当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Protection by inhibition of poly (ADP-ribose) synthetase against oxidant injury in cardiac myoblasts In vitro.

Protection by inhibition of poly (ADP-ribose) synthetase against oxidant injury in cardiac myoblasts In vitro.

Abstract：

:Peroxynitrite and hydroxyl radical are reactive oxidants produced during myocardial reperfusion injury. In various cell types, including macrophages and smooth muscle cells, peroxynitrite and hydrogen peroxide cause DNA single strand breakage, which triggers the activation of the nuclear enzyme poly (ADP-ribose) synthetase (PARS), resulting in cytotoxicity. Using 3-aminobenzamide and nicotinamide, inhibitors of PARS, we investigated the role of PARS in the pathogenesis of myocardial oxidant injury in H9c2 cardiac myoblasts in vitro. Peroxynitrite (100-1000 microM), hydrogen peroxide (0.3-10 microM) and the NO donor compounds S-nitroso-N-accetyl-DL-penicillamine (SNAP) and diethyltriamine NONOate all caused a dose-dependent reduction of the mitochondrial respiration of the cells, as measured by the mitochondrial-dependent conversion of MTT to formazan. Peroxynitrite and hydrogen peroxide, but not the NO donors caused activation of cellular PARS activity. The suppression of mitochondrial respiration by peroxynitrite and hydrogen peroxide, but not by the NO donors, was ameliorated by pharmacological inhibition of PARS. The protection by the PARS inhibitors diminished at extremely high concentrations of the oxidants. Hypoxia (1 h) followed by reoxygenation (1-24 h) also resulted in a significant activation of PARS, and caused a suppression of mitochondrial respiration, which was prevented by inhibition of PARS. Similar to the results obtained with the pharmacological inhibitors of PARS, a fibroblast cell line which derives from the PARS knockout mouse was protected against the suppression of mitochondrial respiration in response to peroxynitrite and reoxygenation, but not to NO donors, when compared to the result of cells derived from wild-type animals. Based on our data, we suggest that activation of PARS plays a role in the myocardial oxidant injury.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Gilad E,Zingarelli B,Salzman AL,Szabó C

doi

10.1006/jmcc.1997.0496

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

2585-97

issue

9

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(97)90496-X

journal_volume

29

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Palmitoyl lysophosphatidylcholine mediated mobilization of LPL to the coronary luminal surface requires PKC activation.

    abstract：:Following diazoxide (DZ) induced hypoinsulinemia, cardiac luminal lipoprotein lipase (LPL) increases [Cardiovasc. Res. 3 (2003) 788]. To identify circulating mediators that maintain high LPL in vivo, DZ hearts were perfused for 1 h in the presence or absence of glucose, triglyceride (TG), palmitic acid or palmitoyl ly...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2004.07.003

    authors： Pulinilkunnil T,An D,Yip P,Chan N,Qi D,Ghosh S,Abrahani A,Rodrigues B

    更新日期：2004-11-01 00:00:00

  • HASF is a stem cell paracrine factor that activates PKC epsilon mediated cytoprotection.

    abstract：:Despite advances in the treatment of acute tissue ischemia significant challenges remain in effective cytoprotection from ischemic cell death. It has been documented that injected stem cells, such as mesenchymal stem cells (MSCs), can confer protection to ischemic tissue through the release of paracrine factors. The s...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.11.010

    authors： Huang J,Guo J,Beigi F,Hodgkinson CP,Facundo HT,Zhang Z,Espinoza-Derout J,Zhou X,Pratt RE,Mirotsou M,Dzau VJ

    更新日期：2014-01-01 00:00:00

  • Sulfhydryl modulation of K+ channels in rat ventricular myocytes.

