Abstract:
:This study examined the effects of mechanical compression on engineered cartilage in a novel hybrid culture system. Cylindrical holes were cut in discs of bovine articular cartilage and filled with agarose gels containing chondrocytes. These constructs were compressed in radiolabeled medium under static or oscillatory unconfined compression. Oscillatory compression at 1 Hz significantly stimulated synthesis above static control levels. Control experiments indicate that oscillatory compression does not stimulate freshly cast gels (without annuli), but does so after several weeks. This may be because physiologic fluid flow levels do not occur until sufficient extracellular matrix has accumulated. Finite element models predict minimal fluid flow in the gel core, and minimal differences in flow patterns between free and constrained gels. However, the models predict fluid pressures in constrained gels to be substantially higher than those in free gels. Our results suggest that pressure variations may influence synthesis of engineered cartilage matrices, with implications for construct development and post-implantation survival.
journal_name
Biorheologyjournal_title
Biorheologyauthors
Hunter CJ,Levenston MEsubject
Has Abstractpub_date
2002-01-01 00:00:00pages
79-88issue
1-2eissn
0006-355Xissn
1878-5034journal_volume
39pub_type
杂志文章相关文献
BIORHEOLOGY文献大全abstract::Through cardiac looping during embryonic development, human and other vertebrate hearts adopt sinuous curvatures with marked changes in direction of flow at atrial, ventricular and arterial levels. We used magnetic resonance phase velocity mapping to study flow through the hearts of resting volunteers, and Doppler ult...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::A rigidity index (RI) related to red blood cell deformability was measured by using the hemorheometre. The RI for 13 patients homozygous for sickle cell disease was 109 +/- 44 at 37 degrees C and at atmospheric pO2. The filtration time curve as a function of pO2 is biphasic for sickle cell suspensions. The pO2 at whic...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1989-26409
更新日期:1989-01-01 00:00:00
abstract::The present study was designed to investigate the oxidant susceptibility of red blood cells (RBC) from four species (echidna, human, koala, Tasmanian devil) based on changes in cellular deformability. These species were specifically chosen based on differences in lifestyle and/or biology associated with varied levels ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2012-0599
更新日期:2011-01-01 00:00:00
abstract::Aqueous solutions calf thymus DNA at three concentrations were studied by viscosity measurements at ambient pressure. A gravitational capillary viscometer and a rotating cylinder viscometer were employed. Three polycations were tested as viscosity-reducing agents with measurements from 25°C to 50°C. The most significa...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-140649
更新日期:2014-01-01 00:00:00
abstract::There have been few reports describing the effects of mechanical loading on the metabolism of meniscal cells. The aim of this study was to investigate the effects of hydrostatic pressure on meniscal cell metabolism. Human meniscal cells were cultured in alginate beads for 3 days. They were then subjected to 4 MPa hydr...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:Leukocytes and platelets typically fulfil their functions through adhesion to the walls of vessels with different size, haematocrit and shear rate. OBJECTIVE:We aimed to investigate differential effects of these variables on leukocyte and platelet adhesion. METHODS:Blood with varying haematocrit was perfus...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15043
更新日期:2015-01-01 00:00:00
abstract::The application of principles of biorheology, hemorheology and perihemorheology on problems of the nervous system in health and disease was suggested by Alfred L. Copley (1982, 1987). Late in 1988 Copley and Sourander considered neurobiorheology to be an appropriate term for a new branch of biorheology bridging the ga...
journal_title:Biorheology
pub_type: 历史文章,杂志文章
doi:10.3233/bir-1991-28603
更新日期:1991-01-01 00:00:00
abstract::The effects of platelet margination and enhanced platelet diffusivity, as induced by red blood cells, on the adhesion of platelets, were investigated for a range of haematocrits, under 2D axi-symmetric flow, simulating previous in vitro experiments [Microvasc. Res. 17 (1979), 238-262]. The effect of margination was in...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The thixotropic parameters of whole blood from 314 healthy subjects (154 women, 160 men) were measured with our modified method by Low shear 30 Rheometer and calculated according Huang's equation. This communication offered the reference range of thixotropic parameters from man and woman group. The results demonstrate...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-283-409
更新日期:1991-01-01 00:00:00
abstract::Adhesion of monocytes (U-937 cells) along a silicone tube deployed with circular ring stents was studied to test the hypothesis that by intentionally inducing swirling flow in an endovascular stent, the adhesion of monocytes would be suppressed. The results revealed that under a normal flow condition, the flow disturb...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-120622
更新日期:2012-01-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) deformability may increase, or decrease, following application of shear stress ("shear conditioning"), depending upon the specific magnitude and duration of exposure. However, the time course of altered RBC deformability following shear remains unresolved. OBJECTIVE:We utilised shear co...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-17151
更新日期:2018-01-01 00:00:00
abstract::The membrane shear elastic modulus (mu) and the time constant for extensional shape recovery (tc) were measured for normal, control human red blood cells (RBC) and for RBC heat treated (HT) at 48 degrees C. Three separate methods for the measurement of mu were compared (two used a micropipette and one employed a flow ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1985-22106
更新日期:1985-01-01 00:00:00
abstract::The purpose of this study was to determine the shear rate at the wall in a symmetrically branched tube with a branch-to-trunk area ratio and angle of branching that were comparable to the human abdominal aorta. Velocity profiles were measured with a laser Doppler anemometer during steady and pulsatile flow in which th...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1982-191-233
更新日期:1982-01-01 00:00:00
