Abstract:
:Primers were used to amplify a 561-bp region of the 16S rRNA gene of Ehrlichia phagocytophila from Ixodes scapularis ticks and small mammals collected in Rhode Island and Connecticut. DNA sequences for all 50 E. phagocytophila-positive samples collected from 1996 through 1998 in southwestern Connecticut were identical to the sequence reported for E. phagocytophila DNA from confirmed human cases. In contrast, the sequences from 92 of 123 E. phagocytophila-positive Rhode Island samples collected from 1996 through 1999 included several variants differing by 1-2 nucleotides from that in the agent infecting humans. While 11.9% of 67 E. phagocytophila-positive ticks collected during 1997 in Rhode Island harbored ehrlichiae with sequences identical to that of the human agent, 79.1% had a variant sequence not previously described. The low incidence of human ehrlichiosis in Rhode Island may in part result from interference by these variant ehrlichiae with maintenance and transmission of the true agent of human disease.
journal_name
Emerg Infect Disjournal_title
Emerging infectious diseasesauthors
Massung RF,Mauel MJ,Owens JH,Allan N,Courtney JW,Stafford KC 3rd,Mather TNdoi
10.3201/eid0805.010251subject
Has Abstractpub_date
2002-05-01 00:00:00pages
467-72issue
5eissn
1080-6040issn
1080-6059journal_volume
8pub_type
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