CrAssphage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands.

Abstract:

:CrAssphage is a recently discovered human gut-associated bacteriophage. To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. CrAssphage sequences could be grouped into 2 major genetic clusters. Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands.

journal_name

Emerg Infect Dis

authors

Park GW,Ng TFF,Freeland AL,Marconi VC,Boom JA,Staat MA,Montmayeur AM,Browne H,Narayanan J,Payne DC,Cardemil CV,Treffiletti A,Vinjé J

doi

10.3201/eid2608.200346

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

1731-1739

issue

8

eissn

1080-6040

issn

1080-6059

journal_volume

26

pub_type

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