Iodine speciation in human serum by reversed-phase liquid chromatography-ICP-mass spectrometry.

Abstract:

:Reversed-phase liquid chromatography-inductively coupled plasma mass spectrometric hyphenation was used for iodine speciation in human serum. First investigations showed that iodine species nearly quantitatively were eluted in the void volume. The result indicated that protein-linked thyroid hormones were not interacting with the stationary phase, thus being not retained. Investigations were performed about T4-TBG (thyroxin-thyroxin-binding globulin) complex generation and its retention during chromatography. It was shown that T4-TBG was not retained on the column. Therefore, a protease treatment was introduced for serum sample preparation. The analysis of "normal" sera (after protease) gave reasonable results lying in the range published in literature: I-:11; di-iodothyrosine (DIT): 2.1; mono-iodothyrosine (MIT): 1.6; reversed tri-iodothyronine (rT3): 3.9; T3: 5.9; T4: 60; each micrograms iodine per liter. The method also proved to recognize abnormalities in a pathologic serum, having rT3 as the predominant species. In this case the method obviously was superior compared to standard immunoassay methods, as it is monitoring the iodine in the species (physiologically active iodine species), whereas immunoassay methods may sometimes detect deiodinated (inactive) compounds.

journal_name

Biol Trace Elem Res

authors

Michalke B,Schramel P,Witte H

doi

10.1385/BTER:78:1-3:81

subject

Has Abstract

pub_date

2000-01-01 00:00:00

pages

81-91

issue

1-3

eissn

0163-4984

issn

1559-0720

pii

BTER:78:1-3:81

journal_volume

78

pub_type

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