GTP hydrolysis of cell division protein FtsZ: evidence that the active site is formed by the association of monomers.

abstract：:The properties of Na,K-ATPase phosphoenzymes formed either from ATP in the presence of Mg2+ and Na+ or from Pi in the absence of alkali cations were investigated by biochemical methods and spectrofluorometry employing the styryl dye RH421. We characterized the phosphoenzyme species by their reaction to N-methyl hydrox...

journal_title：Biochemistry

authors： Fedosova NU,Cornelius F,Klodos I

更新日期：1998-09-29 00:00:00

abstract：:Amyloid-beta (Abeta) peptide deposition as fibrillar senile plaques is a key element in the pathology of Alzheimer's disease. Here we present a high-resolution structure of an Abeta amyloid fibril using magnetically aligned preparations of a central Abeta domain which forms representative amyloid fibrils. Diffraction ...

journal_title：Biochemistry

authors： Serpell LC,Blake CC,Fraser PE

更新日期：2000-10-31 00:00:00

abstract：:Protein kinase C-related kinases (PRKs) are serine/threonine kinases that are members of the protein kinase C superfamily and can be activated by binding to members of the Rho family of small G proteins via a Rho binding motif known as an HR1 domain. The PRKs contain three tandem HR1 domains at their N-termini. The st...

journal_title：Biochemistry

authors： Hutchinson CL,Lowe PN,McLaughlin SH,Mott HR,Owen D

更新日期：2011-04-12 00:00:00

abstract：:The bidirectional transformation of a protein between its native water-soluble and integral transmembrane conformations is demonstrated for FraC, a hemolytic protein of the family of pore-forming toxins. In the presence of biological membranes, the water-soluble conformation of FraC undergoes a remarkable structural r...

journal_title：Biochemistry

authors： Tanaka K,Caaveiro JM,Tsumoto K

更新日期：2015-11-24 00:00:00

abstract：:NMR studies of protein denatured states provide insights into potential initiation sites for folding that may be too transient to be observed kinetically. We have characterized the structure and dynamics of the acid-denatured state of protein G by using a F30H mutant of G(B1) which is on the margin of stability. At 5 ...

journal_title：Biochemistry

authors： Sari N,Alexander P,Bryan PN,Orban J

更新日期：2000-02-08 00:00:00

abstract：:An RNA that replicates with core RNA polymerase from E. coli and the substrates ATP, CTP, ITP, and UTP, was selected from a random poly(A,U,I,C) library and named EcorpI. Another replicating RNA, EcorpG, was obtained by template-free incubation of holo RNA polymerase and the substrates ATP, CTP, GTP, and UTP. Both RNA...

journal_title：Biochemistry

authors： Wettich A,Biebricher CK

更新日期：2001-03-20 00:00:00

abstract：:The combination of UV/visible/near-IR variable-temperature magnetic circular dichroism (VTMCD) and EPR spectroscopies has been used to investigate the spin states and axial ligation of the heme group in oxidized, reduced, and CO-bound reduced forms of the Rhodospirillum rubrum CO oxidation transcriptional activator pr...

journal_title：Biochemistry

authors： Dhawan IK,Shelver D,Thorsteinsson MV,Roberts GP,Johnson MK

更新日期：1999-09-28 00:00:00

abstract：:H1 can rearrange in chicken erythrocyte polynucleosomes in 80 mM NaCl buffers. These rearrangements have been studied by sedimentation analysis. H1 redistributes between polynucleosomes as well as between polynucleosomes and monosomes. In these rearrangements H1 molecules move to free DNA sites. In contrast to H1, the...

journal_title：Biochemistry

authors： Lasters I,Muyldermans S,Wyns L,Hamers R

更新日期：1981-03-03 00:00:00

abstract：:Drug substrates stimulate ATPase activity of the P-glycoprotein (P-gp) ATP-binding cassette drug pump by an unknown mechanism. Cross-linking analysis was performed to test if drug substrates stimulate P-gp ATPase activity by altering cross-talk at the first transmission interface linking the drug-binding [intracellula...

journal_title：Biochemistry

authors： Loo TW,Clarke DM

更新日期：2016-05-24 00:00:00

abstract：:Picosecond transient absorption spectra of rhodopsin and isorhodopsin were measured at room temperature with a double-beam laser spectrophotometer after excitation at 355 nm. Photolysis studies were performed on rhodopsin solubilized in two different detergents (digitonin and 3-[(3-cholamidopropyl)dimethylammonio]-1-p...

