Abstract:
:Mechanical forces can stimulate the production of extracellular matrix molecules. We tested the efficacy of ultrasound to increase proteoglycan synthesis in bovine primary chondrocytes. The ultrasound-induced temperature rise was measured and its contribution to the synthesis was investigated using bare heat stimulus. Chondrocytes from five cellular isolations were exposed in triplicate to ultrasound (1 MHz, duty cycle 20%, pulse repetition frequency 1 kHz) at average intensity of 580 mW/cm2 for 10 minutes daily for 1-5 days. Temperature evolution was recorded during the sonication and corresponding temperature history was created using a controllable water bath. This exposure profile was used in 10-minute-long heat treatments of chondrocytes. Heat shock protein 70 (Hsp70) levels after one-time treatment to ultrasound and heat was analyzed by Western blotting, and proteoglycan synthesis was evaluated by 35S-sulfate incorporation. Ultrasound treatment did not induce Hsp70, while heat treatment caused a slight heat stress response. Proteoglycan synthesis was increased approximately 2-fold after 3-4 daily ultrasound stimulations, and remained at that level until day 5 in responsive cell isolates. However, chondrocytes from one donor cell isolation out of five remained non-responsive. Heat treatment alone did not increase proteoglycan synthesis. In conclusion, our study confirms that pulsed ultrasound stimulation can induce proteoglycan synthesis in chondrocytes.
journal_name
Biorheologyjournal_title
Biorheologyauthors
Kopakkala-Tani M,Leskinen JJ,Karjalainen HM,Karjalainen T,Hynynen K,Töyräs J,Jurvelin JS,Lammi MJsubject
Has Abstractpub_date
2006-01-01 00:00:00pages
271-82issue
3,4eissn
0006-355Xissn
1878-5034journal_volume
43pub_type
杂志文章相关文献
BIORHEOLOGY文献大全abstract::While not generally viewed as physiologically significant in articular cartilage, substantial tension can develop in fibrocartilage structures and in articular cartilage injuries. This study examined how different amplitudes of cyclic tension influence chondrocyte matrix synthesis. Bovine articular chondrocytes seeded...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract:BACKGROUND:RRx-001 is an anti-cancer immunotherapeutic that increases the sensitivity of drug resistant tumors via multiple mechanisms which involve binding to hemoglobin and enhancing nitrite reductase activity of deoxyhemoglobin. OBJECTIVE:In the present study, the effect of clinically used doses of RRx-001 on eryth...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-190213
更新日期:2019-01-01 00:00:00
abstract::The assessment of vessel patency can be substantially improved by serial microvessel diameter measurements taken successively along an extensive length of the vessel. It is possible to avoid making the a priori assumptions about the existence or location of local constriction sites implicit in single diameter measurem...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1991-283-420
更新日期:1991-01-01 00:00:00
abstract::Platelet margination (enhanced platelet concentration in the near wall region of a blood vessel) has been well documented in small vessels. In artery-sized vessels margination has only been demonstrated in one study, using ghost cell suspensions and under relatively non-physiologic conditions of steady flow and 50 cm ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The membrane shear elastic modulus (mu) and the time constant for extensional shape recovery (tc) were measured for normal, control human red blood cells (RBC) and for RBC heat treated (HT) at 48 degrees C. Three separate methods for the measurement of mu were compared (two used a micropipette and one employed a flow ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1985-22106
更新日期:1985-01-01 00:00:00
abstract:BACKGROUND:Bile, which is secreted by the liver, is essential for digesting fat and maintaining homeostasis. Although the rheology of bile is very important to its flow, its extensional viscosity has not been studied. OBJECTIVE:We investigated the stretching of bile and evaluated its extensional viscosity using a fila...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-190216
更新日期:2019-01-01 00:00:00
abstract::The separation process of blood and RBC suspensions in a hematocrit range between 0.3-0.7 was investigated with a centrifuge allowed to run at low accelerations (100 xg-1000 xg). The position of the interface between the supernatant of plasma and the RBC column was continuously recorded by a new optoelectronic measuri...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1988-251-233
更新日期:1988-01-01 00:00:00
