Protein complexes and analysis of their assembly by mass spectrometry.

abstract：:Almost two decades after their identification, the components of the mitotic checkpoint are finally revealing their structural secrets. The activation of Mad2, a central piece of the checkpoint protein machinery, is linked to the rare ability of this protein to adopt two distinct topologies. Current models of checkpoi...

journal_title：Current opinion in structural biology

authors： Mapelli M,Musacchio A

更新日期：2007-12-01 00:00:00

abstract：:Structure-specific DNA nucleases play important roles in various DNA transactions such as DNA replication, repair and recombination. These enzymes recognize loops and branched DNA structures. Recent structural studies have provided detailed insights into the functions of these enzymes. Structures of Holliday junction ...

journal_title：Current opinion in structural biology

authors： Nishino T,Ishino Y,Morikawa K

更新日期：2006-02-01 00:00:00

abstract：:Intrinsically disordered regions (IDRs) are fundamental units of protein function and regulation. Despite their inability to form a unique stable tertiary structure in isolation, many IDRs adopt a defined conformation upon binding and achieve their function through their interactions with other biomolecules. However, ...

journal_title：Current opinion in structural biology

authors： Flock T,Weatheritt RJ,Latysheva NS,Babu MM

更新日期：2014-06-01 00:00:00

abstract：:Electron cryo-microscopy (cryoEM) is used to determine structures of biological molecules, including multi-protein complexes. Maps at better than 3.0Å resolution are relatively straightforward to interpret since atomic models of proteins and nucleic acids can be built directly. Still, these resolutions are often diffi...

journal_title：Current opinion in structural biology

authors： Casañal A,Shakeel S,Passmore LA

更新日期：2019-10-01 00:00:00

abstract：:In the past few years, structural biology has begun to reveal details of some of the receptors and associated interactions responsible for protein-mediated recognition events at the cell surface. Recent data span the fields of cytokine-receptor interactions, cell adhesion molecules and viral infection. ...

journal_title：Current opinion in structural biology

authors： Stuart DI,Jones EY

更新日期：1995-12-01 00:00:00

abstract：:Phage display is the longest-standing platform among molecular display technologies. Recent developments have extended its utility to proteins that were previously recalcitrant to phage display. The technique has played a dominant role in forming the field of synthetic binding protein engineering, where novel interfac...

journal_title：Current opinion in structural biology

authors： Sidhu SS,Koide S

更新日期：2007-08-01 00:00:00

abstract：:Prediction and modeling of RNA structures has become an indispensable tool of biological research disciplines. Currently, reliable predictions require massive input of experimental data. Structure-forming elements are conventional base pairs, as well as a rapidly increasing repertoire of novel structural motifs. New d...

journal_title：Current opinion in structural biology

authors： Schuster P,Stadler PF,Renner A

更新日期：1997-04-01 00:00:00

abstract：:As a result of the recent enormous technological progress, experimental structure determination has become an integral part of the development of drugs against disease-related target proteins. The post-translational modification of proteins is an important regulatory process in living organisms; one such example is ly...

journal_title：Current opinion in structural biology

authors： Mittl PR,Grütter MG

更新日期：2006-12-01 00:00:00

abstract：:The exponential growth in the amount of genomic data published in recent years has led to increased efforts in analysing genomes for the presence of repeated sequences, which has in turn fostered the development of novel repeat recognition methods. This has resulted in a deepened understanding of the importance and ab...

journal_title：Current opinion in structural biology

authors： Heringa J

更新日期：1998-06-01 00:00:00

abstract：:Among the crystal structures of lectins determined recently, three--snowdrop lectin, jacalin and amaranthin--represent new lectin families. Their polypeptide folds share remarkably similar features and consist exclusively of beta structure. Autonomously folded beta-sheet subdomains, inter-related by a pseudothreefold ...