journal_title：Biochemistry

authors： Rudzki JE,Peters KS

更新日期：1984-08-14 00:00:00

abstract：:Deuterium nuclear magnetic resonance spectra of dimyristoylphosphatidylcholines specifically labeled in positions 2', 3', 4', 6', 8', 10', 12', and 14', of the 2 chain, of an N-deuteriomethylphosphatidylcholine, and of cholesterol-3alpha-d1, have been obtained by the Fourier transform method at 5.46 and 3.52 T on two ...

journal_title：Biochemistry

authors： Oldfield E,Meadows M,Rice D,Jacobs R

更新日期：1978-07-11 00:00:00

abstract：:The flavoprotein component (SiR-FP) of the sulfite reductase from Escherichia coli is an octamer containing one FAD and one FMN per polypeptide chain. SiR-FP60, a SiR-FP fragment starting with alanine-52, was overexpressed in E. coli and purified as a monomer. The N-terminal part of the native protein contains thus al...

journal_title：Biochemistry

authors： Zeghouf M,Fontecave M,Macherel D,Covès J

更新日期：1998-04-28 00:00:00

abstract：:The two dominant glucolipids in Acholeplasma laidlawii, viz., 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol (MGlcDG) and 1,2-diacyl-3-O-[alpha-D-glucopyranosyl-(1----2)-O-alpha-D-glucopyranosyl ]- sn-glycerol (DGlcDG), have markedly different phase behavior. MGlcDG has an ability to form nonlamellar phases, wher...

journal_title：Biochemistry

authors： Eriksson PO,Rilfors L,Wieslander A,Lundberg A,Lindblom G

更新日期：1991-05-21 00:00:00

abstract：:Several furanocoumarins were tested for their ability to inhibit human P450 2A6 activity. The metabolites and conjugates formed from these furanocoumarins after incubation with reconstituted purified P450 2A6 in the absence and presence of exogenous nucleophiles were characterized by UV and LC/ESI-MS/MS analysis. The ...

journal_title：Biochemistry

authors： Koenigs LL,Trager WF

更新日期：1998-07-14 00:00:00

abstract：:AhpF, the flavoprotein reductase component of the Salmonella typhimurium alkyl hydroperoxide reductase system, catalyzes the reduction of an intersubunit disulfide bond in the peroxidatic active site of the system's other component, AhpC, a member of the peroxiredoxin family. Previous studies have shown that AhpF can ...

journal_title：Biochemistry

authors： Reynolds CM,Poole LB

更新日期：2001-04-03 00:00:00

abstract：:The stoichiometry of cytochrome b559 (one or two copies) per reaction center of photosystem II (PSII) has been the subject of considerable debate. The molar ratio of cytochrome b559 has a number of significant implications on our understanding of the functional role of cytochrome b559, the mechanism of electron donati...

journal_title：Biochemistry

authors： Buser CA,Diner BA,Brudvig GW

更新日期：1992-11-24 00:00:00

abstract：:Transferrins have been extensively studied in order to understand how they reversibly bind and release iron. Human serum transferrin (hTF) is a single polypeptide chain that folds into two lobes (N- and C-lobe); each lobe binds a single ferric ion. Iron release induces a large conformational change in each lobe. At th...

journal_title：Biochemistry

authors： James NG,Berger CL,Byrne SL,Smith VC,MacGillivray RT,Mason AB

更新日期：2007-09-18 00:00:00

abstract：:Lysyl-tRNA synthetase, a class II enzyme, edits homocysteine by converting it into homocysteine thiolactone. In a similar reaction, the enzyme converts homoserine into homoserine lactone. Other class II enzymes, aspartyl-tRNA synthetase and seryl-tRNA synthetase, do not edit any of the amino acids tested. However, all...

journal_title：Biochemistry

authors： Jakubowski H

更新日期：1997-09-16 00:00:00

abstract：:Thermal pertubation difference spectra of sperm whale myoglobin (Mb) and soybean leghemoglobin a (Lb a) in the near-ultraviolet reveal similarities in the tryptophan environment of the two proteins. Of the two tryptophans in each protein, one has its indolyl NH group fully exposed to aqueous solvent, while the other b...

journal_title：Biochemistry

authors： Nicola NA,Leach SJ

更新日期：1977-01-11 00:00:00

abstract：:We have previously shown that GTP can replace ATP as an energy source to support vinblastine transport by the multidrug transporter P-glycoprotein (Pgp) in plasma membrane vesicles isolated from the multidrug resistant cell line KB-V1 [Lelong et al. (1992) FEBS Lett. 304, 256-260]. Like [gamma-32P]ATP, [gamma-32P]GTP ...

