Successful transplantation of bioengineered tissue replacements in patients with ocular surface disease.


PURPOSE:To bioengineer a corneal surface replacement using ex vivo expanded, cultured corneal epithelial stem cells seeded on a matrix derived from amniotic membrane and use this bioengineered graft to manage difficult ocular surface disease. METHODS:Fourteen patients with ocular surface disease unresponsive to standard medical and surgical treatments, including seven patients with presumed limbal stem cell deficiency were chosen for transplantation of a bioengineered composite corneal surface in eye each. Presumed corneal stem cells were harvested from either the patient's or related donor's limbus, expanded ex vivo, and cultivated on a carrier of modified human amniotic membrane. The resulting composite cultured tissue was transplanted to the ocular surface of the diseased eye, from which the abnormal tissue had been surgically removed. Ten patients received autologous grafts, and four received allogeneic grafts. RESULTS:A successful outcome, defined as restoration or improvement of vision, along with maintenance of corneal re-epithelialization and absence or recurrence of surface disease was obtained in 6 of the 10 patients with autologous procedures and in all 4 allogeneic transplants. Follow-up ranged 6-19 months with a mean of 13 months. CONCLUSIONS:This novel technique documents that presumed corneal epithelial stem cells can be harvested safely from the limbus, expanded successfully in vitro, and grown on denuded amniotic membrane. The resultant composite cultured tissue can be transplanted and appears to successfully manage eyes with difficult ocular surface disease, including those with stem cell deficiency. This technique minimizes the threat of damage or depletion to the contralateral or donor limbus.






Schwab IR,Reyes M,Isseroff RR




Has Abstract


2000-07-01 00:00:00














  • Endothelial keratoplasty without Descemet stripping after failed penetrating keratoplasty.

    abstract:PURPOSE:To evaluate outcomes of endothelial keratoplasty (EK) without Descemet membrane stripping for treatment of failed penetrating keratoplasty (PKP). METHODS:Retrospective cohort study of all eyes that underwent EK without Descemet membrane stripping for failed PKP at one institution between May 2008 and June 2010...


    pub_type: 杂志文章


    authors: Heitor de Paula F,Kamyar R,Shtein RM,Sugar A,Mian SI

    更新日期:2012-06-01 00:00:00

  • Efficacy of standardized and quality-controlled cord blood serum eye drop therapy in the healing of severe corneal epithelial damage in dry eye.

    abstract:PURPOSE:We standardized quality-controlled cord blood serum (CBS)-based eye drops and evaluated the efficacy of 1-month CBS treatment in the healing of diseased corneal epithelium in severe dry eye (DE) patients. METHODS:Seventeen graft-versus-host disease (GVHD) and 13 Sjogren syndrome patients with severe persistent...


    pub_type: 临床试验,杂志文章


    authors: Versura P,Profazio V,Buzzi M,Stancari A,Arpinati M,Malavolta N,Campos EC

    更新日期:2013-04-01 00:00:00

  • Sutured clear corneal incision: wound apposition and permeability to bacterial-sized particles.

    abstract:PURPOSE:To determine the effects of single radial or horizontal suture placement in 2-step clear corneal incision (CCI) wound apposition and permeability to particles of India ink. METHODS:Five fresh human globes were included. Two 25-gauge needles connected to a saline solution bag and to a digital manometer were ins...


    pub_type: 杂志文章


    authors: May WN,Castro-Combs J,Kashiwabuchi RT,Tattiyakul W,Qureshi-Said S,Hirai F,Behrens A

    更新日期:2013-03-01 00:00:00

  • Tolerance of Micronized Mineral Pigments for Intrastromal Keratopigmentation: A Histopathology and Immunopathology Experimental Study.

    abstract:PURPOSE:To study the tolerance and biocompatibility of mineral pigments for corneal pigmentation histopathologically and immunologically in an experimental animal model. METHODS:Manual intralamellar keratopigmentation was performed in 28 New Zealand white rabbits using micronized mineral pigments. Histopathological ex...


    pub_type: 杂志文章


    authors: Amesty MA,Rodriguez AE,Hernández E,De Miguel MP,Alio JL

    更新日期:2016-09-01 00:00:00

  • Effect of a Mediterranean dietary pattern and vitamin D levels on Dry Eye syndrome.

    abstract:PURPOSE:The aim of this study was to evaluate the association between a Mediterranean dietary pattern (MeDi) and Vitamin D levels on dry eye syndrome (DES). METHODS:This was a cross-sectional study. Male patients seen in the Miami Veterans Affairs eye clinic with normal eyelid, corneal, and conjunctival anatomy were r...


    pub_type: 杂志文章


    authors: Galor A,Gardener H,Pouyeh B,Feuer W,Florez H

    更新日期:2014-05-01 00:00:00

  • Outcomes of descemet stripping automated endothelial keratoplasty in patients with an anterior chamber versus posterior chamber intraocular lens.

