Abstract:
:The heterocyclic aromatic amines, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) and 2-amino-3,4,8-trimethylimidazo[4,5-f]quinoxaline (4,8-DiMeIQx) are formed during frying of meat. PhIP and 4,8-DiMeIQx have, after metabolic activation, been shown to form adducts with DNA at the C8 of guanine both in vitro and in vivo. In order to investigate possible urinary biomarkers for estimation of the genotoxic dose of PhIP and 4,8-DiMeIQx, [3H]PhIP-dG, [3H]PhIP-DNA and [14C]4,8-DiMeIQx-DNA were injected i.p. to rats and the excretion of radioactivity in urine and faeces were measured. For all three [3H]PhIP-dG, [3H]PhIP-DNA and [14C]4,8-DiMeIQx-DNA 15-20% of the dose were excreted in the urine and 80-85% of the dose were excreted in the faeces. Urinary excretion showed maximum to 24 h (90%) with a rapid decline, 10% to 48 h and 0% to 72 h. Faecal excretion also showed maximum to 24 h (60%) with a slower decline, 30% to 48 h and 10% to 72 h. HPLC analysis of samples of urine and extracts from faeces, from rats dosed with [3H]PhIP-dG, showed that approximately 90% of the radioactivity co-eluted with PhIP-dG, indicating that PhIP-dG is excreted unmetabolized. HPLC analysis of samples of urine and extracts from faeces, from rats dosed with [3H]PhIP-DNA, showed that approximately 85% of the radioactivity co-eluted with PhIP-dG, indicating that PhIP-DNA adducts is mainly excreted as nucleoside adducts. Approximately 5% of the radioactivity excreted in the urine co-eluted with PhIP-G, indicating loss of deoxyribose. HPLC analysis of samples of urine and extracts from faeces, from rats dosed with [14C]4,8-DiMeIQx-DNA, showed that approximately 90% of the radioactivity co-eluted with 4,8-DiMeIQx-dG, indicating that 4,8-DiMeIQx-DNA adducts is mainly excreted as nucleoside adducts. Man is able to eliminate compounds of a higher mol. wt in the urine than the rat, the percentage of PhIP-dG and 4,8-DiMeIQx eliminated in the urine of man would therefore be expected to be higher than in the rat. Measurement of urinary nucleoside adducts of PhIP and 4,8-DiMeIQx could therefore provide a basis for the development of a biomonitoring strategy for the genotoxic dose of these food derived HAA.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Frandsen Hdoi
10.1093/carcin/18.8.1555subject
Has Abstractpub_date
1997-08-01 00:00:00pages
1555-60issue
8eissn
0143-3334issn
1460-2180journal_volume
18pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::In Saccharomyces cerevisiae a number of chemical agents induce synthesis of cytochrome P450. A cytochrome P450 gene has been well characterized in this yeast: CYP51, which codes for a constitutive enzyme involved in the 14 alpha-demethylation of lanosterol, a key step in the biosynthesis of ergosterol. In this work, w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/13.11.2175
更新日期:1992-11-01 00:00:00
abstract::DNA damage induced by tetrachlorohydroquinone (Cl(4)HQ), the quinonoid metabolite of pentachlorophenol (PCP), was investigated in human HeLa S3 tumor cells. Formation of one major and two minor DNA adducts in cells treated with Cl(4)HQ (50-300 microM) was detected by (32)P-post-labeling assay and the adducts accumulat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/22.4.635
更新日期:2001-04-01 00:00:00
abstract::Six N-nitroso compounds (N-nitrosodimethylamine, N-nitrosodiethylamine, N-nitrosodi-n-propylamine, N-nitroso-N-methylurea, N-nitrosopyrrolidine, and N-nitrosodi-n-butylamine) were tested in rats for liver DNA damaging activity by the alkaline elution technique. On molar basis their DNA damaging potency was found to de...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.5.425
更新日期:1981-01-01 00:00:00
abstract::We previously characterized a clone of CHO cells, clone B, that displayed tolerance to the cytotoxic effects of N-methylnitrosourea (MNU) and 6-thioguanine (6-TG). To determine whether this phenotype affected the mutagenic response of the cells, MNU-induced mutation to 8-azaadenine resistance (8-AAr) was measured in t...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.10.2097
更新日期:1993-10-01 00:00:00
abstract::Complementary DNA of marmoset CYP1A2 was isolated by means of screening the cDNA library and reverse-transcriptase polymerase chain reaction. The deduced amino acid sequence of marmoset CYP1A2 consisted of 516 residues and showed 88.2 and 90.0% identities to corresponding forms in human and cynomolgus monkey, respecti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/18.10.1985
更新日期:1997-10-01 00:00:00
abstract::Liver microsomes from male Syrian golden hamsters and Sprague Dawley rats metabolize the cis and trans isomers of N-nitroso-2,6-dimethylmorpholine (NNDM) to N-nitroso-(2-hydroxypropyl)(2-oxopropyl)amine (HPOP) as the major product detectable by h.p.l.c. The rates of total metabolism are similar for both the cis and tr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.8.1009
更新日期:1984-08-01 00:00:00
