Abstract：
BACKGROUND AND OBJECTIVES:Q-switched lasers had been used in the treatment of lentigo maligna but their role remains controversial. While previous studies have addressed the change in adhesion molecule expression after sub-lethal laser damage, no study has addressed the impact of sub-lethal laser damage at a molecular level. The p16 gene has been proposed as the candidate gene for melanoma. Our objective is to examine the effect of sub-lethal laser damage on p16 expression in melanoma cell lines. STUDY DESIGN/MATERIALS AND METHODS:Three human melanoma cell lines-HTB 66, Sk-mel-24 (HTB 71), and G361-were irradiated by a Q-switched 755 nm Alexandrite laser at fluencies that ranged from 0.85 to 2.0 J/cm(2). HTB 66 was the only cell line with significant expression of p16INK4a while the other two cells lines were p16INK4a negative and served as negative control. Protein and mRNA expression for p16 were assessed by flow cytometry and RT-PCR, respectively. RESULTS:The level of p16INK4a protein in cell line HTB 66 increased significantly after laser irradiation as compared with non-irradiated cells. The level of p16INK4a protein did not change in p16INK4a-negative cell lines (Sk-mel-24 and G361). However, there was only a slight increase in the percentage of G0/G1 phase cells. CONCLUSIONS:Sub-lethal laser damage could increase DNA damage leading to an increase in p16 expression, and such effect would be particularly undesirable for patients with p16 mutation. Further studies are warranted to examine the role of sub-lethal laser damage in inducing p16 mutation.
journal_name
Lasers Surg Medjournal_title
Lasers in surgery and medicineauthors
Chan HH,Xiang L,Leung JC,Tsang KW,Lai KNdoi
10.1002/lsm.10118subject
Has Abstractpub_date
2003-01-01 00:00:00pages
88-93issue
2eissn
0196-8092issn
1096-9101journal_volume
32pub_type
杂志文章abstract：BACKGROUND AND OBJECTIVE:Endoscopic third ventriculostomy is used to treat hydrocephalus. Different laser wavelengths have been proposed for laser-assisted endoscopic third ventriculostomies over the last decades. The aim of this study was to evaluate Thulium laser endoscopic third ventriculostomy heat penetration in t...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.22779
更新日期：2017-12-07 00:00:00
abstract：:Laser balloon angioplasty with Nd:YAG energy has been proposed as a method to seal intimal dissection and prevent elastic recoil after balloon angioplasty. To better define the vessel response to laser balloon angioplasty, its effects on luminal diameter, Indium-111 labelled platelet deposition, and histology were stu...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900140105
更新日期：1994-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Hyperhidrosis is a condition that affects a large percentage of the population and has a significant impact on peoples' lives. This report presents a technical overview of a new noninvasive, microwave-based device for creating thermolysis of sweat glands. The fundamental principles of operatio...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.21142
更新日期：2012-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:To functionally evaluate the efficacy of glatiramer acetate (Cop-1) as a neuroprotective treatment for laser-induced retinal injuries in rats. STUDY DESIGN/MATERIALS AND METHODS:Using standard lasering and flash ERG techniques, we evaluated the effect of photocoagulation and of Cop-1 treatmen...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.20610
更新日期：2008-03-01 00:00:00
abstract：BACKGROUND:Patients suffering from malignant gliomas have a poor prognosis. For the surgical treatment of these tumors, 5-aminolevulinic acid (5-ALA) has become a new standard. AIMS:This review intends to provide an overview over current status, significance, limitations, and future perspectives of 5-ALA based fluores...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章,评审
doi：10.1002/lsm.22933
更新日期：2018-07-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The aim was to investigate the bactericidal effect of the 1,064 nm Nd:YAG laser on Staphylococcus epidermidis. MATERIAL AND METHODS:S. epidermidis was inoculated on agar plates and then exposed to pulsed laser light in three different modes: with an uninterrupted train of pulses, or with two d...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/1096-9101(2000)27:4<336::aid-lsm6>3.0.co;2
更新日期：2000-01-01 00:00:00
abstract：:For our study of the effect of low energy laser irradiation (LELI) on living tissue we used HeNe laser on rats. The exponential absorption was reaffirmed in the living tissues overlying the sciatic nerve. An optimal range of energy between 3.5 and 7 J--associated with energy concentration of 4-10 J/cm2 delivered trans...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900060502
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The aim of this study was to evaluate the efficacy and safety of the intense pulsed light source (IPL) and the Nd:YAG laser in the treatment of facial rhytids. Both systems can be used in a non-ablative manner and cause a dermal wound. This is thought to stimulate the production of new collagen...