    abstract:PURPOSE:The aim of this study was to assess the implications of a retained anterior chamber intraocular lens (ACIOL) during Descemet stripping automated endothelial keratoplasty (DSAEK) and to compare the outcomes in this group with those in patients who underwent DSAEK and had a posterior chamber intraocular lens (PCI...


    pub_type: 杂志文章


    authors: Shpitzer SA,Rosenblatt A,Bahar I

    更新日期:2014-07-01 00:00:00

  • Laser in situ keratomileusis flap stability during simulated aircraft ejection in a rabbit model.

    abstract:PURPOSE:To determine the stability of the laser in situ keratomileusis (LASIK) flap in a rabbit model when subjected to vertical acceleration at nine times the force of gravity (+9 Gz) in an aircraft cockpit ejection simulator. METHODS:Thirty-six eyes from 25 New Zealand white rabbits underwent LASIK flap creation wit...


    pub_type: 杂志文章


    authors: Goodman RL,Johnson DA,Dillon H,Edelhauser HF,Waller SG

    更新日期:2003-03-01 00:00:00

  • Descemet stripping automated endothelial keratoplasty for spontaneous descemet membrane detachment in a patient with osteogenesis imperfecta.

    abstract:PURPOSE:To report the first use of Descemet stripping automated endothelial keratoplasty (DSAEK) for spontaneous Descemet membrane detachment in a patient with osteogenesis imperfecta (OI), keratoconus, and acute bullous keratopathy. METHODS:A case report of a 25-year-old man with OI and symptomatic unilateral bullous...


    pub_type: 杂志文章


    authors: Gorovoy MS,Gorovoy IR,Ullman S,Gorovoy JB

    更新日期:2012-07-01 00:00:00

  • Fusarium keratitis in Mexico.

    abstract:PURPOSE:To describe the clinical characteristics, diagnosis, treatment, and visual outcome of 61 cases of Fusarium keratitis in Mexico. METHODS:Retrospective, comparative study of 61 patients with clinically and culture-confirmed Fusarium keratitis, who were attended at an eye hospital in Mexico City from 1981 to 2001...


    pub_type: 杂志文章


    authors: Pérez-Balbuena AL,Vanzzini-Rosano V,Valadéz-Virgen Jde J,Campos-Möller X

    更新日期:2009-07-01 00:00:00

  • Elevated immunoglobulin to tissue KLK11 in patients with Sjögren syndrome.

    abstract:PURPOSE:We have previously reported that mouse kallikrein (KLK) 22 in the lacrimal and salivary glands is an autoantigen that can induce primary Sjogren syndrome (SS) in rats. In this study, we determine whether the production of antibodies against tissue KLK is specific for SS and whether the antibody can be used as a...


    pub_type: 杂志文章


    authors: El Annan J,Jiang G,Wang D,Zhou J,Foulks GN,Shao H

    更新日期:2013-05-01 00:00:00

  • Efficacy of plasma rich in growth factors for the treatment of dry eye.

    abstract:PURPOSE:To evaluate the efficacy of plasma rich in growth factors (PRGF) for the treatment of moderate/severe dry eye. METHODS:PRGF treatment was administered to 16 patients who had moderate/severe dry eye diagnosed and who had not responded previously to other standard treatments. We quantified several growth factors...


    pub_type: 杂志文章


    authors: López-Plandolit S,Morales MC,Freire V,Grau AE,Durán JA

    更新日期:2011-12-01 00:00:00

  • Corneal Transplantation in New Zealand 2000 to 2009.

    abstract:PURPOSE:To quantify all minor/major adverse events in a large cohort of consecutive corneal transplants, particularly in respect to penetrating keratoplasty (PKP). METHODS:All corneal transplants in the Auckland region over a 10-year period (2000-2009) were identified through the New Zealand National Eye Bank. Relevan...


    pub_type: 杂志文章


    authors: Crawford AZ,Krishnan T,Ormonde SE,Patel DV,McGhee CN

    更新日期:2018-03-01 00:00:00

  • Reflective meniscometry: a new field of dry eye assessment.

    abstract:PURPOSE:To report a newly developed noninvasive method to measure the radius of tear meniscus curvature (reflective meniscometry) and its application to normal eyes, dry eyes, and other ocular surface conditions. METHODS:Two types of meniscometer--photographic and video--were devised. Both use tear meniscus as a conca...


    pub_type: 杂志文章


    authors: Yokoi N,Bron AJ,Tiffany JM,Kinoshita S

    更新日期:2000-05-01 00:00:00

  • Transplantation of preserved human amniotic membrane for surface reconstruction in severely damaged rabbit corneas.

