Abstract:
PURPOSE:The efficacy of gene therapy using nonviral vector based on polyplex micelle has been studied against corneal neovascularization in mice. METHODS:A block copolymer, poly(ethylene glycol) (PEG)-block-polycation carrying ethylenediamine units in the side chain (PEG-b-P[Asp(DET)]), was prepared. PEG-b-P[Asp(DET)] formed a polyplex micelle through the polyion complex formation with plasmid DNA. To evaluate in vivo gene transfer efficiency, PEG-b-P[Asp(DET)] micelle was injected into the subconjunctival space of mice, and the expression of the reporter gene was assessed. Furthermore, mouse corneal neovascularization models were treated with the PEG-b-P[Asp(DET)] polyplex micelle containing expression plasmid vector of soluble vascular endothelial growth factor receptor 1 (sflt-1). RESULTS:Subconjunctival injection of the PEG-b-P[Asp(DET)] polyplex micelle containing a reporter gene showed prolonged gene expression with low cytotoxicity. Also, gene transfer into subconjunctival space by the polyplex micelle containing sflt-1 plasmid showed significant inhibition of corneal neovascularization in mice. CONCLUSIONS:Nonviral gene therapy using PEG-b-P[Asp(DET)] polyplex micelle may have potential for safe and effective therapeutic treatment of corneal neovascularization.
journal_name
Corneajournal_title
Corneaauthors
Iriyama A,Usui T,Yanagi Y,Amano S,Oba M,Miyata K,Nishiyama N,Kataoka Kdoi
10.1097/ICO.0b013e318206c893subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1423-7issue
12eissn
0277-3740issn
1536-4798journal_volume
30pub_type
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