Enhanced RNA binding of dimerized aminoglycosides.

Abstract:

:Aminoglycoside antibiotics have recently emerged as an intriguing family of RNA binding molecules and they became leading structures for the design of novel RNA ligands. The demystification of the aminoglycoside-RNA recognition phenomenon is required for the development of superior binders. To explore the existence of multiple binding sites in a large RNA molecule, we have synthesized covalently linked symmetrical and nonsymmetrical dimeric aminoglycosides. These unnatural derivatives were compared to their natural "monomeric" counterparts in their ability to inhibit the Tetrahymena ribozyme. The dimeric aminoglycosides inhibit ribozyme function 20 to 1.2 x 10(3) fold more effectively than their natural parent compounds. The inhibition curves of dimeric aminoglycosides have characteristic shapes suggesting the presence of at least two high affinity-binding sites within the ribozyme's three-dimensional fold. The interaction of a dimeric aminoglycoside with two complementary sites of the RNA molecule is proposed. This binding motif may have implications on the development of new drugs targeting pivotal RNA molecules of bacterial and viral pathogens.

journal_name

Bioorg Med Chem

authors

Michael K,Wang H,Tor Y

doi

10.1016/s0968-0896(99)00071-1

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

1361-71

issue

7

eissn

0968-0896

issn

1464-3391

pii

S0968-0896(99)00071-1

journal_volume

7

pub_type

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