    abstract::After n-heptanol removal of the total corneal epithelium and a limbal lamellar keratectomy, 23 rabbit eyes developed features of limbal stem cell deficiency including conjunctival epithelial ingrowth, vascularization and chronic inflammation. One month later, 10 control eyes received a total keratectomy, and 13 experi...


    pub_type: 杂志文章


    authors: Kim JC,Tseng SC

    更新日期:1995-09-01 00:00:00

  • Recovery of the Corneal Stroma Associated With Rapid Reepithelialization Induced by the Fibronectin-Derived Peptide PHSRN in 2 Cases of Corneal Perforation Due to a Persistent Epithelial Defect.

    abstract:PURPOSE:To report 2 cases of corneal perforation associated with a persistent epithelial defect (PED), which were treated with eye drops containing the fibronectin-derived peptide PHSRN (Pro-His-Ser-Arg-Asn). METHODS:A 67-year-old man and a 58-year-old man presented with corneal perforation associated with a PED cause...


    pub_type: 杂志文章


    authors: Morita Y,Morishige N,Yamada N,Ohta M,Sonoda KH,Nishida T

    更新日期:2015-11-01 00:00:00

  • Herpes simplex keratitis after intravitreal triamcinolone acetonide.

    abstract:PURPOSE:To report a case of recurrent herpes simplex virus (HSV) epithelial keratitis after intravitreal triamcinolone acetonide injection for the treatment of diabetic macular edema. METHODS:Case report. RESULTS:We describe a case of a 59-year-old woman with a history of ocular HSV disease and severe proliferative d...


    pub_type: 杂志文章


    authors: Shtein RM,Stahl RM,Saxe SJ,Mian SI

    更新日期:2007-06-01 00:00:00

  • Corneal involvement in congenital aniridia.

    abstract:PURPOSE:The purpose of this research is 2-fold. First of all, the level of keratopathy development in patients with congenital aniridia is studied. In addition, a correlation between the effects of ocular surgery on the severity of keratopathy is made. METHODS:A thorough search for the total number of patients with an...


    pub_type: 杂志文章


    authors: Edén U,Riise R,Tornqvist K

    更新日期:2010-10-01 00:00:00

  • A Novel Epidemiological Approach to Geographically Mapping Population Dry Eye Disease in the United States Through Google Trends.

    abstract:PURPOSE:Our study fills the spatiotemporal gaps in dry eye disease (DED) epidemiology by using Google Trends as a novel epidemiological tool for geographically mapping DED in relation to environmental risk factors. METHODS:We used Google Trends to extract DED-related queries estimating users' intent from 2004 to 2019 ...


    pub_type: 杂志文章


    authors: Azzam DB,Nag N,Tran J,Chen L,Visnagra K,Marshall K,Wade M

    更新日期:2020-11-06 00:00:00

  • Clinicohistopathological findings of gelatinous droplike corneal dystrophy among Asians.

    abstract::We describe the findings in nine Asian-Indian patients with gelatinous droplike corneal dystrophy (GDCD). Most patients showed initial signs of bilateral decreased vision and photophobia at an early age. The siblings were affected in four cases, and parental consanguinity was recorded in seven cases. We classified the...


    pub_type: 杂志文章


    authors: Li S,Edward DP,Ratnakar KS,Reddy M,Tso MO

    更新日期:1996-07-01 00:00:00

  • Altered conjunctival response after contact lens-related corneal inflammation.

    abstract:PURPOSE:Corneal inflammation in overnight contact lens (CL) wear is not fully understood. We designed a masked study to examine the impact of overnight CL wear on clinical variables in subjects who had previously experienced corneal inflammatory disease. METHODS:Forty-four subjects were entered into the study: Nine su...


    pub_type: 杂志文章


    authors: Stapleton F,Ramachandran L,Sweeney DF,Rao G,Holden BA

    更新日期:2003-07-01 00:00:00

  • Pachymetry-guided intrastromal air injection ("pachy-bubble") for deep anterior lamellar keratoplasty.

    abstract::To evaluate an innovative technique for intrastromal air injection to achieve deep anterior lamellar keratoplasty (DALK) with bare Descemet membrane (DM). Thirty-four eyes with anterior corneal pathology, including 27 with keratoconus, underwent DALK. After 400 μm trephination with a suction trephine, ultrasound pachy...


    pub_type: 杂志文章


    authors: Ghanem RC,Ghanem MA

    更新日期:2012-09-01 00:00:00

  • The BioMask for treatment of corneal surface irregularities with excimer laser phototherapeutic keratectomy.

    abstract:PURPOSE:To assess the characteristics of BioMask as a potential masking agent for use with the excimer laser. METHOD:We addressed ablation rate, smoothness, ease of use, dioptric shift, treatment of standardized irregular topography, and ability of BioMask to induce dioptric change in vivo. RESULTS:BioMask ablates at...


