当前位置： SCI文献检索 > JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY期刊下所有文献 > Defective DNA repair genes in a primary culture of human renal cell carcinoma.

Defective DNA repair genes in a primary culture of human renal cell carcinoma.

Abstract：

PURPOSE:Genomic stability is maintained by error-free DNA replication, repair, and recombination. To determine if repair genes contribute to genomic instability, we used a newly established cell line RCC-AJR (from clear-cell renal cell carcinoma) to examine hMSH2 (a mismatch-repair gene) and the gene encoding DNA beta polymerase (polbeta; a known contributor to base-excision repair). METHODS:Coding sequences of hMSH2 and polbeta were amplified by the polymerase chain reaction (PCR) using RNA from RCC-AJR cells and matched normal kidney (NK) cells from the same patient. Nucleotide sequences of the PCR products were determined by the dideoxy-DNA method and direct sequencing. Expressions of repair genes were assayed by Western blotting. Microsatellite stability in RCC-AJR cells was assayed by alteration in (CA)n repeats. RESULTS:In the RCC-AJR cells, we detected (a) a deletion of 1476 bp encoding 492 amino acids of hMSH2 cDNA, (b) an 87-bp deletion in the polbeta coding sequence, (c) truncated forms of hMSH2 and polbeta proteins, and (d) microsatellite instability. CONCLUSIONS:This study provides evidence of alterations in hMSH2 and polbeta in the homogeneous cell population of an RCC-AJR tumor culture. The data indicate that repair genes may help preserve genomic stability in this cell line. We believe that this new primary RCC-AJR cell line will prove a useful model for investigating the cascade of genetic events in renal cells that leads to renal carcinogenesis.

journal_name

J Cancer Res Clin Oncol

journal_title

Journal of cancer research and clinical oncology

authors

Chen HC,Bhattacharyya N,Wang L,Recupero AJ,Klein EA,Harter ML,Banerjee S

doi

10.1007/s004320050031

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

185-90

issue

4

eissn

0171-5216

issn

1432-1335

journal_volume

126

pub_type

杂志文章

相关文献

JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY文献大全
  • Adjuvant chemotherapy in elderly patients with primary breast cancer: are women ≥65 undertreated?

    abstract：PURPOSE:To establish whether women over 65 years of age with newly diagnosed with breast cancer (BC) receive adjuvant chemotherapy less frequently than younger postmenopausal women and whether comorbidity influences this potential undertreatment. MATERIALS AND METHODS:In a single-site, retrospective, comparative study...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-016-2194-4

    authors： Wallwiener CW,Hartkopf AD,Grabe E,Wallwiener M,Taran FA,Fehm T,Brucker SY,Krämer B

    更新日期：2016-08-01 00:00:00

  • Preclinical evaluation of 9-chloro-2-methylellipticinium acetate alone and in combination with conventional anticancer drugs for the treatment of human brain tumor xenografts.

    abstract：:Some ellipticine derivative salts, including 9-chloro-2-methylellipticinium (CME), have been found to have a marked selectivity against all eight brain tumor cell lines of the U.S. National Cancer Institute's disease-oriented in vitro screen. We initiated in vivo antitumor studies to explore the feasibility for furthe...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320050128

    authors： Arguello F,Alexander MA,Greene JF Jr,Stinson SF,Jorden JL,Smith EM,Kalavar NT,Alvord WG,Klabansky RL,Sausville EA

    更新日期：1998-01-01 00:00:00

  • Reduced oxygenation in a rat mammary carcinoma after chemo- or radiation therapy and reoxygenation with perflubron emulsion/carbogen breathing.

    abstract：:Rat 13672 mammary carcinoma tumors were grown subcutaneously in the hind legs of female Fischer 344 rats to a volume of about 1 cm3. Tumor oxygenation was measured using an Eppendorf PO2 histograph. Tumor oxygen measurements were made under four conditions: (a) normal air breathing, (b) carbogen breathing, (c) after i...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01212813

    authors： Teicher BA,Sotomayor EA,Dupuis NP,Kusumoto T,Menon K

    更新日期：1994-01-01 00:00:00

  • Evidence that a "treatment-free interval of less than 6 months" does not equate with clinically defined platinum resistance in ovarian cancer or primary peritoneal carcinoma.