abstract::Structural analogs of diethylstilbestrol (DES) with at least one phenolic hydroxyl group are metabolized by prostaglandin H synthase (PHS) from ram seminal vesicle microsomes (RSVM) in vitro in the presence of arachidonic acid (20:4). U.v. spectroscopy revealed the formation of p-quinoid intermediates in incubations o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.1.137
更新日期:1986-01-01 00:00:00
abstract::Androgens and estrogens may play a role in gastric cancer etiology. To investigate the association of gastric cancer with single-nucleotide polymorphisms (SNPs) in six genes (COMT, CYP1B1, CYP17A1, CYP19A1, HSD17B1 and SHBG) involved in estrogen and androgen synthesis and metabolism, 58 haplotype-tagging SNPs were gen...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn258
更新日期:2009-01-01 00:00:00
abstract::Sodium arsenite and sodium arsenate were observed to induce morphological transformation of Syrian hamster embryo cells in a dose-dependent manner. A linear dose-dependence with a slope of approximately 1 was observed with both compounds when the data were plotted on a log-log graph. The trivalent sodium arsenite was ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.10.1421
更新日期:1985-10-01 00:00:00
abstract::Macrophages and their immortalized cell lines can be activated to form nitrite and nitrate via oxidation of arginine and this is accompanied by the formation of N-nitroso compounds. The mechanism of nitrosamine formation has been investigated through the use of compounds which are known either to inhibit or enhance ac...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.3.563
更新日期:1989-03-01 00:00:00
abstract::Metabolites of 15,16-dihydrocyclopenta[a]phenanthren-17-one and its 1- and 12-methyl homologues (all non-carcinogens) along with those from the 11-methyl and 11,12-dimethyl-17-ketones (carcinogens), produced in vitro by hepatic microsomes from methylcholanthrene induced rats, were separated by reverse phase h.p.l.c. I...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.8.1217
更新日期:1985-08-01 00:00:00
abstract::Zinc has been shown to be accumulated in N-methyl-N-nitrosourea (MNU)-induced rat mammary tumors. Zinc is required for cell proliferation and tumorigenesis is characterized by dysregulation of cell proliferation. An accumulation of zinc in mammary tumors perhaps indicates a reliance on zinc to sustain tumor growth. Li...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh214
更新日期:2004-10-01 00:00:00
abstract::The present study was aimed at verifying the occurrence, if any, of in vivo oxidative DNA damage in FA homozygotes, their parents and siblings. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) was measured, by HPLC/EC, in DNA from circulating blood leucocytes from FA homozygotes and their relatives and compared with a group of pa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.4.735
更新日期:1995-04-01 00:00:00
abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), a mutagen found in cooked meat, has been shown to induce mammary gland tumors in rats. Our laboratory recently observed that a high fat diet enhances the incidence and severity of PhIP-induced mammary gland cancer in rats. In the current study, reverse transcript...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.12.3087
更新日期:1995-12-01 00:00:00
abstract::Aminoacyl-transfer ribonucleic acid (tRNA) synthetases-interacting multifunctional protein (AIMP) 2 is a factor associated with the macromolecular protein synthesis machinery consisting of nine different aminoacyl-tRNA synthetases and three non-enzymatic factors. However, it was shown to work as a multifaceted regulat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp170
更新日期:2009-09-01 00:00:00
abstract::Syrian hamster embryo cells were treated with crocidolite asbestos at a dose (1 microgram/cm2) which was shown in previous studies to induce cell transformation and aneuploidy in these cells. Treatment of cells with asbestos induced a greater than 20-fold increase in the incidence of cells in anaphase with abnormaliti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.3.473
更新日期:1985-03-01 00:00:00
abstract::Chemical carcinogens are generally activated enzymatically to electrophiles that form covalently bound carcinogen-DNA adducts. Detoxifying enzymes are competing with the activating enzymes for these procarcinogenic chemical substrates. Wide person-to-person variations in these two types of enzymatic activities are fou...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/10.9.1563
更新日期:1989-09-01 00:00:00
abstract::Inactivation of the adenomatous polyposis coli (Apc) gene by loss of the wild-type Apc allele (LOH) is a prerequisite for the development of intestinal adenomas in Msh2 proficient Min (Apc+/-Msh2+/+) mice. In contrast, adenomas from Msh2 deficient Min (Apc+/-Msh2-/-) mice are not usually associated with LOH. Given the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/24.2.217
更新日期:2003-02-01 00:00:00