    abstract：:Oxidative stress markedly alters protein function through redox modification of sulfhydryl groups present in cysteine residues. To explore the role of redox state in modulating cardiac K+ channels, this study examined the effects of sulfhydryl modifiers on the repolarizing transient outward current (Ito) in voltage-cl...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2002.2112

    authors： Rozanski GJ,Xu Z

    更新日期：2002-12-01 00:00:00

  • Effect of high free fatty acids on the anti-contractile response of perivascular adipose tissue in rat aorta.

    abstract：:To determine whether high free fatty acids (FFA) could affect the anti-contractile properties of perivascular adipose tissue (PVAT) in rat aortas. Wistar rats were divided into normal, obesity and fenofibrate groups and fed a normal, high-fat, and high-fat plus fenofibrate diet, respectively. Thoracic aortas with or w...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2013.07.018

    authors： Sun X,Hou N,Han F,Guo Y,Hui Z,Du G,Zhang Y

    更新日期：2013-10-01 00:00:00

  • Effects of protein kinase C inhibitors in in situ and isolated ischemic rabbit myocardium.

    abstract：:We tested the effects of the protein kinase C (PKC) inhibitors bisindolylmaleimide (1 microM) and chelerythrine (2 microM) on myocardial ischemia-reperfusion injury in in situ and isolated perfused rabbit hearts. In non-ischemic isolated hearts, bisindolylmaleimide (1 microM) and chelerythrine (2 microM) blocked sn-1,...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1997.0559

    authors： Lasley RD,Noble MA,Mentzer RM Jr

    更新日期：1997-12-01 00:00:00

  • Maturational differences in bioenergetic state and purine formation during "supply" and "demand" ischemia.

    abstract：:We examined metabolic effects of "supply" and "demand" ischemia in immature and mature rabbit hearts. Moderate supply ischemia was produced by a 50% reduction in coronary flow (to approximately 5.0 ml min-1 g-1 giving a 50-55% rise in O2 extraction and a 35% drop in O2 supply/demand). Demand ischemia was produced by s...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0105

    authors： Matherne GP,Ely SW,Headrick JP

    更新日期：1996-05-01 00:00:00

  • SUR, ABC proteins targeted by KATP channel openers.

    abstract：:The sulfonylurea receptor SUR is an ATP binding cassette (ABC) protein of the ABCC/MRP family. Unlike other ABC proteins, it has no intrinsic transport function, neither active nor passive, but associates with the potassium channel proteins Kir6.1 or Kir6.2 to form the ATP-sensitive potassium (K(ATP)) channel. Within ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2004.11.030

    authors： Moreau C,Prost AL,Dérand R,Vivaudou M

    更新日期：2005-06-01 00:00:00

  • DeltaPKC-mediated activation of epsilonPKC in ethanol-induced cardiac protection from ischemia.

    abstract：:Previous studies have demonstrated that acute ethanol exposure induces activation of delta protein kinase C (deltaPKC) and epsilonPKC, and mimics ischemic preconditioning via epsilonPKC activation. However, the role of deltaPKC isozyme in ischemia and reperfusion is still controversial. Here, we investigated the role ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.05.014

    authors： Inagaki K,Mochly-Rosen D

    更新日期：2005-08-01 00:00:00

  • Variations in the relative mRNA levels of actins and myosin heavy chains do not produce corresponding differences in their proteins in the adult human heart.

    abstract：:This paper examines the quantitative relationship between the expression of myosin heavy chain (MHC) and actin at both the levels of their mRNAs and their proteins. Explanted human left ventricle tissues were obtained from non-diseased (ND) individuals and from dilated cardiomyopathy (DCM) patients with terminally fai...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0317

    authors： Coumans JV,Yeoh T,Seeto RK,Keogh A,Brennan K,Gunning P,Hardeman E,dos Remedios CG

    更新日期：1997-03-01 00:00:00

  • HDAC inhibition induces autophagy and mitochondrial biogenesis to maintain mitochondrial homeostasis during cardiac ischemia/reperfusion injury.