    abstract：:The standard definition of platinum-resistant ovarian cancer or primary peritoneal carcinoma commonly includes patients whose disease initially responded to a platinum-based combination regimen, but recurred less than 6 months after the completion of primary therapy. Recent experience with several patients with these ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320050177

    authors： Markman M,Kennedy A,Webster K,Kulp B,Peterson G,Belinson J

    更新日期：1998-01-01 00:00:00

  • The age-adjusted Charlson comorbidity index as a predictor of overall survival of surgically treated non-metastatic clear cell renal cell carcinoma.

    abstract：PURPOSE:No study has evaluated the prognostic impact of the age-adjusted Charlson comorbidity index (AACI) in those with renal cell carcinoma (RCC). This study aimed to evaluate the utility of the AACI for predicting long-term survival in patients with surgically treated non-metastatic clear cell RCC (ccRCC). METHODS:...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章,多中心研究

    doi：10.1007/s00432-019-03042-7

    authors： Kang HW,Kim SM,Kim WT,Yun SJ,Lee SC,Kim WJ,Hwang EC,Kang SH,Hong SH,Chung J,Kwon TG,Kim HH,Kwak C,Byun SS,Kim YJ,KORCC (KOrean Renal Cell Carcinoma) Group.

    更新日期：2020-01-01 00:00:00

  • Incidence and risk factors for morbidity and mortality in elderly head and neck cancer patients undergoing major oncological surgery.

    abstract：PURPOSE:Cancer incidence in the elderly population has been continuously rising, and their treatment is an increasing concern among oncologists. This study aimed to evaluate the incidence and risk factors for morbidity and mortality after major oncological surgery in elderly patients with head and neck squamous cell ca...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-016-2141-4

    authors： Yoo SH,Roh JL,Choi SH,Nam SY,Kim SY

    更新日期：2016-06-01 00:00:00

  • Higher cell stiffness indicating lower metastatic potential in B16 melanoma cell variants and in (-)-epigallocatechin gallate-treated cells.

    abstract：PURPOSE:To understand how nanomechanical stiffness affects metastatic potential, we studied the relationship between cell migration, a characteristic of metastasis, and cell stiffness using atomic force microscopy (AFM), which can measure stiffness (elasticity) of individual living cells. METHODS:Migration and cell st...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-012-1159-5

    authors： Watanabe T,Kuramochi H,Takahashi A,Imai K,Katsuta N,Nakayama T,Fujiki H,Suganuma M

    更新日期：2012-05-01 00:00:00

  • Surgery of malignant ovarian tumors.

    abstract：:As currently recognized, the essential aspects of ovarian carcinoma surgery are: (1) the search for occult metastases in tumors located in the lower pelvis, (2) the maximum tumor removal ("tumor-reductive surgery") in advanced ovarian carcinoma and (3) the exploratory laparotomy to confirm a complete clinical remissio...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF00399377

    authors： Almendral AC

    更新日期：1984-01-01 00:00:00

  • Febrile neutropenic events in cancer patients: treatment for fever and neutropenia in young adult patients during intensive chemotherapy for solid tumours.

    abstract：:Febrile neutropenic events (FNE) were studied in 90 patients on chemotherapy protocols for solid tumours, from 1986 to 1990. All patients received intensive chemotherapy with a high dose intensity. There were 51 FNE admissions in 31 patients, with an average event rate of 1.6/patient. The average periods of granulocyt...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01294447

    authors： Nobbenhuis M,Cleton FJ

    更新日期：1992-01-01 00:00:00

  • Prognostic impact of PIK3CA protein expression in triple negative breast cancer and its subtypes.

    abstract：BACKGROUND:Triple negative breast cancer (TNBC) harbors a heterogeneous group of carcinomas with poor prognosis and high genetic variability. As a potential aim for targeted therapy, genetic mutations leading to an activation of the phosphoinositide 3-kinase pathway in a catalytic subunit (PIK3CA) in breast cancer have...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-019-02968-2

    authors： Elfgen C,Reeve K,Moskovszky L,Güth U,Bjelic-Radisic V,Fleisch M,Tausch C,Varga Z