abstract::The aim of this study was to investigate the effects of a surgical limb lengthening procedure on the biorheological features of some lengthened soft tissues. In this procedure external fixators were applied to goats' right radius to stretch the tissues. The right forelegs of goats were lengthened by 2, 4 cm, respectiv...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/S0006-355X(99)80016-X
更新日期:1998-07-01 00:00:00
abstract::Arterial hemodynamic forces may play a role in the localization of early atherosclerotic lesions. We have been developing numerical techniques based on overset or "Chimera" type formulations to solve the Navier-Stokes equations in complex geometries simulating arterial bifurcations. This paper presents three-dimension...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.1016/s0006-355x(99)80020-1
更新日期:1998-11-01 00:00:00
abstract::We present data which show that fibrin gels are ordered network structures, the porosity of which is determined by the amount of thrombin being present during the activation step preceding gelation. On activation of fibrinogen, fibrinopeptides are released and simultaneously polymers are formed, obeying apparent first...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1984-211-214
更新日期:1984-01-01 00:00:00
abstract::Ciliary metachronism and motility were examined optically in tissue cultures from frog palate epithelium as a function of extracellular ATP concentration in the range of 10(-7)-10(-3) M. The main findings were: a) upon addition of ATP the metachronal wavelength increased by a factor of up to 2. b) the velocity of the ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1988-25309
更新日期:1988-01-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) have the potential to differentiate into distinct mesenchymal tissue cells. They are easy to expand while maintaining their undifferentiated state, which suggests that these cells could be an attractive cell source for tissue engineering of cartilage. In vitro high density micromass cultu...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The aggregation of red blood cells may be analyzed as an interaction of an adhesive surface energy and the elastic stored energy which results from deformation of the cell. The adhesive surface energy is the work required to separate a unit adhered area and is the resultant of adhesive forces due to the bridging molec...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1984-21406
更新日期:1984-01-01 00:00:00
abstract::In this work, we studied the effects of tensile strain on limb bud mesenchymal cells (MSC) cultured on a collagen type I tubular scaffold. A novel bioreactor was designed to culture the cells while subjecting the tubular scaffold to tensile stress and strain. Control samples included unseeded and MSC-seeded tubes cult...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-2009-0553
更新日期:2009-01-01 00:00:00
abstract::Over the past several decades, blood-soluble drag reducing polymers (DRPs) have been shown to significantly enhance hemodynamics in various animal models when added to blood at nanomolar concentrations. In the present study, the effects of the DRPs on blood circulation were tested in anesthetized rats exposed to acute...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Cervical mucus glycoproteins (mucins) were extracted by using slow stirring in 6M-guanidinium chloride supplemented with proteinase inhibitors. Subsequent purification was achieved by isopycnic density-gradient centrifugation in CsCl/guanidinium chloride. The whole mucins (Mr 10 X 10(6) - 15 X 10(6)) were degraded int...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1984-211-225
更新日期:1984-01-01 00:00:00
abstract::Mechanical forces influence articular cartilage structure by regulating chondrocyte activity. Mechanical stimulation results in activation of an alpha5beta1 integrin dependent intracellular signal cascade involving focal adhesion kinase and protein kinase C, triggering the release of interleukin-4 from the cell. In no...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Pentoxifylline (PTX) has been reported to enhance the early accumulation of neutrophils at the site of Staphylococcus aureus subcutaneous infection in mice (1) and to stimulate in vitro PMN chemotaxis, particularly under dense agarose (2). Among the biochemical events contributing to chemotaxis are actin polymerizatio...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-273-421
更新日期:1990-01-01 00:00:00
abstract::The viscosity and the order in the interior of human erythrocyte membranes were investigated by the fluorescence depolarization technique in the nanosecond region with 1,6-diphenyl-1,3,5-hexatriene (DPH). After pulsed excitation with a polarized light, the fluorescence anisotropy ratio of DPH in membranes rapidly decr...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1986-23504