    abstract：AIMS:The FDA-approved histone deacetylase (HDAC) inhibitor, suberoylanilide hydroxamic acid (SAHA, Vorinostat) has been shown to induce cardiomyocyte autophagy and blunt ischemia/reperfusion (I/R) injury when administered at the time of reperfusion. However, the precise mechanisms underlying the cardioprotective activi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2019.03.008

    authors： Yang J,He J,Ismail M,Tweeten S,Zeng F,Gao L,Ballinger S,Young M,Prabhu SD,Rowe GC,Zhang J,Zhou L,Xie M

    更新日期：2019-05-01 00:00:00

  • A caveolin-binding domain in the HCN4 channels mediates functional interaction with caveolin proteins.

    abstract：:Pacemaker (HCN) channels have a key role in the generation and modulation of spontaneous activity of sinoatrial node myocytes. Previous work has shown that compartmentation of HCN4 pacemaker channels within caveolae regulates important functions, but the molecular mechanism responsible is still unknown. HCN channels h...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.05.013

    authors： Barbuti A,Scavone A,Mazzocchi N,Terragni B,Baruscotti M,Difrancesco D

    更新日期：2012-08-01 00:00:00

  • Functional characterization of the novel DES mutation p.L136P associated with dilated cardiomyopathy reveals a dominant filament assembly defect.

    abstract：BACKGROUND:Dilated cardiomyopathy (DCM) could be caused by mutations in more than 40 different genes. However, the pathogenic impact of specific mutations is in most cases unknown complicating the genetic counseling of affected families. Therefore, functional studies could contribute to distinguish pathogenic mutations...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.12.015

    authors： Brodehl A,Dieding M,Biere N,Unger A,Klauke B,Walhorn V,Gummert J,Schulz U,Linke WA,Gerull B,Vorgert M,Anselmetti D,Milting H

    更新日期：2016-02-01 00:00:00

  • Ischemic preconditioning upregulates inducible nitric oxide synthase in cardiac myocyte.

    abstract：:Recent evidence has shown that the cardioprotection afforded by the late phase of ischemic preconditioning (PC) is mediated by upregulation of inducible nitric oxide synthase (iNOS). However, the specific cardiac cell type(s) that express(es) iNOS in response to ischemic PC remains unknown. Thus, mice underwent a sequ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1482

    authors： Wang Y,Guo Y,Zhang SX,Wu WJ,Wang J,Bao W,Bolli R

    更新日期：2002-01-01 00:00:00

  • Comparison of different iron chelators as protective agents against acute doxorubicin-induced cardiotoxicity.

    abstract：:Doxorubicin (Dox) is a widely used antineoplastic agent. Irreversible cardiomyopathy is a serious and dose-limiting side effect after chronic administration. The iron chelating bispiperazinedione ICRF-187 is currently the only drug which affords protection against Dox-induced cardiotoxicity. To compare the protective ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1136

    authors： Voest EE,van Acker SA,van der Vijgh WJ,van Asbeck BS,Bast A

    更新日期：1994-09-01 00:00:00

  • 7-Ketocholesterol predisposes human aorta smooth muscle cells to Fas-mediated death.

    abstract：:Human vascular smooth muscle cells (HVSMCs) are resistant to Fas-mediated death under normal physiological conditions. However, HVSMC death by activation of the receptor pathway was reported in the atherosclerotic lesions. In this study, we investigated whether 7-ketocholesterol, one of the major cholesterol oxides in...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2005.07.018

    authors： Rho MC,Kim YK,Chang JS,Lee HS,Baek JA,Chung MY,Lee HC,Lee HW,Rhim BY,Reidy MA,Kim K

    更新日期：2005-11-01 00:00:00

  • Atorvastatin-induced increase in progenitor cell levels is rather caused by enhanced receptor activator of NF-kappaB ligand (RANKL) cell proliferation than by bone marrow mobilization.

