Effects of an inducible anti-sense c-myc gene transfer in a drug-resistant human small-cell-lung-carcinoma cell line.


:Small-cell-lung-cancer (SCLC) is characterized by rapid development of resistance to cytotoxic agents, such as cisplatin (cDDP) and anthracyclines. c-myc over-expression is one of the reported genetic alterations in this tumor. Amplification of the c-myc gene in this and other cancers is often correlated with poor prognosis. We studied the existence of a correlation between resistance and activation of the c-myc oncogene in a cDDP-resistant SCLC sub-line, GLC4cDDP, containing a c-myc gene amplification. This cell line was stably transfected with either an anti-sense c-myc (AS) or control (C) expression vector resulting in the sub-clones GLC4cDDP/AS and GLC4cDDP/C respectively. PCR and RT-PCR analysis illustrated the integration and activation of the dexamethasone(dex)-inducible MMTV-LTR promoter linked to the complete AS-c-myc fragment in GLC4cDDP/AS cells, but not in GLC4cDDP/C cells. Dex-induced AS-c-myc RNA resulted in 50% growth inhibition during the first 48 hr, which declined to 25% at 72 hr. In addition, AS-c-myc RNA expression reduced the cloning efficacy by 36% and induced 2-fold more apoptosis within 24 hr in GLC4cDDP/AS cells. Dex treatment did not affect the proliferation, clonogenicity and induction of apoptosis in the control cell lines. Furthermore, AS-c-myc RNA expression caused a 1.4-fold increased cDDP sensitivity but no change in doxorubicin or vincristine sensitivity in GLC4cDDP/AS cells. Our results indicate that AS-c-myc RNA expression causes inhibition of cell proliferation, induces apoptosis, reduces clonogenicity and interferes with cDDP sensitivity but not with doxorubicin or vincristine sensitivity.


Int J Cancer


Van Waardenburg RC,Meijer C,Burger H,Nooter K,De Vries EG,Mulder NH,De Jong S




Has Abstract


1997-11-14 00:00:00














  • Correlation between cytogenetic data and ganglioside pattern in human meningiomas.

    abstract::Partial or total loss of chromosome 22 is often associated with tumors of the central nervous system and in particular with meningiomas. As in the case of other tumors, the ganglioside pattern is modified in transformed tissues. Cytogenetic analysis of 30 human meningiomas has been performed and the results compared t...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Berra B,Papi L,Bigozzi U,Serino D,Morichi R,Mennonna P,Rapelli S,Cogliati T,Montali E

    更新日期:1991-02-01 00:00:00

  • Interleukin-10 expression and cytotoxic-T-cell response in Epstein-Barr-virus-associated nasopharyngeal carcinoma.

    abstract::Interleukin-10 (IL-10) is an inhibitory cytokine produced by various cell types. It exhibits strong sequence homology to BCRF-1 (viral IL-10, vIL-10), an open reading frame in the Epstein-Barr-virus (EBV) genome. Using in situ hybridization (ISH), polymerase chain reaction (PCR) and immunohistochemistry, we checked 41...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Yao M,Ohshima K,Suzumiya J,Kume T,Shiroshita T,Kikuchi M

    更新日期:1997-07-29 00:00:00

  • Biological effects induced by insulin-like growth factor binding protein 3 (IGFBP-3) in malignant melanoma.

    abstract::The insulin like growth factor (IGF) signaling pathway has been shown to contribute to melanoma progression, but little is known about the role of the IGF binding protein 3 (IGFBP-3) in melanoma biology. The aim of the present study was to characterize expression, function and regulation of IGFBP-3 in malignant melano...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Oy GF,Slipicevic A,Davidson B,Solberg Faye R,Maelandsmo GM,Flørenes VA

    更新日期:2010-01-15 00:00:00

  • Sphingosine kinase 2 deficient tumor xenografts show impaired growth and fail to polarize macrophages towards an anti-inflammatory phenotype.