    pub_type: 杂志文章


    authors: Stevens SX,Bowyer BL,Sanchez-Thorin JC,Rocha G,Young DA,Rowsey JJ

    更新日期:1999-03-01 00:00:00

  • Femtosecond laser-assisted inverted mushroom keratoplasty.

    abstract:PURPOSE:To evaluate best-corrected visual acuity (BCVA), refractive outcome, corneal topography, optical coherence tomography, and endothelial cell density 12 months after femtosecond laser-assisted inverted mushroom keratoplasty. METHODS:We performed a prospective study of a surgical case series of 5 patients undergo...


    pub_type: 杂志文章


    authors: Cheng YY,Tahzib NG,van Rij G,van Cleynenbreugel H,Pels E,Hendrikse F,Nuijts R

    更新日期:2008-07-01 00:00:00

  • Modified deep anterior lamellar keratoplasty in the management of small and large epithelialized descemetoceles.

    abstract:PURPOSE:Descemetocele is a severe complication of corneal ulceration associated with a high risk of perforation. We describe a modified air-assisted deep anterior lamellar keratoplasty (DALK) technique for the surgical management of small and large epithelialized descemetoceles. METHODS:Two representative cases are pr...


    pub_type: 杂志文章


    authors: Gabison EE,Doan S,Catanese M,Chastang P,Ben M'hamed M,Cochereau I

    更新日期:2011-10-01 00:00:00

  • MT1-MMP cleavage of the antiangiogenic proteoglycan decorin: role in corneal angiogenesis.

    abstract:PURPOSE:Decorin is a small chondroitin sulfate proteoglycan that inhibits vascular endothelial cell migration and tube formation. Membrane type 1-matrix metalloproteinase (MT1-MMP) has been shown to be an important angiogenic enzyme in the cornea. We evaluated the specific role of MT1-MMP in decorin cleavage in the cor...


    pub_type: 杂志文章,收录出版


    authors: Mimura T,Chang JH,Kim TI,Onguchi T,Kojima T,Sakimoto T,Azar DT

    更新日期:2011-10-01 00:00:00

  • Topical cyclosporine in the management of shield ulcers.

    abstract:PURPOSE:To report our experience with 0.05%-2% topical cyclosporine in the treatment of 4 cases of severe, corticosteroid-resistant, vernal keratoconjunctivitis with shield ulcers. METHODS:Four patients with shield ulcers not responding to medical treatment with topical steroids, antihistamines, and mast-cell stabiliz...


    pub_type: 杂志文章


    authors: Cetinkaya A,Akova YA,Dursun D,Pelit A

    更新日期:2004-03-01 00:00:00

  • Donor-related risk factors and preoperative recipient-related risk factors for graft failure.

    abstract:PURPOSE:The aim of this study was to evaluate the outcome of penetrating keratoplasties, at the University Eye Hospital, Ludwig-Maximilians-University, Munich, Germany, using organ-cultured donor corneas and to identify preoperative risk factors, which may influence the event of graft failure. METHODS:In this study, 3...


    pub_type: 杂志文章


    authors: Yu AL,Kaiser M,Schaumberger M,Messmer E,Kook D,Welge-Lussen U

    更新日期:2014-11-01 00:00:00

  • Descemet Membrane Endothelial Keratoplasty After Failed Penetrating Keratoplasty.

    abstract:PURPOSE:To demonstrate the feasibility of Descemet membrane endothelial keratoplasty (DMEK) performed after previous penetrating keratoplasty (PK) failure and to describe primary outcomes. METHODS:Twenty-eight eyes of 28 patients who underwent DMEK after primary PK failure between January 2013 and November 2017 were i...


    pub_type: 杂志文章


    authors: Pierné K,Panthier C,Courtin R,Mazharian A,Souedan V,Gatinel D,Saad A

    更新日期:2019-03-01 00:00:00

  • Factors involved in the occurrence of bleeding complications after enucleation for cornea donation.

    abstract:PURPOSE:To identify risk factors for the occurrence of bleeding complications after enucleation for cornea donation, in order to develop preventive activities to reduce the occurrence of bleeding complications and especially the development of ocular hematomas. METHODS:From all Dutch cornea donors deceased in the year...


    pub_type: 杂志文章


    authors: van Wijk MJ,Nijenhuis MV,Dorrepaal CA,Bokhorst AG

    更新日期:2009-10-01 00:00:00

  • Corneal trephination with the femtosecond laser.

    abstract:PURPOSE:To evaluate the feasibility and cut quality of corneal trephination in human donor corneal tissue with the femtosecond laser. METHODS:Twelve human corneoscleral discs were inserted in an artificial anterior chamber. After corneal thickness measurement and tonometry, the cornea was mounted on a femtosecond lase...


    pub_type: 杂志文章


    authors: Meltendorf C,Schroeter J,Bug R,Kohnen T,Deller T

    更新日期:2006-10-01 00:00:00