Abstract：
:The metabolizing potential of a bacterial strain Rhodococcus MTCC 6716, isolated from the gut of an Indian earthworm (Metaphire posthuma) was studied for endosulfan bioremediation. In the present work, the optimum conditions for the maximum growth, kinetic of endosulfan degradation, regression equation, half life and correlation coefficient were studied. Endosulfan induced alterations in the expression of mRNA and protein of specific endosulfan metabolizing marker gene (Esd) was studied. Maximum growth of bacteria was observed at pH 7.0, 30°C and 0.085 M sodium chloride concentration in a liquid culture medium. Endosulfan was degraded by Rhodococcus strain up to 97.23% within 15 days without producing toxic metabolite and with strong correlation coefficient (-0.728) and half life 5.99 days. Endosulfan degradation was mediated through gene(s) present in genomic DNA. Expression of marker gene was found endosulfan concentration dependent. The results suggest that this novel strain (Rhodococcus) may be utilized for bioremediation of endosulfan.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Verma A,Ali D,Farooq M,Pant AB,Ray RS,Hans RKdoi
10.1016/j.biortech.2010.10.005subject
Has Abstractpub_date
2011-02-01 00:00:00pages
2979-84issue
3eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01653-6journal_volume
102pub_type
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