Abstract:
:Heme oxygenase-1 is an essential enzyme in heme catabolism, and its human gene promoter contains a putative heat shock element (HHO-HSE). This study was designed to analyze the regulation of human heme oxygenase-1 gene expression under thermal stress. The amounts of heme oxygenase-1 protein were not increased by heat shock (incubation at 42 degrees C) in human alveolar macrophages and in a human erythroblastic cell line, YN-1-0-A, whereas heat shock protein 70 (HSP70) was noticeably induced. However, heat shock factor does bind in vitro to HHO-HSE and the synthetic HHO-HSE by itself is sufficient to confer the increase in the transient expression of a reporter gene upon heat shock. The deletion of the sequence, located downstream from HHO-HSE, resulted in the activation of a reporter gene by heat shock. These results suggest that HHO-HSE is potentially functional but is repressed in vivo. Interestingly, heat shock abolished the remarkable increase in the levels of heme oxygenase-1 mRNA in YN-1-0-A cells treated with hemin or cadmium, in which HSP70 mRNA was noticeably induced. Furthermore, transient expression assays showed that heat shock inhibits the cadmium-mediated activation of the heme oxygenase-1 promoter, whereas the HSP70 gene promoter was activated upon heat shock. Such regulation of heme oxygenase-1 under thermal stress may be of physiologic significance in erythroid cells.
journal_name
Bloodjournal_title
Bloodauthors
Okinaga S,Takahashi K,Takeda K,Yoshizawa M,Fujita H,Sasaki H,Shibahara Ssubject
Has Abstractpub_date
1996-06-15 00:00:00pages
5074-84issue
12eissn
0006-4971issn
1528-0020journal_volume
87pub_type
杂志文章相关文献
BLOOD文献大全abstract::We evaluated the potential role of photoradiation therapy with a benzoporphyrin derivative, monoacid ring A (BPD-MA), and dihematoporphyrin ether (DHE), for the ex vivo purging of residual tumor cells from autologous bone marrow (BM) grafts. BPD-MA and DHE photosensitizing activity was tested against two human large-c...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Iron is a transition metal whose physicochemical properties make it the focus of vital biologic processes in virtually all living organisms. Among numerous roles, iron is essential for oxygen transport, cellular respiration, and DNA synthesis. Paradoxically, the same characteristics that biochemistry exploits make iro...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-01-068148
更新日期:2007-07-01 00:00:00
abstract::Patients aged ≥60 years with treatment-naive Hodgkin lymphoma (HL) have few treatment options and inferior survival due to treatment-related toxicities and comorbidities. This phase 2, nonrandomized, open-label study evaluated brentuximab vedotin (BV) monotherapy (results previously reported), BV plus dacarbazine (DTI...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2017-06-787200
更新日期:2017-12-28 00:00:00
abstract::Proapoptotic Bax and Bak are the key B-cell lymphoma-2 family members mediating apoptosis through the intrinsic pathway. Cells doubly deficient for Bax and Bak are profoundly resistant to apoptotic stimuli originating from multiple stimuli. Here we describe mice in which Bax and Bak have been deleted specifically in T...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-04-279687
更新日期:2010-12-09 00:00:00
abstract::The Philadelphia (Ph1) chromosome is present in greater than 90% of patients with chronic myelogenous leukemia (CML) and in 2% to 20% of those with acute leukemias, for which it is an important prognostic marker too. The chimeric BCR-ABL mRNAs resulting from the translocation encode either a 210-Kd or a 190-Kd protein...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-01-15 00:00:00
abstract::Granulocytic colonies grown in culture from marrow and peripheral blood from five patients with Ph1-positive CML and heterozygous at the G-6-PD locus were analyzed for G-6-PD in order to identify CFU-C that do not arise from the CML clone. The patients had both B and A enzymes in normal tissues, but their CML clones t...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1979-02-01 00:00:00
abstract::Interleukin-10 (IL-10) selectively inhibited lipopolysaccharide (LPS)-induced chemoattractant cytokine gene expression: levels of IP-10 mRNA were markedly suppressed in IL-10-treated mouse peritoneal macrophages, whereas the expression of the RANTES mRNA was only modestly reduced. IL-10 inhibited IP-10 mRNA accumulati...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-12-15 00:00:00
