Abstract:
:The transcription factor LMO2 is believed to exert its effect through the formation of protein-protein interactions with other DNA-binding factors such as GATA-1 and TAL1. Although LMO2 has been shown to be critical for the formation of the erythroid cell lineage, the gene is also expressed in a number of nonerythroid tissues. In this report, we demonstrate that the more distal of the 2 promoters for the LMO2 gene is highly restricted in its pattern of expression, directing the hematopoietic-specific expression of this gene. Deletion and mutation analyses have identified a critical cis element in the first untranslated exon of the gene. This element is a consensus-binding site for a small family of basic leucine zipper proteins containing a proline and acidic amino acid-rich (PAR) domain. Although all 3 members of this family are produced in erythroid cells, only 2 of these proteins, thyrotroph embryonic factor and hepatic leukemia factor, can activate transcription from this LMO2 promoter element. These findings represent a novel mechanism in erythroid gene regulation because PAR proteins have not previously been implicated in this process.
journal_name
Bloodjournal_title
Bloodauthors
Crable SC,Anderson KPdoi
10.1182/blood-2002-09-2702subject
Has Abstractpub_date
2003-06-15 00:00:00pages
4757-64issue
12eissn
0006-4971issn
1528-0020pii
2002-09-2702journal_volume
101pub_type
杂志文章相关文献
BLOOD文献大全abstract::Using age-fractionated erythrocytes, warm autoantibodies can be classified into two distinct categories, depending on their reactivity with reticulocyte-enriched (younger) or reticulocyte-poor (older) red cell fractions. The strength of the direct antiglobulin test (DAT) on the age-fractionated red cells of 24 patient...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-01-01 00:00:00
abstract::Peripheral blood leukemia cells from patients with acute monoblastic leukemia (AMoL) were tested for killer cell activity against target cells that detected natural killer cell-mediated or monocyte-mediated spontaneous cytotoxicity. The fibrosarcoma cell line Wehi 164, pretreated with actinomycin D to induce susceptib...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Light-mediated release of signaling ligands, such as chemoattractants, growth factors, and cytokines is an attractive strategy for investigation and therapeutic targeting of leukocyte communication and immune responses. We introduce a versatile optogenetic method to control ligand secretion, combining UV-conditioned e...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-11-684852
更新日期:2016-06-09 00:00:00
abstract::Mammalian erythrocytes undergo a unique maturation process in which they discard their nuclei and organelles and assume a flexible biconcave shape. We found that altered plasma lipoprotein metabolism can profoundly influence these events. Abnormal erythrocyte morphology was observed in hypercholesterolemic mice lackin...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.5.1817
更新日期:2002-03-01 00:00:00
abstract::Chronic myeloid leukemia (CML) patients with the BCR-ABL T315I mutation do not benefit from therapy with currently approved tyrosine kinase inhibitors. Omacetaxine mepesuccinate is a protein synthesis inhibitor that has demonstrated activity in cells harboring the T315I mutation. This phase 2 trial assessed the effica...
