当前位置： SCI文献检索 > JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY期刊下所有文献 > Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

Enrichment of G protein alpha-subunit mRNAS in the AV-conducting system of the mammalian heart.

Abstract：

:We investigated the expression pattern of the heterotrimeric G proteins Gs alpha, Gi alpha-2 and Go alpha in rat and guinea-pig heart by in situ hybridization. Cryosections were hybridized with single-stranded 35S-cRNA probes complementary to subtype-specific sequences of the respective mRNAs. Hybridization signals were visualized by exposition to X-ray films and dipping autoradiography. The rank order of abundance was Gi alpha-2 approximately Gs alpha > Go alpha. In general, G protein alpha-subunit mRNAs were evenly distributed in the heart including endo- and epicardium, large vessels and valves. Go alpha-mRNA levels were significantly higher in atria than in ventricles. In contrast to the rather uniform labeling of working myocardium, expression of all three G proteins was enriched in small intramural blood vessels and in subendocardial Purkinje fibers of septum and papillary muscles. A more marked enrichment of Gs alpha-, Gi alpha-2- and especially Go alpha-mRNA was seen in neuronal ganglionic cells in the atrial septum and posterior regions of the atrium. The main finding, however, was an enrichment of all three G protein mRNAs in the atrioventricular conductive tissue. The accumulation was strictly co-localized with acetylcholinesterase-positive regions identified as the atrioventricular node, the bundle of His and the right and left bundle branches and was seen similarly in rat and guinea-pig hearts. Quantitative in situ hybridization revealed Gs alpha-, Gi alpha-2- and Go alpha-mRNA levels in the bundle of His to be 206 +/- 0.13%. 191 +/- 0.15% and 165 +/- 0.06%, respectively, of that in the surrounding interventricular working myocardium. These findings indicate that heterotrimeric G proteins play an important role in modulation of electrical conductance in the heart.

journal_name

J Mol Cell Cardiol

journal_title

Journal of molecular and cellular cardiology

authors

Eschenhagen T,Laufs U,Schmitz W,Scholz H,Warnholtz A,Weil J,Schäfer HJ

doi

10.1016/s0022-2828(95)91659-8

subject

Has Abstract

pub_date

1995-10-01 00:00:00

pages

2249-63

issue

10

eissn

0022-2828

issn

1095-8584

pii

S0022-2828(95)91659-8

journal_volume

27

pub_type

杂志文章

相关文献

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY文献大全
  • Myocardium-targeted delivery of endothelial progenitor cells by ultrasound-mediated microbubble destruction improves cardiac function via an angiogenic response.

    abstract：:Application of ultrasound-mediated destruction of microbubbles (US + Bubble) to skeletal muscle creates capillary ruptures leading to leakage of the cell components. We studied whether US + Bubble combined with bone-marrow-derived mononuclear cells (BM-MNCs) infusion enables the targeted delivery of endothelial-lineag...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.03.012

    authors： Zen K,Okigaki M,Hosokawa Y,Adachi Y,Nozawa Y,Takamiya M,Tatsumi T,Urao N,Tateishi K,Takahashi T,Matsubara H

    更新日期：2006-06-01 00:00:00

  • Proteotoxicity: an underappreciated pathology in cardiac disease.

    abstract：:In general, in most organ systems, intracellular protein homeostasis is the sum of many factors, including chromosomal state, protein synthesis, post-translational processing and transport, folding, assembly and disassembly into macromolecular complexes, protein stability and clearance. In the heart, there has been a ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2013.12.015

    authors： Sandri M,Robbins J

    更新日期：2014-06-01 00:00:00

  • The basic helix-loop-helix transcription factor scleraxis regulates fibroblast collagen synthesis.

    abstract：:The transcription factor scleraxis has been implicated in regulating the development of collagen-rich tissues such as tendons and cardiac valves, but its role in general collagen synthesis in the heart is unknown. Scleraxis expression in cardiac fibroblasts was examined, and its ability to regulate gene expression of ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.03.024

    authors： Espira L,Lamoureux L,Jones SC,Gerard RD,Dixon IM,Czubryt MP

    更新日期：2009-08-01 00:00:00

  • Dioctanoyl-glycerol inhibits L-type calcium current in embryonic chick cardiomyocytes independent of protein kinase C activation.