journal_title：Lasers in surgery and medicine
pub_type： 临床试验,杂志文章
doi：10.1002/lsm.1029
更新日期：2001-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Sutures are currently the gold standard for wound closure but they are still unable to seal tissue and may induce scarring or inflammation. Biocompatible glues, based on polysaccharides such as chitosan, are a possible alternative to conventional wound closure. In this study, the adhesion of l...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.20418
更新日期：2007-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:In endodontics, a major diagnostic challenge is the accurate assessment of pulp status. In this study, we designed and characterized a fiber-based laser speckle imaging system to study pulsatile blood flow in the tooth. STUDY DESIGN/MATERIALS AND METHODS:To take transilluminated laser speckle ...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.22370
更新日期：2015-08-01 00:00:00
abstract：:During a ten-year period 642 abdominal hollow organ resections have been performed by means of a new, original technique--that is, CO2 laser irradiation combined with specially designed instrumentation. This resulted in a remarkable decrease in postoperative morbidity and mortality. The article describes the laser pro...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900070402
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:To further understand the precise process of the tumor cell degeneration after photodynamic therapy (PDT), laser hyperthermic therapy (LH), and combined treatments using an Nd:YAG laser. It is important to examine initial morphological alteration of tumor cells after these treatments. STUDY DE...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900160308
更新日期：1995-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:In this study, methylene blue-mediated photodynamic therapy (MB-PDT) was used as a possible alternative method for the treatment of oral lichen planus (OLP). STUDY DESIGN/MATERIALS AND METHODS:Thirteen patients with 26 OLP lesions were enrolled in this study. Patients were instructed to gargl...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.20278
更新日期：2006-01-01 00:00:00
abstract：:This study examined both the effect of variations in optical fiber tip and in light wavelength on laser-induced hyperthermia in rat brain. Normal rat brains were exposed to argon laser light (454-514.5 nm) delivered through an intracerebral end-emitting (bare-tipped) or a diffusion-emitting (sapphire-tipped) optical f...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900070413
更新日期：1987-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Lasers can be used to modify the chemical composition of dental enamel to increase the bond strength to restorative materials and to render the mineral phase more resistant to acid dissolution. Previous studies have suggested a synergistic relationship between CO(2) laser irradiation and fluor...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.10162
更新日期：2003-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The extent of thermal injury during laser prostatectomy is dependent on the light distribution in laser-irradiated tissue. As tissue is irradiated, the optical properties change as a function of temperature due to an alteration of molecular and cellular structure. The purpose of the present stu...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/(sici)1096-9101(1999)24:1<38::aid-lsm7>3.0
更新日期：1999-01-01 00:00:00
abstract：:Diagnostic endoscopy has generally been done under intravenous sedation. Diazepam and meperidine are the two most frequent drugs used. Even though these drugs produce sedation, analgesia, and amnesia, they do not provide a quiet field. Therapeutic laser endoscopy requires a quiet field. We conclude that an anesthesiol...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900070311
更新日期：1987-01-01 00:00:00
abstract：:This study was designed to examine the acute response of normal arterial wall to pulsed laser irradiation. Irradiation with an Excimer or a Holmium YAG laser was performed in 15 normal iliac sites of 8 male New Zealand white rabbits. The excimer laser was operated at 308 nm, 25 Hz, 50 mJ/mm2/pulse, and 135 nsec/pulse ...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900130404
更新日期：1993-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:A port wine stain (PWS) is a type of capillary vascular malformation composed of malformed, dilated blood vessels within the papillary and reticular dermis. Currently, pulsed dye laser (PDL) is considered the therapeutic gold standard, although greater than 90% of lesions may be refractory to t...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.22436
更新日期：2016-02-01 00:00:00