journal_title：Current opinion in structural biology

authors： Wright CS

更新日期：1997-10-01 00:00:00

abstract：:Recent developments in implicit solvent models may be compared in terms of accuracy and computational efficiency. Based on improvements in the accuracy of generalized Born methods and the speed of Poisson-Boltzmann solvers, it appears that the two techniques are converging to a point at which both will be suitable for...

journal_title：Current opinion in structural biology

authors： Baker NA

更新日期：2005-04-01 00:00:00

abstract：:There has been significant recent progress in the computational design of protein interactions including the creation of novel heterodimers, homodimers, nanohedra, fibril caps and a protein crystal. Essential to these successes has been the use of innovative strategies for finding binding modes that are achievable, th...

journal_title：Current opinion in structural biology

authors： Der BS,Kuhlman B

更新日期：2013-08-01 00:00:00

abstract：:Recently, atomic models of the mammalian, fungal and the bacterial fatty acid synthases type I (FAS I) were reported. Now, a wealth of functional data, collected during the last decades, can be embedded into structural frames. But there is more, which remains to be done! Our current considerations are implicitly very ...

journal_title：Current opinion in structural biology

authors： Grininger M

更新日期：2014-04-01 00:00:00

abstract：:Demand for novel GPCR modulators is increasing as the association between the GPCR signaling pathway and numerous diseases such as cancers, psychological and metabolic disorders continues to be established. In silico structure-based drug design (SBDD) offers an outlet where researchers could exploit the accumulating s...

journal_title：Current opinion in structural biology

authors： Lee Y,Lazim R,Macalino SJY,Choi S

更新日期：2019-04-01 00:00:00

abstract：:Ribonuclease P (RNase P) is one of only two known universal ribozymes and was one of the first ribozymes to be discovered. It is involved in RNA processing, in particular the 5' maturation of tRNA. Unlike most other natural ribozymes, it recognizes and cleaves its substrate in trans. RNase P is a ribonucleoprotein com...

journal_title：Current opinion in structural biology

authors： Torres-Larios A,Swinger KK,Pan T,Mondragón A

更新日期：2006-06-01 00:00:00

abstract：:Dynamin is an important component of membrane recycling at the plasma membrane and, potentially, within the cell. The role of dynamin in clathrin-mediated endocytosis has been based on numerous endocytosis assays, as well as on the discovery and gross characterization of the assembled spiral structure of dynamin. Rece...

journal_title：Current opinion in structural biology

authors： Hinshaw JE

更新日期：1999-04-01 00:00:00

abstract：:Achieving the goals of structural genomics initiatives depends on the outcomes of two groups of factors: the number and distribution of experimentally determined protein structures, and our ability to assign novel proteins to known structures (fold recognition) and use them to build models (modeling). The quality of t...

journal_title：Current opinion in structural biology

authors： Friedberg I,Jaroszewski L,Ye Y,Godzik A

更新日期：2004-06-01 00:00:00

abstract：:The major component of bacterial cell wall is peptidoglycan (PG), a complex polymer formed by long glycan chains cross-linked by peptide stems. PG is in constant equilibrium requiring well-orchestrated coordination between synthesis and degradation. The resulting cell-wall fragments can be recycled, act as messengers ...

journal_title：Current opinion in structural biology

authors： Alcorlo M,Martínez-Caballero S,Molina R,Hermoso JA

更新日期：2017-06-01 00:00:00

abstract：:Ising-like models have been remarkably successful in reproducing all the experimental data available on the equilibrium and kinetics of secondary structure formation in short peptides. Over the past two years, very similar models have been used to predict the folding of complete proteins, with encouraging results. Alt...

journal_title：Current opinion in structural biology

authors： Muñoz V

更新日期：2001-04-01 00:00:00

abstract：:Assembly line polyketide synthases (PKSs) are remarkable biosynthetic machines with considerable potential for structure-based engineering. Several types of protein-protein interactions, both within and between PKS modules, play important roles in the catalytic cycle of a multimodular PKS. Additionally, vectorial bios...