    更新日期：2019-08-01 00:00:00

  • PIK3CA mutation is associated with poor survival among patients with metastatic colorectal cancer following anti-EGFR monoclonal antibody therapy: a meta-analysis.

    abstract：PURPOSE:PIK3CA mutation appears to predict a lack of response to anti-EGFR monoclonal antibody (mAb) treatment in patients with metastatic colorectal cancer (mCRC). However, the predictive value of PIK3CA mutations for survival remains inconclusive. Here, we pooled the data from published studies to estimate the associ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章,meta分析

    doi：10.1007/s00432-013-1400-x

    authors： Wu S,Gan Y,Wang X,Liu J,Li M,Tang Y

    更新日期：2013-05-01 00:00:00

  • Cooperative evaluation of human tumor chemosensitivity in the soft-agar assay and its clinical correlations.

    abstract：:In supporting the human-tumor cloning effort of the Southwest Oncology Group, we conducted an independent retrospective study to evaluate the clinical correlations of the soft-agar colony-forming assay developed by Hamburger and Salmon (1977). This study was made with the cooperation of 76 clinicians and 11 hospitals ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF01884250

    authors： Fan D,Morgan LR,Schneider C,Blank H,Fan S

    更新日期：1985-01-01 00:00:00

  • Mechanisms of membrane damage for CHO cells heated in suspension.

    abstract：:Chinese hamster ovary cells were heated for 20 min at 45.5 degrees C in different conditions, and quantitative determinations of cellular membrane blebbing were performed for cells maintained at 25 degrees C and 37 degrees C after hyperthermia. The percentage of cells with blebs following heating was dependent upon th...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF00390481

    authors： Kapiszewska M,Hopwood LE

    更新日期：1988-01-01 00:00:00

  • Preoperative cervical lymph node size evaluation in patients with malignant head/neck tumors: comparison between ultrasound and computer tomography.

    abstract：PURPOSE:The spread of malignant lymph nodes due to malignancies of the head and neck is systematically observed. However, sentinel lymph nodes in the cervical region, such as in the axillary or supraclavicular regions, are not described. Therefore, precise preoperative lymph node screening of all neck compartments is r...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-008-0487-y

    authors： Hohlweg-Majert B,Metzger MC,Voss PJ,Hölzle F,Wolff KD,Schulze D

    更新日期：2009-06-01 00:00:00

  • Tumor-related elevation of serum (alpha 1----3)-L-fucosyltransferase activity in gastric cancer.

    abstract：:(alpha 1----3)-L-Fucosyltransferase activity was measured in serum samples from 90 gastric cancer patients, 10 patients with benign diseases and 100 healthy controls. The enzyme activity was significantly elevated in the serum samples of patients with cancer compared to those from patients with benign diseases (P less...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF00393336

    authors： Yazawa S,Asao T,Nagamachi Y,Abbas SA,Matta KL

    更新日期：1989-01-01 00:00:00

  • Prognostic impact of pretherapeutic laboratory values in head and neck cancer patients.

    abstract：PURPOSE:The high comorbidity in patients with head and neck cancer (HNC) is mainly caused by the high incidence of tobacco and alcohol abuse and has direct impact on overall survival. We investigated whether HNC and its comorbidity also influence routine laboratory values and whether these values have influence on over...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-012-1320-1

    authors： Peter F,Wittekindt C,Finkensieper M,Kiehntopf M,Guntinas-Lichius O

    更新日期：2013-01-01 00:00:00

  • Kaposiform hemangioendothelioma without cutaneous involvement.