    abstract::A challenging task of the immune system is to fight cancer cells. However, a variety of human cancers educate immune cells to become tumor supportive. This is exemplified for tumor-associated macrophages (TAMs), which are polarized towards an anti-inflammatory and cancer promoting phenotype. Mechanistic explanations, ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Weigert A,Schiffmann S,Sekar D,Ley S,Menrad H,Werno C,Grosch S,Geisslinger G,Brüne B

    更新日期:2009-11-01 00:00:00

  • Ganglioside GM2 expression on human melanoma cells correlates with sensitivity to lymphokine-activated killer cells.

    abstract::Ganglioside GM2 is expressed on cell surface membranes of a variety of human malignant cells and has been demonstrated to be immunogenic in humans. We have assessed the role of the antigen GM2 on melanoma cells as a recognition structure for lymphokine-activated killer (LAK) cells. LAK cells were generated by stimulat...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hoon DS,Ando I,Sviland G,Tsuchida T,Okun E,Morton DL,Irie RF

    更新日期:1989-05-15 00:00:00

  • Risks of second primary malignancy in patients with cutaneous and ocular melanoma in Denmark, 1943-1989.

    abstract::Risks of 2nd primary cancer were assessed in all patients with cutaneous melanoma (12,460) and all patients with ocular melanoma (2,018) incident in Denmark from 1943 to 1989 and followed for totals of 88,667 person-years and 16,045 person-years, respectively. After cutaneous melanoma, 972 2nd cancers occurred. The ri...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Swerdlow AJ,Storm HH,Sasieni PD

    更新日期:1995-06-09 00:00:00

  • Evidence for alternative lengthening of telomeres in liposarcomas in the absence of ALT-associated PML bodies.

    abstract::Immortalized and cancer cells maintain their telomeres by activation of a telomere maintenance mechanism (TMM). In approximately 85% of cancers telomerase is activated (TA) but in some tumours, in particular sarcomas, an alternative lengthening of telomeres (ALT) pathway is used. Liposarcomas are the most common soft-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Jeyapalan JN,Mendez-Bermudez A,Zaffaroni N,Dubrova YE,Royle NJ

    更新日期:2008-06-01 00:00:00

  • The risk of brain tumours in hereditary non-polyposis colorectal cancer (HNPCC).

    abstract::Hereditary non-polyposis colorectal cancer (HNPCC) is known to be associated with several extracolonic cancers, e.g., cancers of the endometrium, stomach, urinary tract, small bowel and ovary. An association between HNPCC and brain tumours has also been reported, although previous risk analysis did not reveal an exces...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Vasen HF,Sanders EA,Taal BG,Nagengast FM,Griffioen G,Menko FH,Kleibeuker JH,Houwing-Duistermaat JJ,Meera Khan P

    更新日期:1996-02-08 00:00:00

  • Functional variant of MTOR rs2536 and survival of Chinese gastric cancer patients.

    abstract::We previously reported that some single nucleotide polymorphisms (SNPs) of candidate genes involved in the MTOR complex1 (MTORC1) were associated with risk of gastric cancer (GCa). In the present study, we further evaluated associations of eight potentially functional SNPs of MTOR, MLST8 and RPTOR with survival of 100...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Cheng L,Qiu L,Zhang R,Qian D,Wang M,Sun M,Zhu X,Wang Y,Guo W,Wei Q

    更新日期:2019-01-15 00:00:00

  • Comparison of the metastasis-inducing protein S100A4 (p9ka) with other prognostic markers in human breast cancer.

    abstract::Our aim was to compare the occurrence and prognostic significance over 14-20 years of immunocytochemically detected S100A4 and other tumour variables in primary tumours from 349 patients with operable breast cancer. For a cut-off of 1% staining of the malignant cells, the antibody to S100A4 stains positively 56% of th...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Platt-Higgins AM,Renshaw CA,West CR,Winstanley JH,De Silva Rudland S,Barraclough R,Rudland PS