    abstract：BACKGROUND:Statins have been shown to increase the level of circulating progenitor cells in peripheral blood supposedly due to a mobilization of progenitor cells from the bone marrow niche. Osteoclast/osteoblast interaction has been associated with progenitor cell mobilization. Here, we investigated the role of statins...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.12.018

    authors： Steinmetz M,Pelster B,Lucanus E,Arnal JF,Nickenig G,Werner N

    更新日期：2013-04-01 00:00:00

  • Moderate calorie restriction improves cardiac remodeling and diastolic dysfunction in the Dahl-SS rat.

    abstract：:Caloric restriction extends longevity and reduces the onset of chronic disease in many animal models. Recently, caloric restriction was shown in humans to be associated with lower blood pressure, decreased systemic inflammation, and improved cardiac diastolic parameters. However, the causation and mechanisms of calori...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.07.012

    authors： Seymour EM,Parikh RV,Singer AA,Bolling SF

    更新日期：2006-10-01 00:00:00

  • Aerobic interval training enhances cardiomyocyte contractility and Ca2+ cycling by phosphorylation of CaMKII and Thr-17 of phospholamban.

    abstract：:Cardiac adaptation to aerobic exercise training includes improved cardiomyocyte contractility and calcium handling. Our objective was to determine whether cytosolic calcium/calmodulin-dependent kinase II and its downstream targets are modulated by exercise training. A six-week aerobic interval training program by trea...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.06.013

    authors： Kemi OJ,Ellingsen O,Ceci M,Grimaldi S,Smith GL,Condorelli G,Wisløff U

    更新日期：2007-09-01 00:00:00

  • Improvement of cholera toxin-catalyzed ADP-ribosylation by endogenous ADP-ribosylation factor from bovine brain provides evidence for an unchanged amount of Gs alpha in failing human myocardium.

    abstract：:The aim of the present study was to investigate whether or not alterations of Gs alpha can be detected with cholera toxin-induced ADP-ribosylation in myocardial membranes from patients with heart failure. Therefore, Gs alpha was radiolabeled by cholera toxin-catalzyed (32P)ADP-ribosylation with (32P)NAD as substrate. ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(90)90973-6

    authors： Schnabel P,Böhm M,Gierschik P,Jakobs KH,Erdmann E

    更新日期：1990-01-01 00:00:00

  • Phospholamban pentamers attenuate PKA-dependent phosphorylation of monomers.

    abstract：:Phospholamban (PLN) is a key regulator of cardiac contraction and relaxation through its inhibition of the sarco/endoplasmic reticulum Ca(2+)-ATPase (SERCA2a). The inhibitory effect is attenuated upon protein kinase A (PKA) dependent phosphorylation of PLN. PLN exists in an equilibrium of pentamers and monomers. While...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.12.020

    authors： Wittmann T,Lohse MJ,Schmitt JP

    更新日期：2015-03-01 00:00:00

  • Effects of neuropeptide Y on collateral development in a swine model of chronic myocardial ischemia.

    abstract：:We investigated the role of neuropeptide Y (NPY), abundant in the myocardial sympathetic nervous system and endothelial cells, in angiogenesis during chronic myocardial ischemia. Adult male Yorkshire swine underwent ameroid constrictor placement on the proximal left circumflex coronary artery. After 3 weeks, an osmoti...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2010.08.022

    authors： Robich MP,Matyal R,Chu LM,Feng J,Xu SH,Laham RJ,Hess PE,Bianchi C,Sellke FW

    更新日期：2010-12-01 00:00:00

  • Detection of TUNEL-positive cardiomyocytes and c-kit-positive progenitor cells in children with congenital heart disease.

    abstract：:The loss of cardiomyocytes by apoptosis and the subsequent replacement by fibrous connective tissues are important features of cardiac remodeling in adult heart disease. In children with CHD, however, the cellular and molecular mechanisms of heart failure have not yet been fully understood because of the anatomical an...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.05.011

    authors： Sato H,Shiraishi I,Takamatsu T,Hamaoka K

    更新日期：2007-09-01 00:00:00

  • Characterisation of murine cytomegalovirus myocarditis: cellular infiltration of the heart and virus persistence.