abstract::Immune thrombocytopenia (ITP) is an autoimmune bleeding disorder with a complex pathogenesis, which includes both antibody- and T-cell-mediated effector mechanisms. Rituximab (an anti-human CD20 monoclonal antibody [mAb]) is one of the treatments for ITP and is known to deplete B cells but may also work by affecting t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-06-655126
更新日期:2016-02-11 00:00:00
abstract::The evolutionarily conserved immune system of the zebrafish (Danio rerio), in combination with its genetic tractability, position it as an excellent model system in which to elucidate the origin and function of vertebrate immune cells. We recently reported the existence of antigen-presenting mononuclear phagocytes in ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-11-321448
更新日期:2011-06-30 00:00:00
abstract::The endothelial cell protein C receptor (EPCR) augments protein C activation by the thrombin-thrombomodulin complex. Deletion of the EPCR gene (Procr) in mice leads to embryonic lethality before embryonic day 10 (E10.0). EPCR is detected in the giant trophoblast cells at the feto-maternal boundary from E7.5 and weakly...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-01-0406
更新日期:2005-10-15 00:00:00
abstract::Circulating IgG autoantibodies that produce cytoplasmic immunofluorescence staining of ethanol-fixed normal neutrophils have recently been found in a large percentage of patients with active Wegener's granulomatosis. Such autoantibodies are rarely found in other diseases and are therefore virtually diagnostic of Wegen...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1989-11-01 00:00:00
abstract::Cells derived from human cord blood were recently used instead of bone marrow (BM) for transplantation. However, several questions concerning the potential of these cells to reconstitute the hematopoietic and immunologic system of the recipient and to induce a graft-versus-host disease (GVHD) remain unanswered. Here w...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::Von Willebrand factor (VWF) is a key hemostatic protein synthesized in both endothelial cells and megakaryocytes. Megakaryocyte-derived VWF is stored in α-granules of platelets and is enriched in hyperactive "ultra-large" VWF multimers. To elucidate the specific contribution of platelet VWF in hemostasis and thrombosi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-03-632901
更新日期:2015-10-01 00:00:00
abstract::In this issue of Blood, Rollin et al demonstrate that heparin-induced thrombocytopenia and thrombosis (HIT) patients homozygous for arginine (R) at position 131 in the immunoglobulin (Ig)G receptor FcγRIIA have a significantly higher incidence of thrombosis, and show that plasma IgG may play a key role in modulating t...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2015-02-629782
更新日期:2015-04-09 00:00:00
abstract::Plasma hyperviscosity is a rare complication of both monoclonal and polyclonal disorders associated with elevation of immunoglobulins. Asymptomatic patients with an elevation in the serum viscosity do not require plasma exchange, and the majority will have other indications for therapeutic intervention. For patients w...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2018-06-846816
更新日期:2018-09-27 00:00:00
abstract::Cytarabine (ara-C) requires activation into its triphosphorylated form, ara-CTP, to exert cytotoxic activity. Cytoplasmic 5'-nucleotidase (5NT) dephosphorylates ara-CMP, a key intermediate, preventing accumulation of ara-CTP and may reduce cellular sensitivity to the cytotoxic activity of ara-C. To determine whether t...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.6.1922
更新日期:2001-09-15 00:00:00
abstract::To increase transgenic production of granulocyte-macrophage colony-stimulating factor (GM-CSF), we mutated the mRNA's 3'-untranslated region, AUUUA instability elements. Expression vectors containing human or murine GM-CSF cDNAs coding for wild-type (GM-AUUUA) or mutant versions with reiterated AUGUA repeats (GM-AUGUA...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Alteration of lineage-specific transcriptional programs for hematopoiesis causes differentiation block and promotes leukemia development. Here, we show that AML1/ETO, the most common translocation fusion product in acute myeloid leukemia (AML), counteracts the activity of retinoic acid (RA), a transcriptional regulato...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-09-045781
更新日期:2007-05-15 00:00:00