    更新日期:2000-03-20 00:00:00

  • Systemic and in situ natural killer and suppressor cell activities in mice bearing progressively growing murine sarcoma-virus-induced tumors.

    abstract::The activity of systemic and in situ natural killer (NK) cells was measured in 3- to 5-week-old CBA mice with progressively growing murine sarcoma virus (MSV)-induced tumors. Splenic NK activity was depressed in tumor-bearing mice. NK activity was quite low or not detectable in unfractionated cell suspensions from the...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gerson J,Varesio L,Herberman RB

    更新日期:1981-02-15 00:00:00

  • Incidence of ovarian cancer in relation to the use of oral contraceptives.

    abstract::Female residents of six counties in Washington and Utah in whom a diagnosis of ovarian cancer was made during 1975-79 were interviewed concerning prior use of oral contraceptives. Interviews with a random sample of women drawn from these same counties were obtained for comparison. A smaller proportion of women with ep...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Weiss NS,Lyon JL,Liff JM,Vollmer WM,Daling JR

    更新日期:1981-12-01 00:00:00

  • Identification of microRNAs specifically expressed in hepatitis C virus-associated hepatocellular carcinoma.

    abstract::Although several studies have investigated the association of miRNAs with hepatocellular carcinoma (HCC), the data published so far are not concordant. A reason for these discrepancies may be the fact that most studies used the nontumorous tissue surrounding the HCC lesion as a control, which is almost invariably affe...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Diaz G,Melis M,Tice A,Kleiner DE,Mishra L,Zamboni F,Farci P

    更新日期:2013-08-15 00:00:00

  • Risk factors for Kaposi's sarcoma among HHV-8 seropositive homosexual men with AIDS.

    abstract::Kaposi's sarcoma (KS) is a frequent complication of the acquired immunodeficiency syndrome (AIDS) in homosexual men. Risk factors for developing this malignancy are uncertain, other than immunosuppression and coinfection with human herpesvirus 8 (HHV-8). We therefore examined factors associated with KS in a cross-sect...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nawar E,Mbulaiteye SM,Gallant JE,Wohl DA,Ardini M,Hendershot T,Goedert JJ,Rabkin CS,AIDS Cancer Cohort (ACC) Study Collaborators.

    更新日期:2005-06-10 00:00:00

  • Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice.

    abstract::A novel intra-operative chemotherapy nude mouse model for human hepatocellular carcinoma (HCC) has been developed. Intra-peritoneal (i.p.) administration of 5-fluorouracil (5-FU) was begun 2 hr before hepatic resection of HCC and then continued post-operatively for 4 consecutive days. This regime, termed intra-hepatec...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rashidi B,An Z,Sun FX,Li X,Tang ZY,Moossa AR,Hoffman RM

    更新日期:2001-01-15 00:00:00

  • Endogenous sex hormones and the risk of prostate cancer: a prospective study.

    abstract::Sex steroid hormones influence prostate development and maintenance through their roles in prostate cellular proliferation, differentiation and apoptosis. Although suspected to be involved in prostate carcinogenesis, an association between circulating androgens and prostate cancer has not been clearly established in e...

    journal_title:International journal of cancer

    pub_type: 杂志文章,随机对照试验


    authors: Weiss JM,Huang WY,Rinaldi S,Fears TR,Chatterjee N,Hsing AW,Crawford ED,Andriole GL,Kaaks R,Hayes RB

    更新日期:2008-05-15 00:00:00

  • The decline in the incidence of stomach cancer in Sweden 1960-1984: a birth cohort phenomenon.

    abstract::Trends in the incidence of stomach cancer among 52,604 patients notified to the National Swedish Cancer Registry in 1960 through 1984 were analysed. Age-standardized incidence rates declined throughout the period, from 47.1 to 24.6 per 10(5) in males and from 23.8 to 12.7 per 10(5) in females. Among males the decline ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hansson LE,Bergström R,Sparén P,Adami HO