journal_title：Biochemistry

authors： Lelong IH,Cardarelli CO,Gottesman MM,Pastan I

更新日期：1994-08-02 00:00:00

abstract：:The structure and function of iron(II)-ruthenium(II) hybrid hemoglobins alpha(Ru-CO)2 beta(Fe)2 and alpha(Fe)2 beta(Ru-CO)2, which can serve as models for the intermediate species of the oxygenation step in native human adult hemoglobin, were investigated by measuring oxygen equilibrium curves and the Fe(II)-N epsilon...

journal_title：Biochemistry

authors： Ishimori K,Tsuneshige A,Imai K,Morishima I

更新日期：1989-10-17 00:00:00

abstract：:Campylobacter jejuni contains a general N-linked glycosylation pathway in which a heptasaccharide is sequentially assembled onto a polyisoprenyl diphosphate carrier and subsequently transferred to the asparagine side chain of an acceptor protein. The enzymes in the pathway function at a membrane interface and have in ...

journal_title：Biochemistry

authors： Chen MM,Weerapana E,Ciepichal E,Stupak J,Reid CW,Swiezewska E,Imperiali B

更新日期：2007-12-18 00:00:00

abstract：:NG-Methyl-L-arginine (L-NMA) is one of the most commonly used inhibitors of the nitric oxide synthases (NOS). Results reported here demonstrate that L-NMA is an alternate substrate and a mechanism-based inhibitor of the inducible NOS purified from murine macrophages. The irreversible inhibition displays pseudo-first-o...

journal_title：Biochemistry

authors： Olken NM,Marletta MA

更新日期：1993-09-21 00:00:00

abstract：:Anticoagulation factor II (ACF II) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein in a Ca(2+)-dependent fashion with marked anticoagulant activity. The equilibrium unfolding/refolding of apo-ACF II, holo-ACF II, and Tb(3+)-reconstituted ACF II in guanidine hydrochlor...

journal_title：Biochemistry

authors： Xu X,Liu Q,Xie Y

更新日期：2002-03-19 00:00:00

abstract：:The secretion of lung surfactant in alveolar type II cells is a complex process involving the fusion of lamellar bodies with the plasma membrane. This process is somewhat different from the exocytosis of hormones and neurotransmitters. For example, it is a relatively slower process, and lamellar bodies are very large ...

journal_title：Biochemistry

authors： Abonyo BO,Gou D,Wang P,Narasaraju T,Wang Z,Liu L

更新日期：2004-03-30 00:00:00

abstract：:MAP kinase kinase (MAPKK) is a dual specificity protein kinase that phosphorylates and activates MAP kinase in vivo. In this study, four mouse monoclonal single-chain Fv (scFv) antibodies (Y1-6, Y1-7, Y3-6, and Y3-11) that can specifically bind to Xenopus MAPKK were isolated from combinatorial scFv-displaying phage li...

journal_title：Biochemistry

authors： Kosako H,Akamatsu Y,Tsurushita N,Lee KK,Gotoh Y,Nishida E

更新日期：1996-10-08 00:00:00

abstract：:Three modified hammerhead ribozyme/substrate complexes have been prepared in which individual uridine O2-carbonyls have been eliminated. The modified complexes were chemically synthesized with the substitution of a single 2-pyridone (2P) base analogue for residues U4, U7, and U16.1. Steady-state kinetic analyses indic...

journal_title：Biochemistry

authors： Bevers S,Ha SB,McLaughlin LW

更新日期：1999-06-15 00:00:00

abstract：:Quadruplex-specific molecules can serve as suitable drugs in cancer therapy. We have synthesized a pair of furan-based cyclic homooligopeptides, ligand 1 and ligand 2, to specifically target G-quadruplexes. We have shown by CD spectroscopy and UV melting that these ligands can effectively induce G-quadruplex structure...

journal_title：Biochemistry

authors： Agarwal T,Roy S,Chakraborty TK,Maiti S

更新日期：2010-09-28 00:00:00

abstract：:Retinal rod and cone cGMP phosphodiesterases (PDE6 family) function as the effector enzyme in the vertebrate visual transduction cascade. The activity of PDE6 catalytic subunits is controlled by the Pgamma-subunits. In addition to the inhibition of cGMP hydrolysis at the catalytic sites, Pgamma is known to stimulate a...

journal_title：Biochemistry

authors： Muradov KG,Granovsky AE,Schey KL,Artemyev NO

更新日期：2002-03-26 00:00:00

abstract：:The S-adenosylmethionine (SAM) riboswitch is one of the most recurrent riboswitches found in bacteria and has three known different natural aptamers. The Bacillus subtilis yitJ SAM riboswitch aptamer is organized around a four-way junction which is characterized by the presence of a pseudoknot and a K-turn motif. By r...

journal_title：Biochemistry

authors： Heppell B,Lafontaine DA

更新日期：2008-02-12 00:00:00