更新日期:1986-01-01 00:00:00
abstract:BACKGROUND:Red blood cell (RBC) deformability may increase, or decrease, following application of shear stress ("shear conditioning"), depending upon the specific magnitude and duration of exposure. However, the time course of altered RBC deformability following shear remains unresolved. OBJECTIVE:We utilised shear co...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-17151
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Blood flow in stenotic vessels strongly influences the progression of vascular diseases. Plaques in stenotic blood vessels vary in stiffness, which influences plaque behavior and deformation by pressure and flow. Concurrent changes in plaque geometry can, in turn, affect blood flow conditions. Thus, simultan...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-14016
更新日期:2015-01-01 00:00:00
abstract::The presented paper is based on the conclusions of the preceding paper (1). On the basis of a general theoretical approach to the mechanics of heterogeneous materials published earlier / (2) , (3) , (4) / macroscopic constitutive equation of trabecular bone is deduced from the description of its microstructure. For th...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20607
更新日期:1983-01-01 00:00:00
abstract::The fractionation of micron-size particles according to physical properties of size, density and surface characteristics by centrifugation and electrophoresis is hindered when the particles behave collectively rather than individually. The formation and sedimentation of droplets containing particles is an extreme exam...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1983-20201
更新日期:1983-01-01 00:00:00
abstract::Flow induced shear stress influences vascular cellular biology and pathophysiology in numerous ways. Previous in vitro studies on interactions between flow and endothelial cells using parallel-plate flow chambers involve two-dimensional flows, whereas flows in larger vessels are commonly three-dimensional. We have con...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Flow-dependent concentration or depletion of atherogenic low density lipoproteins which has been theoretically predicted to occur at a blood/endothelium boundary may play an important role in the genesis, progression, and regression of atherosclerosis in man and intimal hyperplasia in vascular grafts implanted in the ...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Ciliary metachronism and motility were examined optically in muco-ciliary tissue cultures from three different systems: a) frog's palate epithelium, b) frog's oesophagus, and c) human nasal polyps. In addition, lateral cilia of Mytilus edulis (water transporting cilia) were examined. It was revealed that the degree of...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/bir-1990-273-433
更新日期:1990-01-01 00:00:00
abstract:BACKGROUND:In order to play different roles in vascular functions as a mechanosensor to blood flows and as a barrier to transvascular exchange, the endothelial surface glycocalyx (ESG) should have an organized structure. Due to the limitations of optical and electron microscopy, the ultra-structure of ESG has not been ...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-180204
更新日期:2019-01-01 00:00:00
abstract::In this paper we shall describe new mechanical models for the deformation of the actin filament bundles in kidney microvilli and osteocytic cell processes to see whether these cellular extensions, like the stereocilia on hair cells in the inner ear, can function as mechanotransducers when subject to physiological flow...
journal_title:Biorheology
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:Devices gauging viscoelastic properties of blood during coagulation like the thromboelastograph support fundamental research as well as point of care needs. Associated fibrinolysis data are based on endogenous species or plasminogen activator added to a homogeneous sample prior to clot formation. Digestion i...
journal_title:Biorheology
pub_type: 杂志文章
doi:10.3233/BIR-15089
更新日期:2016-07-29 00:00:00
abstract::The application of principles of biorheology, hemorheology and perihemorheology on problems of the nervous system in health and disease was suggested by Alfred L. Copley (1982, 1987). Late in 1988 Copley and Sourander considered neurobiorheology to be an appropriate term for a new branch of biorheology bridging the ga...
journal_title:Biorheology
pub_type: 历史文章,杂志文章
doi:10.3233/bir-1991-28603
更新日期:1991-01-01 00:00:00