abstract:UNLABELLED:Better methods to characterize normal human hematopoietic cells with short-term repopulating activity cells (STRCs) are needed to facilitate improving recovery rates in transplanted patients.We now show that 5-fold more human myeloid cells are produced in sublethally irradiated NOD/SCID-IL-2Receptor-γchain-n...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-09-456566
更新日期:2013-01-31 00:00:00
abstract::Partially purified hemoglobin A2 has been examined for the existence of glycosylated components by isoelectric focusing and by acid agar gel electrophoresis. Bands analogous to the glycohemoglobin derivatives of hemoglobin A, hemoglobin-A1.a.b.c, were readily detected. Evidence that these minor bands are in fact glyco...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::The most frequently recurring translocations in mucosa-associated lymphoid tissue (MALT) B-cell non-Hodgkin lymphoma, t(11;18)(q21;q21) and t(14;18)(q32; q21), lead to formation of an API2-MALT1 fusion or IgH-mediated MALT1 overexpression. Various approaches have implicated these proteins in nuclear factor kappaB (NF-...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-06-2297
更新日期:2005-04-01 00:00:00
abstract::Since the National Institutes of Health Chronic Graft-Versus-Host Disease (cGVHD) Consensus Project in 2005, a need has emerged to evaluate cGVHD more methodically, not only to make a cGVHD diagnosis, but also to accurately classify individual organ and global organ severity, at baseline and in follow-up so that subje...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-314815
更新日期:2011-09-08 00:00:00
abstract::In this issue of Blood, Rieger et al show that malaria parasite infiltration in the human placenta requires a specific geometry and affinity of host receptors to facilitate strong adhesion. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2014-11-610634
更新日期:2015-01-08 00:00:00
abstract::The JAK2 V617F mutation is present in the majority of patients with a myeloproliferative neoplasm (MPN) and is sufficient to recapitulate an MPN in murine models. However, the consequences of JAK2 mutations for myeloid differentiation are poorly understood. After systematic analyses of a large cohort of JAK2-mutated M...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-12-327593
更新日期:2011-07-07 00:00:00
abstract::Lymphomatoid granulomatosis (LyG) is an angiodestructive lymphoproliferative disorder (LPD) often involving the lungs. Its etiology is uncertain, but a number of previous studies had suggested it is a T-cell LPD associated with Epstein-Barr virus (EBV). Because of the similarity between LYG and nasal angiocentric lymp...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-06-01 00:00:00
abstract::Oxygen-induced retinopathy (OIR) is a well-characterized model for retinopathy of prematurity, a disorder that results from rapid microvascular proliferation after exposure of the retina to high oxygen levels. Here, we report that the proliferative phase of OIR requires transcriptional induction of the annexin A2 (A2)...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-03-341214
更新日期:2011-09-08 00:00:00
abstract::We prepared a heterohybrid cell line that secretes a human IgM monoclonal autoantibody that recognizes an antigen found on thrombin-activated or stored platelets. The surface expression of the epitope recognized by this autoantibody, 5E5, increases with time as platelets age in vitro, suggesting that it may represent ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::The granulocyte-macrophage colony-stimulating factor (GM-CSF)/interleukin (IL)-3/IL-5 receptor family regulates the production and function of myeloid cells. These cytokines signal through receptor complexes that consist of unique ligand-binding alpha-chains and common signaling beta-chains. IL-5 is distinct from IL-3...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-03-208850
更新日期:2009-10-29 00:00:00
abstract::Deletions at the 3' end of the human beta-globin locus are associated with the hereditary persistence of fetal hemoglobin (HPFH) in adults, potentially through the juxtaposition of enhancer elements in the vicinity of the fetal gamma-globin genes. We have tested how sequences at the HPFH-2, HPFH-3, and HPFH-6 breakpoi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-05-1681
更新日期:2003-11-01 00:00:00
abstract::The effects of interleukin (IL)-8 on spontaneous IgE and IgG4 production in atopic patients were studied. IL-8 inhibited IgE and IgG4 production by purified surface (s) IgE+ and sIgG4+ B cells, respectively, while it had no effect on IgG1, IgG2, IgG3, IgM, IgA1, and IgA2 production by corresponding sIg+ B cells. The I...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00