    abstract：:Myocarditis triggered by a viral infection has integral viral and immunological aspects associated with the pathogenesis of disease. The present study was performed to analyse the cellular inflammatory response in the heart and cytomegalovirus replication during the development of myocarditis in vivo. We examined muri...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2002.2003

    authors： Lenzo JC,Fairweather D,Cull V,Shellam GR,James Lawson CM

    更新日期：2002-06-01 00:00:00

  • Proteotoxicity: an underappreciated pathology in cardiac disease.

    abstract：:In general, in most organ systems, intracellular protein homeostasis is the sum of many factors, including chromosomal state, protein synthesis, post-translational processing and transport, folding, assembly and disassembly into macromolecular complexes, protein stability and clearance. In the heart, there has been a ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2013.12.015

    authors： Sandri M,Robbins J

    更新日期：2014-06-01 00:00:00

  • Prolonged Cre expression driven by the α-myosin heavy chain promoter can be cardiotoxic.

    abstract：:Studying the importance of genetic factors in a desired cell type or tissue necessitates the use of precise genetic tools. With the introduction of bacteriophage Cre recombinase/loxP mediated DNA editing and promoter-specific Cre expression, it is feasible to generate conditional knockout mice in which particular gene...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.06.019

    authors： Pugach EK,Richmond PA,Azofeifa JG,Dowell RD,Leinwand LA

    更新日期：2015-09-01 00:00:00

  • The effect of ouabain on hearts of cardiomyopathic hamsters: potentiation by isoproterenol.

    abstract：:The isometric twitch properties of papillary muscles from hearts of 30- to 53-day-old cardiomyopathic hamsters (BIO 14.6) were studied before and after exposure to the cardiac glycoside, ouabain. The diseased tissue was weakly responsive to ouabain (3 to 100 microM), as compared to a more appreciable positive inotropi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80368-1

    authors： Rossner KL,Mascarenhas DA

    更新日期：1987-06-01 00:00:00

  • Pathogenic mechanisms of pulmonary arterial hypertension.

    abstract：:Pulmonary arterial hypertension (PAH) is a complex disease that causes significant morbidity and mortality and is clinically characterized by an increase in pulmonary vascular resistance. The histopathology is marked by vascular proliferation/fibrosis, remodeling, and vessel obstruction. Development of PAH involves th...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2007.09.006

    authors： Chan SY,Loscalzo J

    更新日期：2008-01-01 00:00:00

  • Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

    abstract：:We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals we...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(95)91659-8

    authors： Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

    更新日期：1995-10-01 00:00:00

  • Characterization of thromboxane A2/prostaglandin H2 binding sites in guinea pig cardiac membrane preparations.

    abstract：:Thromboxane A2 (TXA2) and prostaglandin H2 (PGH2) induce platelet aggregation and are potent vasoconstrictors, and they have been implicated in coronary vasospasm and myocardial infarction. The TXA2 mimetic [1S-(1 alpha, 2 beta (5Z), 3 alpha (1E,3S*), 4 alpha)]-7-[3-(3-hydroxy-4-(4'- iodophenoxy)-1-butenyl)-7-oxabicyc...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1994.1109

    authors： Bowling N,Dubé GP,Kurtz WL,Brune KA,Saussy DL Jr,Dorn GW 2nd,Mais DE

    更新日期：1994-07-01 00:00:00

  • Effect of isoproterenol on myocardial mechanical function and cyclic AMP content in the fetal rabbit.

    abstract：:The effect of isoproterenol on mechanical function was studied in the isolated arterially perfused heart of the fetal (21st and 28th day of gestation) and newborn rabbits. The inotropic effect of isoproterenol in the fetus was less than in the newborn. In contrast, myocardial cyclic AMP levels after isoproterenol infu...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80557-6

    authors： Okuda H,Nakanishi T,Nakazawa M,Takao A

    更新日期：1987-02-01 00:00:00