Oligomerization of activated D- and L-guanosine mononucleotides on templates containing D- and L-deoxycytidylate residues.

abstract：:The effects of urea and of guanidinium chloride on binding constants in water for 6-(4-tert-butylanilino)-naphthalene-2-sulfonate and of bis(p-tert-butylphenyl) phosphate binding to beta-cyclodextrin and to N,N'-bis(6-beta-cyclo-dextrinyl)imidazolium ion have been determined. Their effects on the water solubility of p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Breslow R,Halfon S

更新日期：1992-08-01 00:00:00

abstract：:The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted. Dynamin is a GTPase shown to play an important role in the control clathrin mediated endocytosis of EGFR and other cell surface receptors. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sousa LP,Lax I,Shen H,Ferguson SM,De Camilli P,Schlessinger J

更新日期：2012-03-20 00:00:00

abstract：:Human appropriation of net primary production (HANPP), the aggregate impact of land use on biomass available each year in ecosystems, is a prominent measure of the human domination of the biosphere. We present a comprehensive assessment of global HANPP based on vegetation modeling, agricultural and forestry statistics...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Haberl H,Erb KH,Krausmann F,Gaube V,Bondeau A,Plutzar C,Gingrich S,Lucht W,Fischer-Kowalski M

更新日期：2007-07-31 00:00:00

abstract：:The neonatal Fc receptor for IgG (FcRn) is a distant member of the MHC class I protein family. It binds IgG and albumin in a pH-dependent manner and protects these from catabolism by diverting them from a degradative fate in lysosomes. In addition, FcRn-mediated IgG transport across epithelial barriers is responsible ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiao SW,Kobayashi K,Johansen FE,Sollid LM,Andersen JT,Milford E,Roopenian DC,Lencer WI,Blumberg RS

更新日期：2008-07-08 00:00:00

abstract：:When either the homologous RNA (avian myeloblastosis virus RNA) or a heterologous RNA (poliovirus RNA) was used as a template, the anticomplementary DNA synthesized in vitro by avian myeloblastosis virus reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) was primed by fragments of the original...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Myers JC,Dobkin C,Spiegelman S

更新日期：1980-03-01 00:00:00

abstract：:The interaction between the ribosomal-stalk protein L7/12 (L12) and initiation factor 2 (IF2) is essential for rapid subunit association, but the underlying mechanism is unknown. Here, we have characterized the L12-IF2 interaction on Escherichia coli ribosomes using site-directed mutagenesis, fast kinetics, and molecu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ge X,Mandava CS,Lind C,Åqvist J,Sanyal S

更新日期：2018-05-01 00:00:00

abstract：:The dynamic responses of the hearing organ to acoustic overstimulation were investigated using the guinea pig isolated temporal bone preparation. The organ was loaded with the fluorescent Ca2+ indicator Fluo-3, and the cochlear electric responses to low-level tones were recorded through a microelectrode in the scala m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fridberger A,Flock A,Ulfendahl M,Flock B

更新日期：1998-06-09 00:00:00

abstract：:In addition to their roles in desensitization and signaling of seven-membrane-spanning receptors, beta-arrestins have been more recently implicated in regulating non-seven-membrane-spanning receptor pathways. By using a yeast two-hybrid screen, we identified the inhibitor of NF-kappaB, IkappaBalpha, as a binding partn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Witherow DS,Garrison TR,Miller WE,Lefkowitz RJ

更新日期：2004-06-08 00:00:00

abstract：:The primary structure of the beta chain of human glycoprotein Ib (GPIb), the platelet receptor for von Willebrand factor, has been established by a combination of cDNA cloning and amino acid sequence analysis. A lambda phage cDNA expression library prepared from human erythroleukemia cells (HEL cells) was screened wit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez JA,Chung DW,Fujikawa K,Hagen FS,Davie EW,Roth GJ

更新日期：1988-04-01 00:00:00

abstract：:Alzheimer's disease (AD) and other neurodegenerative disorders are associated with the cytoplasmic aggregation of microtubule-associated protein tau. Recent evidence supports transcellular transfer of tau misfolding (seeding) as the mechanism of spread within an affected brain, a process reminiscent of viral infection...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McEwan WA,Falcon B,Vaysburd M,Clift D,Oblak AL,Ghetti B,Goedert M,James LC