    更新日期:1991-02-20 00:00:00

  • Treatment of melanoma of unknown primary in the era of immunotherapy and targeted therapy: A Dutch population-based study.

    abstract::Melanoma of unknown primary (MUP) may have a different biology to melanoma of known primary, but clinical trials of novel therapies (e.g., immune checkpoint or BRAF/MEK inhibitors) have not reported the outcomes in this population. We therefore evaluated the overall survival (OS) among patients with MUP in the era of ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Verver D,van der Veldt A,van Akkooi A,Verhoef C,Grünhagen DJ,Louwman WJ

    更新日期:2020-01-01 00:00:00

  • Specific anti-fibroblast cytotoxicity of antibodies to fibroblast surface antigen.

    abstract::An antiserum to SF antigen was shown to be selectively cytotoxic to two human fibroblast lines, while two tumor cell lines (one melanoma and one Hela cell line) were resistant. The selective cytotoxicity was also demonstrable in mixtures of fibroblasts and tumor cells. The cytotoxic effect was abrogated by admixture o...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ruoslahti E,Sjögren HO

    更新日期:1976-09-15 00:00:00

  • IGFBP-3 prolongs the p53 response and enhances apoptosis following UV irradiation.

    abstract::Neoplastic transformation is characterised by an imbalance in favour of cell growth over programmed cell death (apoptosis). The tumour-suppressor gene p53, responsible for maintaining cell-cycle control, is mutated in the majority of human cancers. Loss of function of the target genes of p53 are therefore important in...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hollowood AD,Lai T,Perks CM,Newcomb PV,Alderson D,Holly JM

    更新日期:2000-11-01 00:00:00

  • Human leukocyte antigen G polymorphism is associated with an increased risk of invasive cancer of the uterine cervix.

    abstract::Human leukocyte antigen (HLA)-G acts as negative regulator of the immune responses and its expression in tumor cells may enable them to escape immunosurveillance. The purpose of this study was to investigate the influence of HLA-G polymorphism on risk of high-grade cervical intraepithelial neoplasia (HG-CIN) and cervi...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ferguson R,Ramanakumar AV,Koushik A,Coutlée F,Franco E,Roger M,Biomarkers of Cervical Cancer Risk Study Team.

    更新日期:2012-08-01 00:00:00

  • Statin use and head and neck squamous cell carcinoma outcomes.

    abstract::Head and neck squamous cell carcinoma (HNSCC) is a morbid cancer with poor outcomes. Statins possess anticancer properties such as immunomodulatory and anti-inflammatory effects. The objective of our study is to identify the association between statin use among untreated HNSCC patients and overall death, disease-speci...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Getz KR,Bellile E,Zarins KR,Rullman C,Chinn SB,Taylor JMG,Rozek LS,Wolf GT,Mondul AM

    更新日期:2020-12-15 00:00:00

  • Dietary intake of fish, ω-3 and ω-6 fatty acids and risk of colorectal cancer: A prospective study in U.S. men and women.

    abstract::The association between fish, ω-3 and ω-6 polyunsaturated fatty acid (PUFA) intake and risk of colorectal cancer (CRC) remains inconclusive. Recent prospective studies suggest that the relationship may vary by gender, subsite and duration of follow-up. We followed 123,529 US adults (76,386 women and 47,143 men) withou...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Song M,Chan AT,Fuchs CS,Ogino S,Hu FB,Mozaffarian D,Ma J,Willett WC,Giovannucci EL,Wu K

    更新日期:2014-11-15 00:00:00

  • Implementation of the 7th edition AJCC staging system: Effects on staging and survival for pT1 melanoma. A Dutch population based study.