    abstract：PURPOSE:We sought to characterize the clinical features and management of patients diagnosed as Kaposiform hemangioendothelioma (KHE) without cutaneous involvement. METHODS:The electronic patient chats at six Triple A hospitals in China were searched to find all patient diagnoses with KHE without cutaneous involvement...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-018-2759-5

    authors： Ji Y,Chen S,Li L,Yang K,Xia C,Li L,Yang G,Kong F,Lu G,Liu X

    更新日期：2018-12-01 00:00:00

  • Increased expression of cellular retinol-binding protein 1 in laryngeal squamous cell carcinoma.

    abstract：PURPOSE:To investigate the genomic alterations in larynx carcinomas (LaCa) tissues and its prognostics values in predicting survival. METHODS:To analyse the aberrations in the genome of LaCa patients, we used array comparative genomic hybridization in 19 human laryngeal tumour samples. DNA samples were also subjected ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-009-0735-9

    authors： Peralta R,Baudis M,Vazquez G,Juárez S,Ortiz R,Decanini H,Hernandez D,Gallegos F,Valdivia A,Piña P,Salcedo M

    更新日期：2010-06-01 00:00:00

  • Prognostic implication of immunodetection of P glycoprotein in Ewing's sarcoma.

    abstract：:Increased expression of P glycoprotein is associated with multidrug resistance in many cell lines. P glycoprotein has been detected in different human tumors. To assess the implication of multidrug resistance in the prognosis of Ewing's sarcoma the expression of P glycoprotein was studied immunohistochemically in pre-...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01624429

    authors： Roessner A,Ueda Y,Bockhorn-Dworniczak B,Blasius S,Peters A,Wuisman P,Ritter J,Paulussen M,Jürgens H,Böcker W

    更新日期：1993-01-01 00:00:00

  • Modifying effect of reproductive risk factors on the age at onset of breast cancer for German BRCA1 mutation carriers.

    abstract：:Female carriers of mutations in the BRCA1 gene on chromosome 17q have a very high risk of developing breast and/or ovarian cancer during their lifetime. There is, however, little knowledge of to what extent non-genetic risk factors, such as age at menarche, age at first birth, and body mass index, alter the age at ons...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01208638

    authors： Chang-Claude J,Becher H,Eby N,Bastert G,Wahrendorf J,Hamann U

    更新日期：1997-01-01 00:00:00

  • The effect of antibody against vascular endothelial growth factor on tumor growth and metastasis.

    abstract：:Vascular endothelial growth factor (VEGF), a very important in the process of tumor angiogenesis, was chosen as a target in a study to determine whether manipulation of angiogenesis with antibody against VEGF may interrupt tumor growth and metastasis. Anti-VEGF antibody was obtained from immunized rabbits, purified on...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320050223

    authors： Wang G,Dong Z,Xu G,Yang Z,Shou C,Wang N,Liu T

    更新日期：1998-01-01 00:00:00

  • Overexpression of Gs alpha subunit in thyroid tumors bearing a mutated Gs alpha gene.

    abstract：:Point mutations occurring at codon 201 of the gene coding for the alpha subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01366965

    authors： Gorelov VN,Dumon K,Barteneva NS,Palm D,Röher HD,Goretzki PE

    更新日期：1995-01-01 00:00:00

  • Expression of TweakR in breast cancer and preclinical activity of enavatuzumab, a humanized anti-TweakR mAb.

    abstract：BACKGROUND:The receptor for the cytokine TWEAK (TweakR) is a cell surface member of the tumor necrosis factor receptor superfamily with diverse biological roles. TNFRSF family members are appealing therapeutic targets in oncology due to their aberrant expression and function in tumor cells. The goal of the current stud...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s00432-012-1332-x

    authors： Chao DT,Su M,Tanlimco S,Sho M,Choi D,Fox M,Ye S,Hsi ED,Durkin L,Yin J,Zhang Y,Kim H,Starling GC,Culp PA

    更新日期：2013-02-01 00:00:00

  • Alterations in cyclin D1 expression in esophageal squamous cell carcinoma in the Indian population.

