Abstract：
:The influence of inflammatory cells on airway reactivity was investigated on arachidonic acid-induced relaxations of guinea-pig trachea and on arachidonic acid metabolism in guinea-pig tracheal epithelial cells. The presence of either eosinophils or neutrophils (1.0 x 10(7) cells/ml), from bronchoalveolar lavage, decreased the tracheal relaxations induced by arachidonic acid (1.0-30 microM). The basal synthesis of prostaglandin E2 was increased in epithelial cells (from 176 +/- 36 to 7920 +/- 898 pg/ml), eosinophils (from 360 +/- 56 to 2693 +/- 686 pg/ml) and neutrophils (from 352 +/- 81 to 4400 +/- 272 pg/ml) following incubation with arachidonic acid (10 microM). The co-incubation of either eosinophils or neutrophils with epithelial cells, in the presence of arachidonic acid, decreased the synthesis of prostaglandin E2 (2600 +/- 686 and 4400 +/- 272 pg/ml respectively) but increased the synthesis of thromboxane B2 (from 60 +/- 6 to 11634 +/- 840 and 9282 +/- 485 pg/ml respectively). Similarly, when major basic protein-treated (100 micrograms/ml) epithelial cells were incubated with arachidonic acid, the prostaglandin E2 synthesis decreased (75%) but thromboxane B2 synthesis was unaffected. The results suggest that eosinophils and neutrophils may impair arachidonic acid metabolism in guinea-pig epithelium in favor of production of bronchoconstrictor prostanoids.
journal_name
Eur J Pharmacoljournal_title
European journal of pharmacologyauthors
Prié S,Conroy DM,Gleich GJ,Sirois Pdoi
10.1016/s0014-2999(96)00736-4subject
Has Abstractpub_date
1996-12-19 00:00:00pages
343-51issue
2-3eissn
0014-2999issn
1879-0712pii
S0014-2999(96)00736-4journal_volume
317pub_type
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