journal_title:Blood
pub_type: 杂志文章,多中心研究
doi:10.1182/blood-2012-03-415307
更新日期:2012-09-27 00:00:00
abstract::RANTES (CCL5) is a chemokine implicated in many human diseases. We previously showed that the transcription factor Kruppel-like factor 13 (KLF13) controls the late (3-5 days after activation) expression of RANTES in T lymphocytes and that KLF13 itself is translationally regulated through the 5'-untranslated region of ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-03-415968
更新日期:2012-08-23 00:00:00
abstract::We developed 2bF9 transgenic mice in a hemophilia B mouse model with the expression of human factor IX (FIX) under control of the platelet-specific integrin alphaIIb promoter, to determine whether ectopically expressing FIX in megakaryocytes can enable the storage of FIX in platelet alpha-granules and corrects the mur...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-11-255612
更新日期:2010-08-26 00:00:00
abstract::Purified human high-mol-wt kininogen (HMWK), the cofactor of the contact phase of blood coagulation, migrated as a single band (approximately 110,000 mol wt) in a continuous buffer sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), but appeared as two separated bands (approximately 120,000 and 105,0...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Many surface receptors and signaling molecules are thought to associate with unique membrane microdomains termed lipid rafts. We examined the involvement of lipid rafts in the activation of leukocyte function-associated antigen-1 (LFA-1). Depletion or sequestration of cholesterol with methyl-beta-cyclodextrin (MCD) or...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3195
更新日期:2003-07-01 00:00:00
abstract::We have determined the 2905 nucleotide sequence of the rhesus macaque factor IX complementary DNA (cDNA) and found it to be greater than 95% identical to that of the human factor IX cDNA. The cDNA has a large 3' untranslated region like the human cDNA, but unlike the human cDNA has two polyadenylation sites 224 nucleo...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-03-15 00:00:00
abstract::Activating mutations of the Fms-like tyrosine kinase 3 (FLT3) receptor are the most common genetic alteration in acute myeloid leukemia (AML). Two distinct groups of FLT3 mutations are found: internal tandem duplications (ITDs) of the juxtamembrane region and point mutations within the tyrosine kinase domain (TKD). Re...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-11-4430
更新日期:2005-06-15 00:00:00
abstract::This article reports a Glanzmann thrombasthenia (GT) patient, N.M., with a point mutation in the third cysteine-rich repeat of beta3-integrin or platelet glycoprotein (GP) IIIa, leading to the expression of a constitutively activated fibrinogen receptor. The diagnosis of GT was based on a severely reduced platelet-agg...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.8.2432
更新日期:2001-10-15 00:00:00
abstract::Bruton tyrosine kinase (Btk) has a well-defined role in B-cell development, whereas its expression in osteoclasts (OCs) further suggests a role in osteoclastogenesis. Here we investigated effects of PCI-32765, an oral and selective Btk inhibitor, on osteoclastogenesis as well as on multiple myeloma (MM) growth within ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-12-396853
更新日期:2012-08-30 00:00:00
abstract::Intravenous immunoglobulin (IVIgG) causes an acute rise in the platelet count in the majority of patients with chronic immune thrombocytopenic purpura (ITP) but the mechanism(s) of action is still unknown. We evaluated the ability of three different IVIgG preparations to inhibit the in vitro binding of autoantibody to...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-11-15 00:00:00
abstract::Vascular calcification is a marker of increased cardiovascular risk. Vitamin K-dependent matrix Gla protein (MGP) is important in inhibiting calcification. Because MGP activation is vitamin K dependent, we performed a cross-sectional study investigating the relationship between the use of vitamin K antagonists and ext...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-01-264598
更新日期:2010-06-17 00:00:00
abstract::The possibility of primitive hematopoietic cell ex vivo expansion is of interest for both gene therapy and transplantation applications. The engraftment of autologous rhesus peripheral blood (PB) progenitors expanded 10 to 14 days were tracked in vivo using genetic marking. Stem cell factor (SCF)/granulocyte colony-st...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-08-15 00:00:00
abstract::Important predictors of adverse outcomes of thrombosis in children, including postthrombotic syndrome (PTS), have recently been identified. Given this knowledge and the encouraging preliminary pediatric experience with systemic thrombolysis, we sought to retrospectively analyze our institutional experience with a thro...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-12-061234
更新日期:2007-07-01 00:00:00