journal_title：Current opinion in structural biology

authors： Robbins T,Liu YC,Cane DE,Khosla C

更新日期：2016-12-01 00:00:00

abstract：:The application of biocatalysis for the synthesis of glycans and glycoconjugates is a well-established and successful strategy, both for small and large scale synthesis. Compared to chemical synthesis, is has the advantage of high selectivity, but biocatalysis had been largely limited to natural glycans both in terms ...

journal_title：Current opinion in structural biology

authors： Pallister E,Gray CJ,Flitsch SL

更新日期：2020-12-01 00:00:00

abstract：:Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental t...

journal_title：Current opinion in structural biology

authors： Vakser IA,Deeds EJ

更新日期：2019-04-01 00:00:00

abstract：:Understanding the structural rules that govern specific, high-affinity binding characteristic of aptamer-protein interactions is important in view of the increasing use of aptamers across many applications. From the modest number of 16 aptamer-protein structures currently available, trends are emerging. The flexible p...

journal_title：Current opinion in structural biology

authors： Gelinas AD,Davies DR,Janjic N

更新日期：2016-02-01 00:00:00

abstract：:Complementary developments in comparative genomics, protein structure determination and in-depth comparison of protein sequences and structures have provided a better understanding of the prevailing trends in the emergence and diversification of protein domains. The investigation of deep relationships among different ...

journal_title：Current opinion in structural biology

authors： Aravind L,Mazumder R,Vasudevan S,Koonin EV

更新日期：2002-06-01 00:00:00

abstract：:Force spectroscopy has developed into an indispensable tool for studying folding and binding of proteins on a single molecule level in real time. Design of the pulling geometry allows tuning the reaction coordinate in a very precise manner. Many recent experiments have taken advantage of this possibility and have prov...

journal_title：Current opinion in structural biology

authors： Zoldák G,Rief M

更新日期：2013-02-01 00:00:00

abstract：:C-type lectins are the largest and most diverse family of mammalian carbohydrate-binding proteins. They share a common protein fold, which provides the unifying basis for calcium-mediated carbohydrate recognition. Their involvement in a multitude of biological functions is remarkable. Here, we review the variety of ta...

journal_title：Current opinion in structural biology

authors： Keller BG,Rademacher C

更新日期：2020-06-01 00:00:00

abstract：:Gamma-tubulin is as ubiquitous in eukaryotes as alpha- and beta-tubulin. Rather than forming part of the microtubule wall, however, gamma-tubulin is involved in microtubule nucleation. Although gamma-tubulin concentrates at microtubule-organizing centers, it also exists in a cytoplasmic complex whose size and complexi...

journal_title：Current opinion in structural biology

authors： Wiese C,Zheng Y

更新日期：1999-04-01 00:00:00

abstract：:Fundamental biological processes are driven by diverse molecular machineries. In recent years, single-molecule fluorescence spectroscopy has matured as a unique tool in biology to study how structural dynamics of molecular complexes drive various biochemical reactions. In this review, we highlight underlying developme...

journal_title：Current opinion in structural biology

authors： Choi J,Grosely R,Puglisi EV,Puglisi JD

更新日期：2019-10-01 00:00:00

abstract：:Magic-angle spinning solid-state NMR experiments are well suited to investigating the structures and mechanisms of important proteins that are inaccessible to X-ray crystallography and solution NMR spectroscopy, including membrane proteins and disease-related protein aggregates. Good progress has been made in the deve...

journal_title：Current opinion in structural biology

authors： Thompson LK

更新日期：2002-10-01 00:00:00

abstract：:Intermediate filaments are built from one to several members of a multigene family encoding fibrous proteins that share a highly conserved hierarchic assembly plan for the formation of multistranded filaments from distinctly structured extended coiled coils. Despite the rather low primary sequence identity, intermedia...

journal_title：Current opinion in structural biology

authors： Herrmann H,Aebi U

更新日期：1998-04-01 00:00:00