Effect of IFN-gamma on expression of HLA in bare-lymphocyte syndrome-like cell line HAJ.

Abstract:

:We compared HLA antigen expression on new B-lymphoblastoid cell line (B-LCL) HAJ with that on B-LCLs expressing normal HLA levels as well as on B-LCLs derived from bare lymphocyte syndrome (BLS) patients and in vitro mutated B-LCLs of BLS-like phenotype. HAJ cells had no expression of HLA class II and low expression of class I antigens similarly to some of BLS B-LCLs, although HAJ cell line was derived from lymphocytes of HLA class I- and class II-normally expressing donor. HAJ cells displayed B lymphocyte markers, surface immunoglobulin and CD19. Culture of HAJ cells in the presence of interferon y resulted in HLA class I antigen upregulation, but did not restore class II expression. The cell line HAJ may prove useful for studies on factors influencing HLA class I cell surface expression.

authors

Nowak I,Pochroń B,Kozlowska A,Dubis J,Kuśnierczyk P

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

453-60

issue

6

eissn

0004-069X

issn

1661-4917

journal_volume

49

pub_type

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