    abstract：:Diacylglycerol analogs and phorbol esters are used as protein kinase C (PKC) activators to investigate the effect of PKC on L-type calcium current [ICa(L)] in cardiomyocytes. 1-2-dioctanoyl-sn-glycerol (diC8) is a potent analog of diacylglycerol (DAG) which produces positive inotropic effects in guinea-pig atria cardi...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(95)90058-6

    authors： Conforti L,Sumii K,Sperelakis N

    更新日期：1995-05-01 00:00:00

  • Mechanism of the positive inotropic effect of milrinone in cultured embryonic chick ventricular cells.

    abstract：:Milrinone, a potent positive inotropic and vasodilating agent, has shown promise in the clinical treatment of congestive heart failure, but significant controversy about its mechanism of action exists. To approach these mechanistic problems in a non-innervated, non-diffusion-limited system, the effects of milrinone on...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(87)80548-5

    authors： Olson EM,Kim D,Smith TW,Marsh JD

    更新日期：1987-01-01 00:00:00

  • A phenotypic screen to identify hypertrophy-modulating microRNAs in primary cardiomyocytes.

    abstract：:MicroRNAs (miRNAs) are small non-coding RNAs that control expression of complementary target mRNAs. A growing number of miRNAs has been implicated in the pathogenesis of cardiac diseases, mostly based not on functional data, but on the observation that they are dysregulated in diseased myocardium. Consequently, our kn...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2011.07.010

    authors： Jentzsch C,Leierseder S,Loyer X,Flohrschütz I,Sassi Y,Hartmann D,Thum T,Laggerbauer B,Engelhardt S

    更新日期：2012-01-01 00:00:00

  • Stimulation of DNA synthesis by PDGF in the newt cardiac myocyte.

    abstract：:The adult newt cardiac ventricular myocyte has been successfully placed in cell culture and has been shown to undergo in vitro DNA synthesis. Although several growth factors have been reported to increase DNA synthesis in cardiac myocytes in vitro, PDGF has not been reported to do so, but has been shown to be active i...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)91870-b

    authors： Soonpaa MH,Oberpriller JO,Oberpriller JC

    更新日期：1992-09-01 00:00:00

  • Mitochondrial instability during regional ischemia-reperfusion underlies arrhythmias in monolayers of cardiomyocytes.

    abstract：:Regional depolarization of the mitochondrial network can alter cellular electrical excitability and increase the propensity for reentry, in part, through the opening of sarcolemmal KATP channels. Mitochondrial inner membrane potential (ΔΨm) instability or oscillation can be induced in myocytes by exposure to reactive ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.09.024

    authors： Solhjoo S,O'Rourke B

    更新日期：2015-01-01 00:00:00

  • Role of 8-nitro-cGMP and its redox regulation in cardiovascular electrophilic signaling.

    abstract：:Structural and morphological changes of the cardiovascular systems (cardiovascular remodeling) are a major clinical outcome of cardiovascular diseases. Many lines of evidences have implied that transfiguration of reduction/oxidation (redox) homeostasis due to excess production of reactive oxygen species (ROS) and/or R...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2014.02.003

    authors： Nishida M,Toyama T,Akaike T

    更新日期：2014-08-01 00:00:00

  • Adenosine-mediated inhibition of 5'-AMP-activated protein kinase and p38 mitogen-activated protein kinase during reperfusion enhances recovery of left ventricular mechanical function.

    abstract：:Attenuation of excessive rates of myocardial glycolysis limits proton production and Ca(2+) overload during reperfusion and improves recovery of post-ischemic left ventricular (LV) function. In order to elucidate mechanisms underlying glycolytic inhibition by adenosine (ADO), this study tested the hypothesis that the ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.03.014

    authors： Omar MA,Verma S,Clanachan AS

    更新日期：2012-06-01 00:00:00

  • Circulating human CD34+ progenitor cells modulate neovascularization and inflammation in a nude mouse model.