更新日期：2017-01-17 00:00:00

abstract：:The Raf-1 serine/threonine kinase is a key protein involved in the transmission of many growth and developmental signals. In this report, we show that autoinhibition mediated by the noncatalytic, N-terminal regulatory region of Raf-1 is an important mechanism regulating Raf-1 function. The inhibition of the regulatory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cutler RE Jr,Stephens RM,Saracino MR,Morrison DK

更新日期：1998-08-04 00:00:00

abstract：:Faithful transmission of genetic material to daughter cells involves a characteristic temporal order of DNA replication, which may play a significant role in the inheritance of epigenetic states. We developed a genome-scale approach--Repli Seq--to map temporally ordered replicating DNA using massively parallel sequenc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hansen RS,Thomas S,Sandstrom R,Canfield TK,Thurman RE,Weaver M,Dorschner MO,Gartler SM,Stamatoyannopoulos JA

更新日期：2010-01-05 00:00:00

abstract：:Whether the regulatory protein of an inducible operon for a catabolic pathway is a positive or a negative element in the control of expression is correlated with the frequent or infrequent presence, respectively, of the system's substrate in the natural environment. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Savageau MA

更新日期：1974-06-01 00:00:00

abstract：:The E6 oncoprotein of human papillomaviruses (HPVs) that are associated with cervical cancer utilizes the cellular ubiquitin-protein ligase E6-AP to target the tumor suppressor p53 for degradation. In normal cells (i.e., in the absence of E6), p53 is also a target of the ubiquitin-proteasome pathway. Under these condi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hengstermann A,Linares LK,Ciechanover A,Whitaker NJ,Scheffner M

更新日期：2001-01-30 00:00:00

abstract：:Among the multiple steps constituting the kinesin mechanochemical cycle, one of the most interesting events is observed when kinesins move an 8-nm step from one microtubule (MT)-binding site to another. The stepping motion that occurs within a relatively short time scale ( approximately 100 mus) is, however, beyond th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyeon C,Onuchic JN

更新日期：2007-10-30 00:00:00

abstract：:Better understanding myelination of peripheral nerves would benefit patients affected by peripheral neuropathies, including Charcot-Marie-Tooth disease. Little is known about the role the Golgi compartment plays in Schwann cell (SC) functions. Here, we studied the role of Golgi in myelination of peripheral nerves in m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baba T,Alvarez-Prats A,Kim YJ,Abebe D,Wilson S,Aldworth Z,Stopfer MA,Heuser J,Balla T

更新日期：2020-11-10 00:00:00

abstract：:Field cancerization predisposes the upper aerodigestive tract mucosa to the formation of multiple primary tumors, when exposed to environmental carcinogens. Up-regulation of epidermal growth factor receptor occurs early in squamous cell carcinogenesis and is critical for the loss of growth control in a variety of huma...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grandis JR,Drenning SD,Zeng Q,Watkins SC,Melhem MF,Endo S,Johnson DE,Huang L,He Y,Kim JD

更新日期：2000-04-11 00:00:00

abstract：:G-protein-coupled receptors (GPCRs) have recently joined the list of cell surface receptors that dimerize. Dimerization has been shown to alter the ligand-binding, signaling, and trafficking properties of these receptors. Recent studies have shown that GPCRs heterodimerize with closely related members, resulting in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jordan BA,Trapaidze N,Gomes I,Nivarthi R,Devi LA

更新日期：2001-01-02 00:00:00

abstract：:Capsaicin (CP), the pungent component of chili pepper, acts on sensory neurons to convey the sensation of pain. The CP receptor, vanilloid receptor 1 (VR1), has been shown to be highly expressed by nociceptive neurons in dorsal root and trigeminal ganglia. We demonstrate here that the dendritic cell (DC), a key cell t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Basu S,Srivastava P

更新日期：2005-04-05 00:00:00

abstract：:After prolonged visual adaptation of Drosophila, dramatic long-term changes of in vitro phosphorylation of a 50-kDa brain protein that is identical to the Ca2+/calmodulin-dependent protein kinase (EC 2.7.1.37) can be measured in isolated heads. By selective receptor cell desensitization in blue light, subcellular dist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willmund R,Mitschulat H,Schneider K