abstract::Despite many studies on the pathophysiology of antiphospholipid antibodies (aPL), the mechanism by which aPL causes thrombosis has not been established. We have tried to elucidate the paradox between the prolongation of the clotting time of phospholipid-dependent coagulation tests in vitro and the occurrence of thromb...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-05-15 00:00:00
abstract::Over the years, methods of cytogenetic analysis evolved and became part of routine laboratory testing, providing valuable diagnostic and prognostic information in hematologic disorders. Karyotypic aberrations contribute to the understanding of the molecular pathogenesis of disease and thereby to rational application o...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2008-02-130435
更新日期:2008-08-15 00:00:00
abstract::The Aalpha-fibrinogen Thr312Ala polymorphism, which occurs in a region involved in factor XIII (FXIII)-dependent cross-linking processes, is associated with poststroke mortality in subjects with atrial fibrillation, suggesting an influence either on intraatrial clot formation or embolization. We have determined the as...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::For most children who relapse with acute lymphoblastic leukemia (ALL), the prognosis is poor, and there is a need for novel therapies to improve outcome. We screened samples from children with B-lineage ALL entered into the ALL-REZ BFM 2002 clinical trial (www.clinicaltrials.gov, #NCT00114348) for somatic mutations ac...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-04-531871
更新日期:2014-11-27 00:00:00
abstract::Recently, we have identified scavenger receptor class A member I (SR-AI) as a receptor for coagulation factor X (FX), mediating the formation of an FX reservoir at the macrophage surface. Here, we demonstrate that the FX/SR-AI-complex comprises a third protein, pentraxin-2 (PTX2). The presence of PTX2 is essential to ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-06-724351
更新日期:2017-04-27 00:00:00
abstract::We evaluated spleen function in 193 children with sickle cell anemia 8 to 18 months of age by (99m)Tc sulfur-colloid liver-spleen scan and correlated results with clinical and laboratory parameters, including 2 splenic biomarkers: pitted cell counts (PIT) and quantitative Howell-Jolly bodies (HJB) enumerated by flow c...
journal_title:Blood
pub_type: 临床试验,杂志文章
doi:10.1182/blood-2010-04-278747
更新日期:2011-03-03 00:00:00
abstract::Thrombopoietin (Tpo) has proliferative and maturational effects on immature and more committed cells, respectively. We previously reported a role for Tpo as a survival factor in the factor-dependent human cell line M07e by demonstrating that Tpo suppresses apoptosis in the absence of induced proliferation. Wild-type p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::Heme oxygenase-1 is an essential enzyme in heme catabolism, and its human gene promoter contains a putative heat shock element (HHO-HSE). This study was designed to analyze the regulation of human heme oxygenase-1 gene expression under thermal stress. The amounts of heme oxygenase-1 protein were not increased by heat ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-06-15 00:00:00
abstract::We have designed two subfamilies of lipophilic iron (III) chelators previously termed reversed siderophores (RSFs). The agents display physicochemical properties that favor extraction of iron beyond membrane barriers of Plasmodium falciparum-infected red blood cells. We studied the in vitro antimalarial potency of RSF...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The paradoxical correlation between thrombosis and the lupus anticoagulant (LAC) effect is an enigmatic feature of the antiphospholipid (aPL) syndrome. The Dutch authors previously reported that thrombosis-related anti-beta2-glycoprotein I (beta2GPI) antibodies recognize domain I and cause LAC. The American authors re...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-07-030148
更新日期:2007-02-15 00:00:00
abstract::Lipids extracted with chloroform-methanol from red blood cell membranes of 7 PNH and 13 control subjects were used for the preparation of liposomes, which were then examined with the reactive lysis test. PNH liposomes lysed to a higher extent than control liposomes as indicated by the higher dilution of the limiting c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::Hereditary hemochromatosis is caused by mutations in the hereditary hemochromatosis protein (HFE), transferrin-receptor 2 (TfR2), hemojuvelin, hepcidin, or ferroportin genes. Hepcidin is a key iron regulator, which is secreted by the liver, and decreases serum iron levels by causing the down-regulation of the iron tra...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-09-245209
更新日期:2010-04-22 00:00:00
abstract::The importance of the second messengers calcium (Ca(2+)) and diacylglycerol (DAG) in platelet signal transduction was established more than 30 years ago. Whereas protein kinase C (PKC) family members were discovered as the targets of DAG, little is known about the molecular identity of the main Ca(2+) sensor(s). We he...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-04-218768
更新日期:2009-09-17 00:00:00