abstract::Garcinol, a polyisoprenylated benzophenone, from the fruit rind of Garcinia spp., has been shown to have anti-inflammatory and anticarcinogenic activities. To study its mechanism of action, we analyzed the effects of garcinol and its derivatives, including cambogin, garcim-1 and garcim-2, on arachidonic acid metabolis...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi208
更新日期:2006-02-01 00:00:00
abstract::Autoradiography has been used to study the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA), 4-O-methyl TPA, and phorbol on metabolic cooperation between human diploid fibroblasts. When the donors, hypoxanthine-guanine phosphoribosyl transferase+ (HGPRT+) cells, and recipients, HGPRT- cells, were plated together i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.10.1207
更新日期:1982-01-01 00:00:00
abstract::Inhalation of diesel exhaust (DE), which contains soot particles with adsorbed mutagenic organic compounds, and its virtually mutagen-free soot particle analog, carbon black (CB), produce similar types and prevalences of pulmonary neoplasms in chronically exposed F344 rats. This result suggests that DE-induced neoplas...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.5.1215
更新日期:1995-05-01 00:00:00
abstract::The chemopreventive effects of phenethyl isothiocyanate (PEITC) were investigated in N-nitrosobis(2-oxopropyl)-amine (BOP)-treated hamsters. Female 5-week-old Syrian golden hamsters were divided into six groups. Animals in groups 1-3, each consisting of 30 hamsters, were given BOP by two subcutaneous injections 7 days...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.6.1381
更新日期:1996-06-01 00:00:00
abstract::The association between prostate cancer risk and dietary fat consumption is well documented and explained partly by accelerated lipid peroxidation. We explored the possible effects of high dietary cholesterol on carcinogenesis and oxidative stress in the prostate of ACI/Seg rats. The rats develop prostate cancer spont...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh105
更新日期:2004-06-01 00:00:00
abstract:UNLABELLED:Mitogen-activated protein kinase-activated protein kinase 2 (MAPKAPK2) is recognized as oncogenic and simulative role on tumorigenesis by virtue of abnormal expression in cancer including nasopharyngeal carcinoma (NPC). We hypothesized that the copy number variation (CNV)-30450, which duplicates the MAPKAPK2...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt314
更新日期:2014-01-01 00:00:00
abstract::The Manchester (England) Children's Tumour Registry is population-based with a high level of ascertainment. Pathologic review ensured diagnostic accuracy. Routine monitoring of annual incidence revealed a significant increase in germ cell tumours from approximately 1 per million person years to approximately 4. Analys...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.1.75
更新日期:1982-01-01 00:00:00
abstract::Reactive oxygen species (ROS) are a major cause of cellular injury in an increasing number of diseases, including cancer. Most ROS are created in the cell through normal cellular metabolism. They can be produced by environmental insults such as ultraviolet light and toxic chemicals, as well as by the inflammatory proc...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgn142
更新日期:2008-09-01 00:00:00
abstract::The cyclooxygenase-2 (COX-2) inhibitor celecoxib and the ornithine decarboxylase (ODC) inhibitor difluoromethylornithine (DFMO) were each previously shown to prevent skin tumor development when administered throughout the course of UV irradiation. This raised the question of whether maintenance or continued growth of ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg046
更新日期:2003-05-01 00:00:00
abstract::The aim of this study has been to define cytotoxic mechanisms that may cause clonal expansion in the liver of pre-carcinogenic cells. An in vitro model, which has been described previously, was used. Hepatocytes were isolated from carcinogen-treated rats and a high proportion of the cells were gamma-glutamyltranspepti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.1.69
更新日期:1990-01-01 00:00:00
abstract::No previous study has examined the association between mitochondrial DNA copy number (mtCN) and skin cancer risk prospectively. We examined the associations between peripheral blood leukocytes mtCN level and the risks of skin cancers in a case-control study nested within the Nurses' Health Study of non-Hispanic White ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgw072
更新日期:2016-09-01 00:00:00
abstract::X-ray repair cross-complementing group 1 (XRCC1) and xeroderma pigmentosum group D (XPD) are mainly involved in base excision repair (BER) and nucleotide excision repair (NER) of DNA repair pathways, respectively. Polymorphisms of DNA repair gene XRCC1 and XPD has recently been identified, and there is a growing body ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.8.1321
更新日期:2002-08-01 00:00:00