更新日期：1986-12-01 00:00:00

abstract：:Genetic switches based on the [Formula: see text] system are master regulators of an array of cellular responses. Recent kinetic experiments have shown that [Formula: see text] can actively remove NF-κB bound to its genetic sites via a process called "molecular stripping." This allows the [Formula: see text] switch to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Potoyan DA,Zheng W,Komives EA,Wolynes PG

更新日期：2016-01-05 00:00:00

abstract：:An RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) has been purified from phage-SP01-infected Bacillus subtilis that copies RNA almost exclusively from the heavy strand of native SP01 DNA, the DNA strand from which "middle" and "late" classes of RNA are copied in vivo. Hybridization-com...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pero J,Nelson J,Fox TD

更新日期：1975-04-01 00:00:00

abstract：:Central nervous system (CNS) trauma can result in tissue disruption, neuronal and axonal degeneration, and neurological dysfunction. The limited spontaneous CNS repair in adulthood and aging is often insufficient to overcome disability. Several investigations have demonstrated that targeting HDAC activity can protect ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rivieccio MA,Brochier C,Willis DE,Walker BA,D'Annibale MA,McLaughlin K,Siddiq A,Kozikowski AP,Jaffrey SR,Twiss JL,Ratan RR,Langley B

更新日期：2009-11-17 00:00:00

abstract：:Three proteins required for poly(U)-directed polyphenylalanine synthesis have been separated from yeast. Two of the factors correspond to the elongation factors 1 and 2 described for other eukaryotic systems, according to the criteria of phenylalanyl-tRNA binding and diphtheria toxin-catalyzed ADP-ribosylation. The th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skogerson L,Wakatama E

更新日期：1976-01-01 00:00:00

abstract：:In autotrophically grown Chlorella cells, glucose induces a hexose transport system but, at the same time, the synthesis of two amino acid transport systems is also induced. Thus, the rates of uptake of glycine, L-alanine, L-proline, and L-serine, all of which compete with each other for entry into the cells, increase...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cho BH,Sauer N,Komor E,Tanner W

更新日期：1981-06-01 00:00:00

abstract：:The antigenic mosaic of the Archaebacterium Methanobacterium thermoautotrophicum, strain delta H, was analyzed with a panel of six monoclonal antibodies. Five antigenic determinants were identified. One contains N-acetyl-D-glucosamine, another contains N-acetyl-D-galactosamine, and a third contains gamma-glutamylalani...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Conway de Macario E,Macario AJ,Magariños MC,König H,Kandler O

更新日期：1983-10-01 00:00:00

abstract：:Graphene superlattices were shown to exhibit high-temperature quantum oscillations due to periodic emergence of delocalized Bloch states in high magnetic fields such that unit fractions of the flux quantum pierce a superlattice unit cell. Under these conditions, semiclassical electron trajectories become straight agai...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Krishna Kumar R,Mishchenko A,Chen X,Pezzini S,Auton GH,Ponomarenko LA,Zeitler U,Eaves L,Fal'ko VI,Geim AK

更新日期：2018-05-15 00:00:00

abstract：:The triphenylmethane derivative aurintricarboxylic acid (ATA), but not aurin, selectively prevented the binding of OKT4A/Leu-3a monoclonal antibody (mAb) and, to a lesser extent, OKT4 mAb to the CD4 cell receptor for human immunodeficiency virus type 1 (HIV-1). The effect was seen within 1 min at an ATA concentration ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schols D,Baba M,Pauwels R,Desmyter J,De Clercq E

更新日期：1989-05-01 00:00:00

abstract：:Retinal dopaminergic amacrine neurons (DA neurons) play a central role in reconfiguring retinal function according to prevailing illumination conditions, yet the mechanisms by which light regulates their activity are poorly understood. We investigated the means by which sustained light responses are evoked in DA neuro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang DQ,Wong KY,Sollars PJ,Berson DM,Pickard GE,McMahon DG

更新日期：2008-09-16 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) thymidine kinase is currently used as a suicide agent in the gene therapy of cancer. This therapy is based on the preferential phosphorylation of nucleoside analogs by tumor cells expressing HSV-1 thymidine kinase. However, the use of HSV-1 thymidine kinase is limited in part by the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Black ME,Newcomb TG,Wilson HM,Loeb LA

更新日期：1996-04-16 00:00:00