    abstract：:CD34+ progenitor cells hold promise for therapeutic neovascularization in various settings. In this study, the role of human peripheral blood CD34+ cells in neovascularization and inflammatory cell recruitment was longitudinally studied in vivo. Human CD34+ cells were incorporated in Matrigel, implanted subcutaneously...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2007.03.907

    authors： van der Strate BW,Popa ER,Schipper M,Brouwer LA,Hendriks M,Harmsen MC,van Luyn MJ

    更新日期：2007-06-01 00:00:00

  • Programmed death-ligand 1 triggers PASMCs pyroptosis and pulmonary vascular fibrosis in pulmonary hypertension.

    abstract：:Pyroptosis is a pro-inflammatory form of programmed cell death, whose genesis directly depended on caspase-1 activation. Pulmonary hypertension (PH) is a disease characterized, in part, by vascular fibrosis. Up to now, there is no report on the relationship between pyroptosis and vascular fibrosis in PH. Here, we conf...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2019.10.008

    authors： Zhang M,Xin W,Yu Y,Yang X,Ma C,Zhang H,Liu Y,Zhao X,Guan X,Wang X,Zhu D

    更新日期：2020-01-01 00:00:00

  • The influence of lanthanum on the subcellular distribution of calcium in the perfused dog heart.

    abstract：:A newly developed method was used to make a direct study of the influence of lanthanum on the subcellular distribution of isotopic and elemental calcium in the isolated perfused dog heart. The trivalent cation was introduced into the heart for the last 5 min of a 60 min perfusion period. Developed pressure and dP/dt w...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80944-0

    authors： Kolbeck RC,Speir WA Jr

    更新日期：1986-07-01 00:00:00

  • Cross-talk between cardiac kappa-opioid and beta-adrenergic receptors in developing hypertensive rats.

    abstract：:The kappa-opioid receptor exerts a negative modulatory action on the beta-adrenoceptor and the action is blunted in adult spontaneously hypertensive rats (SHR). In order to determine whether the blunted negative modulation of the beta-adrenoceptor by the kappa-opioid receptor contributes to the development of hyperten...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1998.0896

    authors： Yu XC,Wang HX,Zhang WM,Wong TM

    更新日期：1999-03-01 00:00:00

  • Ablation of biglycan attenuates cardiac hypertrophy and fibrosis after left ventricular pressure overload.

    abstract：AIMS:Biglycan, a small leucine-rich proteoglycan, has been shown to play an important role in stabilizing fibrotic scars after experimental myocardial infarction. However, the role of biglycan in the development and regression of cardiomyocyte hypertrophy and fibrosis during cardiac pressure overload and unloading rema...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2016.10.011

    authors： Beetz N,Rommel C,Schnick T,Neumann E,Lother A,Monroy-Ordonez EB,Zeeb M,Preissl S,Gilsbach R,Melchior-Becker A,Rylski B,Stoll M,Schaefer L,Beyersdorf F,Stiller B,Hein L

    更新日期：2016-12-01 00:00:00

  • Diabetes and susceptibility to reperfusion-induced ventricular arrhythmias.

    abstract：:Studies using chemically-induced models of diabetes have shown the diabetic myocardium to exhibit abnormalities in cellular ion transport, which may affect susceptibility to reperfusion-induced arrhythmias. We studied the incidence of reperfusion-induced ventricular tachycardia (VT) and fibrillation (VF) in isolated h...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/0022-2828(92)93195-p

    authors： Kusama Y,Hearse DJ,Avkiran M

    更新日期：1992-04-01 00:00:00

  • Endothelium-targeted overexpression of constitutively active FGF receptor induces cardioprotection in mice myocardial infarction.

