Abstract：
:Chinese hamster ovary cells were heated for 20 min at 45.5 degrees C in different conditions, and quantitative determinations of cellular membrane blebbing were performed for cells maintained at 25 degrees C and 37 degrees C after hyperthermia. The percentage of cells with blebs following heating was dependent upon the composition of the medium during heating and the posthyperthermia temperature after heating. The total extent of bleb formation after heating was independent of the calcium-ion concentration in the medium during heating; however, differences in the kinetics of bleb disappearance after heating point to the importance of Ca2+ concentration in the expression of heat damage. Without hyperthermia, blebs were formed on the cell-surface membrane with agents which block sulfhydryl groups or release calcium from cellular stores. The cells were protected from bleb formation when cells were incubated with glutathione before addition of sulfhydryl-blocking agents or heat treatment. Oligomycin did not prevent the formation of blebs, suggesting that this phenomenon is not energy-dependent. Only a small percentage of cells were covered with blebs when they were heated in saline solution. When cells were incubated with dbcAMP before heat, blebs did not appear at 25 degrees C. A possible interpretation for these observations is presented.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Kapiszewska M,Hopwood LEdoi
10.1007/BF00390481subject
Has Abstractpub_date
1988-01-01 00:00:00pages
23-9issue
1eissn
0171-5216issn
1432-1335journal_volume
114pub_type
杂志文章abstract：:The antitumor and anti-metastatic effects of a potent angiogenesis inhibitor, O-(chloroacetyl-carbamoyl)fumagillol (TNP-470), was investigated in a highly metastatic model of human hepatocellular carcinoma-LCI-D20. Small pieces of LCI-D20 tumor tissue were implanted subcutaneously into the right axillary region of 24 ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01240121
更新日期：1997-01-01 00:00:00
abstract：:Female wistar rats were fed on an Mg-deficient diet. After 8 to 10 weeks, the thymus glands were strongly degenerated and the number of lymphocytes was reduced, especially in the cortex. Thymus degeneration was associated with a decreased rate of DNA, RNA, and protein biosynthesis and necrosis and phagocytosis of lymp...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00402054
更新日期：1982-01-01 00:00:00
abstract：:Passively administered and actively induced antibodies have been associated with the eradication of circulating tumor cells and micrometastases in mice and humans. We have identified a series of cell surface carbohydrate and peptide antigens on melanomas, sarcomas, and cancer of the breast, prostate. ovary, and lung t...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01470995
更新日期：2001-10-01 00:00:00
abstract：PURPOSE:The objective was to compare the prognostic factors and outcomes among primary ovarian cancer (OC), fallopian tube cancer (FC), and peritoneal cancer (PC) patients in a population-based setting. METHODS:We analysed 5399 OC, 327 FC, and 416 PC patients diagnosed between 1998 and 2014 in the catchment area of th...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-017-2422-6
更新日期：2017-09-01 00:00:00
abstract：:Three thousand Yoshida sarcoma cells were inoculated into the wall of the descending colon of each of 120 male Sprague-Dawley rats. On day 8 after the tumor implantation, the animals were at random divided into four groups of 30 rats each. The effect of cyclophosphamide (70 mg/kg), BCNU (25 mg/kg), and methyl-CCNU (45...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00405350
更新日期：1979-05-14 00:00:00
abstract：OBJECTIVE:To evaluate through retrospective analysis the efficacy and toxicity of combination chemotherapy with etoposide, vincristine, doxorubin and dexamethasone (EVAD) as second-line therapy in patients with advanced AIDS-related Kaposi's sarcoma (AIDS-KS) after failure of first-line chemotherapy. METHODS:Eighty-ei...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-011-1109-7
更新日期：2012-03-01 00:00:00
abstract：PURPOSE:The primary cause of local recurrence and therapeutic failure in the treatment of malignant gliomas is the invasion of tumor cells into the surrounding normal brain. While it is known that malignant gliomas infiltrate diffusely into regions of normal brain, it is frequently very difficult to unequivocally ident...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-002-0366-x
更新日期：2002-09-01 00:00:00
abstract：:The effect of growth phase on enzymatic activities of the de novo and salvage pathways for purine and pyrimidine nucleotide synthesis was studied in a hepatocyte-derived cell line from the rat. The cells were in lag phase after plating for 36 h; log phase started at 48 h and persisted up to 120 h of culture. Then the ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01612899
