Abstract:
:Clinical trials of gene therapy for cystic fibrosis suggest that current levels of gene transfer efficiency are probably too low to result in clinical benefit, largely as a result of the barriers faced by gene transfer vectors within the airways. The respiratory epithelium has evolved a complex series of extracellular barriers (mucus, lack of receptors, immune surveillance, etc.) aimed at preventing penetration of lumenally delivered materials, including gene therapy vectors. In addition, once in the cell, further hurdles have to be overcome, including DNA degradation, nuclear import and the ability to maintain long-term transgene expression. Strategies to overcome these barriers will be addressed in this review and include the use of: (i) clinically relevant adjuncts to overcome the extra- and intracellular barriers; (ii) less-conventional delivery routes, such as intravenous or in utero administration; (iii) more efficient non-viral vectors and 'stealth' viruses which can be re-administered; and (iv) new approaches to prolong transgene expression by means of alternative promoters or integrating vectors. These advances have the potential to improve the efficiency of gene delivery to the airway epithelium, thus making gene therapy a more realistic option for cystic fibrosis.
journal_name
Adv Drug Deliv Revjournal_title
Advanced drug delivery reviewsauthors
Ferrari S,Geddes DM,Alton EWdoi
10.1016/s0169-409x(02)00145-xsubject
Has Abstractpub_date
2002-12-05 00:00:00pages
1373-93issue
11eissn
0169-409Xissn
1872-8294pii
S0169409X0200145Xjournal_volume
54pub_type
杂志文章,评审abstract::miRNA-based technologies provide attractive tools for several liver tissue engineering approaches. Herein, we review the current state of miRNA applications in liver tissue engineering. Several miRNAs have been implicated in hepatic disease and proper hepatocyte function. However, the clinical translation of these fin...
journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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更新日期:2012-05-01 00:00:00
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
doi:10.1016/j.addr.2012.03.013
更新日期:2012-09-01 00:00:00
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
doi:10.1016/s0169-409x(03)00029-2
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abstract::One of the most common causes of illnesses in humans is from respiratory tract infections caused by bacterial, viral or fungal pathogens. Inhaled anti-infective drugs are crucial for the prophylaxis and treatment of respiratory tract infections. The benefit of anti-infective drug delivery via inhalation is that it aff...
journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
doi:10.1016/j.addr.2014.11.004
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
doi:10.1016/j.addr.2004.09.009
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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journal_title:Advanced drug delivery reviews
pub_type: 杂志文章
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journal_title:Advanced drug delivery reviews
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journal_title:Advanced drug delivery reviews
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abstract::Lanthanide-doped upconverting luminescent nanoparticles (UCNPs) are promising materials for optical imaging-guided drug delivery and therapy due to their unique optical and chemical properties. UCNPs absorb low energy near-infrared (NIR) light and emit high-energy shorter wavelength photons. Their special features all...
journal_title:Advanced drug delivery reviews
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abstract::Lack of approved pharmaceutical agents and/or pharmacokinetic data in the literature for exotic, wildlife, and zoo species is a major issue for veterinarians. These practitioners must take approved agents (veterinary or human) and extrapolate their use to non-approved species with little or no scientific basis to supp...
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abstract::Adult neural stem and progenitor cells (NSPCs) offer a unique opportunity for neural regeneration and niche modification in physiopathological conditions, harnessing the capability to modify from neuronal circuits to glial scar. Findings exposing the vast plasticity and potential of NSPCs have accumulated over the pas...
journal_title:Advanced drug delivery reviews
pub_type: 杂志文章,评审
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