    abstract::In the 7th edition of the AJCC staging system, the mitotic rate criterion replaced Clark level to increase correct classification of high-risk thin melanoma patients (pT1B). Additionally, sentinel node biopsy (SNB) was recommended for nodal staging of pT1B melanomas. The aim of this article was to evaluate the effects...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Oude Ophuis CM,Louwman MW,Grünhagen DJ,Verhoef K,van Akkooi AC

    更新日期:2017-04-15 00:00:00

  • Production and characterization of a mouse/human chimeric antibody directed against human neuroblastoma.

    abstract::Hybridoma CE7 produces a murine antibody (gamma 1/kappa) which binds to a 190-kDa cell-surface glycoprotein of human neuroblastoma. Because of its tumor specificity, it has been used routinely in clinical pathology to confirm diagnosis of neuroblastoma. We have isolated the gene segments coding for the variable region...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Amstutz H,Rytz C,Novak-Hofer I,Spycher M,Schubiger PA,Blaser K,Morgenthaler JJ

    更新日期:1993-01-02 00:00:00

  • Interval cancers in the Norwegian breast cancer screening program: frequency, characteristics and use of HRT.

    abstract::Breast cancers diagnosed between screening examinations among women who attend a breast cancer screening program are defined as interval cancers. The Norwegian Breast Cancer Screening Program started as a pilot project in 1996, and data from the first 2-year interval are available. Our study quantifies interval cancer...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Wang H,Bjurstam N,Bjørndal H,Braaten A,Eriksen L,Skaane P,Vitak B,Hofvind S,Thoresen SØ

    更新日期:2001-11-01 00:00:00

  • Augmentation of B16 melanoma lung colony formation in C57BL/6 mice having marked granulocytosis.

    abstract::The effect that polymorphonuclear leukocytes (PMN) may have on the metastatic colonization of tumor cells is controversial: some laboratories have reported that PMN can inhibit metastasis whereas others have shown an augmentation effect. We have exploited our finding that a particular fibrosarcoma (BMT-11) transplante...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ishikawa M,Koga Y,Hosokawa M,Kobayashi H

    更新日期:1986-06-15 00:00:00

  • Nup88 mRNA overexpression is associated with high aggressiveness of breast cancer.

    abstract::The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Immunohistochemical studies have shown that this overexpression is linked to higher aggressiveness of colorectal carcinoma and to enhanced metastatic potential of melanoma cells. However, the antibodies so far developed against Nup88 have the draw...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Agudo D,Gómez-Esquer F,Martínez-Arribas F,Núñez-Villar MJ,Pollán M,Schneider J

    更新日期:2004-05-01 00:00:00

  • p53 mutation spectra for squamous cell carcinomas at different levels of human bronchial branches.

    abstract::The question of whether squamous cell carcinomas (SCCs) arising in different sites of lung are caused by different etiological factors is of obvious importance for prevention, early detection and effective treatment. We here subclassified a large series of resected SCCs (74 cases) into 3 tumor-sites, central (main to ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Shimmyo T,Hashimoto T,Kobayashi Y,Miyagi Y,Ishikawa Y,Nakagawa K,Osada H,Tsuchiya E

    更新日期:2006-08-01 00:00:00

  • Isolation and analysis of two strongly transforming isoforms of the Epstein-Barr-Virus(EBV)-encoded latent membrane protein-1 (LMP1) from a single Hodgkin's lymphoma.

    abstract::Two genes encoding the latent membrane protein 1 (LMP1) of the Epstein-Barr virus (EBV) were isolated from a single case of Hodgkin's disease (HD) and were tested for their biological activities. The LMP1 gene from the Reed-Sternberg cells contained point mutations relative to the prototype LMP1 gene, leading to amino...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Mehl AM,Fischer N,Rowe M,Hartmann F,Daus H,Trümper L,Pfreundschuh M,Müller-Lantzsch N,Grässer FA

    更新日期:1998-04-13 00:00:00