abstract：:Using an optically shielded fiber optic laser catheter, the amount of gas produced when firing an argon ion laser into 0.9% saline solution or blood alone and into atheromatous aorta in either a blood or 0.9% saline medium was quantitated. Energies from 0.25 to 4 joules (J) were used at powers of 2, 5, and 8 W. We fou...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900080113
更新日期：1988-01-01 00:00:00
abstract：:We used a Q-switched Nd:YAG laser with non-linear optical crystals to produce the 5th (213 nm) and the 4th (266 nm) harmonic frequencies. Using these two wavelengths, we ablated fresh porcine corneas and type I collagen synthetic epikeratoplasty lenticules. For the 213-nm ablation, radiant exposure was 1.3 J/cm2. The ...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900110610
更新日期：1991-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Attempts at using the pulsed dye laser (PDL) operating at 585 nm wavelength and 0.45 milliseconds pulse duration to treat leg veins have been notably unsuccessful. The aim of this study is to evaluate the efficacy and complications of the 595 nm long PDL in the treatment of leg veins. STUDY D...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.20035
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Soft tissue sarcomas (STS) are a rare and heterogeneous group of malignant tumors that are often treated through surgical resection. Current intraoperative margin assessment methods are limited and highlight the need for an improved approach with respect to time and specificity. Here we investi...
journal_title：Lasers in surgery and medicine
pub_type： 临床试验,杂志文章
doi：10.1002/lsm.22564
更新日期：2016-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:The nonablative application of holmium:yttrium-aluminum-garnet (Ho:YAG) laser energy to the joint capsule of patients with glenohumeral instability has been found to shrink capsular tissue and to help stabilize the joint. The purpose of this study was to evaluate the effect of nonablative laser...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/(sici)1096-9101(1997)20:2<164::aid-lsm7>3.
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND:Low-level laser therapy (LLLT) is a noninvasive treatment for a wide-assortment of medical ailments. A recent application is for noninvasive body slimming. A Level 1 clinical study was completed and recorded a significant reduction in circumferential measurements across waist, hips, and thighs compared to pl...
journal_title：Lasers in surgery and medicine
pub_type： 临床试验,杂志文章
doi：10.1002/lsm.22007
更新日期：2012-03-01 00:00:00
abstract：:Ablative lasers (CO2 and Er:YAG) provide the greatest improvement in photoaging, but significant adverse effects limit their use. Nonablative lasers have reduced adverse effects, but limited efficacy. Fractional photothermolysis (FP) produces arrays of microscopic thermal wounds called microscopic treatment zones (MTZ...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.20310
更新日期：2006-03-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Herein an overview is provided of the causes, consequences, and significance of photodynamic therapy (PDT)-mediated effects on tumor oxygenation and blood flow during illumination. STUDY DESIGN/MATERIALS AND METHODS:Techniques particularly valuable to this research have included tissue oxygen ...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章,评审
doi：10.1002/lsm.20355
更新日期：2006-06-01 00:00:00
abstract：BACKGROUND AND OBJECTIVES:Laser-induced photo thermal damage has been combined with photodynamic therapy (PDT) using a systemic photosensitiser to treat vascular lesions. The efficacy of PDT using systemic 5-aminolaevulinic acid (5-ALA) as the photosensitiser and pulsed dye laser (PDL) as the light source in port wine ...
journal_title：Lasers in surgery and medicine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1002/lsm.20151
更新日期：2005-04-01 00:00:00
abstract：:The use of lasers as surgical tools may open up new possibilities for the treatment of atherosclerotic occlusive vascular disease. However, basic information regarding the effect of laser radiation to the lumen of normal blood vessels is needed prior to clinical application. This work investigates the nature of the CO...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章
doi：10.1002/lsm.1900030308
更新日期：1983-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Very few treatments for striae are based on prospective randomized trials. The objective of this study was to assess the efficacy of bipolar fractional radiofrequency and bipolar radiofrequency potentiated with infrared light, alone or combined, for treating abdominal stretch marks. STUDY DESI...
journal_title：Lasers in surgery and medicine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1002/lsm.22458
更新日期：2016-03-01 00:00:00
