Abstract:
:Choline-O-acetyltransferase (ChAT) is the enzyme which catalyses the biosynthesis of the neurotransmitter acetylcholine in cholinergic neurons. Here we show that in mouse cholinergic NS-20Y neuroblastoma cells cultured in the presence of either okadaic acid (serine/threonine phosphatases 1 and 2A inhibitor) or KN-62 (CaM kinase inhibitor) ChAT activity and mRNA either increased or decreased as a function of the drug concentration, respectively. After 24 h exposure, okadaic acid exerted a dramatic effect on cell morphology; cells became round and had no more neurites. On the contrary, KN-62 induced a slight morphological differentiation of the cells. The present results suggest that phosphatases 1 and 2A and CaM kinase could mediate regulation of ChAT gene expression.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Pahud G,Bontron S,Eder-Colli Ldoi
10.1016/s0197-0186(00)00064-4subject
Has Abstractpub_date
2001-01-01 00:00:00pages
75-82issue
1eissn
0197-0186issn
1872-9754pii
S0197-0186(00)00064-4journal_volume
38pub_type
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