    abstract：PURPOSE:The p16/cyclin D1/pRb pathway plays a critical role in tumourigenesis. We recently reported alterations in expression of tumour suppressor gene products, p16 and pRb in esophageal cancer. Knowledge of alterations in cyclin D1, a vital component of this pathway in esophageal carcinomas from the Indian subcontine...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320000185

    authors： Mathew R,Arora S,Khanna R,Shukla NK,Mathur M,Ralhan R

    更新日期：2001-04-01 00:00:00

  • Carcinogenicity of vinylethylnitrosamine in Syrian golden hamsters.

    abstract：:Syrian golden hamsters received weekly s.c. doses of 5.0, 2.5, 1.25 or 0.63 mg/kg b.w. vinylethylnitrosamine (VEN). As dose levels increased, average survival time decreased, tumor multiplicity increased as did the total tumor rate which was between 50% and 100%. The main target of VEN effect was the respiratory tract...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF00411342

    authors： Green U,Althoff J

    更新日期：1982-01-01 00:00:00

  • Smoking and mortalities from cancer, coronary heart disease and stroke in male Japanese physicians.

    abstract：:A cohort of 5,477 male Japanese physicians was studied to examine the relationship between smoking habits and mortalities from cancer, coronary heart disease (CHD) and stroke over 12.7 years. The logistic regression analysis based on proportional hazard models was used for statistical assessment. The risks of both lun...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF00402732

    authors： Kono S,Ikeda M,Tokudome S,Nishizumi M,Kuratsune M

    更新日期：1985-01-01 00:00:00

  • Influence of recombinant tumour necrosis factor alpha on blood flow and antibody localisation in human tumour xenografts in nude mice.

    abstract：:Mice with s.c. grafts of gastric carcinoma MKN45 or osteosarcoma 788T were injected i.v. with recombinant tumour necrosis factor alpha (rTNF alpha) and tumour blood flow rates were determined 4 h later as a fraction of the cardiac output g tissue-1. With MKN45, the tumour blood flow rate was significantly reduced from...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/BF01613286

    authors： Pimm MV,Gribben SJ,Morris TM

    更新日期：1991-01-01 00:00:00

  • Cytotoxicity, cell-cycle perturbations and apoptosis in human tumor cells by lipophilic N4-alkyl-1-beta-D-arabinofuranosylcytosine derivatives and the new heteronucleoside phosphate dimer arabinocytidylyl-(5'-->5')-N4-octadecyl-1-beta-D-arabinofuranosylcy

    abstract：:The arabinofuranosylcytosine (AraC) derivative N4-octadecyl-1-beta-D-arabinofuranosylcytosine (NOAC) and its (5'-->5')-heterodinucleoside phosphate analog NOAC-AraC were compared with AraC for cytotoxicity, cell-cycle dependence, phosphorylation by deoxycytidine (dC) kinase and apoptosis induction in native, AraC- or ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320050349

    authors： Horber DH,Cattaneo-Pangrazzi RM,von Ballmoos P,Schott H,Ludwig PS,Eriksson S,Fichtner I,Schwendener RA

    更新日期：2000-06-01 00:00:00

  • Combined therapies for cancer: a review of EGFR-targeted monotherapy and combination treatment with other drugs.

    abstract：:Targeted therapy refers to anticancer treatment which specifically targets key molecules of cancer cells and/or neovascular cells, aiming to thus interfere with processes of tumorigenesis, cancer progression and metastasis. The epidermal growth factor receptor (EGFR) was the first receptor to be proposed for targeted ...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章,评审

    doi：10.1007/s00432-009-0622-4

    authors： Zahorowska B,Crowe PJ,Yang JL

    更新日期：2009-09-01 00:00:00

  • The effect of pyrimidine nucleosides on adenosine-induced apoptosis in HL-60 cells.

    abstract：:Among several nucleosides, adenosine is the only one to induce typical apoptotic cell death in human promyelocytic leukemia HL-60 cells. Intracellularly transported adenosine seemed to be required for the induction of apoptosis, since dipyridamole, which inhibits the transport of adenosine, strongly suppressed apoptos...

    journal_title：Journal of cancer research and clinical oncology

    pub_type： 杂志文章

    doi：10.1007/s004320050201

    authors： Kim KT,Yeo EJ,Choi H,Park SC

    更新日期：1998-01-01 00:00:00