Abstract：
:DNA-dependent RNA polymerase A (or 1) was purified from murine myeloma MOPC 21 by diethylaminoethyl Sephadex chromatography. Further separation from DNA polymerases, protein kinase and DNA endonuclease was accomplished by polyriboadenylate-Sepharose affinity chromatography followed by gradient centrifugation. Yields following chromatography were 100%, but following gradient centrifugation only 25 to 30% of the activity remained. Addition of low-molecular-weight components increased yields to 50 to 60%. Several species of myeloma polymerase A could be detected, and subunits of 190,000 and 125,000 daltons were identified. No evidence of phosphorylation of the polymerase was found.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Hall SH,Smuckler EAsubject
Has Abstractpub_date
1976-03-01 00:00:00pages
1094-1100issue
3eissn
0008-5472issn
1538-7445journal_volume
36pub_type
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