更新日期：1990-01-01 00:00:00
abstract：PURPOSE:Our primary goal was to determine whether increased expression of the transmembrane carbonic anhydrase (CA) isozymes in renal cell carcinoma (RCC) is regulated by the von Hippel-Lindau (VHL) gene. METHODS:We studied mRNA expression of all three transmembrane CAs - CA IX, XII, and XIV - in 17 RCC cell lines, tr...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-002-0374-x
更新日期：2002-10-01 00:00:00
abstract：:Indirubin, a 3, 2' bisindole isomer of indigo, has originally been identified as the active principle of a traditional Chinese preparation and has been proven to exhibit antileukemic effectiveness in chronic myelocytic leukemia. Indirubin was detected to represent a novel lead structure with potent inhibitory potentia...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-004-0579-2
更新日期：2004-11-01 00:00:00
abstract：:159 "poor risk" patients with previously untreated small cell lung cancer were entered into a randomised study of intravenous bolus versus continuous infusion chemotherapy with doxorubicin on weeks 1, 3, 5 and ifosfamide with mesna on weeks 2, 4, 6. Oral etoposide was given on days 1-5, 15-19, and 29-33. There was no ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1007/BF01613219
更新日期：1991-01-01 00:00:00
abstract：:In addition to spontaneous uterine endometrial adenocarcinomas at a high incidence (35.1%), development of endometrial hyperplasia/adenoma was also frequently detected in rats of the Donryu strain. The total yield of all observed proliferative endometrial lesions was very high (60.6%). The tumors arose commonly in the...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01637084
更新日期：1990-01-01 00:00:00
abstract：PURPOSE:The aim of this study was to assess the impact of cyclin D1 overexpression (considered separately or jointly with previously assessed p53 and pRb statuses) on survival in a group of 111 surgically treated non-small cell lung cancer patients (NSCLC). METHODS:Cyclin D1 accumulation was assessed immunohistochemic...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-004-0661-9
更新日期：2005-07-01 00:00:00
abstract：:Concentrations of a fragment of the c-erbB-2 translational product (p185 fragment) were measured in serum of 70 breast cancer patients, 19 healthy blood donors, and 18 pregnant women using a heterogenic enzyme immunoassay. The serum concentrations of blood donors and pregnant women were below 30 kU/l. Breast cancer pa...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01212719
更新日期：1993-01-01 00:00:00
abstract：PURPOSE:In 2008, we reported that 10 Japanese-size cups of green tea daily, supplemented with tablets of green tea extract (GTE), reduced the recurrence of colorectal adenoma by 51.6% in patients after polypectomy. Based on these results, we paid special attention to Japanese cancer patients, who consume green tea ever...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/s00432-014-1899-5
更新日期：2015-09-01 00:00:00
abstract：OBJECTIVES:Solitary plasmacytoma (SP) is characterized by a single mass of clonal plasma cells. Definitive RT can result in long-term local control of the SP. Due to the small number of patients and narrow range of doses, phase III randomized trials are lacking. The aim of this study is to further support the potential...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03452-y
更新日期：2020-11-17 00:00:00
abstract：PURPOSE:Human papillomavirus (HPV) type 16 and 18 are the most prevalent genotypes in cervical cancers. The viral oncoproteins E6 and E7 are considered to be tumor-specific targets for immunotherapy. HPV E7 antigen-loaded dendritic cells (DC) were evaluated as cellular tumor vaccine. METHODS:Autologous monocyte-derive...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-003-0462-6
更新日期：2003-09-01 00:00:00
abstract：:To verify whether a mild, but prolonged liver injury by chemicals needing bioactivation causes both hepatic cirrhosis and the appearance of hepatocyte nodules and tumors (providing the liver has been exposed previously to initiating stimuli), diethylnitrosamine-initiated and uninitiated rats were administered thioacet...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00391596
更新日期：1989-01-01 00:00:00
abstract：:The hospital records of 60 female and 56 male pancreatic cancer patients and 232 age-, race-, sex-, and hospital-matched controls were reviewed for evidence of prior thyroid disease, cholecystectomy, appendectomy, alcohol consumption and among females, for diseases for the endometrium and ovary. No statistically signi...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00410309
