Abstract:
OBJECTIVES:To test whether the Glu298Asp polymorphism of the endothelial nitric oxide synthase (eNOS) gene is of functional relevance in humans by altering endothelium-dependent vasodilation. BACKGROUND:The Asp298 variant of the eNOS gene product has been associated with arterial hypertension, coronary artery disease and myocardial infarction. The pathogenetic mechanism has not yet been elucidated. Since endothelium-dependent vasodilation has been shown to be impaired in these disorders, we hypothesized that the Glu298Asp polymorphism of the eNOS gene influences endothelium-dependent vasodilation. METHODS:In 80 patients with normal or elevated cholesterol, endothelium-dependent and -independent vasodilation was assessed. Forearm blood flow was measured by plethysmography in response to intra-arterial (i.a.) infusion of 12 and 48 microg/min acetylcholine and 3.2 and 12.8 microg/min nitroprusside, respectively. NG-monomethyl-L-arginine (L-NMMA) in doses of 4, 8 and 16 micromol/min was infused to test basal nitric oxide (NO) production and release. Genomic DNA was extracted from blood samples to determine the Glu298Asp polymorphism of the eNOS gene at position 1917 G/T after BanII restriction. RESULTS:Baseline parameters (age, gender, blood pressure, body mass index, cholesterol level) were similar across the genotypes. Genotype frequencies did not deviate from the Hardy-Weinberg equilibrium. No differences in forearm blood flow to i.a. acetylcholine (average increase: + 554 +/- 371%), nitroprusside or L-NMMA infusion were found across the eNOS genotypes, neither for endothelium-dependent or endothelium-independent vasodilation, nor for basal NO production and release. Our sample size of n = 80 had a power of > 80% (beta = 0.20) with a P value < 0.05 (alpha = 0.05) to detect a 200% difference in forearm blood flow response to 48 microg/min acetylcholine. CONCLUSIONS:At a power of 80%, we can exclude a relevant effect on endothelium-dependent vasodilation due to the eNOS Glu298Asp polymorphism. Thus, our functional genetic study does not suggest any biological effect of the eNOS Glu298Asp genotype on the cardiovascular system via an influence on endothelium-dependent vasodilation.
journal_name
J Hypertensjournal_title
Journal of hypertensionauthors
Schneider MP,Erdmann J,Delles C,Fleck E,Regitz-Zagrosek V,Schmieder REdoi
10.1097/00004872-200018120-00010subject
Has Abstractpub_date
2000-12-01 00:00:00pages
1767-73issue
12eissn
0263-6352issn
1473-5598journal_volume
18pub_type
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3283653ff5
更新日期:2013-12-01 00:00:00
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journal_title:Journal of hypertension
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更新日期:2018-03-01 00:00:00
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journal_title:Journal of hypertension
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更新日期:2003-09-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
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更新日期:2010-06-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
abstract:AIMS:Myocardial fibrosis is a relevant component of hypertensive heart disease (HHD). Novel cardiovascular magnetic resonance (CMR) imaging techniques have shown potential in quantification of diffuse cardiac fibrosis, with T1 mapping, and estimating preclinical cardiac dysfunction, with strain analysis. Molecular biom...
journal_title:Journal of hypertension
pub_type: 杂志文章
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199202000-00010
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:1989-04-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
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更新日期:2017-02-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
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更新日期:1998-11-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:1998-12-01 00:00:00
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journal_title:Journal of hypertension
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更新日期:1997-10-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/00004872-199303000-00013
更新日期:1993-03-01 00:00:00
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journal_title:Journal of hypertension
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更新日期:2020-02-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1097/00004872-199004000-00007
更新日期:1990-04-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0000000000000493
更新日期:2015-04-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3281268548
更新日期:2007-07-01 00:00:00
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更新日期:2014-07-01 00:00:00
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更新日期:1986-04-01 00:00:00
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更新日期:1997-01-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/01.hjh.0000170377.00591.7e
更新日期:2005-06-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
doi:10.1097/00004872-199606000-00013
更新日期:1996-06-01 00:00:00
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journal_title:Journal of hypertension
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doi:10.1097/00004872-199816040-00016
更新日期:1998-04-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 临床试验,杂志文章
doi:
更新日期:1991-01-01 00:00:00
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journal_title:Journal of hypertension
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doi:10.1097/HJH.0000000000002576
更新日期:2020-12-01 00:00:00
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journal_title:Journal of hypertension
pub_type: 杂志文章
doi:10.1097/HJH.0b013e3282f09f79
更新日期:2008-02-01 00:00:00