    abstract：:Fibroblast growth factor receptor (FGFR) is expressed in a variety of cells and is involved in their proliferation/migration/survival. To elucidate FGFR-mediated specific action of vascular endothelial cells (ECs) on myocardial ischemia, we generated endothelium-targeted transgenic mice overexpressing constitutively a...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.01.015

    authors： Matsunaga S,Okigaki M,Takeda M,Matsui A,Honsho S,Katsume A,Kishita E,Che J,Kurihara T,Adachi Y,Mansukhani A,Kobara M,Matoba S,Tatsumi T,Matsubara H

    更新日期：2009-05-01 00:00:00

  • Arrhythmogenic coupling between the Na+ -Ca2+ exchanger and inositol 1,4,5-triphosphate receptor in rat pulmonary vein cardiomyocytes.

    abstract：:Atrial fibrillation, the most common sustained arrhythmia, is believed to be triggered by ectopic electrical activity originating in the myocardial sleeves surrounding the pulmonary veins (PVs). It has been reported that myocardial sleeves have the potential to generate automaticity in response to norepinephrine. This...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2012.01.007

    authors： Okamoto Y,Takano M,Ohba T,Ono K

    更新日期：2012-05-01 00:00:00

  • An autocrine role for leptin in mediating the cardiomyocyte hypertrophic effects of angiotensin II and endothelin-1.

    abstract：:Leptin is a 16 kDa product of the obesity gene secreted primarily by adipocytes. We recently identified cardiomyocytes as a target for the direct hypertrophic effects of leptin and suggested that leptin may be a biological link between obesity and cardiovascular pathologies. Activation of the renin-angiotensin and end...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2006.05.001

    authors： Rajapurohitam V,Javadov S,Purdham DM,Kirshenbaum LA,Karmazyn M

    更新日期：2006-08-01 00:00:00

  • Alterations in the baroreceptor reflex control of heart rate in streptozotocin diabetic rats.

    abstract：:Baroreflex control of heart rate was studied in conscious diabetic rats at 12, 24 and 48 weeks after the induction of diabetes with streptozotocin. Baseline blood pressure (mean arterial blood pressure) of diabetic rats was significantly lower at 12 weeks after the induction of diabetes when compared to age-matched co...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/s0022-2828(86)80969-5

    authors： Chang KS,Lund DD

    更新日期：1986-06-01 00:00:00

  • Targeting cardiac fibroblasts to treat fibrosis of the heart: focus on HDACs.

    abstract：:Cardiac fibrosis is implicated in numerous physiologic and pathologic conditions, including scar formation, heart failure and cardiac arrhythmias. However the specific cells and signaling pathways mediating this process are poorly understood. Lysine acetylation of nucleosomal histone tails is an important mechanism fo...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.yjmcc.2014.02.015

    authors： Schuetze KB,McKinsey TA,Long CS

    更新日期：2014-05-01 00:00:00

  • Bradykinin protects against infarction but does not mediate ischemic preconditioning in the isolated rat heart.

    abstract：:The aim of the study was to test if pre-ischemic treatment with bradykinin can protect against infarction in an isolated rat heart model of regional ischemia and reperfusion, and if any such protection is dependent upon activation of protein kinase C (PKC) or mediated through the nitric oxide (NO) pathway. We also inv...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0226

    authors： Bugge E,Ytrehus K

    更新日期：1996-12-01 00:00:00

  • MYBPC3 truncation mutations enhance actomyosin contractile mechanics in human hypertrophic cardiomyopathy.

    abstract：RATIONALE:Truncation mutations in the MYBPC3 gene, encoding for cardiac myosin-binding protein C (MyBP-C), are the leading cause of hypertrophic cardiomyopathy (HCM). Whole heart, fiber and molecular studies demonstrate that MyBP-C is a potent modulator of cardiac contractility, but how these mutations contribute to HC...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2018.12.003

    authors： O'Leary TS,Snyder J,Sadayappan S,Day SM,Previs MJ

    更新日期：2019-02-01 00:00:00

  • Late preconditioning elicited by activation of adenosine A(3) receptor in heart: role of NF- kappa B, iNOS and mitochondrial K(ATP) channel.