更新日期：1982-01-01 00:00:00
abstract：OBJECTIVE:In recurrent ovarian cancer (ROC), there is a high demand on effective therapies with a mild toxicity profile. Treosulfan is an alkylating agent approved as oral (p.o.) and intravenous (i.v.) formulation for the treatment of recurrent ovarian cancer. Data on safety and efficacy for either formulation are rare...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1007/s00432-016-2307-0
更新日期：2017-03-01 00:00:00
abstract：BACKGROUND:Recent advances in sequencing technologies supported the development of molecularly targeted therapy in cancer patients. Thus, genomic analyses are becoming a routine part in clinical practice and accurate detection of actionable mutations is essential to assist diagnosis and therapy choice. However, this is...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-017-2399-1
更新日期：2017-07-01 00:00:00
abstract：OBJECTIVES:Evidence from multiple clinical trials showed that local consolidative therapy (LCT) improved survival in oligometastatic non-small cell lung cancer (NSCLC) patients. In the present study, we aim to explore the potential role of microwave ablation (MWA) as LCT for epidermal growth factor receptor (EGFR)-muta...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-019-03043-6
更新日期：2020-01-01 00:00:00
abstract：PURPOSE:Since continuous relapse rates are characteristic for advanced low-grade non-Hodgkin's lymphomas (NHL), their treatment remains a problem. Previous phase II studies showed that bendamustine induced high remission rates in pre-treated low-grade NHL. To further examine its efficacy and side effects, bendamustine ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 临床试验,杂志文章
doi：10.1007/s00432-002-0378-6
更新日期：2002-11-01 00:00:00
abstract：:The effect of a single blood transfusion on the formation and outgrowth of experimental lung metastases was assessed in two tumor models in rats. The transfusions were given either 1 week before (day -7) or 1 week after (day +7) tumor cell inoculation. The first approach was performed to investigate the effect of tran...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00402776
更新日期：1986-01-01 00:00:00
abstract：:Hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP) are markers of collagen absorption and LP is specific for collagen type I in bone. In the present study we evaluated the concentration of HP and LP in urine of patients with osteosarcoma ( n=20; age range 16-49 years) and chondrosarcoma ( n=15; age range 18-70 y...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-003-0470-6
更新日期：2003-10-01 00:00:00
abstract：PURPOSE:The aim of this study was to investigate the prognostic significance of the absolute natural killer (NK) cell counts in peripheral blood in patients with chronic lymphocytic leukemia (CLL). METHODS:A total of 273 previously untreated patients with CLL from April 2004 and October 2015 were enrolled into this re...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-017-2568-2
更新日期：2018-03-01 00:00:00
abstract：:Although the biological action of phthalates has been widely discussed there is little information on early cellular changes indicative for toxic or carcinogenic effects. To study subtle alterations in the cell morphology, we have by means of image processing evaluated the nuclei of hamster hepatocytes after treatment...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00400745
更新日期：1986-01-01 00:00:00
abstract：BACKGROUND:In the phase 3 BFORE trial (NCT02130557), treatment with bosutinib resulted in a significantly higher major molecular response rate at 12 months versus imatinib in the modified intent-to-treat (mITT) population of patients with newly diagnosed chronic phase chronic myeloid leukemia (CP CML). Assessment of pa...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,随机对照试验
doi：10.1007/s00432-019-02894-3
更新日期：2019-06-01 00:00:00
abstract：:Female carriers of mutations in the BRCA1 gene on chromosome 17q have a very high risk of developing breast and/or ovarian cancer during their lifetime. There is, however, little knowledge of to what extent non-genetic risk factors, such as age at menarche, age at first birth, and body mass index, alter the age at ons...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01208638
更新日期：1997-01-01 00:00:00
abstract：:The carcinogenic effectiveness of nitrosomethyl-n-butylamine,-n-hexylamine and -n-heptylamine was compared by administration to F344 rats at various concentrations in drinking water and by gavage in oil. Nitrosomethylbutylamine induced only tumors of the upper gastrointestinal tract, and was very toxic. Nitrosomethylh...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00402603
更新日期：1983-01-01 00:00:00