abstract::Different methods are commonly used to study the red blood cell aggregation phenomenon. The major interest of the ultrasonic method presently discussed is to assess the mean size of red blood cell (RBC) aggregates by measuring ultrasonic intensity backscattered by blood. Applying Rayleigh theory of sound to blood medi...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-27104
更新日期:1990-01-01 00:00:00
abstract::The electrical properties of red blood cell (RBC) suspensions are influenced by flow conditions, and prior studies indicate that electrical properties may reflect the kinetics of RBC aggregation. Changes of conductance and capacitance were monitored and had a time course resembling a "syllectogram" (i.e., temporal cha...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2009-0541
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Exposure to particulate air pollution is associated with an increased risk of cardiovascular disease. The mechanism by which exposure increases risk is poorly understood but could involve changes in the flow properties of blood. OBJECTIVE:The aim of this investigation was to assess the effect, in rats, of i...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-14027
更新日期:2014-01-01 00:00:00
abstract::The aim of this study was to investigate the effects of a surgical limb lengthening procedure on the biorheological features of some lengthened soft tissues. In this procedure external fixators were applied to goats' right radius to stretch the tissues. The right forelegs of goats were lengthened by 2, 4 cm, respectiv...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/S0006-355X(99)80016-X
更新日期:1998-07-01 00:00:00
abstract::During the recent years various investigators have developed the technique of kryophotometric measurements of oxyhemoglobin saturation with oxygen (psi) in small vessels. In this paper, on the basis of the statistical model of the organ developed by the authors, a theoretical relationship is established between the di...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1982-191-235
更新日期:1982-01-01 00:00:00
abstract::A non-biodegradable polyhedral oligomeric silsesquioxane (POSS) nanocomposite biopolymer has been developed for fabrication of medical devices and for tissue engineering human organs. The polymer in solution, containing 2 wt% of POSS, has been synthesized, characterized and investigated to determine its key rheologica...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:BACKGROUND:Blood flow in stenotic vessels strongly influences the progression of vascular diseases. Plaques in stenotic blood vessels vary in stiffness, which influences plaque behavior and deformation by pressure and flow. Concurrent changes in plaque geometry can, in turn, affect blood flow conditions. Thus, simultan...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-14016
更新日期:2015-01-01 00:00:00
abstract::The blood flow immediately adjacent to the wall of a blood vessel or an artificial surface is of great interest. This flow defines the shear stress at the wall and is known to have a great physiological importance. The use of models is a viable method to investigate this flow. However, even in models the shear stress ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The role of clustered of L-selectin receptors on the leukocyte surface is discussed in connection with the postulated velocity-dependent formation of selectin-ligand tether bonds to interpret the mechanism of leukocyte tethering to, and rolling along, the vascular endothelium. The distinct feature of this step-wise pr...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Ciliary metachronism and motility were examined optically in muco-ciliary tissue cultures from three different systems: a) frog's palate epithelium, b) frog's oesophagus, and c) human nasal polyps. In addition, lateral cilia of Mytilus edulis (water transporting cilia) were examined. It was revealed that the degree of...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-273-433
更新日期:1990-01-01 00:00:00
abstract::Rheological measurements on a high molecular weight Hyaluronic Acid (from rooster combs) were performed to obtain information on the viscoelastic and temporary network characteristics of Hyaluronic Acid solutions as a function of the concentration. From rheological experiments, the critical concentration c* and the co...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:1993-01-01 00:00:00
abstract::The goal of this study was to determine the duration of time that ligaments from a study group need to be loaded in order to adequately determine their collective viscoelastic behavior. Rat ligaments were subjected either to creep or stress relaxation for 1,000 s or stress relaxation for 10,000 s to compare estimates ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The static and dynamic viscoelastic properties were studied of longitudinal and circumferential strips excised from various large veins of dogs. The mechanical behavior in longitudinal direction could be regarded as elastic, while that in circumferential direction was highly viscoelastic. No distinct regionality was o...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20510
更新日期:1983-01-01 00:00:00
abstract::Mechanical forces can stimulate the production of extracellular matrix molecules. We tested the efficacy of ultrasound to increase proteoglycan synthesis in bovine primary chondrocytes. The ultrasound-induced temperature rise was measured and its contribution to the synthesis was investigated using bare heat stimulus....
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::In this work, we studied the effects of tensile strain on limb bud mesenchymal cells (MSC) cultured on a collagen type I tubular scaffold. A novel bioreactor was designed to culture the cells while subjecting the tubular scaffold to tensile stress and strain. Control samples included unseeded and MSC-seeded tubes cult...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2009-0553
更新日期:2009-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have the potential to differentiate into distinct mesenchymal tissue cells. They are easy to expand while maintaining their undifferentiated state, which suggests that these cells could be an attractive cell source for tissue engineering of cartilage. In vitro high density micromass cultu...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The maintenance of articular cartilage integrity requires a balance between anabolic and catabolic processes which are under the control of chondrocytes. These cells are living in an anaerobic environment and normally do not divide. They are responsible for the continuous maintenance of the cartilage extracellular mat...
journal_title:Biorheology
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00