    abstract：:Activation of adenosine A(3) receptor (A(3)AR) protects against ischemia/reperfusion injury in the heart. However, the downstream signaling mechanisms leading to its delayed anti-ischemic effects remain unclear. We hypothesized that A(3)AR stimulation protects the heart via activation of nuclear transcription factor k...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1510

    authors： Zhao TC,Kukreja RC

    更新日期：2002-03-01 00:00:00

  • Cytotoxic T lymphocytes are activated following myocardial infarction and can recognize and kill healthy myocytes in vitro.

    abstract：:The damage of myocardial infarction (MI) is often progressive. A possible mechanism for subsequent myocardial damage and heart failure after MI is immune response against cardiac self-antigens. The purpose of our study was to test the hypothesis that cytotoxic T lymphocytes are activated following acute MI and may hav...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2000.1261

    authors： Varda-Bloom N,Leor J,Ohad DG,Hasin Y,Amar M,Fixler R,Battler A,Eldar M,Hasin D

    更新日期：2000-12-01 00:00:00

  • NOR-1 modulates the inflammatory response of vascular smooth muscle cells by preventing NFκB activation.

    abstract：:Recent work has highlighted the role of NR4A receptors in atherosclerosis and inflammation. In vascular smooth muscle cell (VSMC) proliferation, however, NOR-1 (neuron-derived orphan receptor-1) exerts antagonistic effects to Nur77 and Nurr1. The aim of this study was to analyse the effect of NOR-1 in VSMC inflammator...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2014.12.015

    authors： Calvayrac O,Rodríguez-Calvo R,Martí-Pamies I,Alonso J,Ferrán B,Aguiló S,Crespo J,Rodríguez-Sinovas A,Rodríguez C,Martínez-González J

    更新日期：2015-03-01 00:00:00

  • Enhanced modification of cardiolipin during ischemia in the aged heart.

    abstract：:Aging enhances cardiac injury during ischemia and reperfusion compared to the adult heart, including in the Fischer 344 rat model of aging (F344). In interfibrillar cardiac mitochondria obtained from the elderly F344 rat, the rate of oxidative phosphorylation and the activity of electron transport complex III is decre...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2009.03.007

    authors： Lesnefsky EJ,Minkler P,Hoppel CL

    更新日期：2009-06-01 00:00:00

  • An in vitro model for the assessment of stem cell fate following implantation within the infarct microenvironment identifies ISL-1 expression as the strongest predictor of c-Kit(+) cardiac progenitor cells' therapeutic potential.

    abstract：:Cell therapy has the potential to drastically improve clinical outcomes for the 1.45 million patients suffering from a myocardial infarction (MI) each year in the U.S. However, the limitations associated with this treatment - including poor engraftment, significant cell death and poor differentiation potential - have ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1016/j.yjmcc.2015.09.007

    authors： Sullivan KE,Burns LJ,Black LD 3rd

    更新日期：2015-11-01 00:00:00

  • Effect of ATP depletion on kinetics of Na/Ca exchange-mediated Ca influx in Na-loaded heart cells.

    abstract：:The kinetics of Ca influx by Na/Ca exchange into adult rat heart cells loaded with Na and depleted of ATP were investigated, to further elucidate how ATP regulates exchanger properties in the intact heart cell. We found an eight-fold reduction in Vmax for Ca uptake by ATP depletion, with no significant change in the K...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.1996.0294

    authors： Haworth RA,Biggs AV

    更新日期：1997-02-01 00:00:00

  • Ischemic preconditioning upregulates inducible nitric oxide synthase in cardiac myocyte.

    abstract：:Recent evidence has shown that the cardioprotection afforded by the late phase of ischemic preconditioning (PC) is mediated by upregulation of inducible nitric oxide synthase (iNOS). However, the specific cardiac cell type(s) that express(es) iNOS in response to ischemic PC remains unknown. Thus, mice underwent a sequ...

    journal_title：Journal of molecular and cellular cardiology

    pub_type： 杂志文章

    doi：10.1006/jmcc.2001.1482

    authors： Wang Y,Guo Y,Zhang SX,Wu WJ,Wang J,Bao W,Bolli R

    更新日期：2002-01-01 00:00:00