared by means of immunoprecipitation of labeled cell extracts. The autoantibodies present in the tested sera precipitate at least two polypeptides of approximately Mr = 7...

journal_title：Chromosoma

authors： Amabis JM,Amabis DC,Kaburaki J,Stollar BD

更新日期：1990-04-01 00:00:00

abstract：:The ribosome is responsible for the final step of decoding genetic information into proteins. Therefore, correct assembly of ribosomes is a fundamental task for all living cells. In eukaryotes, the construction of the ribosome which begins in the nucleolus requires coordinated efforts of >350 specialized factors that ...

journal_title：Chromosoma

authors： Gerhardy S,Menet AM,Peña C,Petkowski JJ,Panse VG

更新日期：2014-08-01 00:00:00

abstract：:We describe a novel rearranged human Y chromosome consisting of an inverted duplication of the long arm heterochromatin and a small amount of euchromatin: rea(Y)(qter-q11.2::q11.2-qter). The normal centromere has been deleted and a neocentromere containing CENP-A, -C, -E and Mad2 but not CENP-B has formed close to the...

journal_title：Chromosoma

authors： Floridia G,Gimelli G,Zuffardi O,Earnshaw WC,Warburton PE,Tyler-Smith C

更新日期：2000-01-01 00:00:00

abstract：:Homologous chromosomes exchange genetic information through recombination during meiosis, a process that increases genetic diversity, and is fundamental to sexual reproduction. In an attempt to shed light on the dynamics of mammalian recombination and its implications for genome organization, we have studied the recom...

journal_title：Chromosoma

authors： Ruiz-Herrera A,Vozdova M,Fernández J,Sebestova H,Capilla L,Frohlich J,Vara C,Hernández-Marsal A,Sipek J,Robinson TJ,Rubes J

更新日期：2017-10-01 00:00:00

abstract：:Isolated nuclei of the unicellular alga Olisthodiscus luteus, the uninucleate dinoflagellate Crypthecodinium cohnii and the binucleate dinoflagellate Peridinium balticum were lysed and deposited on grids by the microcentrifugation technique. The ultrastructure of the released chromatin fibers was compared to that of m...

journal_title：Chromosoma

authors： Rizzo PJ,Burghardt RC

更新日期：1980-01-01 00:00:00

abstract：:Almost half of the human genome consists of repetitive DNA. Understanding what role these elements have in setting up chromatin states that underlie gene and chromosome function in complex genomes is paramount. The function of some types of repetitive DNA is obvious by virtue of their location, such as the alphoid arr...

journal_title：Chromosoma

authors： Chadwick BP

更新日期：2009-12-01 00:00:00

abstract：:Dioecious species accounted for 6% of all plant species, including a number of crops and economically important species, such as poplar. However, sex determination and sex chromosome evolution have been studied only in few dioecious species. In poplar, the sex-determining locus was mapped to chromosome 19. Interesting...

journal_title：Chromosoma

authors： Xin H,Zhang T,Han Y,Wu Y,Shi J,Xi M,Jiang J

更新日期：2018-09-01 00:00:00

abstract：:Feulgen cytophotometric measurements of neuroblasts in the first and third instar larvae of Drosophila melanogaster reveal the same DNA content for metaphases with chromosomes of different size. The total absorbance of all measured metaphases gives the four-fold value of that of the spermatids. Accordingly there seem ...

journal_title：Chromosoma

authors： van de Flierdt K

更新日期：1975-01-01 00:00:00

abstract：:Fluorescent in situ hybridization with chromosome specific probes was used in conjunction with laser scanning confocal microscopy to assess the three-dimensional distribution of chromosomes in human T-lymphocyte nuclei. Cells in the G1-phase of the cell cycle exhibit a distinctly non-random chromosome organization:cen...

journal_title：Chromosoma

authors： Ferguson M,Ward DC

更新日期：1992-08-01 00:00:00

abstract：:The mean DNA content of G2 macronuclei varies during the life cycle of the ciliate Tetrahymena thermophila. Early in the life cycle the mean is about 130 C; later it is about 94 C. In hybrids between strains A and B the decrease from 130 C to 94 C usually began after 60 fissions after conjugation. In B X B clones the ...

journal_title：Chromosoma

authors： Doerder FP,DeBault LE

更新日期：1978-10-20 00:00:00

abstract：:Using electron spectroscopic imaging, a new type of small granular structural constituent has been observed in the extrachromosomal zone of the polytene nucleus of the salivary gland cells of Chironomus thummi and Chironomus tentans. These granules appear isolated or in small clumps and are often seen to be connected ...

journal_title：Chromosoma

authors： Vázquez-Nin GH,Echeverría OM,Rouelle-Rossier VB,Fakan S

更新日期：1993-12-01 00:00:00