Abstract：
:An efficient production method of heme-iron-enriched peptide was developed based on enzymatic hydrolysis. Hemoglobin hydrolysis, carried out stepwise with commercially available exopeptidase and endopeptidase, resulted in an increased degree of hydrolysis (DH). Exopeptidase-catalyzed protein hydrolysis formed low molecular weight peptides and amino acids. Different process parameters including dialysis and ultra- and diafiltration were evaluated. Heme/peptide ratio increased as molecular weight cut-off (MWCO) of the dialysis membrane increased. When the hydrolysate was dialyzed against sodium phosphate buffer, a higher heme/ peptide ratio was obtained. The heme/peptide ratio of the hydrolysate reached up to 25.4% when the dialysis was carried out with a membrane of 12-14 kDa MWCO. Also, the ratio was improved by the use of ultrafiltration and diafiltration on the pilot-scale.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
In MJ,Chae HJ,Oh NSdoi
10.1016/s0960-8524(02)00009-3subject
Has Abstractpub_date
2002-08-01 00:00:00pages
63-8issue
1eissn
0960-8524issn
1873-2976pii
S0960-8524(02)00009-3journal_volume
84pub_type
杂志文章abstract：:Column studies were conducted, using iron oxide-coated Aspergillus niger biomass, to examine the removal of arsenic [As(III) and As(V)] from an aqueous solution. The Thomas and Yan models were examined to predict the breakthrough curves. The Yan Model described the data better (based on the R(2) values) when compared ...
journal_title：Bioresource technology
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