Abstract：
:A high throughput enzyme assay platform, called GENPLAT, was used to guide the development of an optimized mixture of individual purified enzymes from ten "accessory" and six "core" enzymes. Enzyme mixtures were optimized for release of Glu, Xyl, or a combination of the two from corn stover pretreated by ammonia-fiber expansion (AFEX). Assay conditions were a fixed enzyme loading of 15 mg/g glucan, 48 h digestion, and 50 degrees C. Five of the ten tested accessory proteins enhanced Glu or Xyl yield compared to the core set alone, and five did not. An 11-component mixture containing the core set and five accessory enzymes optimized for Glu released 52.1% of the available Glu, compared to 38.5% with the core set alone. A mixture optimized for Xyl released 39.9% of the Xyl, compared to 26.4% with the core set alone. We predict that there is still considerable opportunity for further improvement of synthetic mixtures. Furthermore, the strategy described here is applicable to the development of more efficient enzyme cocktails for any pretreatment/biomass combination and for detecting enzymes that make a heretofore unrecognized contribution to lignocellulose deconstruction.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Banerjee G,Car S,Scott-Craig JS,Borrusch MS,Bongers M,Walton JDdoi
10.1016/j.biortech.2010.07.028subject
Has Abstractpub_date
2010-12-01 00:00:00pages
9097-105issue
23eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01216-2journal_volume
101pub_type
杂志文章abstract：:Hydroponic experiment was carried out to investigate the effect of cadmium (Cd) on growth, Cd accumulation, lipid peroxidation, antioxidative enzymes, leaf chlorophyll, root activity, protein content and Cd uptake kinetics of vetiver grass. The results showed that 1 mg/L Cd in solution led to increased chlorophyll con...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.03.028
更新日期：2010-08-01 00:00:00
abstract：:Multi-stage continuous (chemostat) culture fermentation (MCCF) with variable fermentor volumes was carried out to study the utilization of glucose and xylose for ethanol production via mixed sugar fermentation (MSF). Variable fermentor volumes were used to enable enhanced sugar utilization, accounting for differences ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2009.09.042
更新日期：2010-02-01 00:00:00
abstract：:Fe is widely used as an additive in anaerobic digestion, but its bioavailability and the mechanism by which it enhances digestion are unclear. In this study, sequential extraction was used to measure Fe bioavailability, while biochemical parameters, kinetics model and Q-PCR (fluorescence quantitative PCR) were used to...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.07.151
更新日期：2018-02-01 00:00:00
abstract：:The green microalga Haematococcus pluvialis was cultured with different concentrations of NaNO(3) to determine the effect on cell growth and astaxanthin accumulation. The optimum nitrate concentration to obtain astaxanthin and to avoid the cessation of cell division was 0.15 g/l NaNO(3). The ratio chlorophyll a/total ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2004.04.006
更新日期：2005-02-01 00:00:00
abstract：:Biogas is an environment-friendly fuel but that must be upgraded before being utilized. The method about removing CO2 from biogas by microalgal culturing using biogas effluent as nutrient medium in this study could effectively upgrade biogas and simultaneously reduce the biogas effluent nutrient. Results showed that t...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2013.04.054
更新日期：2013-07-01 00:00:00
abstract：:In this study, a novel aerated surface flow constructed wetland (SFCW) using exhaust gas from biological wastewater treatment was investigated. Compared with un-aerated SFCW, the introduction of exhaust gas into SFCW significantly improved NH4+-N, TN and COD removal efficiencies by 68.30 ± 2.06%, 24.92 ± 1.13% and 73....
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pub_type： 杂志文章
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更新日期：2018-02-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.10.143
更新日期：2015-01-01 00:00:00
abstract：:Dried cattle-manure compost was pyrolyzed by a one-step process to obtain activated carbon using chemical activation by zinc chloride. The influence of activation parameters such as ZnCl(2) to cattle-manure compost (ZnCl(2)/CMC) ratio, activation temperature and retention time on the final products was investigated. T...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.12.023
更新日期：2007-01-01 00:00:00
abstract：:The binary sorption of Pb(II) and Cu(II) onto calcium alginate, algal biomass and algal/glutaraldehyde-crosslinked polyethyleneimine (PEI) composite beads was studied in the absence and presence of Ca(II). Different competitive models were compared for predicting the equilibrium data. Results show that all the sorbent...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.01.066
更新日期：2017-05-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章,评审
doi：10.1016/j.biortech.2020.123800
更新日期：2020-10-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2016.07.121
更新日期：2016-11-01 00:00:00
abstract：:An electrochemical sensor for detection of the activity of laccase from Penicillium simplicissimum isolated from the composting has been developed. The sensor is based on glassy carbon electrode modified with multi-wall carbon nanotubes (CNTs). The introduction of CNTs into this system can greatly enhance the electroc...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2008.04.029
更新日期：2008-12-01 00:00:00
abstract：:The economic feasibility of algal mass culture for biodiesel production is enhanced by the increase in biomass productivity and storage lipids. Effect of iron on growth and lipid accumulation in marine microalgae Chlorella vulgaris were investigated. In experiment I, supplementing the growth media with chelated FeCl3 ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.09.073
更新日期：2008-07-01 00:00:00
abstract：:The purpose of this study was to investigate the effect of different types of food wastes on the process efficiency and microbial community structures in full-scale anaerobic digesters and to identify parameters that affect these criteria. Six full-scale anaerobic digesters were investigated; three were operated under...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2017.08.167
更新日期：2017-12-01 00:00:00
abstract：:In this study, three lab-scale anaerobic sulfidogenic bioreactors were operated independently using three different mixing modes (hydraulic, mechanical and pneumatic). One-way ANOVA test indicated various performance parameters (e.g. sulfate reduction and sulfide production) and granular sludge properties (e.g. EPS an...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122397
更新日期：2020-02-01 00:00:00
abstract：:This study investigated the feasibility of using pre-treated plant liquors as organic carbon sources for the treatment of hydroponic wastewater containing high nitrate-N (>300 mg N/L). The waste plant material was pre-treated to extract organic carbon-rich liquors. When this plant liquor was used as an organic carbon ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.07.009
更新日期：2008-05-01 00:00:00
abstract：:A freshwater algae Chlorella pyrenoidosa was cultured outdoors using anaerobically digested activated sludge effluent. The effects of pH variations were evaluated. The coupled pH variations and free ammonia toxicity significantly affected the algal growth, lipids accumulation and contamination control during every sea...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2015.10.095
更新日期：2016-01-01 00:00:00
abstract：:A bio-cathode microbial fuel cell (MFC) with tubular membrane was integrated to construct a microbial fuel cell-tubular membrane bioreactor (MFC-TMBR) system, in which the bio-cathode MFC was developed as a biosensor for COD real-time monitoring in TMBR and the performance was analyzed in terms of its current variatio...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.08.033
更新日期：2014-10-01 00:00:00
abstract：:A laboratory-scale flat panel photobioreactor was operated for the continuous growth of Scenedesmus obliquus and consequent removal of nutrients in wastewater. This study develops a simple model by which biomass values in continuous operation can be predicted from kinetic growth parameters obtained from a shorter batc...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2012.09.103
更新日期：2013-01-01 00:00:00
abstract：:A one-pot process combining transesterification and selective hydrogenation was established to produce biodiesel from hemp (Cannabis sativa L.) seed oil which is eliminated as a potential feedstock by a specification of iodine value (IV; 120 g I(2)/100g maximum) contained in EN 14214. A series of alkaline earth metal ...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2010.02.095
更新日期：2010-08-01 00:00:00
abstract：:The recovery of the total orthophosphate (PT) and removal of the total ammonia-nitrogen (TAN) from swine wastewater were investigated through a combined technology of using bittern as the magnesium source in struvite precipitation along with internal recycling of the chlorination product of the recovered struvite. Res...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2014.09.024
更新日期：2014-11-01 00:00:00
abstract：:In this work, effect of Fe0 nanoparticles (Fe0 NPs) on macroalgae fermentation was explored. Hydrogen production was significantly enhanced by 6.5 times comparing with control test, achieving 20.25 mL H2/g VSadded with addition of 200 mg/L Fe0 NPs. In-depth analysis of substrate conversion showed that both hydrogen ge...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.02.128
更新日期：2019-06-01 00:00:00
abstract：:Purple phototrophic bacteria (PPB) have been proposed as a high-growth, assimilative option for wastewater treatment. The original partition-release-recover concept proposal requires their near complete digestion and release (and subsequent recovery) of energy and nutrients in an anaerobic digester. While the growth (...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2020.123125
更新日期：2020-03-06 00:00:00
abstract：:Live Phormidium bigranulatum-dominated mat successfully removed Pb(II), Cu(II) and Cd(II) from aqueous solution. Percent metal removal approached equilibrium within 4h, independent of mat thickness (0.2-1.6 mm), in batch system. But % metal removal increased with increase in mat thickness due to enhancement of biomass...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2011.11.002
更新日期：2012-01-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.10.058
更新日期：2008-09-01 00:00:00
abstract：:Converting poultry feather biomass into useful products presents a new avenue of utilization of agricultural waste material. However, not much is understood about the poultry feather structure or methods to process it. In this study, formic acid vapor is systematically allowed to penetrate the feather fiber structure,...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2005.02.039
更新日期：2006-01-01 00:00:00
abstract：:The effect of different mixing ratios of fish processing waste (FPW) with a liquid fraction (LF) of hydrothermal carbonization (HTC) of bamboo residues on biogas and methane yield was investigated. The different mixing ratios (FPW + LF) and HTC temperature (200-280 °C) had significant effects on biogas and methane pro...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2019.122542
更新日期：2020-02-01 00:00:00
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journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2007.02.041
更新日期：2008-03-01 00:00:00
abstract：:To improve microalgae harvesting efficiency and to reduce the addition of chemicals in the buoy-bead flotation process, a novel buoy-bead flotation approach has been developed for harvesting Chlorella vulgaris, using surface-layered polymeric microspheres (SLPMs). Next, the detachment of microalgae cell-SLPM aggregate...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2018.07.065
更新日期：2018-11-01 00:00:00
abstract：:Substrate leaching experiments were performed to study the relative leaching potential of pendimethalin in various types of soil matrices. Pendimethalin leaching showed up to a depth of 30 cm in all the studied soil matrices, irrespective of pH conditions used. The leaching potential of pendimethalin was assessed at v...
journal_title：Bioresource technology
pub_type： 杂志文章
doi：10.1016/j.biortech.2006.09.049
更新日期：2007-11-01 00:00:00
